Restriction map for
pF1KB5685
(Circular) (Six-base) MAPSORT of: pF1KB5685.seq Check: 1609 from: 1 to: 4319
pF1KB5685 4319 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 139 231 139
Size: 92 4227
AccI GT'mk_AC
Cuts at: 1028 1357 1028
Size: 329 3990
Acc65I G'GTAC_C
Cuts at: 1335 1335
Size: 4319
AclI AA'CG_TT
Cuts at: 4159 4159
Size: 4319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 505 1043 2144 2576 2929 3591 501
Size: 4 538 1101 432 353 662 1229
Fragments arranged by size:
1229 1101 662 538 432 353 4
AflIII A'CryG_T
Cuts at: 1067 1671 3471 3752 1067
Size: 604 1800 281 1634
Fragments arranged by size:
1800 1634 604 281
AlwNI CAG_nnn'CTG
Cuts at: 959 1137 1624 3062 959
Size: 178 487 1438 2216
Fragments arranged by size:
2216 1438 487 178
ApaLI G'TGCA_C
Cuts at: 178 3157 178
Size: 2979 1340
ApoI r'AATT_y
Cuts at: 1323 3672 3928 1323
Size: 2349 256 1714
Fragments arranged by size:
2349 1714 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
AvaI C'yCGr_G
Cuts at: 1222 1339 1222
Size: 117 4202
BamHI G'GATC_C
Cuts at: 1344 1344
Size: 4319
BanI G'GyrC_C
Cuts at: 909 1335 1749 1997 2032 909
Size: 426 414 248 35 3196
Fragments arranged by size:
3196 426 414 248 35
BanII G_rGCy'C
Cuts at: 422 1333 2363 422
Size: 911 1030 2378
Fragments arranged by size:
2378 1030 911
BbeI G_GCGC'C
Cuts at: 1753 2001 1753
Size: 248 4071
BbsI GAAGACnn'nnnn_
Cuts at: 604 709 604
Size: 105 4214
BbvCI CC'TCA_GC
Cuts at: 874 874
Size: 4319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4285
BciVI GTATCCnnnnn_n'
Cuts at: 2217 3273 4319 2217
Size: 1056 1046 2217
Fragments arranged by size:
2217 1056 1046
BclI T'GATC_A
Cuts at: 617 1839 617
Size: 1222 3097
BglI GCCn_nnn'nGGC
Cuts at: 208 1216 208
Size: 1008 3311
BglII A'GATC_T
Cuts at: 1834 3871 1834
Size: 2037 2282
BlpI GC'TnA_GC
Cuts at: 1431 1431
Size: 4319
Bme1580I G_kGCm'C
Cuts at: 182 776 945 1944 2037 3161 182
Size: 594 169 999 93 1124 1340
Fragments arranged by size:
1340 1124 999 594 169 93
BmgBI CAC'GTC
Cuts at: 1125 1125
Size: 4319
BmrI ACTGGGnnnn_n'
Cuts at: 600 1531 1612 1947 4091 600
Size: 931 81 335 2144 828
Fragments arranged by size:
2144 931 828 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 4319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 298 705 1036 104
Size: 194 407 331 3387
Fragments arranged by size:
3387 407 331 194
Bpu10I CC'TnA_GC
Cuts at: 874 874
Size: 4319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 2374 3088 3386 1495
Size: 879 714 298 2428
Fragments arranged by size:
2428 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4023 4023
Size: 4319
BsaAI yAC'GTr
Cuts at: 198 1672 198
Size: 1474 2845
BsaBI GATnn'nnATC
Cuts at: 6 1833 3870 6
Size: 1827 2037 455
Fragments arranged by size:
2037 1827 455
BsaHI Gr'CG_yC
Cuts at: 1750 1998 2700 4196 1750
Size: 248 702 1496 1873
Fragments arranged by size:
1873 1496 702 248
BsaWI w'CCGG_w
Cuts at: 527 1508 2029 2707 3118 3265 3539 527
Size: 981 521 678 411 147 274 1307
Fragments arranged by size:
1307 981 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 344 1173 1240 344
Size: 829 67 3423
Fragments arranged by size:
3423 829 67
BseYI C'CCAG_C
Cuts at: 338 944 1744 2502 3167 3575 338
Size: 606 800 758 665 408 1082
Fragments arranged by size:
1082 800 758 665 606 408
BsiEI CG_ry'CG
Cuts at: 104 1861 1907 3137 104
Size: 1757 46 1230 1286
Fragments arranged by size:
1757 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 182 422 535 1333 2111 2301 3161 182
Size: 240 113 798 778 190 860 1340
Fragments arranged by size:
1340 860 798 778 240 190 113
BsmI GAATG_Cn'
Cuts at: 681 681
Size: 4319
Bsp1286I G_dGCh'C
Cuts at: 182 422 535 776 945 1333 1944 2037 2111
Size: 240 113 241 169 388 611 93 74
Cuts at: 2111 2301 2363 3161 182
Size: 190 62 798 1340
Fragments arranged by size:
1340 798 611 388 241 240 190 169
113 93 74 62
BspEI T'CCGG_A
Cuts at: 527 527
Size: 4319
BspHI T'CATG_A
Cuts at: 2751 2751
Size: 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 139 231 1369 1583 1885 2266 139
Size: 92 1138 214 302 381 2192
Fragments arranged by size:
2192 1138 381 302 214 92
BsrBI CCG'CTC
Cuts at: 50 2611 2665 4149 4314 50
Size: 2561 54 1484 165 55
Fragments arranged by size:
2561 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 884 2231 884
Size: 1347 2972
BsrFI r'CCGG_y
Cuts at: 2317 2498 2317
Size: 181 4138
BsrGI T'GTAC_A
Cuts at: 410 410
Size: 4319
BssSI C'ACGA_G
Cuts at: 1095 2590 3298 1095
Size: 1495 708 2116
Fragments arranged by size:
2116 1495 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1137 1696 1137
Size: 559 3760
BstBI TT'CG_AA
Cuts at: 2680 2680
Size: 4319
BstYI r'GATC_y
Cuts at: 1 480 1344 1811 1834 2169 2415 2721 2733
Size: 479 864 467 23 335 246 306 12
Cuts at: 2733 2819 3871 1
Size: 86 1052 449
Fragments arranged by size:
1052 864 479 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1033 1033
Size: 4319
BtgI C'CryG_G
Cuts at: 430 644 861 430
Size: 214 217 3888
Fragments arranged by size:
3888 217 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2269 2450 2613 2269
Size: 181 163 3975
Fragments arranged by size:
3975 181 163
BtsI GCAGTG_nn'
Cuts at: 1562 1562
Size: 4319
DraI TTT'AAA
Cuts at: 1318 1318
Size: 4319
DrdI GACnn_nn'nnGTC
Cuts at: 2025 3369 2025
Size: 1344 2975
EaeI y'GGCC_r
Cuts at: 305 693 828 1904 2078 2469 2496 3841 305
Size: 388 135 1076 174 391 27 1345 783
Fragments arranged by size:
1345 1076 783 391 388 174 135 27
EagI C'GGCC_G
Cuts at: 1904 1904
Size: 4319
EarI CTCTTCn'nnn_
Cuts at: 745 2342 2552 745
Size: 1597 210 2512
Fragments arranged by size:
2512 1597 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2527 3269 3415 4129 2527
Size: 742 146 714 2717
Fragments arranged by size:
2717 742 714 146
EcoICRI GAG'CTC
Cuts at: 420 1331 420
Size: 911 3408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 298 501 505 705 1036 1043 2144 2576
Size: 194 203 4 200 331 7 1101 432
Cuts at: 2576 2929 3591 104
Size: 353 662 832
Fragments arranged by size:
1101 832 662 432 353 331 203 200
194 7 4
EcoO109I rG'GnC_Cy
Cuts at: 1458 1458
Size: 4319
EcoRI G'AATT_C
Cuts at: 1323 1323
Size: 4319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 726 2338 726
Size: 1612 2707
FspI TGC'GCA
Cuts at: 2100 2100
Size: 4319
HaeII r_GCGC'y
Cuts at: 1138 1753 2001 3231 4002 1138
Size: 615 248 1230 771 1455
Fragments arranged by size:
1455 1230 771 615 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 566 598 1235 1267 2161 2191 2193 2223 566
Size: 32 637 32 894 30 2 30 2662
Fragments arranged by size:
2662 894 637 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1029 1358 1029
Size: 329 3990
KasI G'GCGC_C
Cuts at: 1749 1997 1749
Size: 248 4071
KpnI G_GTAC'C
Cuts at: 1339 1339
Size: 4319
MluI A'CGCG_T
Cuts at: 3752 3752
Size: 4319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3078 3262 3078
Size: 184 4135
MscI TGG'CCA
Cuts at: 307 830 2080 307
Size: 523 1250 2546
Fragments arranged by size:
2546 1250 523
MslI CAynn'nnrTG
Cuts at: 112 2308 2435 112
Size: 2196 127 1996
Fragments arranged by size:
2196 1996 127
MspA1I CmG'CkG
Cuts at: 318 477 654 1195 1430 1744 2104 2888 3133
Size: 159 177 541 235 314 360 784 245
Cuts at: 3133 318
Size: 1504
Fragments arranged by size:
1504 784 541 360 314 245 235 177
159
NaeI GCC'GGC
Cuts at: 2500 2500
Size: 4319
NarI GG'CG_CC
Cuts at: 1750 1998 1750
Size: 248 4071
NcoI C'CATG_G
Cuts at: 430 430
Size: 4319
NgoMIV G'CCGG_C
Cuts at: 2498 2498
Size: 4319
NspI r_CATG'y
Cuts at: 882 1071 1372 1872 3475 882
Size: 189 301 500 1603 1726
Fragments arranged by size:
1726 1603 500 301 189
PciI A'CATG_T
Cuts at: 1067 3471 1067
Size: 2404 1915
PflMI CCAn_nnn'nTGG
Cuts at: 1305 1305
Size: 4319
PfoI T'CCnGG_A
Cuts at: 845 845
Size: 4319
PmeI GTTT'AAAC
Cuts at: 1318 1318
Size: 4319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2765 2797 2765
Size: 32 4287
PshAI GACnn'nnGTC
Cuts at: 1024 1024
Size: 4319
PstI C_TGCA'G
Cuts at: 1142 1366 2051 1142
Size: 224 685 3410
Fragments arranged by size:
3410 685 224
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4319
PvuII CAG'CTG
Cuts at: 318 654 1195 1744 2104 318
Size: 336 541 549 360 2533
Fragments arranged by size:
2533 549 541 360 336
RsrII CG'GwC_CG
Cuts at: 2514 2514
Size: 4319
SacI G_AGCT'C
Cuts at: 422 1333 422
Size: 911 3408
SalI G'TCGA_C
Cuts at: 1027 1356 1027
Size: 329 3990
SapI GCTCTTCn'nnn_
Cuts at: 2342 2552 2342
Size: 210 4109
SbfI CC_TGCA'GG
Cuts at: 1366 1366
Size: 4319
ScaI AGT'ACT
Cuts at: 3836 3836
Size: 4319
SfcI C'TryA_G
Cuts at: 33 1138 1362 2047 3015 3206 33
Size: 1105 224 685 968 191 1146
Fragments arranged by size:
1146 1105 968 685 224 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 208 208
Size: 4319
SfoI GGC'GCC
Cuts at: 1751 1999 1751
Size: 248 4071
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
SmaI CCC'GGG
Cuts at: 1341 1341
Size: 4319
SmlI C'TyrA_G
Cuts at: 1474 2389 3103 3365 1474
Size: 915 714 262 2428
Fragments arranged by size:
2428 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4319
SphI G_CATG'C
Cuts at: 1372 1872 1372
Size: 500 3819
StuI AGG'CCT
Cuts at: 253 253
Size: 4319
StyI C'CwwG_G
Cuts at: 257 430 1453 1807 257
Size: 173 1023 354 2769
Fragments arranged by size:
2769 1023 354 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 582 742 1078 1902 2035 2494 2703 582
Size: 160 336 824 133 459 209 2198
Fragments arranged by size:
2198 824 459 336 209 160 133
TatI w'GTAC_w
Cuts at: 410 3834 410
Size: 3424 895
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3836 3868 3836
Size: 32 4287
Tth111I GACn'n_nGTC
Cuts at: 2116 2116
Size: 4319
XbaI T'CTAG_A
Cuts at: 1350 1350
Size: 4319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1011 1814 1011
Size: 803 3516
XmaI C'CCGG_G
Cuts at: 1339 1339
Size: 4319
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BmtI BsaXI BsgI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI