(Circular) (Six-base) MAPSORT of: pF1KB5700.seq  Check: 6467  from: 1  to: 4391



pF1KB5700  4391 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AccI GT'mk_AC

Cuts at:   1429    1429 
   Size:       4391
 
Acc65I G'GTAC_C

Cuts at:   1407    1407 
   Size:       4391
 
AclI AA'CG_TT

Cuts at:   4231    4231 
   Size:       4391
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1169    2216    2648    3395    3663    1169 
   Size:       1047     432     747     268    1897

  Fragments arranged by size: 

                1897    1047     747     432     268
 
AflIII A'CryG_T

Cuts at:   1743    2847    3824    1743 
   Size:       1104     977    2310

  Fragments arranged by size: 

                2310    1104     977
 
AgeI A'CCGG_T

Cuts at:   2779    2779 
   Size:       4391
 
AlwNI CAG_nnn'CTG

Cuts at:   1696    3263    1696 
   Size:       1567    2824
 
ApaLI G'TGCA_C

Cuts at:   3161    3161 
   Size:       4391
 
ApoI r'AATT_y

Cuts at:    259     794     994    1395    3744    4000     259 
   Size:        535     200     401    2349     256     650

  Fragments arranged by size: 

                2349     650     535     401     256     200
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4391
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4391
 
AvaI C'yCGr_G

Cuts at:   1411    1411 
   Size:       4391
 
AvrII C'CTAG_G

Cuts at:    834     834 
   Size:       4391
 
BamHI G'GATC_C

Cuts at:   1416    1416 
   Size:       4391
 
BanI G'GyrC_C

Cuts at:   1407    1821    2069    2104    1407 
   Size:        414     248      35    3694

  Fragments arranged by size: 

                3694     414     248      35
 
BanII G_rGCy'C

Cuts at:   1405    2435    1405 
   Size:       1030    3361
 
BbeI G_GCGC'C

Cuts at:   1825    2073    1825 
   Size:        248    4143
 
BbsI GAAGACnn'nnnn_

Cuts at:    188     188 
   Size:       4391
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4357
 
BciVI GTATCCnnnnn_n'

Cuts at:   2289    3050    4391    2289 
   Size:        761    1341    2289

  Fragments arranged by size: 

                2289    1341     761
 
BclI T'GATC_A

Cuts at:    971    1911     971 
   Size:        940    3451
 
BglII A'GATC_T

Cuts at:    807    1906    3943     807 
   Size:       1099    2037    1255

  Fragments arranged by size: 

                2037    1255    1099
 
BlpI GC'TnA_GC

Cuts at:   1503    1503 
   Size:       4391
 
Bme1580I G_kGCm'C

Cuts at:   2016    2109    3165    2016 
   Size:         93    1056    3242

  Fragments arranged by size: 

                3242    1056      93
 
BmgBI CAC'GTC

Cuts at:    280     280 
   Size:       4391
 
BmrI ACTGGGnnnn_n'

Cuts at:   1603    1684    2019    4163    1603 
   Size:         81     335    2144    1831

  Fragments arranged by size: 

                2144    1831     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2758    2758 
   Size:       4391
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    508     725    1241    1352     508 
   Size:        217     516     111    3547

  Fragments arranged by size: 

                3547     516     217     111
 
Bpu10I CC'TnA_GC

Cuts at:    128     128 
   Size:       4391
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1567    2446    2938    3236    1567 
   Size:        879     492     298    2722

  Fragments arranged by size: 

                2722     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4095    4095 
   Size:       4391
 
BsaAI yAC'GTr

Cuts at:   1744    1744 
   Size:       4391
 
BsaBI GATnn'nnATC

Cuts at:      6    1319    1905    3942       6 
   Size:       1313     586    2037     455

  Fragments arranged by size: 

                2037    1313     586     455
 
BsaHI Gr'CG_yC

Cuts at:   1822    2070    2772    4268    1822 
   Size:        248     702    1496    1945

  Fragments arranged by size: 

                1945    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1580    2101    2779    3053    3200    3611    1580 
   Size:        521     678     274     147     411    2360

  Fragments arranged by size: 

                2360     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1816    2574    3151    3647    1816 
   Size:        758     577     496    2560

  Fragments arranged by size: 

                2560     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    419     419 
   Size:       4391
 
BsiEI CG_ry'CG

Cuts at:    104    1933    1979    3187     104 
   Size:       1829      46    1208    1308

  Fragments arranged by size: 

                1829    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1405    2183    2373    3165    1405 
   Size:        778     190     792    2631

  Fragments arranged by size: 

                2631     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    424     424 
   Size:       4391
 
Bsp1286I G_dGCh'C

Cuts at:   1405    2016    2109    2183    2373    2435    3165    1405 
   Size:        611      93      74     190      62     730    2631

  Fragments arranged by size: 

                2631     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3611    3611 
   Size:       4391
 
BspHI T'CATG_A

Cuts at:   3567    3567 
   Size:       4391
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1441    1655    1957    2338    1441 
   Size:        214     302     381    3494

  Fragments arranged by size: 

                3494     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2683    2737    4221    4386      50 
   Size:       2633      54    1484     165      55

  Fragments arranged by size: 

                2633    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2303    2303 
   Size:       4391
 
BsrFI r'CCGG_y

Cuts at:   2389    2570    2779    2389 
   Size:        181     209    4001

  Fragments arranged by size: 

                4001     209     181
 
BsrGI T'GTAC_A

Cuts at:   1115    1115 
   Size:       4391
 
BssSI C'ACGA_G

Cuts at:   2662    3020    2662 
   Size:        358    4033
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1768    1768 
   Size:       4391
 
BstBI TT'CG_AA

Cuts at:   2752    2752 
   Size:       4391
 
BstYI r'GATC_y

Cuts at:      1     402     807    1416    1883    1906    2241    2487    3499 
   Size:        401     405     609     467      23     335     246    1012

Cuts at:   3499    3585    3597    3943       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     609     467     449     405     401     346     335
                 246      86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2341    2522    2685    2341 
   Size:        181     163    4047

  Fragments arranged by size: 

                4047     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1634    1634 
   Size:       4391
 
DraI TTT'AAA

Cuts at:   1390    1390 
   Size:       4391
 
DraIII CAC_nnn'GTG

Cuts at:    295     295 
   Size:       4391
 
DrdI GACnn_nn'nnGTC

Cuts at:   2097    2955    2097 
   Size:        858    3533
 
EaeI y'GGCC_r

Cuts at:   1976    2150    2541    2568    3913    1976 
   Size:        174     391      27    1345    2454

  Fragments arranged by size: 

                2454    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1976    1976 
   Size:       4391
 
EarI CTCTTCn'nnn_

Cuts at:    773    1253    2414    2624     773 
   Size:        480    1161     210    2540

  Fragments arranged by size: 

                2540    1161     480     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    166     628    2599    2909    3055    4201     166 
   Size:        462    1971     310     146    1146     356

  Fragments arranged by size: 

                1971    1146     462     356     310     146
 
EcoICRI GAG'CTC

Cuts at:   1403    1403 
   Size:       4391
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    508     725    1169    1241    1352    2216    2648    3395    3663 
   Size:        217     444      72     111     864     432     747     268

Cuts at:   3663     508 
   Size:       1236

  Fragments arranged by size: 

                1236     864     747     444     432     268     217     111
                  72
 
EcoO109I rG'GnC_Cy

Cuts at:   1530    1530 
   Size:       4391
 
EcoRI G'AATT_C

Cuts at:   1395    1395 
   Size:       4391
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1232    2410    1232 
   Size:       1178    3213
 
FspI TGC'GCA

Cuts at:   2172    2172 
   Size:       4391
 
HaeII r_GCGC'y

Cuts at:   1825    2073    3095    4074    1825 
   Size:        248    1022     979    2142

  Fragments arranged by size: 

                2142    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    394     426     963     995    2233    2263    2265    2295     394 
   Size:         32     537      32    1238      30       2      30    2490

  Fragments arranged by size: 

                2490    1238     537      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    268    1430     268 
   Size:       1162    3229
 
HindIII A'AGCT_T

Cuts at:   1214    1214 
   Size:       4391
 
KasI G'GCGC_C

Cuts at:   1821    2069    1821 
   Size:        248    4143
 
KpnI G_GTAC'C

Cuts at:   1411    1411 
   Size:       4391
 
MluI A'CGCG_T

Cuts at:   3824    3824 
   Size:       4391
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3062    3246    3062 
   Size:        184    4207
 
MscI TGG'CCA

Cuts at:   2152    2152 
   Size:       4391
 
MslI CAynn'nnrTG

Cuts at:   2380    2507    2380 
   Size:        127    4264
 
MspA1I CmG'CkG

Cuts at:   1502    1816    2176    3189    3434    1502 
   Size:        314     360    1013     245    2459

  Fragments arranged by size: 

                2459    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2572    2572 
   Size:       4391
 
NarI GG'CG_CC

Cuts at:   1822    2070    1822 
   Size:        248    4143
 
NgoMIV G'CCGG_C

Cuts at:   2570    2570 
   Size:       4391
 
NspI r_CATG'y

Cuts at:    726    1444    1944    2851     726 
   Size:        718     500     907    2266

  Fragments arranged by size: 

                2266     907     718     500
 
PasI CC'CwG_GG

Cuts at:   1053    1053 
   Size:       4391
 
PciI A'CATG_T

Cuts at:   2847    2847 
   Size:       4391
 
PfoI T'CCnGG_A

Cuts at:    656     744     656 
   Size:         88    4303
 
PmeI GTTT'AAAC

Cuts at:   1390    1390 
   Size:       4391
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    810     842    3530    3562     810 
   Size:         32    2688      32    1639

  Fragments arranged by size: 

                2688    1639      32      32
 
PstI C_TGCA'G

Cuts at:   1438    2123    1438 
   Size:        685    3706
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4391
 
PvuII CAG'CTG

Cuts at:   1816    2176    1816 
   Size:        360    4031
 
RsrII CG'GwC_CG

Cuts at:   2586    2586 
   Size:       4391
 
SacI G_AGCT'C

Cuts at:   1405    1405 
   Size:       4391
 
SalI G'TCGA_C

Cuts at:   1428    1428 
   Size:       4391
 
SapI GCTCTTCn'nnn_

Cuts at:   2414    2624    2414 
   Size:        210    4181
 
SbfI CC_TGCA'GG

Cuts at:   1438    1438 
   Size:       4391
 
ScaI AGT'ACT

Cuts at:   3908    3908 
   Size:       4391
 
SfcI C'TryA_G

Cuts at:     33     368     413    1151    1434    2119    3112    3303      33 
   Size:        335      45     738     283     685     993     191    1121

  Fragments arranged by size: 

                1121     993     738     685     335     283     191      45
            
 
SfoI GGC'GCC

Cuts at:   1823    2071    1823 
   Size:        248    4143
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4391
 
SmaI CCC'GGG

Cuts at:   1413    1413 
   Size:       4391
 
SmlI C'TyrA_G

Cuts at:   1546    2461    2953    3215    1546 
   Size:        915     492     262    2722

  Fragments arranged by size: 

                2722     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67     966      67 
   Size:        899    3492
 
SphI G_CATG'C

Cuts at:   1444    1944    1444 
   Size:        500    3891
 
StyI C'CwwG_G

Cuts at:    834    1525    1879     834 
   Size:        691     354    3346

  Fragments arranged by size: 

                3346     691     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1066    1974    2107    2566    2775    1066 
   Size:        908     133     459     209    2682

  Fragments arranged by size: 

                2682     908     459     209     133
 
TatI w'GTAC_w

Cuts at:    445    1115    3906     445 
   Size:        670    2791     930

  Fragments arranged by size: 

                2791     930     670
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3908    3940    3908 
   Size:         32    4359
 
Tth111I GACn'n_nGTC

Cuts at:   2188    2188 
   Size:       4391
 
XbaI T'CTAG_A

Cuts at:    861    1422     861 
   Size:        561    3830
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1060    1886    1060 
   Size:        826    3565
 
XmaI C'CCGG_G

Cuts at:   1411    1411 
   Size:       4391
 
XmnI GAAnn'nnTTC

Cuts at:    869     869 
   Size:       4391
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PasI     PciI     PfoI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI     BaeI    BbvCI    BfrBI     BglI     BmtI    BsaXI
    BseRI    BsiWI    BsmBI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI    EcoNI    EcoRV     FseI    FspAI     HpaI
     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
    PflMI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     ZraI