Restriction map for
pF1KB5694
(Circular) (Six-base) MAPSORT of: pF1KB5694.seq Check: 8956 from: 1 to: 5051
pF1KB5694 5051 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 1145 1145
Size: 5051
AccI GT'mk_AC
Cuts at: 287 503 2089 287
Size: 216 1586 3249
Fragments arranged by size:
3249 1586 216
Acc65I G'GTAC_C
Cuts at: 605 2067 605
Size: 1462 3589
AclI AA'CG_TT
Cuts at: 4891 4891
Size: 5051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 670 751 913 936 1267 2876 3308 3661
Size: 405 81 162 23 331 1609 432 353
Cuts at: 3661 4323 265
Size: 662 993
Fragments arranged by size:
1609 993 662 432 405 353 331 162
81 23
AflIII A'CryG_T
Cuts at: 2403 4203 4484 2403
Size: 1800 281 2970
Fragments arranged by size:
2970 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1213 1245 1213
Size: 32 5019
AlwNI CAG_nnn'CTG
Cuts at: 1074 1165 1239 1263 2356 3794 1074
Size: 91 74 24 1093 1438 2331
Fragments arranged by size:
2331 1438 1093 91 74 24
ApaLI G'TGCA_C
Cuts at: 3889 3889
Size: 5051
ApoI r'AATT_y
Cuts at: 2055 4404 4660 2055
Size: 2349 256 2446
Fragments arranged by size:
2446 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5051
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
AvaI C'yCGr_G
Cuts at: 1225 2071 1225
Size: 846 4205
BamHI G'GATC_C
Cuts at: 2076 2076
Size: 5051
BanI G'GyrC_C
Cuts at: 605 1475 2067 2481 2729 2764 605
Size: 870 592 414 248 35 2892
Fragments arranged by size:
2892 870 592 414 248 35
BanII G_rGCy'C
Cuts at: 782 881 2007 2065 3095 782
Size: 99 1126 58 1030 2738
Fragments arranged by size:
2738 1126 1030 99 58
BbeI G_GCGC'C
Cuts at: 2485 2733 2485
Size: 248 4803
BbsI GAAGACnn'nnnn_
Cuts at: 758 758
Size: 5051
BbvCI CC'TCA_GC
Cuts at: 277 1702 277
Size: 1425 3626
BciVI GTATCCnnnnn_n'
Cuts at: 2949 4005 5051 2949
Size: 1056 1046 2949
Fragments arranged by size:
2949 1056 1046
BclI T'GATC_A
Cuts at: 2571 2571
Size: 5051
BglI GCCn_nnn'nGGC
Cuts at: 1878 1878
Size: 5051
BglII A'GATC_T
Cuts at: 2566 4603 2566
Size: 2037 3014
BlpI GC'TnA_GC
Cuts at: 191 382 2163 191
Size: 191 1781 3079
Fragments arranged by size:
3079 1781 191
Bme1580I G_kGCm'C
Cuts at: 182 1343 1478 2676 2769 3893 182
Size: 1161 135 1198 93 1124 1340
Fragments arranged by size:
1340 1198 1161 1124 135 93
BmrI ACTGGGnnnn_n'
Cuts at: 255 759 791 2263 2344 2679 4823 255
Size: 504 32 1472 81 335 2144 483
Fragments arranged by size:
2144 1472 504 483 335 81 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3418 3418
Size: 5051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1048 1669 373
Size: 675 621 3755
Fragments arranged by size:
3755 675 621
Bpu10I CC'TnA_GC
Cuts at: 206 277 1549 1702 1884 206
Size: 71 1272 153 182 3373
Fragments arranged by size:
3373 1272 182 153 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 602 2227 3106 3820 4118 404
Size: 198 1625 879 714 298 1337
Fragments arranged by size:
1625 1337 879 714 298 198
BsaI GGTCTCn'nnnn_
Cuts at: 2006 4755 2006
Size: 2749 2302
BsaAI yAC'GTr
Cuts at: 2404 2404
Size: 5051
BsaBI GATnn'nnATC
Cuts at: 6 2565 4602 6
Size: 2559 2037 455
Fragments arranged by size:
2559 2037 455
BsaHI Gr'CG_yC
Cuts at: 1142 1333 2482 2730 3432 4928 1142
Size: 191 1149 248 702 1496 1265
Fragments arranged by size:
1496 1265 1149 702 248 191
BsaWI w'CCGG_w
Cuts at: 513 2240 2761 3439 3850 3997 4271 513
Size: 1727 521 678 411 147 274 1293
Fragments arranged by size:
1727 1293 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1729 1759 1729
Size: 30 5021
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 532 589 670 532
Size: 57 81 4913
Fragments arranged by size:
4913 81 57
BseYI C'CCAG_C
Cuts at: 973 1342 1830 2476 3234 3899 4307 973
Size: 369 488 646 758 665 408 1717
Fragments arranged by size:
1717 758 665 646 488 408 369
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1896 1896
Size: 5051
BsiEI CG_ry'CG
Cuts at: 104 2593 2639 3869 104
Size: 2489 46 1230 1286
Fragments arranged by size:
2489 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 782 1555 2065 2843 3033 3893 782
Size: 773 510 778 190 860 1940
Fragments arranged by size:
1940 860 778 773 510 190
BsmI GAATG_Cn'
Cuts at: 893 1408 1606 893
Size: 515 198 4338
Fragments arranged by size:
4338 515 198
BsmBI CGTCTCn'nnnn_
Cuts at: 1919 1919
Size: 5051
Bsp1286I G_dGCh'C
Cuts at: 182 782 881 1343 1478 1555 2007 2065 2676
Size: 600 99 462 135 77 452 58 611
Cuts at: 2676 2769 2843 3033 3095 3893 182
Size: 93 74 190 62 798 1340
Fragments arranged by size:
1340 798 611 600 462 452 190 135
99 93 77 74 62 58
BspEI T'CCGG_A
Cuts at: 513 513
Size: 5051
BspHI T'CATG_A
Cuts at: 3483 3483
Size: 5051
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2101 2315 2617 2998 2101
Size: 214 302 381 4154
Fragments arranged by size:
4154 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3343 3397 4881 5046 50
Size: 3293 54 1484 165 55
Fragments arranged by size:
3293 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2963 2963
Size: 5051
BsrFI r'CCGG_y
Cuts at: 641 788 3049 3230 641
Size: 147 2261 181 2462
Fragments arranged by size:
2462 2261 181 147
BsrGI T'GTAC_A
Cuts at: 710 710
Size: 5051
BssSI C'ACGA_G
Cuts at: 1567 3322 4030 1567
Size: 1755 708 2588
Fragments arranged by size:
2588 1755 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2428 2428
Size: 5051
BstBI TT'CG_AA
Cuts at: 3412 3412
Size: 5051
BstXI CCAn_nnnn'nTGG
Cuts at: 1620 1620
Size: 5051
BstYI r'GATC_y
Cuts at: 1 1533 2076 2543 2566 2901 3147 3453 3465
Size: 1532 543 467 23 335 246 306 12
Cuts at: 3465 3551 4603 1
Size: 86 1052 449
Fragments arranged by size:
1532 1052 543 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 572 572
Size: 5051
BtgI C'CryG_G
Cuts at: 107 881 107
Size: 774 4277
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3001 3182 3345 3001
Size: 181 163 4707
Fragments arranged by size:
4707 181 163
BtsI GCAGTG_nn'
Cuts at: 1712 2294 1712
Size: 582 4469
ClaI AT'CG_AT
Cuts at: 2033 2033
Size: 5051
DraI TTT'AAA
Cuts at: 2050 2050
Size: 5051
DrdI GACnn_nn'nnGTC
Cuts at: 696 1015 2757 4101 696
Size: 319 1742 1344 1646
Fragments arranged by size:
1742 1646 1344 319
EaeI y'GGCC_r
Cuts at: 639 1382 2636 2810 3201 3228 4573 639
Size: 743 1254 174 391 27 1345 1117
Fragments arranged by size:
1345 1254 1117 743 391 174 27
EagI C'GGCC_G
Cuts at: 2636 2636
Size: 5051
EarI CTCTTCn'nnn_
Cuts at: 989 1241 1648 1958 3074 3284 989
Size: 252 407 310 1116 210 2756
Fragments arranged by size:
2756 1116 407 310 252 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3259 4001 4147 4861 3259
Size: 742 146 714 3449
Fragments arranged by size:
3449 742 714 146
EcoICRI GAG'CTC
Cuts at: 780 2063 780
Size: 1283 3768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 373 670 751 913 936 1048 1267 1669
Size: 108 297 81 162 23 112 219 402
Cuts at: 1669 2876 3308 3661 4323 265
Size: 1207 432 353 662 993
Fragments arranged by size:
1207 993 662 432 402 353 297 219
162 112 108 81 23
EcoNI CCTnn'n_nnAGG
Cuts at: 1978 1978
Size: 5051
EcoO109I rG'GnC_Cy
Cuts at: 1017 1071 1221 1273 2190 1017
Size: 54 150 52 917 3878
Fragments arranged by size:
3878 917 150 54 52
EcoRI G'AATT_C
Cuts at: 2055 2055
Size: 5051
EcoRV GAT'ATC
Cuts at: 1094 1094
Size: 5051
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3070 3070
Size: 5051
FseI GG_CCGG'CC
Cuts at: 645 645
Size: 5051
FspI TGC'GCA
Cuts at: 2832 2832
Size: 5051
HaeII r_GCGC'y
Cuts at: 468 1059 1838 1884 2485 2733 3963 4734 468
Size: 591 779 46 601 248 1230 771 785
Fragments arranged by size:
1230 785 779 771 601 591 248 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2893 2923 2925 2955 2893
Size: 30 2 30 4989
Fragments arranged by size:
4989 30 30 2
HincII GTy'rAC
Cuts at: 288 537 1377 1506 2090 288
Size: 249 840 129 584 3249
Fragments arranged by size:
3249 840 584 249 129
KasI G'GCGC_C
Cuts at: 2481 2729 2481
Size: 248 4803
KpnI G_GTAC'C
Cuts at: 609 2071 609
Size: 1462 3589
MluI A'CGCG_T
Cuts at: 4484 4484
Size: 5051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1565 1637 1709 3810 3994 1565
Size: 72 72 2101 184 2622
Fragments arranged by size:
2622 2101 184 72 72
MscI TGG'CCA
Cuts at: 1384 2812 1384
Size: 1428 3623
MslI CAynn'nnrTG
Cuts at: 553 3040 3167 553
Size: 2487 127 2437
Fragments arranged by size:
2487 2437 127
MspA1I CmG'CkG
Cuts at: 378 741 1371 1991 2162 2476 2836 3620 3865
Size: 363 630 620 171 314 360 784 245
Cuts at: 3865 378
Size: 1564
Fragments arranged by size:
1564 784 630 620 363 360 314 245
171
NaeI GCC'GGC
Cuts at: 643 3232 643
Size: 2589 2462
NarI GG'CG_CC
Cuts at: 2482 2730 2482
Size: 248 4803
NcoI C'CATG_G
Cuts at: 107 107
Size: 5051
NgoMIV G'CCGG_C
Cuts at: 641 3230 641
Size: 2589 2462
NspI r_CATG'y
Cuts at: 2047 2104 2604 4207 2047
Size: 57 500 1603 2891
Fragments arranged by size:
2891 1603 500 57
PasI CC'CwG_GG
Cuts at: 543 543
Size: 5051
PciI A'CATG_T
Cuts at: 4203 4203
Size: 5051
PflMI CCAn_nnn'nTGG
Cuts at: 693 1263 1496 693
Size: 570 233 4248
Fragments arranged by size:
4248 570 233
PfoI T'CCnGG_A
Cuts at: 389 1495 1646 2007 389
Size: 1106 151 361 3433
Fragments arranged by size:
3433 1106 361 151
PmeI GTTT'AAAC
Cuts at: 2050 2050
Size: 5051
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 5019
PpuMI rG'GwC_Cy
Cuts at: 1017 1221 1017
Size: 204 4847
PshAI GACnn'nnGTC
Cuts at: 421 421
Size: 5051
PspXI vC'TCGA_Gb
Cuts at: 1225 1225
Size: 5051
PstI C_TGCA'G
Cuts at: 1982 2098 2783 1982
Size: 116 685 4250
Fragments arranged by size:
4250 685 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5051
PvuII CAG'CTG
Cuts at: 741 1371 2476 2836 741
Size: 630 1105 360 2956
Fragments arranged by size:
2956 1105 630 360
RsrII CG'GwC_CG
Cuts at: 3246 3246
Size: 5051
SacI G_AGCT'C
Cuts at: 782 2065 782
Size: 1283 3768
SalI G'TCGA_C
Cuts at: 286 2088 286
Size: 1802 3249
SapI GCTCTTCn'nnn_
Cuts at: 3074 3284 3074
Size: 210 4841
SbfI CC_TGCA'GG
Cuts at: 1982 2098 1982
Size: 116 4935
ScaI AGT'ACT
Cuts at: 4568 4568
Size: 5051
SfcI C'TryA_G
Cuts at: 33 1978 2094 2779 3747 3938 33
Size: 1945 116 685 968 191 1146
Fragments arranged by size:
1945 1146 968 685 191 116
SfoI GGC'GCC
Cuts at: 2483 2731 2483
Size: 248 4803
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
SmaI CCC'GGG
Cuts at: 2073 2073
Size: 5051
SmlI C'TyrA_G
Cuts at: 419 617 1225 2206 3121 3835 4097 419
Size: 198 608 981 915 714 262 1373
Fragments arranged by size:
1373 981 915 714 608 262 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5051
SphI G_CATG'C
Cuts at: 2104 2604 2104
Size: 500 4551
StyI C'CwwG_G
Cuts at: 107 267 1399 2185 2539 107
Size: 160 1132 786 354 2619
Fragments arranged by size:
2619 1132 786 354 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 461 1419 2634 2767 3226 3435 461
Size: 958 1215 133 459 209 2077
Fragments arranged by size:
2077 1215 958 459 209 133
TatI w'GTAC_w
Cuts at: 710 4566 710
Size: 3856 1195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 904 936 1529 1561 1704 1736 4568 4600 904
Size: 32 593 32 143 32 2832 32 1355
Fragments arranged by size:
2832 1355 593 143 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2848 2848
Size: 5051
XbaI T'CTAG_A
Cuts at: 2082 2082
Size: 5051
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 358 2546 358
Size: 2188 2863
XhoI C'TCGA_G
Cuts at: 1225 1225
Size: 5051
XmaI C'CCGG_G
Cuts at: 2071 2071
Size: 5051
ZraI GAC'GTC
Cuts at: 1143 1143
Size: 5051
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI
BssHII BstEII BstZ17I CspCI DraIII FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI