Restriction map for
pF1KB5695
(Circular) (Six-base) MAPSORT of: pF1KB5695.seq Check: 7827 from: 1 to: 5021
pF1KB5695 5021 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 2059 2059
Size: 5021
Acc65I G'GTAC_C
Cuts at: 2037 2037
Size: 5021
AclI AA'CG_TT
Cuts at: 4861 4861
Size: 5021
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1307 2846 3278 3631 4293 1307
Size: 1539 432 353 662 2035
Fragments arranged by size:
2035 1539 662 432 353
AflII C'TTAA_G
Cuts at: 727 759 934 727
Size: 32 175 4814
Fragments arranged by size:
4814 175 32
AflIII A'CryG_T
Cuts at: 269 2373 4173 4454 269
Size: 2104 1800 281 836
Fragments arranged by size:
2104 1800 836 281
AgeI A'CCGG_T
Cuts at: 1999 1999
Size: 5021
AhdI GACnn_n'nnGTC
Cuts at: 1577 1577
Size: 5021
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 851 1727 851
Size: 876 4145
AlwNI CAG_nnn'CTG
Cuts at: 1603 2326 3764 1603
Size: 723 1438 2860
Fragments arranged by size:
2860 1438 723
ApaI G_GGCC'C
Cuts at: 215 215
Size: 5021
ApaLI G'TGCA_C
Cuts at: 3859 3859
Size: 5021
ApoI r'AATT_y
Cuts at: 324 673 696 887 943 2025 4374 4630 324
Size: 349 23 191 56 1082 2349 256 715
Fragments arranged by size:
2349 1082 715 349 256 191 56 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 5021
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5021
AvaI C'yCGr_G
Cuts at: 1897 2041 1897
Size: 144 4877
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 969 1002 969
Size: 33 4988
BamHI G'GATC_C
Cuts at: 2046 2046
Size: 5021
BanI G'GyrC_C
Cuts at: 1429 2037 2451 2699 2734 1429
Size: 608 414 248 35 3716
Fragments arranged by size:
3716 608 414 248 35
BanII G_rGCy'C
Cuts at: 215 875 906 2035 3065 215
Size: 660 31 1129 1030 2171
Fragments arranged by size:
2171 1129 1030 660 31
BbeI G_GCGC'C
Cuts at: 2455 2703 2455
Size: 248 4773
BbsI GAAGACnn'nnnn_
Cuts at: 713 713
Size: 5021
BbvCI CC'TCA_GC
Cuts at: 1667 1667
Size: 5021
BciVI GTATCCnnnnn_n'
Cuts at: 2919 3975 5021 2919
Size: 1056 1046 2919
Fragments arranged by size:
2919 1056 1046
BclI T'GATC_A
Cuts at: 245 2541 245
Size: 2296 2725
BglII A'GATC_T
Cuts at: 2536 4573 2536
Size: 2037 2984
BlpI GC'TnA_GC
Cuts at: 1211 2133 1211
Size: 922 4099
Bme1580I G_kGCm'C
Cuts at: 123 215 2646 2739 3863 123
Size: 92 2431 93 1124 1281
Fragments arranged by size:
2431 1281 1124 93 92
BmgBI CAC'GTC
Cuts at: 171 1585 171
Size: 1414 3607
BmrI ACTGGGnnnn_n'
Cuts at: 110 2233 2314 2649 4793 110
Size: 2123 81 335 2144 338
Fragments arranged by size:
2144 2123 338 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3388 3388
Size: 5021
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1889 1889
Size: 5021
Bpu10I CC'TnA_GC
Cuts at: 1424 1667 1424
Size: 243 4778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1757 2197 3076 3790 4088 1757
Size: 440 879 714 298 2690
Fragments arranged by size:
2690 879 714 440 298
BsaI GGTCTCn'nnnn_
Cuts at: 371 1500 4725 371
Size: 1129 3225 667
Fragments arranged by size:
3225 1129 667
BsaAI yAC'GTr
Cuts at: 150 2374 150
Size: 2224 2797
BsaBI GATnn'nnATC
Cuts at: 6 2535 4572 6
Size: 2529 2037 455
Fragments arranged by size:
2529 2037 455
BsaHI Gr'CG_yC
Cuts at: 2452 2700 3402 4898 2452
Size: 248 702 1496 2575
Fragments arranged by size:
2575 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1037 1999 2210 2731 3409 3820 3967 4241 1037
Size: 962 211 521 678 411 147 274 1817
Fragments arranged by size:
1817 962 678 521 411 274 211 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1119 1760 1119
Size: 641 4380
BseYI C'CCAG_C
Cuts at: 124 1473 2446 3204 3869 4277 124
Size: 1349 973 758 665 408 868
Fragments arranged by size:
1349 973 868 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 209 2563 2609 3839 104
Size: 105 2354 46 1230 1286
Fragments arranged by size:
2354 1286 1230 105 46
BsiHKAI G_wGCw'C
Cuts at: 875 906 2035 2813 3003 3863 875
Size: 31 1129 778 190 860 2033
Fragments arranged by size:
2033 1129 860 778 190 31
BsmI GAATG_Cn'
Cuts at: 593 593
Size: 5021
BsmBI CGTCTCn'nnnn_
Cuts at: 538 1039 538
Size: 501 4520
Bsp1286I G_dGCh'C
Cuts at: 123 215 875 906 2035 2646 2739 2813 3003
Size: 92 660 31 1129 611 93 74 190
Cuts at: 3003 3065 3863 123
Size: 62 798 1281
Fragments arranged by size:
1281 1129 798 660 611 190 93 92
74 62 31
BspEI T'CCGG_A
Cuts at: 1037 1037
Size: 5021
BspHI T'CATG_A
Cuts at: 3453 3453
Size: 5021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2071 2285 2587 2968 2071
Size: 214 302 381 4124
Fragments arranged by size:
4124 381 302 214
BsrBI CCG'CTC
Cuts at: 50 257 3313 3367 4851 5016 50
Size: 207 3056 54 1484 165 55
Fragments arranged by size:
3056 1484 207 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2933 2933
Size: 5021
BsrFI r'CCGG_y
Cuts at: 291 1999 3019 3200 291
Size: 1708 1020 181 2112
Fragments arranged by size:
2112 1708 1020 181
BsrGI T'GTAC_A
Cuts at: 534 1722 534
Size: 1188 3833
BssSI C'ACGA_G
Cuts at: 1529 3292 4000 1529
Size: 1763 708 2550
Fragments arranged by size:
2550 1763 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1603 2398 1603
Size: 795 4226
BstBI TT'CG_AA
Cuts at: 3382 3382
Size: 5021
BstYI r'GATC_y
Cuts at: 1 732 2046 2513 2536 2871 3117 3423 3435
Size: 731 1314 467 23 335 246 306 12
Cuts at: 3435 3521 4573 1
Size: 86 1052 449
Fragments arranged by size:
1314 1052 731 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 134 134
Size: 5021
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 196 2971 3152 3315 196
Size: 2775 181 163 1902
Fragments arranged by size:
2775 1902 181 163
BtsI GCAGTG_nn'
Cuts at: 2264 2264
Size: 5021
DraI TTT'AAA
Cuts at: 2020 2020
Size: 5021
DraIII CAC_nnn'GTG
Cuts at: 1549 1549
Size: 5021
DrdI GACnn_nn'nnGTC
Cuts at: 2727 4071 2727
Size: 1344 3677
EaeI y'GGCC_r
Cuts at: 206 1181 2606 2780 3171 3198 4543 206
Size: 975 1425 174 391 27 1345 684
Fragments arranged by size:
1425 1345 975 684 391 174 27
EagI C'GGCC_G
Cuts at: 206 2606 206
Size: 2400 2621
EarI CTCTTCn'nnn_
Cuts at: 1217 3044 3254 1217
Size: 1827 210 2984
Fragments arranged by size:
2984 1827 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 310 1459 3229 3971 4117 4831 310
Size: 1149 1770 742 146 714 500
Fragments arranged by size:
1770 1149 742 714 500 146
EcoICRI GAG'CTC
Cuts at: 873 904 2033 873
Size: 31 1129 3861
Fragments arranged by size:
3861 1129 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1307 1889 2846 3278 3631 4293 1307
Size: 582 957 432 353 662 2035
Fragments arranged by size:
2035 957 662 582 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1322 1322
Size: 5021
EcoO109I rG'GnC_Cy
Cuts at: 156 2160 156
Size: 2004 3017
EcoRI G'AATT_C
Cuts at: 673 2025 673
Size: 1352 3669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 670 753 3040 670
Size: 83 2287 2651
Fragments arranged by size:
2651 2287 83
FspI TGC'GCA
Cuts at: 2802 2802
Size: 5021
HaeII r_GCGC'y
Cuts at: 726 1230 2455 2703 3933 4704 726
Size: 504 1225 248 1230 771 1043
Fragments arranged by size:
1230 1225 1043 771 504 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1563 1595 2863 2893 2895 2925 1563
Size: 32 1268 30 2 30 3659
Fragments arranged by size:
3659 1268 32 30 30 2
HincII GTy'rAC
Cuts at: 1541 1989 2060 1541
Size: 448 71 4502
Fragments arranged by size:
4502 448 71
HindIII A'AGCT_T
Cuts at: 816 816
Size: 5021
HpaI GTT'AAC
Cuts at: 1989 1989
Size: 5021
KasI G'GCGC_C
Cuts at: 2451 2699 2451
Size: 248 4773
KpnI G_GTAC'C
Cuts at: 2041 2041
Size: 5021
MluI A'CGCG_T
Cuts at: 269 4454 269
Size: 4185 836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 597 1844 2032 3780 3964 597
Size: 1247 188 1748 184 1654
Fragments arranged by size:
1748 1654 1247 188 184
MscI TGG'CCA
Cuts at: 1183 2782 1183
Size: 1599 3422
MslI CAynn'nnrTG
Cuts at: 833 1679 1855 3010 3137 833
Size: 846 176 1155 127 2717
Fragments arranged by size:
2717 1155 846 176 127
MspA1I CmG'CkG
Cuts at: 559 1477 1594 1603 2132 2446 2806 3590 3835
Size: 918 117 9 529 314 360 784 245
Cuts at: 3835 559
Size: 1745
Fragments arranged by size:
1745 918 784 529 360 314 245 117
9
NaeI GCC'GGC
Cuts at: 293 3202 293
Size: 2909 2112
NarI GG'CG_CC
Cuts at: 2452 2700 2452
Size: 248 4773
NcoI C'CATG_G
Cuts at: 134 134
Size: 5021
NdeI CA'TA_TG
Cuts at: 1892 1892
Size: 5021
NgoMIV G'CCGG_C
Cuts at: 291 3200 291
Size: 2909 2112
NspI r_CATG'y
Cuts at: 1860 2074 2574 4177 1860
Size: 214 500 1603 2704
Fragments arranged by size:
2704 1603 500 214
PciI A'CATG_T
Cuts at: 4173 4173
Size: 5021
PmeI GTTT'AAAC
Cuts at: 2020 2020
Size: 5021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3467 3499 3467
Size: 32 4989
PpuMI rG'GwC_Cy
Cuts at: 156 156
Size: 5021
PsiI TTA'TAA
Cuts at: 337 337
Size: 5021
PspOMI G'GGCC_C
Cuts at: 211 211
Size: 5021
PspXI vC'TCGA_Gb
Cuts at: 1897 1897
Size: 5021
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1265 1297 1265
Size: 32 4989
PstI C_TGCA'G
Cuts at: 238 1593 1599 1608 1653 2068 2753 238
Size: 1355 6 9 45 415 685 2506
Fragments arranged by size:
2506 1355 685 415 45 9 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5021
PvuII CAG'CTG
Cuts at: 559 1477 1594 2446 2806 559
Size: 918 117 852 360 2774
Fragments arranged by size:
2774 918 852 360 117
RsrII CG'GwC_CG
Cuts at: 3216 3216
Size: 5021
SacI G_AGCT'C
Cuts at: 875 906 2035 875
Size: 31 1129 3861
Fragments arranged by size:
3861 1129 31
SalI G'TCGA_C
Cuts at: 2058 2058
Size: 5021
SapI GCTCTTCn'nnn_
Cuts at: 1217 3044 3254 1217
Size: 1827 210 2984
Fragments arranged by size:
2984 1827 210
SbfI CC_TGCA'GG
Cuts at: 2068 2068
Size: 5021
ScaI AGT'ACT
Cuts at: 1500 4538 1500
Size: 3038 1983
SfcI C'TryA_G
Cuts at: 33 234 1589 1595 1604 1649 1937 2064 2749
Size: 201 1355 6 9 45 288 127 685
Cuts at: 2749 3717 3908 33
Size: 968 191 1146
Fragments arranged by size:
1355 1146 968 685 288 201 191 127
45 9 6
SfoI GGC'GCC
Cuts at: 2453 2701 2453
Size: 248 4773
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5021
SgrAI Cr'CCGG_yG
Cuts at: 1999 1999
Size: 5021
SmaI CCC'GGG
Cuts at: 2043 2043
Size: 5021
SmlI C'TyrA_G
Cuts at: 727 759 934 1772 1897 2176 3091 3805 4067
Size: 32 175 838 125 279 915 714 262
Cuts at: 4067 727
Size: 1681
Fragments arranged by size:
1681 915 838 714 279 262 175 125
32
SpeI A'CTAG_T
Cuts at: 67 1069 67
Size: 1002 4019
SphI G_CATG'C
Cuts at: 2074 2574 2074
Size: 500 4521
StyI C'CwwG_G
Cuts at: 134 260 518 2155 2509 134
Size: 126 258 1637 354 2646
Fragments arranged by size:
2646 1637 354 258 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 190 1783 2604 2737 3196 3405 190
Size: 1593 821 133 459 209 1806
Fragments arranged by size:
1806 1593 821 459 209 133
TatI w'GTAC_w
Cuts at: 534 1498 1722 1944 4536 534
Size: 964 224 222 2592 1019
Fragments arranged by size:
2592 1019 964 224 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1989 2021 4538 4570 1989
Size: 32 2517 32 2440
Fragments arranged by size:
2517 2440 32 32
Tth111I GACn'n_nGTC
Cuts at: 1406 2818 1406
Size: 1412 3609
XbaI T'CTAG_A
Cuts at: 2052 2052
Size: 5021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 1412 2516 132
Size: 1280 1104 2637
Fragments arranged by size:
2637 1280 1104
XhoI C'TCGA_G
Cuts at: 1897 1897
Size: 5021
XmaI C'CCGG_G
Cuts at: 2041 2041
Size: 5021
XmnI GAAnn'nnTTC
Cuts at: 1886 1886
Size: 5021
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PpuMI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AloI AscI AvrII BcgI
BfrBI BglI BmtI BsaXI BsgI BsiWI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI ZraI