Restriction map for
pF1KB5706
(Circular) (Six-base) MAPSORT of: pF1KB5706.seq Check: 6437 from: 1 to: 5264
pF1KB5706 5264 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1608 2302 1608
Size: 694 4570
Acc65I G'GTAC_C
Cuts at: 2280 2280
Size: 5264
AclI AA'CG_TT
Cuts at: 5104 5104
Size: 5264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3089 3521 4268 4536 3089
Size: 432 747 268 3817
Fragments arranged by size:
3817 747 432 268
AflIII A'CryG_T
Cuts at: 759 2616 3720 4697 759
Size: 1857 1104 977 1326
Fragments arranged by size:
1857 1326 1104 977
AgeI A'CCGG_T
Cuts at: 3652 3652
Size: 5264
AlwNI CAG_nnn'CTG
Cuts at: 308 957 2569 4136 308
Size: 649 1612 1567 1436
Fragments arranged by size:
1612 1567 1436 649
ApaI G_GGCC'C
Cuts at: 1566 2097 1566
Size: 531 4733
ApaLI G'TGCA_C
Cuts at: 4034 4034
Size: 5264
ApoI r'AATT_y
Cuts at: 1255 2030 2268 4617 4873 1255
Size: 775 238 2349 256 1646
Fragments arranged by size:
2349 1646 775 256 238
AseI AT'TA_AT
Cuts at: 20 20
Size: 5264
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5264
AvaI C'yCGr_G
Cuts at: 1496 2284 1496
Size: 788 4476
BamHI G'GATC_C
Cuts at: 2289 2289
Size: 5264
BanI G'GyrC_C
Cuts at: 816 2280 2694 2942 2977 816
Size: 1464 414 248 35 3103
Fragments arranged by size:
3103 1464 414 248 35
BanII G_rGCy'C
Cuts at: 1566 2097 2278 3308 1566
Size: 531 181 1030 3522
Fragments arranged by size:
3522 1030 531 181
BbeI G_GCGC'C
Cuts at: 2698 2946 2698
Size: 248 5016
BbsI GAAGACnn'nnnn_
Cuts at: 268 268
Size: 5264
BciVI GTATCCnnnnn_n'
Cuts at: 3162 3923 5264 3162
Size: 761 1341 3162
Fragments arranged by size:
3162 1341 761
BclI T'GATC_A
Cuts at: 647 2784 647
Size: 2137 3127
BglII A'GATC_T
Cuts at: 2779 4816 2779
Size: 2037 3227
BlpI GC'TnA_GC
Cuts at: 1763 2376 1763
Size: 613 4651
Bme1580I G_kGCm'C
Cuts at: 1566 2097 2889 2982 4038 1566
Size: 531 792 93 1056 2792
Fragments arranged by size:
2792 1056 792 531 93
BmgBI CAC'GTC
Cuts at: 760 760
Size: 5264
BmrI ACTGGGnnnn_n'
Cuts at: 279 287 996 1783 1933 2476 2557 2892 5036
Size: 8 709 787 150 543 81 335 2144
Cuts at: 5036 279
Size: 507
Fragments arranged by size:
2144 787 709 543 507 335 150 81
8
BmtI G_CTAG'C
Cuts at: 1771 2107 1771
Size: 336 4928
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 911 3631 911
Size: 2720 2544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 1571 1889 687
Size: 884 318 4062
Fragments arranged by size:
4062 884 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 821 1030 2440 3319 3811 4109 461
Size: 360 209 1410 879 492 298 1616
Fragments arranged by size:
1616 1410 879 492 360 298 209
BsaI GGTCTCn'nnnn_
Cuts at: 4968 4968
Size: 5264
BsaAI yAC'GTr
Cuts at: 2617 2617
Size: 5264
BsaBI GATnn'nnATC
Cuts at: 6 1706 2778 4815 6
Size: 1700 1072 2037 455
Fragments arranged by size:
2037 1700 1072 455
BsaHI Gr'CG_yC
Cuts at: 2695 2943 3645 5141 2695
Size: 248 702 1496 2818
Fragments arranged by size:
2818 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1782 2453 2974 3652 3926 4073 4484 1782
Size: 671 521 678 274 147 411 2562
Fragments arranged by size:
2562 678 671 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2192 2222 2192
Size: 30 5234
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 812 824 1970 812
Size: 12 1146 4106
Fragments arranged by size:
4106 1146 12
BseYI C'CCAG_C
Cuts at: 966 1410 2689 3447 4024 4520 966
Size: 444 1279 758 577 496 1710
Fragments arranged by size:
1710 1279 758 577 496 444
BsiEI CG_ry'CG
Cuts at: 104 2806 2852 4060 104
Size: 2702 46 1208 1308
Fragments arranged by size:
2702 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2238 2278 3056 3246 4038 2238
Size: 40 778 190 792 3464
Fragments arranged by size:
3464 792 778 190 40
BsmI GAATG_Cn'
Cuts at: 215 756 1639 215
Size: 541 883 3840
Fragments arranged by size:
3840 883 541
BsmBI CGTCTCn'nnnn_
Cuts at: 110 110
Size: 5264
Bsp1286I G_dGCh'C
Cuts at: 1566 2097 2238 2278 2889 2982 3056 3246 3308
Size: 531 141 40 611 93 74 190 62
Cuts at: 3308 4038 1566
Size: 730 2792
Fragments arranged by size:
2792 730 611 531 190 141 93 74
62 40
BspEI T'CCGG_A
Cuts at: 1782 4484 1782
Size: 2702 2562
BspHI T'CATG_A
Cuts at: 2220 4440 2220
Size: 2220 3044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2314 2528 2830 3211 2314
Size: 214 302 381 4367
Fragments arranged by size:
4367 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3556 3610 5094 5259 50
Size: 3506 54 1484 165 55
Fragments arranged by size:
3506 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3176 3176
Size: 5264
BsrFI r'CCGG_y
Cuts at: 3262 3443 3652 3262
Size: 181 209 4874
Fragments arranged by size:
4874 209 181
BsrGI T'GTAC_A
Cuts at: 317 2007 317
Size: 1690 3574
BssSI C'ACGA_G
Cuts at: 3535 3893 3535
Size: 358 4906
BstAPI GCAn_nnn'nTGC
Cuts at: 2641 2641
Size: 5264
BstBI TT'CG_AA
Cuts at: 3625 3625
Size: 5264
BstYI r'GATC_y
Cuts at: 1 577 2289 2756 2779 3114 3360 4372 4458
Size: 576 1712 467 23 335 246 1012 86
Cuts at: 4458 4470 4816 1
Size: 12 346 449
Fragments arranged by size:
1712 1012 576 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1609 1609
Size: 5264
BtgI C'CryG_G
Cuts at: 125 2126 125
Size: 2001 3263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3214 3395 3558 3214
Size: 181 163 4920
Fragments arranged by size:
4920 181 163
BtsI GCAGTG_nn'
Cuts at: 2507 2507
Size: 5264
DraI TTT'AAA
Cuts at: 1302 2263 1302
Size: 961 4303
DrdI GACnn_nn'nnGTC
Cuts at: 2970 3828 2970
Size: 858 4406
EaeI y'GGCC_r
Cuts at: 2849 3023 3414 3441 4786 2849
Size: 174 391 27 1345 3327
Fragments arranged by size:
3327 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2849 2849
Size: 5264
EarI CTCTTCn'nnn_
Cuts at: 179 609 683 902 1151 1643 3287 3497 179
Size: 430 74 219 249 492 1644 210 1946
Fragments arranged by size:
1946 1644 492 430 249 219 210 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3472 3782 3928 5074 3472
Size: 310 146 1146 3662
Fragments arranged by size:
3662 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2276 2276
Size: 5264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 1571 1889 3089 3521 4268 4536 687
Size: 884 318 1200 432 747 268 1415
Fragments arranged by size:
1415 1200 884 747 432 318 268
EcoNI CCTnn'n_nnAGG
Cuts at: 385 1480 385
Size: 1095 4169
EcoO109I rG'GnC_Cy
Cuts at: 1999 2093 2094 2403 1999
Size: 94 1 309 4860
Fragments arranged by size:
4860 309 94 1
EcoRI G'AATT_C
Cuts at: 2268 2268
Size: 5264
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3283 3283
Size: 5264
FspI TGC'GCA
Cuts at: 3045 3045
Size: 5264
HaeII r_GCGC'y
Cuts at: 2698 2946 3968 4947 2698
Size: 248 1022 979 3015
Fragments arranged by size:
3015 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 714 746 2192 2224 3106 3136 3138 3168 714
Size: 32 1446 32 882 30 2 30 2810
Fragments arranged by size:
2810 1446 882 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2303 2303
Size: 5264
KasI G'GCGC_C
Cuts at: 2694 2942 2694
Size: 248 5016
KpnI G_GTAC'C
Cuts at: 2284 2284
Size: 5264
MfeI C'AATT_G
Cuts at: 1211 1211
Size: 5264
MluI A'CGCG_T
Cuts at: 4697 4697
Size: 5264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3935 4119 3935
Size: 184 5080
MscI TGG'CCA
Cuts at: 3025 3025
Size: 5264
MslI CAynn'nnrTG
Cuts at: 1340 1478 3253 3380 1340
Size: 138 1775 127 3224
Fragments arranged by size:
3224 1775 138 127
MspA1I CmG'CkG
Cuts at: 139 607 1954 2375 2689 3049 4062 4307 139
Size: 468 1347 421 314 360 1013 245 1096
Fragments arranged by size:
1347 1096 1013 468 421 360 314 245
NaeI GCC'GGC
Cuts at: 3445 3445
Size: 5264
NarI GG'CG_CC
Cuts at: 2695 2943 2695
Size: 248 5016
NdeI CA'TA_TG
Cuts at: 195 426 483 1143 195
Size: 231 57 660 4316
Fragments arranged by size:
4316 660 231 57
NgoMIV G'CCGG_C
Cuts at: 3443 3443
Size: 5264
NheI G'CTAG_C
Cuts at: 1767 2103 1767
Size: 336 4928
NspI r_CATG'y
Cuts at: 2317 2817 3724 2317
Size: 500 907 3857
Fragments arranged by size:
3857 907 500
PciI A'CATG_T
Cuts at: 3720 3720
Size: 5264
PmeI GTTT'AAAC
Cuts at: 2263 2263
Size: 5264
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 4403
Size: 32 5232
PpuMI rG'GwC_Cy
Cuts at: 1999 1999
Size: 5264
PsiI TTA'TAA
Cuts at: 922 1745 922
Size: 823 4441
PspOMI G'GGCC_C
Cuts at: 1562 2093 1562
Size: 531 4733
PstI C_TGCA'G
Cuts at: 1353 1953 2311 2996 1353
Size: 600 358 685 3621
Fragments arranged by size:
3621 685 600 358
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5264
PvuII CAG'CTG
Cuts at: 139 607 1954 2689 3049 139
Size: 468 1347 735 360 2354
Fragments arranged by size:
2354 1347 735 468 360
RsrII CG'GwC_CG
Cuts at: 3459 3459
Size: 5264
SacI G_AGCT'C
Cuts at: 2278 2278
Size: 5264
SalI G'TCGA_C
Cuts at: 2301 2301
Size: 5264
SapI GCTCTTCn'nnn_
Cuts at: 3287 3497 3287
Size: 210 5054
SbfI CC_TGCA'GG
Cuts at: 2311 2311
Size: 5264
ScaI AGT'ACT
Cuts at: 2144 4781 2144
Size: 2637 2627
SexAI A'CCwGG_T
Cuts at: 1923 1923
Size: 5264
SfcI C'TryA_G
Cuts at: 33 245 572 1349 1371 1805 1949 2108 2307
Size: 212 327 777 22 434 144 159 199
Cuts at: 2307 2992 3985 4176 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 777 685 434 327 212 199
191 159 144 22
SfoI GGC'GCC
Cuts at: 2696 2944 2696
Size: 248 5016
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5264
SmaI CCC'GGG
Cuts at: 2286 2286
Size: 5264
SmlI C'TyrA_G
Cuts at: 476 836 1009 2419 3334 3826 4088 476
Size: 360 173 1410 915 492 262 1652
Fragments arranged by size:
1652 1410 915 492 360 262 173
SpeI A'CTAG_T
Cuts at: 67 1677 67
Size: 1610 3654
SphI G_CATG'C
Cuts at: 2317 2817 2317
Size: 500 4764
SspI AAT'ATT
Cuts at: 702 1366 1590 702
Size: 664 224 4376
Fragments arranged by size:
4376 664 224
StyI C'CwwG_G
Cuts at: 440 1232 2150 2398 2752 440
Size: 792 918 248 354 2952
Fragments arranged by size:
2952 918 792 354 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 525 2847 2980 3439 3648 525
Size: 2322 133 459 209 2141
Fragments arranged by size:
2322 2141 459 209 133
TatI w'GTAC_w
Cuts at: 317 2007 2142 4779 317
Size: 1690 135 2637 802
Fragments arranged by size:
2637 1690 802 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4781 4813 4781
Size: 32 5232
Tth111I GACn'n_nGTC
Cuts at: 3061 3061
Size: 5264
XbaI T'CTAG_A
Cuts at: 2295 2295
Size: 5264
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2759 2759
Size: 5264
XmaI C'CCGG_G
Cuts at: 2284 2284
Size: 5264
XmnI GAAnn'nnTTC
Cuts at: 1181 1181
Size: 5264
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NspI PciI
PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI Bpu10I
BsgI BsiWI BssHII BstEII BstXI Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI NcoI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI