Restriction map for
pF1KB5688
(Circular) (Six-base) MAPSORT of: pF1KB5688.seq Check: 7089 from: 1 to: 5198
pF1KB5688 5198 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 1697 1697
Size: 5198
AccI GT'mk_AC
Cuts at: 683 2236 683
Size: 1553 3645
Acc65I G'GTAC_C
Cuts at: 2214 2214
Size: 5198
AclI AA'CG_TT
Cuts at: 5038 5038
Size: 5198
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 854 1618 1950 3023 3455 3808 4470 355
Size: 499 764 332 1073 432 353 662 1083
Fragments arranged by size:
1083 1073 764 662 499 432 353 332
AflIII A'CryG_T
Cuts at: 2550 4350 4631 2550
Size: 1800 281 3117
Fragments arranged by size:
3117 1800 281
AleI CACnn'nnGTG
Cuts at: 412 412
Size: 5198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 169 169
Size: 5198
AlwNI CAG_nnn'CTG
Cuts at: 1227 1293 2503 3941 1227
Size: 66 1210 1438 2484
Fragments arranged by size:
2484 1438 1210 66
ApaI G_GGCC'C
Cuts at: 1951 1951
Size: 5198
ApaLI G'TGCA_C
Cuts at: 4036 4036
Size: 5198
ApoI r'AATT_y
Cuts at: 517 823 1607 2202 4551 4807 517
Size: 306 784 595 2349 256 908
Fragments arranged by size:
2349 908 784 595 306 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5198
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5198
AvaI C'yCGr_G
Cuts at: 1313 1924 2218 1313
Size: 611 294 4293
Fragments arranged by size:
4293 611 294
BamHI G'GATC_C
Cuts at: 2223 2223
Size: 5198
BanI G'GyrC_C
Cuts at: 180 334 452 991 1139 1813 2068 2214 2628
Size: 154 118 539 148 674 255 146 414
Cuts at: 2628 2876 2911 180
Size: 248 35 2467
Fragments arranged by size:
2467 674 539 414 255 248 154 148
146 118 35
BanII G_rGCy'C
Cuts at: 176 1631 1708 1951 2212 3242 176
Size: 1455 77 243 261 1030 2132
Fragments arranged by size:
2132 1455 1030 261 243 77
BbeI G_GCGC'C
Cuts at: 184 2632 2880 184
Size: 2448 248 2502
Fragments arranged by size:
2502 2448 248
BbsI GAAGACnn'nnnn_
Cuts at: 806 854 806
Size: 48 5150
BbvCI CC'TCA_GC
Cuts at: 2050 2050
Size: 5198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 199 233 1063 1097 199
Size: 34 830 34 4300
Fragments arranged by size:
4300 830 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 401 3096 4152 5198 401
Size: 2695 1056 1046 401
Fragments arranged by size:
2695 1056 1046 401
BclI T'GATC_A
Cuts at: 2718 2718
Size: 5198
BfrBI ATG'CAT
Cuts at: 291 291
Size: 5198
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5198
BglII A'GATC_T
Cuts at: 840 2713 4750 840
Size: 1873 2037 1288
Fragments arranged by size:
2037 1873 1288
BlpI GC'TnA_GC
Cuts at: 251 2310 251
Size: 2059 3139
Bme1580I G_kGCm'C
Cuts at: 1951 2823 2916 4040 1951
Size: 872 93 1124 3109
Fragments arranged by size:
3109 1124 872 93
BmrI ACTGGGnnnn_n'
Cuts at: 317 2410 2491 2826 4970 317
Size: 2093 81 335 2144 545
Fragments arranged by size:
2144 2093 545 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1241 3565 1241
Size: 2324 2874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 1375 1393 1802 2197 1150
Size: 225 18 409 395 4151
Fragments arranged by size:
4151 409 395 225 18
Bpu10I CC'TnA_GC
Cuts at: 1118 2050 1118
Size: 932 4266
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2374 3253 3967 4265 2374
Size: 879 714 298 3307
Fragments arranged by size:
3307 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1069 1294 1675 4902 1069
Size: 225 381 3227 1365
Fragments arranged by size:
3227 1365 381 225
BsaAI yAC'GTr
Cuts at: 1542 2551 1542
Size: 1009 4189
BsaBI GATnn'nnATC
Cuts at: 6 2712 4749 6
Size: 2706 2037 455
Fragments arranged by size:
2706 2037 455
BsaHI Gr'CG_yC
Cuts at: 181 1694 2629 2877 3579 5075 181
Size: 1513 935 248 702 1496 304
Fragments arranged by size:
1513 1496 935 702 304 248
BsaWI w'CCGG_w
Cuts at: 2387 2908 3586 3997 4144 4418 2387
Size: 521 678 411 147 274 3167
Fragments arranged by size:
3167 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 976 1031 1726 1735 976
Size: 55 695 9 4439
Fragments arranged by size:
4439 695 55 9
BseYI C'CCAG_C
Cuts at: 1755 2623 3381 4046 4454 1755
Size: 868 758 665 408 2499
Fragments arranged by size:
2499 868 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1667 1667
Size: 5198
BsiEI CG_ry'CG
Cuts at: 104 131 2740 2786 4016 104
Size: 27 2609 46 1230 1286
Fragments arranged by size:
2609 1286 1230 46 27
BsiHKAI G_wGCw'C
Cuts at: 1631 1708 1986 2212 2990 3180 4040 1631
Size: 77 278 226 778 190 860 2789
Fragments arranged by size:
2789 860 778 278 226 190 77
BsmI GAATG_Cn'
Cuts at: 1403 1403
Size: 5198
Bsp1286I G_dGCh'C
Cuts at: 176 1631 1708 1951 1986 2212 2823 2916 2990
Size: 1455 77 243 35 226 611 93 74
Cuts at: 2990 3180 3242 4040 176
Size: 190 62 798 1334
Fragments arranged by size:
1455 1334 798 611 243 226 190 93
77 74 62 35
BspHI T'CATG_A
Cuts at: 1017 3630 1017
Size: 2613 2585
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2248 2462 2764 3145 2248
Size: 214 302 381 4301
Fragments arranged by size:
4301 381 302 214
BsrBI CCG'CTC
Cuts at: 50 573 1935 3490 3544 5028 5193 50
Size: 523 1362 1555 54 1484 165 55
Fragments arranged by size:
1555 1484 1362 523 165 55 54
BsrDI GCAATG_nn'
Cuts at: 272 478 2015 3110 272
Size: 206 1537 1095 2360
Fragments arranged by size:
2360 1537 1095 206
BsrFI r'CCGG_y
Cuts at: 199 3196 3377 199
Size: 2997 181 2020
Fragments arranged by size:
2997 2020 181
BssSI C'ACGA_G
Cuts at: 508 3469 4177 508
Size: 2961 708 1529
Fragments arranged by size:
2961 1529 708
BstAPI GCAn_nnn'nTGC
Cuts at: 226 2575 226
Size: 2349 2849
BstBI TT'CG_AA
Cuts at: 3559 3559
Size: 5198
BstXI CCAn_nnnn'nTGG
Cuts at: 694 694
Size: 5198
BstYI r'GATC_y
Cuts at: 1 736 840 1724 1777 2223 2690 2713 3048
Size: 735 104 884 53 446 467 23 335
Cuts at: 3048 3294 3600 3612 3698 4750 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 884 735 467 449 446 335 306
246 104 86 53 23 12
BtgI C'CryG_G
Cuts at: 107 125 140 693 1247 1390 1943 2021 2079
Size: 18 15 553 554 143 553 78 58
Cuts at: 2079 2145 107
Size: 66 3160
Fragments arranged by size:
3160 554 553 553 143 78 66 58
18 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1384 1989 3148 3329 3492 1384
Size: 605 1159 181 163 3090
Fragments arranged by size:
3090 1159 605 181 163
BtsI GCAGTG_nn'
Cuts at: 1664 1951 1986 2015 2441 1664
Size: 287 35 29 426 4421
Fragments arranged by size:
4421 426 287 35 29
DraI TTT'AAA
Cuts at: 2197 2197
Size: 5198
DrdI GACnn_nn'nnGTC
Cuts at: 2904 4248 2904
Size: 1344 3854
EaeI y'GGCC_r
Cuts at: 113 128 197 2012 2783 2957 3348 3375 4720
Size: 15 69 1815 771 174 391 27 1345
Cuts at: 4720 113
Size: 591
Fragments arranged by size:
1815 1345 771 591 391 174 69 27
15
EagI C'GGCC_G
Cuts at: 128 2783 128
Size: 2655 2543
EarI CTCTTCn'nnn_
Cuts at: 746 752 764 770 776 788 1172 1814 1925
Size: 6 12 6 6 12 384 642 111
Cuts at: 1925 2050 2111 3221 3431 746
Size: 125 61 1110 210 2513
Fragments arranged by size:
2513 1110 642 384 210 125 111 61
12 12 6 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3406 4148 4294 5008 3406
Size: 742 146 714 3596
Fragments arranged by size:
3596 742 714 146
EcoICRI GAG'CTC
Cuts at: 1629 1706 2210 1629
Size: 77 504 4617
Fragments arranged by size:
4617 504 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 854 1150 1375 1393 1618 1802 1950 2197
Size: 499 296 225 18 225 184 148 247
Cuts at: 2197 3023 3455 3808 4470 355
Size: 826 432 353 662 1083
Fragments arranged by size:
1083 826 662 499 432 353 296 247
225 225 184 148 18
EcoO109I rG'GnC_Cy
Cuts at: 2337 2337
Size: 5198
EcoRI G'AATT_C
Cuts at: 2202 2202
Size: 5198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 363 385 501 3217 363
Size: 22 116 2716 2344
Fragments arranged by size:
2716 2344 116 22
FspI TGC'GCA
Cuts at: 2979 2979
Size: 5198
HaeII r_GCGC'y
Cuts at: 184 196 2632 2880 4110 4881 184
Size: 12 2436 248 1230 771 501
Fragments arranged by size:
2436 1230 771 501 248 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1518 1550 1924 1956 3040 3070 3072 3102 1518
Size: 32 374 32 1084 30 2 30 3614
Fragments arranged by size:
3614 1084 374 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1491 2237 1491
Size: 746 4452
HindIII A'AGCT_T
Cuts at: 1256 1256
Size: 5198
KasI G'GCGC_C
Cuts at: 180 2628 2876 180
Size: 2448 248 2502
Fragments arranged by size:
2502 2448 248
KpnI G_GTAC'C
Cuts at: 2218 2218
Size: 5198
MluI A'CGCG_T
Cuts at: 4631 4631
Size: 5198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 352 1060 1324 1896 3957 4141 352
Size: 708 264 572 2061 184 1409
Fragments arranged by size:
2061 1409 708 572 264 184
MscI TGG'CCA
Cuts at: 2014 2959 2014
Size: 945 4253
MslI CAynn'nnrTG
Cuts at: 412 2008 2150 3187 3314 412
Size: 1596 142 1037 127 2296
Fragments arranged by size:
2296 1596 1037 142 127
MspA1I CmG'CkG
Cuts at: 127 432 865 1128 1293 2309 2623 2983 3767
Size: 305 433 263 165 1016 314 360 784
Cuts at: 3767 4012 127
Size: 245 1313
Fragments arranged by size:
1313 1016 784 433 360 314 305 263
245 165
NaeI GCC'GGC
Cuts at: 201 3379 201
Size: 3178 2020
NarI GG'CG_CC
Cuts at: 181 2629 2877 181
Size: 2448 248 2502
Fragments arranged by size:
2502 2448 248
NcoI C'CATG_G
Cuts at: 107 693 1247 2145 107
Size: 586 554 898 3160
Fragments arranged by size:
3160 898 586 554
NgoMIV G'CCGG_C
Cuts at: 199 3377 199
Size: 3178 2020
NotI GC'GGCC_GC
Cuts at: 128 128
Size: 5198
NsiI A_TGCA'T
Cuts at: 293 293
Size: 5198
NspI r_CATG'y
Cuts at: 295 629 666 1102 1480 2251 2751 4354 295
Size: 334 37 436 378 771 500 1603 1139
Fragments arranged by size:
1603 1139 771 500 436 378 334 37
PciI A'CATG_T
Cuts at: 4350 4350
Size: 5198
PflMI CCAn_nnn'nTGG
Cuts at: 1248 1248
Size: 5198
PmeI GTTT'AAAC
Cuts at: 2197 2197
Size: 5198
PmlI CAC'GTG
Cuts at: 1542 1542
Size: 5198
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 3644
Size: 32 5166
PshAI GACnn'nnGTC
Cuts at: 1340 1967 1340
Size: 627 4571
PsiI TTA'TAA
Cuts at: 815 815
Size: 5198
PspOMI G'GGCC_C
Cuts at: 1947 1947
Size: 5198
PspXI vC'TCGA_Gb
Cuts at: 1924 1924
Size: 5198
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2149 2181 2149
Size: 32 5166
PstI C_TGCA'G
Cuts at: 1086 2245 2930 1086
Size: 1159 685 3354
Fragments arranged by size:
3354 1159 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5198
PvuII CAG'CTG
Cuts at: 865 1128 1293 2623 2983 865
Size: 263 165 1330 360 3080
Fragments arranged by size:
3080 1330 360 263 165
RsrII CG'GwC_CG
Cuts at: 3393 3393
Size: 5198
SacI G_AGCT'C
Cuts at: 1631 1708 2212 1631
Size: 77 504 4617
Fragments arranged by size:
4617 504 77
SacII CC_GC'GG
Cuts at: 128 128
Size: 5198
SalI G'TCGA_C
Cuts at: 2235 2235
Size: 5198
SapI GCTCTTCn'nnn_
Cuts at: 1925 2111 3221 3431 1925
Size: 186 1110 210 3692
Fragments arranged by size:
3692 1110 210 186
SbfI CC_TGCA'GG
Cuts at: 2245 2245
Size: 5198
ScaI AGT'ACT
Cuts at: 376 4715 376
Size: 4339 859
SfcI C'TryA_G
Cuts at: 33 400 1082 2241 2926 3894 4085 33
Size: 367 682 1159 685 968 191 1146
Fragments arranged by size:
1159 1146 968 685 682 367 191
SfoI GGC'GCC
Cuts at: 182 2630 2878 182
Size: 2448 248 2502
Fragments arranged by size:
2502 2448 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5198
SmaI CCC'GGG
Cuts at: 2220 2220
Size: 5198
SmlI C'TyrA_G
Cuts at: 1924 2353 3268 3982 4244 1924
Size: 429 915 714 262 2878
Fragments arranged by size:
2878 915 714 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5198
SphI G_CATG'C
Cuts at: 666 1102 2251 2751 666
Size: 436 1149 500 3113
Fragments arranged by size:
3113 1149 500 436
StuI AGG'CCT
Cuts at: 1414 1922 1414
Size: 508 4690
StyI C'CwwG_G
Cuts at: 107 188 693 1247 2145 2332 2686 107
Size: 81 505 554 898 187 354 2619
Fragments arranged by size:
2619 898 554 505 354 187 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 502 932 2781 2914 3373 3582 502
Size: 430 1849 133 459 209 2118
Fragments arranged by size:
2118 1849 459 430 209 133
TatI w'GTAC_w
Cuts at: 374 4713 374
Size: 4339 859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1769 1801 4715 4747 1769
Size: 32 2914 32 2220
Fragments arranged by size:
2914 2220 32 32
Tth111I GACn'n_nGTC
Cuts at: 2995 2995
Size: 5198
XbaI T'CTAG_A
Cuts at: 2229 2229
Size: 5198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2693 2693
Size: 5198
XhoI C'TCGA_G
Cuts at: 1924 1924
Size: 5198
XmaI C'CCGG_G
Cuts at: 2218 2218
Size: 5198
XmnI GAAnn'nnTTC
Cuts at: 365 368 365
Size: 3 5195
ZraI GAC'GTC
Cuts at: 1695 1695
Size: 5198
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NsiI
NspI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BmgBI BmtI BsaXI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NruI
PacI PasI PfoI PpuMI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI