Restriction map for
pF1KB5709
(Circular) (Six-base) MAPSORT of: pF1KB5709.seq Check: 8945 from: 1 to: 5465
pF1KB5709 5465 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 2503 2503
Size: 5465
Acc65I G'GTAC_C
Cuts at: 2481 2481
Size: 5465
AclI AA'CG_TT
Cuts at: 5305 5305
Size: 5465
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 1743 2051 2160 2443 3290 3722 4469 4737
Size: 1556 308 109 283 847 432 747 268
Cuts at: 4737 187
Size: 915
Fragments arranged by size:
1556 915 847 747 432 308 283 268
109
AfeI AGC'GCT
Cuts at: 1936 1936
Size: 5465
AflIII A'CryG_T
Cuts at: 374 2817 3921 4898 374
Size: 2443 1104 977 941
Fragments arranged by size:
2443 1104 977 941
AgeI A'CCGG_T
Cuts at: 3853 3853
Size: 5465
AleI CACnn'nnGTG
Cuts at: 1900 1900
Size: 5465
AlwNI CAG_nnn'CTG
Cuts at: 2296 2770 4337 2296
Size: 474 1567 3424
Fragments arranged by size:
3424 1567 474
ApaI G_GGCC'C
Cuts at: 1408 1916 1976 1408
Size: 508 60 4897
Fragments arranged by size:
4897 508 60
ApaLI G'TGCA_C
Cuts at: 4235 4235
Size: 5465
ApoI r'AATT_y
Cuts at: 2469 4818 5074 2469
Size: 2349 256 2860
Fragments arranged by size:
2860 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5465
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5465
AvaI C'yCGr_G
Cuts at: 1367 2485 1367
Size: 1118 4347
BamHI G'GATC_C
Cuts at: 851 2490 851
Size: 1639 3826
BanI G'GyrC_C
Cuts at: 561 2481 2895 3143 3178 561
Size: 1920 414 248 35 2848
Fragments arranged by size:
2848 1920 414 248 35
BanII G_rGCy'C
Cuts at: 686 1408 1790 1916 1976 2306 2321 2479 3509
Size: 722 382 126 60 330 15 158 1030
Cuts at: 3509 686
Size: 2642
Fragments arranged by size:
2642 1030 722 382 330 158 126 60
15
BbeI G_GCGC'C
Cuts at: 2899 3147 2899
Size: 248 5217
BbsI GAAGACnn'nnnn_
Cuts at: 233 2006 2192 233
Size: 1773 186 3506
Fragments arranged by size:
3506 1773 186
BbvCI CC'TCA_GC
Cuts at: 1427 1643 2297 1427
Size: 216 654 4595
Fragments arranged by size:
4595 654 216
BciVI GTATCCnnnnn_n'
Cuts at: 3363 4124 5465 3363
Size: 761 1341 3363
Fragments arranged by size:
3363 1341 761
BclI T'GATC_A
Cuts at: 1178 2985 1178
Size: 1807 3658
BglII A'GATC_T
Cuts at: 1112 2980 5017 1112
Size: 1868 2037 1560
Fragments arranged by size:
2037 1868 1560
BlpI GC'TnA_GC
Cuts at: 2577 2577
Size: 5465
Bme1580I G_kGCm'C
Cuts at: 1408 1916 1976 2455 3090 3183 4239 1408
Size: 508 60 479 635 93 1056 2634
Fragments arranged by size:
2634 1056 635 508 479 93 60
BmrI ACTGGGnnnn_n'
Cuts at: 1837 2677 2758 3093 5237 1837
Size: 840 81 335 2144 2065
Fragments arranged by size:
2144 2065 840 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1992 3832 1992
Size: 1840 3625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 971 1342 1471 1804 1992 971
Size: 371 129 333 188 4444
Fragments arranged by size:
4444 371 333 188 129
Bpu10I CC'TnA_GC
Cuts at: 736 1427 1643 2297 736
Size: 691 216 654 3904
Fragments arranged by size:
3904 691 654 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1360 1437 2196 2641 3520 4012 4310 871
Size: 489 77 759 445 879 492 298 2026
Fragments arranged by size:
2026 879 759 492 489 445 298 77
BsaI GGTCTCn'nnnn_
Cuts at: 5169 5169
Size: 5465
BsaAI yAC'GTr
Cuts at: 2818 2818
Size: 5465
BsaBI GATnn'nnATC
Cuts at: 6 2979 5016 6
Size: 2973 2037 455
Fragments arranged by size:
2973 2037 455
BsaHI Gr'CG_yC
Cuts at: 2896 3144 3846 5342 2896
Size: 248 702 1496 3019
Fragments arranged by size:
3019 1496 702 248
BsaWI w'CCGG_w
Cuts at: 623 848 2654 3175 3853 4127 4274 4685 623
Size: 225 1806 521 678 274 147 411 1403
Fragments arranged by size:
1806 1403 678 521 411 274 225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2022 2052 2022
Size: 30 5435
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1474 1660 1663 2128 2131 2311 2314 1474
Size: 186 3 465 3 180 3 4625
Fragments arranged by size:
4625 465 186 180 3 3 3
BseYI C'CCAG_C
Cuts at: 754 1021 1328 1789 1917 2160 2198 2890 3648
Size: 267 307 461 128 243 38 692 758
Cuts at: 3648 4225 4721 754
Size: 577 496 1498
Fragments arranged by size:
1498 758 692 577 496 461 307 267
243 128 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 1225 1717 1093
Size: 132 492 4841
Fragments arranged by size:
4841 492 132
BsiEI CG_ry'CG
Cuts at: 104 2379 3007 3053 4261 104
Size: 2275 628 46 1208 1308
Fragments arranged by size:
2275 1308 1208 628 46
BsiHKAI G_wGCw'C
Cuts at: 686 742 920 2321 2479 3257 3447 4239 686
Size: 56 178 1401 158 778 190 792 1912
Fragments arranged by size:
1912 1401 792 778 190 178 158 56
Bsp1286I G_dGCh'C
Cuts at: 686 742 920 1408 1790 1916 1976 2306 2321
Size: 56 178 488 382 126 60 330 15
Cuts at: 2321 2455 2479 3090 3183 3257 3447 3509 4239
Size: 134 24 611 93 74 190 62 730
Cuts at: 4239 686
Size: 1912
Fragments arranged by size:
1912 730 611 488 382 330 190 178
134 126 93 74 62 60 56 24
15
BspEI T'CCGG_A
Cuts at: 623 4685 623
Size: 4062 1403
BspHI T'CATG_A
Cuts at: 4641 4641
Size: 5465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1580 1664 2515 2729 3031 3412 1580
Size: 84 851 214 302 381 3633
Fragments arranged by size:
3633 851 381 302 214 84
BsrBI CCG'CTC
Cuts at: 50 1372 1482 3757 3811 5295 5460 50
Size: 1322 110 2275 54 1484 165 55
Fragments arranged by size:
2275 1484 1322 165 110 55 54
BsrDI GCAATG_nn'
Cuts at: 3377 3377
Size: 5465
BsrFI r'CCGG_y
Cuts at: 3463 3644 3853 3463
Size: 181 209 5075
Fragments arranged by size:
5075 209 181
BsrGI T'GTAC_A
Cuts at: 862 862
Size: 5465
BssSI C'ACGA_G
Cuts at: 3736 4094 3736
Size: 358 5107
BstAPI GCAn_nnn'nTGC
Cuts at: 1680 2842 1680
Size: 1162 4303
BstBI TT'CG_AA
Cuts at: 3826 3826
Size: 5465
BstEII G'GTnAC_C
Cuts at: 2230 2230
Size: 5465
BstXI CCAn_nnnn'nTGG
Cuts at: 394 2016 394
Size: 1622 3843
BstYI r'GATC_y
Cuts at: 1 171 851 1112 1493 1827 2490 2957 2980
Size: 170 680 261 381 334 663 467 23
Cuts at: 2980 3315 3561 4573 4659 4671 5017 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 680 663 467 449 381 346 335
334 261 246 170 86 23 12
BtgI C'CryG_G
Cuts at: 107 777 1301 1532 2015 107
Size: 670 524 231 483 3557
Fragments arranged by size:
3557 670 524 483 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3415 3596 3759 3415
Size: 181 163 5121
Fragments arranged by size:
5121 181 163
BtsI GCAGTG_nn'
Cuts at: 500 639 819 867 1231 2132 2377 2708 500
Size: 139 180 48 364 901 245 331 3257
Fragments arranged by size:
3257 901 364 331 245 180 139 48
ClaI AT'CG_AT
Cuts at: 2336 2336
Size: 5465
DraI TTT'AAA
Cuts at: 2464 2464
Size: 5465
DrdI GACnn_nn'nnGTC
Cuts at: 235 583 3171 4029 235
Size: 348 2588 858 1671
Fragments arranged by size:
2588 1671 858 348
EaeI y'GGCC_r
Cuts at: 1373 1926 2376 2435 3050 3224 3615 3642 4987
Size: 553 450 59 615 174 391 27 1345
Cuts at: 4987 1373
Size: 1851
Fragments arranged by size:
1851 1345 615 553 450 391 174 59
27
EagI C'GGCC_G
Cuts at: 2376 3050 2376
Size: 674 4791
EarI CTCTTCn'nnn_
Cuts at: 1288 1842 2015 2099 2309 3488 3698 1288
Size: 554 173 84 210 1179 210 3055
Fragments arranged by size:
3055 1179 554 210 210 173 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3673 3983 4129 5275 3673
Size: 310 146 1146 3863
Fragments arranged by size:
3863 1146 310 146
EcoICRI GAG'CTC
Cuts at: 684 2319 2477 684
Size: 1635 158 3672
Fragments arranged by size:
3672 1635 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 971 1342 1471 1743 1804 1992 2051 2160
Size: 784 371 129 272 61 188 59 109
Cuts at: 2160 2443 3290 3722 4469 4737 187
Size: 283 847 432 747 268 915
Fragments arranged by size:
915 847 784 747 432 371 283 272
268 188 129 109 61 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 2386 2045
Size: 341 5124
EcoO109I rG'GnC_Cy
Cuts at: 329 1137 1404 1405 1653 1913 1972 1973 2604
Size: 808 267 1 248 260 59 1 631
Cuts at: 2604 329
Size: 3190
Fragments arranged by size:
3190 808 631 267 260 248 59 1
1
EcoRI G'AATT_C
Cuts at: 2469 2469
Size: 5465
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1131 3484 1131
Size: 2353 3112
FspI TGC'GCA
Cuts at: 1555 3246 1555
Size: 1691 3774
FspAI rTGC'GCAy
Cuts at: 1555 1555
Size: 5465
HaeII r_GCGC'y
Cuts at: 1938 2899 3147 4169 5148 1938
Size: 961 248 1022 979 2255
Fragments arranged by size:
2255 1022 979 961 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 396 428 782 814 1375 1407 1819
Size: 32 201 32 354 32 561 32 412
Cuts at: 1819 1851 2394 2426 3307 3337 3339 3369 163
Size: 32 543 32 881 30 2 30 2259
Fragments arranged by size:
2259 881 561 543 412 354 201 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 573 975 2504 573
Size: 402 1529 3534
Fragments arranged by size:
3534 1529 402
KasI G'GCGC_C
Cuts at: 2895 3143 2895
Size: 248 5217
KpnI G_GTAC'C
Cuts at: 2485 2485
Size: 5465
MluI A'CGCG_T
Cuts at: 4898 4898
Size: 5465
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 443 1676 4136 4320 443
Size: 1233 2460 184 1588
Fragments arranged by size:
2460 1588 1233 184
MscI TGG'CCA
Cuts at: 1928 2437 3226 1928
Size: 509 789 4167
Fragments arranged by size:
4167 789 509
MslI CAynn'nnrTG
Cuts at: 1000 1052 1900 3454 3581 1000
Size: 52 848 1554 127 2884
Fragments arranged by size:
2884 1554 848 127 52
MspA1I CmG'CkG
Cuts at: 857 1534 1581 1662 1671 1782 2576 2890 3250
Size: 677 47 81 9 111 794 314 360
Cuts at: 3250 4263 4508 857
Size: 1013 245 1814
Fragments arranged by size:
1814 1013 794 677 360 314 245 111
81 47 9
NaeI GCC'GGC
Cuts at: 3646 3646
Size: 5465
NarI GG'CG_CC
Cuts at: 2896 3144 2896
Size: 248 5217
NcoI C'CATG_G
Cuts at: 107 777 2015 107
Size: 670 1238 3557
Fragments arranged by size:
3557 1238 670
NgoMIV G'CCGG_C
Cuts at: 3644 3644
Size: 5465
NotI GC'GGCC_GC
Cuts at: 2376 2376
Size: 5465
NspI r_CATG'y
Cuts at: 378 1051 1956 2518 3018 3925 378
Size: 673 905 562 500 907 1918
Fragments arranged by size:
1918 907 905 673 562 500
PasI CC'CwG_GG
Cuts at: 127 127
Size: 5465
PciI A'CATG_T
Cuts at: 374 3921 374
Size: 3547 1918
PflMI CCAn_nnn'nTGG
Cuts at: 604 1904 1924 604
Size: 1300 20 4145
Fragments arranged by size:
4145 1300 20
PfoI T'CCnGG_A
Cuts at: 827 1630 827
Size: 803 4662
PmeI GTTT'AAAC
Cuts at: 2464 2464
Size: 5465
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4604 4636 4604
Size: 32 5433
PpuMI rG'GwC_Cy
Cuts at: 329 1137 1653 329
Size: 808 516 4141
Fragments arranged by size:
4141 808 516
PspOMI G'GGCC_C
Cuts at: 1404 1912 1972 1404
Size: 508 60 4897
Fragments arranged by size:
4897 508 60
PstI C_TGCA'G
Cuts at: 179 1172 1565 1586 1661 1676 2049 2330 2512
Size: 993 393 21 75 15 373 281 182
Cuts at: 2512 3197 179
Size: 685 2447
Fragments arranged by size:
2447 993 685 393 373 281 182 75
21 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5465
PvuII CAG'CTG
Cuts at: 1581 1671 1782 2890 3250 1581
Size: 90 111 1108 360 3796
Fragments arranged by size:
3796 1108 360 111 90
RsrII CG'GwC_CG
Cuts at: 3660 3660
Size: 5465
SacI G_AGCT'C
Cuts at: 686 2321 2479 686
Size: 1635 158 3672
Fragments arranged by size:
3672 1635 158
SacII CC_GC'GG
Cuts at: 1535 1535
Size: 5465
SalI G'TCGA_C
Cuts at: 2502 2502
Size: 5465
SanDI GG'GwC_CC
Cuts at: 329 329
Size: 5465
SapI GCTCTTCn'nnn_
Cuts at: 1842 2309 3488 3698 1842
Size: 467 1179 210 3609
Fragments arranged by size:
3609 1179 467 210
SbfI CC_TGCA'GG
Cuts at: 2512 2512
Size: 5465
ScaI AGT'ACT
Cuts at: 4982 4982
Size: 5465
SfcI C'TryA_G
Cuts at: 33 175 1035 1168 1561 1582 1657 1672 2045
Size: 142 860 133 393 21 75 15 373
Cuts at: 2045 2326 2508 3193 4186 4377 33
Size: 281 182 685 993 191 1121
Fragments arranged by size:
1121 993 860 685 393 373 281 191
182 142 133 75 21 15
SfoI GGC'GCC
Cuts at: 2897 3145 2897
Size: 248 5217
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5465
SmaI CCC'GGG
Cuts at: 2487 2487
Size: 5465
SmlI C'TyrA_G
Cuts at: 886 1339 1367 1416 2211 2620 3535 4027 4289
Size: 453 28 49 795 409 915 492 262
Cuts at: 4289 886
Size: 2062
Fragments arranged by size:
2062 915 795 492 453 409 262 49
28
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5465
SphI G_CATG'C
Cuts at: 1956 2518 3018 1956
Size: 562 500 4403
Fragments arranged by size:
4403 562 500
StyI C'CwwG_G
Cuts at: 107 333 565 698 777 1121 1526 2015 2599
Size: 226 232 133 79 344 405 489 584
Cuts at: 2599 2953 107
Size: 354 2619
Fragments arranged by size:
2619 584 489 405 354 344 232 226
133 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 757 780 813 1736 1822 3048 3181 3640 3849
Size: 23 33 923 86 1226 133 459 209
Cuts at: 3849 757
Size: 2373
Fragments arranged by size:
2373 1226 923 459 209 133 86 33
23
TatI w'GTAC_w
Cuts at: 862 2087 4980 862
Size: 1225 2893 1347
Fragments arranged by size:
2893 1347 1225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 4982 5014 2019
Size: 32 2931 32 2470
Fragments arranged by size:
2931 2470 32 32
Tth111I GACn'n_nGTC
Cuts at: 1746 3262 1746
Size: 1516 3949
XbaI T'CTAG_A
Cuts at: 2496 2496
Size: 5465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1383 2960 114
Size: 1269 1577 2619
Fragments arranged by size:
2619 1577 1269
XhoI C'TCGA_G
Cuts at: 1367 1367
Size: 5465
XmaI C'CCGG_G
Cuts at: 2485 2485
Size: 5465
XmnI GAAnn'nnTTC
Cuts at: 613 2140 613
Size: 1527 3938
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsmBI BssHII BstZ17I Bsu36I CspCI DraIII EcoRV FseI
HindIII HpaI MfeI NdeI NheI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI