Restriction map for
pF1KB5711
(Circular) (Six-base) MAPSORT of: pF1KB5711.seq Check: 1570 from: 1 to: 5468
pF1KB5711 5468 bp
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AccI GT'mk_AC
Cuts at: 683 2190 2506 683
Size: 1507 316 3645
Fragments arranged by size:
3645 1507 316
Acc65I G'GTAC_C
Cuts at: 2484 2484
Size: 5468
AclI AA'CG_TT
Cuts at: 5308 5308
Size: 5468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1647 3293 3725 4472 4740 1647
Size: 1646 432 747 268 2375
Fragments arranged by size:
2375 1646 747 432 268
AfeI AGC'GCT
Cuts at: 479 479
Size: 5468
AflIII A'CryG_T
Cuts at: 2429 2820 3924 4901 2429
Size: 391 1104 977 2996
Fragments arranged by size:
2996 1104 977 391
AgeI A'CCGG_T
Cuts at: 3856 3856
Size: 5468
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2321 2321
Size: 5468
AlwNI CAG_nnn'CTG
Cuts at: 853 2332 2773 4340 853
Size: 1479 441 1567 1981
Fragments arranged by size:
1981 1567 1479 441
ApaLI G'TGCA_C
Cuts at: 4238 4238
Size: 5468
ApoI r'AATT_y
Cuts at: 533 562 1549 2174 2233 2472 4821 5077 533
Size: 29 987 625 59 239 2349 256 924
Fragments arranged by size:
2349 987 924 625 256 239 59 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 5468
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
AvaI C'yCGr_G
Cuts at: 511 2488 511
Size: 1977 3491
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1234 1267 1234
Size: 33 5435
BamHI G'GATC_C
Cuts at: 2493 2493
Size: 5468
BanI G'GyrC_C
Cuts at: 2484 2898 3146 3181 2484
Size: 414 248 35 4771
Fragments arranged by size:
4771 414 248 35
BanII G_rGCy'C
Cuts at: 474 512 1229 1935 2018 2482 3512 474
Size: 38 717 706 83 464 1030 2430
Fragments arranged by size:
2430 1030 717 706 464 83 38
BbeI G_GCGC'C
Cuts at: 2902 3150 2902
Size: 248 5220
BbsI GAAGACnn'nnnn_
Cuts at: 2007 2007
Size: 5468
BciVI GTATCCnnnnn_n'
Cuts at: 3366 4127 5468 3366
Size: 761 1341 3366
Fragments arranged by size:
3366 1341 761
BclI T'GATC_A
Cuts at: 2373 2988 2373
Size: 615 4853
BglII A'GATC_T
Cuts at: 2983 5020 2983
Size: 2037 3431
BlpI GC'TnA_GC
Cuts at: 2580 2580
Size: 5468
Bme1580I G_kGCm'C
Cuts at: 3093 3186 4242 3093
Size: 93 1056 4319
Fragments arranged by size:
4319 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 419 2409 2680 2761 3096 5240 419
Size: 1990 271 81 335 2144 647
Fragments arranged by size:
2144 1990 647 335 271 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3835 3835
Size: 5468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 2153 298
Size: 1855 3613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 458 1475 2644 3523 4015 4313 180
Size: 278 1017 1169 879 492 298 1335
Fragments arranged by size:
1335 1169 1017 879 492 298 278
BsaI GGTCTCn'nnnn_
Cuts at: 1561 5172 1561
Size: 3611 1857
BsaAI yAC'GTr
Cuts at: 2821 2821
Size: 5468
BsaBI GATnn'nnATC
Cuts at: 6 2267 2982 5019 6
Size: 2261 715 2037 455
Fragments arranged by size:
2261 2037 715 455
BsaHI Gr'CG_yC
Cuts at: 2899 3147 3849 5345 2899
Size: 248 702 1496 3022
Fragments arranged by size:
3022 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1757 2657 3178 3856 4130 4277 4688 1757
Size: 900 521 678 274 147 411 2537
Fragments arranged by size:
2537 900 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 499 529 499
Size: 30 5438
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 460 2101 2270 460
Size: 1641 169 3658
Fragments arranged by size:
3658 1641 169
BseYI C'CCAG_C
Cuts at: 251 1589 2893 3651 4228 4724 251
Size: 1338 1304 758 577 496 995
Fragments arranged by size:
1338 1304 995 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2099 2099
Size: 5468
BsiEI CG_ry'CG
Cuts at: 104 3010 3056 4264 104
Size: 2906 46 1208 1308
Fragments arranged by size:
2906 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 474 2280 2482 3260 3450 4242 474
Size: 1806 202 778 190 792 1700
Fragments arranged by size:
1806 1700 792 778 202 190
Bsp1286I G_dGCh'C
Cuts at: 474 512 1229 1935 2018 2280 2482 3093 3186
Size: 38 717 706 83 262 202 611 93
Cuts at: 3186 3260 3450 3512 4242 474
Size: 74 190 62 730 1700
Fragments arranged by size:
1700 730 717 706 611 262 202 190
93 83 74 62 38
BspEI T'CCGG_A
Cuts at: 1757 4688 1757
Size: 2931 2537
BspHI T'CATG_A
Cuts at: 1311 4644 1311
Size: 3333 2135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1590 1894 1920 2518 2732 3034 3415 1590
Size: 304 26 598 214 302 381 3643
Fragments arranged by size:
3643 598 381 304 302 214 26
BsrBI CCG'CTC
Cuts at: 50 3760 3814 5298 5463 50
Size: 3710 54 1484 165 55
Fragments arranged by size:
3710 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1475 1896 3380 1475
Size: 421 1484 3563
Fragments arranged by size:
3563 1484 421
BsrFI r'CCGG_y
Cuts at: 3466 3647 3856 3466
Size: 181 209 5078
Fragments arranged by size:
5078 209 181
BssSI C'ACGA_G
Cuts at: 3739 4097 3739
Size: 358 5110
BstAPI GCAn_nnn'nTGC
Cuts at: 2845 2845
Size: 5468
BstBI TT'CG_AA
Cuts at: 3829 3829
Size: 5468
BstXI CCAn_nnnn'nTGG
Cuts at: 114 435 567 1425 114
Size: 321 132 858 4157
Fragments arranged by size:
4157 858 321 132
BstYI r'GATC_y
Cuts at: 1 1810 2493 2960 2983 3318 3564 4576 4662
Size: 1809 683 467 23 335 246 1012 86
Cuts at: 4662 4674 5020 1
Size: 12 346 449
Fragments arranged by size:
1809 1012 683 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 503 608 1556 503
Size: 105 948 4415
Fragments arranged by size:
4415 948 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3418 3599 3762 3418
Size: 181 163 5124
Fragments arranged by size:
5124 181 163
BtsI GCAGTG_nn'
Cuts at: 2711 2711
Size: 5468
DraI TTT'AAA
Cuts at: 2202 2415 2467 2202
Size: 213 52 5203
Fragments arranged by size:
5203 213 52
DrdI GACnn_nn'nnGTC
Cuts at: 3174 4032 3174
Size: 858 4610
EaeI y'GGCC_r
Cuts at: 950 1511 2442 3053 3227 3618 3645 4990 950
Size: 561 931 611 174 391 27 1345 1428
Fragments arranged by size:
1428 1345 931 611 561 391 174 27
EagI C'GGCC_G
Cuts at: 3053 3053
Size: 5468
EarI CTCTTCn'nnn_
Cuts at: 1245 1494 3491 3701 1245
Size: 249 1997 210 3012
Fragments arranged by size:
3012 1997 249 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3676 3986 4132 5278 3676
Size: 310 146 1146 3866
Fragments arranged by size:
3866 1146 310 146
EcoICRI GAG'CTC
Cuts at: 472 2480 472
Size: 2008 3460
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1647 2153 3293 3725 4472 4740 298
Size: 1349 506 1140 432 747 268 1026
Fragments arranged by size:
1349 1140 1026 747 506 432 268
EcoO109I rG'GnC_Cy
Cuts at: 233 614 2404 2607 233
Size: 381 1790 203 3094
Fragments arranged by size:
3094 1790 381 203
EcoRI G'AATT_C
Cuts at: 2472 2472
Size: 5468
EcoRV GAT'ATC
Cuts at: 543 543
Size: 5468
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2035 3487 2035
Size: 1452 4016
FspI TGC'GCA
Cuts at: 3249 3249
Size: 5468
HaeII r_GCGC'y
Cuts at: 481 2902 3150 4172 5151 481
Size: 2421 248 1022 979 798
Fragments arranged by size:
2421 1022 979 798 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 423 455 499 531 1158 1190 1467 1499 1624
Size: 32 44 32 627 32 277 32 125
Cuts at: 1624 1656 1795 1827 3310 3340 3342 3372 423
Size: 32 139 32 1483 30 2 30 2519
Fragments arranged by size:
2519 1483 627 277 139 125 44 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 873 1050 2191 2507 873
Size: 177 1141 316 3834
Fragments arranged by size:
3834 1141 316 177
HindIII A'AGCT_T
Cuts at: 316 316
Size: 5468
KasI G'GCGC_C
Cuts at: 2898 3146 2898
Size: 248 5220
KpnI G_GTAC'C
Cuts at: 2488 2488
Size: 5468
MfeI C'AATT_G
Cuts at: 1349 1349
Size: 5468
MluI A'CGCG_T
Cuts at: 4901 4901
Size: 5468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 497 1247 4139 4323 497
Size: 750 2892 184 1642
Fragments arranged by size:
2892 1642 750 184
MscI TGG'CCA
Cuts at: 952 1513 2444 3229 952
Size: 561 931 785 3191
Fragments arranged by size:
3191 931 785 561
MslI CAynn'nnrTG
Cuts at: 326 1849 3457 3584 326
Size: 1523 1608 127 2210
Fragments arranged by size:
2210 1608 1523 127
MspA1I CmG'CkG
Cuts at: 251 2579 2893 3253 4266 4511 251
Size: 2328 314 360 1013 245 1208
Fragments arranged by size:
2328 1208 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3649 3649
Size: 5468
NarI GG'CG_CC
Cuts at: 2899 3147 2899
Size: 248 5220
NcoI C'CATG_G
Cuts at: 1556 1556
Size: 5468
NdeI CA'TA_TG
Cuts at: 660 2304 660
Size: 1644 3824
NgoMIV G'CCGG_C
Cuts at: 3647 3647
Size: 5468
NspI r_CATG'y
Cuts at: 2433 2521 3021 3928 2433
Size: 88 500 907 3973
Fragments arranged by size:
3973 907 500 88
PciI A'CATG_T
Cuts at: 2429 3924 2429
Size: 1495 3973
PflMI CCAn_nnn'nTGG
Cuts at: 1645 1645
Size: 5468
PfoI T'CCnGG_A
Cuts at: 235 235
Size: 5468
PmeI GTTT'AAAC
Cuts at: 2467 2467
Size: 5468
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4639 4607
Size: 32 5436
PpuMI rG'GwC_Cy
Cuts at: 233 614 2404 233
Size: 381 1790 3297
Fragments arranged by size:
3297 1790 381
PshAI GACnn'nnGTC
Cuts at: 733 1573 733
Size: 840 4628
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2371 2403 2371
Size: 32 5436
PstI C_TGCA'G
Cuts at: 146 2300 2515 3200 146
Size: 2154 215 685 2414
Fragments arranged by size:
2414 2154 685 215
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5468
PvuII CAG'CTG
Cuts at: 251 2893 3253 251
Size: 2642 360 2466
Fragments arranged by size:
2642 2466 360
RsrII CG'GwC_CG
Cuts at: 3663 3663
Size: 5468
SacI G_AGCT'C
Cuts at: 474 2482 474
Size: 2008 3460
SalI G'TCGA_C
Cuts at: 2189 2505 2189
Size: 316 5152
SapI GCTCTTCn'nnn_
Cuts at: 3491 3701 3491
Size: 210 5258
SbfI CC_TGCA'GG
Cuts at: 2515 2515
Size: 5468
ScaI AGT'ACT
Cuts at: 4985 4985
Size: 5468
SfcI C'TryA_G
Cuts at: 33 142 684 935 1301 2296 2511 3196 4189
Size: 109 542 251 366 995 215 685 993
Cuts at: 4189 4380 33
Size: 191 1121
Fragments arranged by size:
1121 995 993 685 542 366 251 215
191 109
SfoI GGC'GCC
Cuts at: 2900 3148 2900
Size: 248 5220
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
SmaI CCC'GGG
Cuts at: 2490 2490
Size: 5468
SmlI C'TyrA_G
Cuts at: 195 473 1490 2623 3538 4030 4292 195
Size: 278 1017 1133 915 492 262 1371
Fragments arranged by size:
1371 1133 1017 915 492 278 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5468
SphI G_CATG'C
Cuts at: 2521 3021 2521
Size: 500 4968
SspI AAT'ATT
Cuts at: 1386 2055 1386
Size: 669 4799
StuI AGG'CCT
Cuts at: 454 1900 454
Size: 1446 4022
StyI C'CwwG_G
Cuts at: 1556 2602 2956 1556
Size: 1046 354 4068
Fragments arranged by size:
4068 1046 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 243 3051 3184 3643 3852 243
Size: 2808 133 459 209 1859
Fragments arranged by size:
2808 1859 459 209 133
TatI w'GTAC_w
Cuts at: 1001 1924 2456 4983 1001
Size: 923 532 2527 1486
Fragments arranged by size:
2527 1486 923 532
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4985 5017 4985
Size: 32 5436
Tth111I GACn'n_nGTC
Cuts at: 3265 3265
Size: 5468
XbaI T'CTAG_A
Cuts at: 2499 2499
Size: 5468
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 432 501 2963 432
Size: 69 2462 2937
Fragments arranged by size:
2937 2462 69
XmaI C'CCGG_G
Cuts at: 2488 2488
Size: 5468
XmnI GAAnn'nnTTC
Cuts at: 265 265
Size: 5468
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI NheI
NotI NruI NsiI PacI PasI PmlI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI