Restriction map for
pF1KB5684
(Circular) (Six-base) MAPSORT of: pF1KB5684.seq Check: 3122 from: 1 to: 4799
pF1KB5684 4799 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 403 1009 403
Size: 606 4193
AccI GT'mk_AC
Cuts at: 1837 1837
Size: 4799
Acc65I G'GTAC_C
Cuts at: 1815 1815
Size: 4799
AclI AA'CG_TT
Cuts at: 4639 4639
Size: 4799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1516 2624 3056 3409 4071 970
Size: 546 1108 432 353 662 1698
Fragments arranged by size:
1698 1108 662 546 432 353
AflIII A'CryG_T
Cuts at: 2151 3951 4232 2151
Size: 1800 281 2718
Fragments arranged by size:
2718 1800 281
AgeI A'CCGG_T
Cuts at: 1791 1791
Size: 4799
AlwNI CAG_nnn'CTG
Cuts at: 352 408 749 882 1154 1532 2104 3542 352
Size: 56 341 133 272 378 572 1438 1609
Fragments arranged by size:
1609 1438 572 378 341 272 133 56
ApaI G_GGCC'C
Cuts at: 1610 1610
Size: 4799
ApaLI G'TGCA_C
Cuts at: 549 3637 549
Size: 3088 1711
ApoI r'AATT_y
Cuts at: 825 1264 1297 1803 4152 4408 825
Size: 439 33 506 2349 256 1216
Fragments arranged by size:
2349 1216 506 439 256 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 4799
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4799
AvaI C'yCGr_G
Cuts at: 1734 1819 1734
Size: 85 4714
BamHI G'GATC_C
Cuts at: 1824 1824
Size: 4799
BanI G'GyrC_C
Cuts at: 391 541 805 938 1815 2229 2477 2512 391
Size: 150 264 133 877 414 248 35 2678
Fragments arranged by size:
2678 877 414 264 248 150 133 35
BanII G_rGCy'C
Cuts at: 131 1052 1445 1610 1813 2843 131
Size: 921 393 165 203 1030 2087
Fragments arranged by size:
2087 1030 921 393 203 165
BbeI G_GCGC'C
Cuts at: 545 2233 2481 545
Size: 1688 248 2863
Fragments arranged by size:
2863 1688 248
BbsI GAAGACnn'nnnn_
Cuts at: 153 153
Size: 4799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 430 464 787 821 430
Size: 34 323 34 4408
Fragments arranged by size:
4408 323 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2697 3753 4799 2697
Size: 1056 1046 2697
Fragments arranged by size:
2697 1056 1046
BclI T'GATC_A
Cuts at: 2319 2319
Size: 4799
BglII A'GATC_T
Cuts at: 211 2314 4351 211
Size: 2103 2037 659
Fragments arranged by size:
2103 2037 659
BlpI GC'TnA_GC
Cuts at: 1911 1911
Size: 4799
Bme1580I G_kGCm'C
Cuts at: 336 394 439 520 553 876 989 1368 1610
Size: 58 45 81 33 323 113 379 242
Cuts at: 1610 2424 2517 3641 336
Size: 814 93 1124 1494
Fragments arranged by size:
1494 1124 814 379 323 242 113 93
81 58 45 33
BmgBI CAC'GTC
Cuts at: 968 968
Size: 4799
BmrI ACTGGGnnnn_n'
Cuts at: 863 892 2011 2092 2427 4571 863
Size: 29 1119 81 335 2144 1091
Fragments arranged by size:
2144 1119 1091 335 81 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3166 3166
Size: 4799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 1607
Size: 4799
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1975 2854 3568 3866 1975
Size: 879 714 298 2908
Fragments arranged by size:
2908 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4503 4503
Size: 4799
BsaAI yAC'GTr
Cuts at: 485 521 945 2152 485
Size: 36 424 1207 3132
Fragments arranged by size:
3132 1207 424 36
BsaBI GATnn'nnATC
Cuts at: 6 2313 4350 6
Size: 2307 2037 455
Fragments arranged by size:
2307 2037 455
BsaHI Gr'CG_yC
Cuts at: 542 1628 2230 2478 3180 4676 542
Size: 1086 602 248 702 1496 665
Fragments arranged by size:
1496 1086 702 665 602 248
BsaWI w'CCGG_w
Cuts at: 1791 1988 2509 3187 3598 3745 4019 1791
Size: 197 521 678 411 147 274 2571
Fragments arranged by size:
2571 678 521 411 274 197 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1051 1081 1051
Size: 30 4769
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 218 1267 1270 218
Size: 1049 3 3747
Fragments arranged by size:
3747 1049 3
BseYI C'CCAG_C
Cuts at: 172 451 618 1703 2224 2982 3647 4055 172
Size: 279 167 1085 521 758 665 408 916
Fragments arranged by size:
1085 916 758 665 521 408 279 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1518 1518
Size: 4799
BsiEI CG_ry'CG
Cuts at: 104 1792 2341 2387 3617 104
Size: 1688 549 46 1230 1286
Fragments arranged by size:
1688 1286 1230 549 46
BsiHKAI G_wGCw'C
Cuts at: 290 529 553 1445 1813 2591 2781 3641 290
Size: 239 24 892 368 778 190 860 1448
Fragments arranged by size:
1448 892 860 778 368 239 190 24
Bsp1286I G_dGCh'C
Cuts at: 131 290 336 394 439 520 529 553 876
Size: 159 46 58 45 81 9 24 323
Cuts at: 876 989 1052 1368 1445 1610 1813 2424 2517
Size: 113 63 316 77 165 203 611 93
Cuts at: 2517 2591 2781 2843 3641 131
Size: 74 190 62 798 1289
Fragments arranged by size:
1289 798 611 323 316 203 190 165
159 113 93 81 77 74 63 62
58 46 45 24 9
BspHI T'CATG_A
Cuts at: 3231 3231
Size: 4799
BspMI ACCTGCnnnn'nnnn_
Cuts at: 403 1009 1421 1849 2063 2365 2746 403
Size: 606 412 428 214 302 381 2456
Fragments arranged by size:
2456 606 428 412 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1122 1491 1601 3091 3145 4629 4794 50
Size: 1072 369 110 1490 54 1484 165 55
Fragments arranged by size:
1490 1484 1072 369 165 110 55 54
BsrDI GCAATG_nn'
Cuts at: 122 1325 2711 122
Size: 1203 1386 2210
Fragments arranged by size:
2210 1386 1203
BsrFI r'CCGG_y
Cuts at: 1791 2797 2978 1791
Size: 1006 181 3612
Fragments arranged by size:
3612 1006 181
BsrGI T'GTAC_A
Cuts at: 1742 1742
Size: 4799
BssSI C'ACGA_G
Cuts at: 3070 3778 3070
Size: 708 4091
BstAPI GCAn_nnn'nTGC
Cuts at: 1394 2176 1394
Size: 782 4017
BstBI TT'CG_AA
Cuts at: 3160 3160
Size: 4799
BstEII G'GTnAC_C
Cuts at: 1752 1752
Size: 4799
BstXI CCAn_nnnn'nTGG
Cuts at: 618 618
Size: 4799
BstYI r'GATC_y
Cuts at: 1 211 1824 2291 2314 2649 2895 3201 3213
Size: 210 1613 467 23 335 246 306 12
Cuts at: 3213 3299 4351 1
Size: 86 1052 449
Fragments arranged by size:
1613 1052 467 449 335 306 246 210
86 23 12
BtgI C'CryG_G
Cuts at: 107 777 820 107
Size: 670 43 4086
Fragments arranged by size:
4086 670 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1047 2749 2930 3093 1047
Size: 1702 181 163 2753
Fragments arranged by size:
2753 1702 181 163
BtsI GCAGTG_nn'
Cuts at: 1176 2042 1176
Size: 866 3933
DraI TTT'AAA
Cuts at: 1798 1798
Size: 4799
DraIII CAC_nnn'GTG
Cuts at: 348 1394 348
Size: 1046 3753
DrdI GACnn_nn'nnGTC
Cuts at: 2505 3849 2505
Size: 1344 3455
EaeI y'GGCC_r
Cuts at: 1674 2384 2558 2949 2976 4321 1674
Size: 710 174 391 27 1345 2152
Fragments arranged by size:
2152 1345 710 391 174 27
EagI C'GGCC_G
Cuts at: 2384 2384
Size: 4799
EarI CTCTTCn'nnn_
Cuts at: 115 195 1057 2822 3032 115
Size: 80 862 1765 210 1882
Fragments arranged by size:
1882 1765 862 210 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 612 1187 1423 3007 3749 3895 4609 612
Size: 575 236 1584 742 146 714 802
Fragments arranged by size:
1584 802 742 714 575 236 146
EcoICRI GAG'CTC
Cuts at: 1443 1811 1443
Size: 368 4431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1516 1607 2624 3056 3409 4071 970
Size: 546 91 1017 432 353 662 1698
Fragments arranged by size:
1698 1017 662 546 432 353 91
EcoO109I rG'GnC_Cy
Cuts at: 405 1607 1651 1707 1938 405
Size: 1202 44 56 231 3266
Fragments arranged by size:
3266 1202 231 56 44
EcoRI G'AATT_C
Cuts at: 825 1297 1803 825
Size: 472 506 3821
Fragments arranged by size:
3821 506 472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 4799
FspI TGC'GCA
Cuts at: 2580 2580
Size: 4799
HaeII r_GCGC'y
Cuts at: 545 2233 2481 3711 4482 545
Size: 1688 248 1230 771 862
Fragments arranged by size:
1688 1230 862 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 646 678 973 1005 1051 1083 1456 1488 2641
Size: 32 295 32 46 32 373 32 1153
Cuts at: 2641 2671 2673 2703 646
Size: 30 2 30 2742
Fragments arranged by size:
2742 1153 373 295 46 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1838 1838
Size: 4799
KasI G'GCGC_C
Cuts at: 541 2229 2477 541
Size: 1688 248 2863
Fragments arranged by size:
2863 1688 248
KpnI G_GTAC'C
Cuts at: 1819 1819
Size: 4799
MluI A'CGCG_T
Cuts at: 4232 4232
Size: 4799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3558 3742 3558
Size: 184 4615
MscI TGG'CCA
Cuts at: 2560 2560
Size: 4799
MslI CAynn'nnrTG
Cuts at: 2788 2915 2788
Size: 127 4672
MspA1I CmG'CkG
Cuts at: 532 593 1341 1361 1419 1532 1703 1910 2224
Size: 61 748 20 58 113 171 207 314
Cuts at: 2224 2584 3368 3613 532
Size: 360 784 245 1718
Fragments arranged by size:
1718 784 748 360 314 245 207 171
113 61 58 20
NaeI GCC'GGC
Cuts at: 2980 2980
Size: 4799
NarI GG'CG_CC
Cuts at: 542 2230 2478 542
Size: 1688 248 2863
Fragments arranged by size:
2863 1688 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4799
NgoMIV G'CCGG_C
Cuts at: 2978 2978
Size: 4799
NspI r_CATG'y
Cuts at: 1578 1788 1852 2352 3955 1578
Size: 210 64 500 1603 2422
Fragments arranged by size:
2422 1603 500 210 64
PciI A'CATG_T
Cuts at: 3951 3951
Size: 4799
PflMI CCAn_nnn'nTGG
Cuts at: 882 935 882
Size: 53 4746
PfoI T'CCnGG_A
Cuts at: 1184 1184
Size: 4799
PmeI GTTT'AAAC
Cuts at: 1798 1798
Size: 4799
PmlI CAC'GTG
Cuts at: 485 521 945 485
Size: 36 424 4339
Fragments arranged by size:
4339 424 36
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 4767
PspOMI G'GGCC_C
Cuts at: 1606 1606
Size: 4799
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 622 654 622
Size: 32 4767
PstI C_TGCA'G
Cuts at: 232 646 718 1340 1418 1846 2531 232
Size: 414 72 622 78 428 685 2500
Fragments arranged by size:
2500 685 622 428 414 78 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4799
PvuII CAG'CTG
Cuts at: 1341 1419 1703 2224 2584 1341
Size: 78 284 521 360 3556
Fragments arranged by size:
3556 521 360 284 78
RsrII CG'GwC_CG
Cuts at: 2994 2994
Size: 4799
SacI G_AGCT'C
Cuts at: 1445 1813 1445
Size: 368 4431
SalI G'TCGA_C
Cuts at: 1836 1836
Size: 4799
SapI GCTCTTCn'nnn_
Cuts at: 1057 2822 3032 1057
Size: 1765 210 2824
Fragments arranged by size:
2824 1765 210
SbfI CC_TGCA'GG
Cuts at: 1846 1846
Size: 4799
ScaI AGT'ACT
Cuts at: 976 4316 976
Size: 3340 1459
SfcI C'TryA_G
Cuts at: 33 228 510 588 642 714 1336 1414 1842
Size: 195 282 78 54 72 622 78 428
Cuts at: 1842 2527 3495 3686 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 685 622 428 282 195 191
78 78 72 54
SfoI GGC'GCC
Cuts at: 543 2231 2479 543
Size: 1688 248 2863
Fragments arranged by size:
2863 1688 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4799
SmaI CCC'GGG
Cuts at: 1736 1821 1736
Size: 85 4714
SmlI C'TyrA_G
Cuts at: 1954 2869 3583 3845 1954
Size: 915 714 262 2908
Fragments arranged by size:
2908 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4799
SphI G_CATG'C
Cuts at: 1852 2352 1852
Size: 500 4299
SspI AAT'ATT
Cuts at: 310 310
Size: 4799
StuI AGG'CCT
Cuts at: 1468 1468
Size: 4799
StyI C'CwwG_G
Cuts at: 107 373 1748 1933 2287 107
Size: 266 1375 185 354 2619
Fragments arranged by size:
2619 1375 354 266 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2382 2515 2974 3183 2382
Size: 133 459 209 3998
Fragments arranged by size:
3998 459 209 133
TatI w'GTAC_w
Cuts at: 410 698 974 1016 1742 4314 410
Size: 288 276 42 726 2572 895
Fragments arranged by size:
2572 895 726 288 276 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 447 479 1422 1454 4316 4348 447
Size: 32 943 32 2862 32 898
Fragments arranged by size:
2862 943 898 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2596 2596
Size: 4799
XbaI T'CTAG_A
Cuts at: 1830 1830
Size: 4799
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2294 2294
Size: 4799
XmaI C'CCGG_G
Cuts at: 1734 1819 1734
Size: 85 4714
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BsmBI BspEI BssHII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PpuMI PshAI
PsiI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI