Restriction map for
pF1KB5678
(Circular) (Six-base) MAPSORT of: pF1KB5678.seq Check: 7657 from: 1 to: 5447
pF1KB5678 5447 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 2485 2485
Size: 5447
Acc65I G'GTAC_C
Cuts at: 2463 2463
Size: 5447
AclI AA'CG_TT
Cuts at: 5287 5287
Size: 5447
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1504 2192 3272 3704 4057 4719 1504
Size: 688 1080 432 353 662 2232
Fragments arranged by size:
2232 1080 688 662 432 353
AfeI AGC'GCT
Cuts at: 1588 1588
Size: 5447
AflII C'TTAA_G
Cuts at: 1558 1558
Size: 5447
AflIII A'CryG_T
Cuts at: 2799 4599 4880 2799
Size: 1800 281 3366
Fragments arranged by size:
3366 1800 281
AleI CACnn'nnGTG
Cuts at: 1573 1573
Size: 5447
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1984 2016 1984
Size: 32 5415
AlwNI CAG_nnn'CTG
Cuts at: 316 819 1431 1492 2752 4190 316
Size: 503 612 61 1260 1438 1573
Fragments arranged by size:
1573 1438 1260 612 503 61
ApaI G_GGCC'C
Cuts at: 577 912 2219 577
Size: 335 1307 3805
Fragments arranged by size:
3805 1307 335
ApaLI G'TGCA_C
Cuts at: 4285 4285
Size: 5447
ApoI r'AATT_y
Cuts at: 1675 2294 2451 4800 5056 1675
Size: 619 157 2349 256 2066
Fragments arranged by size:
2349 2066 619 256 157
AseI AT'TA_AT
Cuts at: 20 863 20
Size: 843 4604
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5447
AvaI C'yCGr_G
Cuts at: 1502 1738 2165 2467 1502
Size: 236 427 302 4482
Fragments arranged by size:
4482 427 302 236
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 741 774 741
Size: 33 5414
BamHI G'GATC_C
Cuts at: 1391 2472 1391
Size: 1081 4366
BanI G'GyrC_C
Cuts at: 1575 2416 2463 2877 3125 3160 1575
Size: 841 47 414 248 35 3862
Fragments arranged by size:
3862 841 414 248 47 35
BanII G_rGCy'C
Cuts at: 577 912 1075 2219 2461 3491 577
Size: 335 163 1144 242 1030 2533
Fragments arranged by size:
2533 1144 1030 335 242 163
BbeI G_GCGC'C
Cuts at: 2881 3129 2881
Size: 248 5199
BbsI GAAGACnn'nnnn_
Cuts at: 176 176
Size: 5447
BbvCI CC'TCA_GC
Cuts at: 155 2153 155
Size: 1998 3449
BciVI GTATCCnnnnn_n'
Cuts at: 3345 4401 5447 3345
Size: 1056 1046 3345
Fragments arranged by size:
3345 1056 1046
BclI T'GATC_A
Cuts at: 2967 2967
Size: 5447
BglI GCCn_nnn'nGGC
Cuts at: 143 547 721 916 1843 143
Size: 404 174 195 927 3747
Fragments arranged by size:
3747 927 404 195 174
BglII A'GATC_T
Cuts at: 212 1911 2962 4999 212
Size: 1699 1051 2037 660
Fragments arranged by size:
2037 1699 1051 660
BlpI GC'TnA_GC
Cuts at: 2559 2559
Size: 5447
Bme1580I G_kGCm'C
Cuts at: 419 577 912 1580 1976 2219 3072 3165 4289
Size: 158 335 668 396 243 853 93 1124
Cuts at: 4289 419
Size: 1577
Fragments arranged by size:
1577 1124 853 668 396 335 243 158
93
BmgBI CAC'GTC
Cuts at: 1066 1066
Size: 5447
BmrI ACTGGGnnnn_n'
Cuts at: 1105 1123 1141 1159 1768 2659 2740 3075 5219
Size: 18 18 18 609 891 81 335 2144
Cuts at: 5219 1105
Size: 1333
Fragments arranged by size:
2144 1333 891 609 335 81 18 18
18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3814 3814
Size: 5447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 951 1061 1277 2241 2322 841
Size: 110 110 216 964 81 3966
Fragments arranged by size:
3966 964 216 110 110 81
Bpu10I CC'TnA_GC
Cuts at: 155 2153 155
Size: 1998 3449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2623 3502 4216 4514 2623
Size: 879 714 298 3556
Fragments arranged by size:
3556 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 739 1795 2072 5151 739
Size: 1056 277 3079 1035
Fragments arranged by size:
3079 1056 1035 277
BsaAI yAC'GTr
Cuts at: 2800 2800
Size: 5447
BsaBI GATnn'nnATC
Cuts at: 6 1631 2961 4998 6
Size: 1625 1330 2037 455
Fragments arranged by size:
2037 1625 1330 455
BsaHI Gr'CG_yC
Cuts at: 1000 2878 3126 3828 5324 1000
Size: 1878 248 702 1496 1123
Fragments arranged by size:
1878 1496 1123 702 248
BsaWI w'CCGG_w
Cuts at: 2636 3157 3835 4246 4393 4667 2636
Size: 521 678 411 147 274 3416
Fragments arranged by size:
3416 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1886 1916 1984 2014 1886
Size: 30 68 30 5319
Fragments arranged by size:
5319 68 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 1783 130
Size: 1653 3794
BseYI C'CCAG_C
Cuts at: 2225 2872 3630 4295 4703 2225
Size: 647 758 665 408 2969
Fragments arranged by size:
2969 758 665 647 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 767 923 1478 2081 767
Size: 156 555 603 4133
Fragments arranged by size:
4133 603 555 156
BsiEI CG_ry'CG
Cuts at: 104 2989 3035 4265 104
Size: 2885 46 1230 1286
Fragments arranged by size:
2885 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 992 2461 3239 3429 4289 992
Size: 1469 778 190 860 2150
Fragments arranged by size:
2150 1469 860 778 190
BsmI GAATG_Cn'
Cuts at: 357 561 2334 357
Size: 204 1773 3470
Fragments arranged by size:
3470 1773 204
Bsp1286I G_dGCh'C
Cuts at: 419 577 912 992 1075 1580 1976 2219 2461
Size: 158 335 80 83 505 396 243 242
Cuts at: 2461 3072 3165 3239 3429 3491 4289 419
Size: 611 93 74 190 62 798 1577
Fragments arranged by size:
1577 798 611 505 396 335 243 242
190 158 93 83 80 74 62
BspHI T'CATG_A
Cuts at: 1820 3879 1820
Size: 2059 3388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1439 1457 2497 2711 3013 3394 1439
Size: 18 1040 214 302 381 3492
Fragments arranged by size:
3492 1040 381 302 214 18
BsrBI CCG'CTC
Cuts at: 50 2336 3739 3793 5277 5442 50
Size: 2286 1403 54 1484 165 55
Fragments arranged by size:
2286 1484 1403 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2057 3359 2057
Size: 1302 4145
BsrFI r'CCGG_y
Cuts at: 2359 3445 3626 2359
Size: 1086 181 4180
Fragments arranged by size:
4180 1086 181
BssSI C'ACGA_G
Cuts at: 2084 3718 4426 2084
Size: 1634 708 3105
Fragments arranged by size:
3105 1634 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1431 2824 1431
Size: 1393 4054
BstBI TT'CG_AA
Cuts at: 3808 3808
Size: 5447
BstXI CCAn_nnnn'nTGG
Cuts at: 291 621 1010 1100 1118 1136 1154 1172 1226
Size: 330 389 90 18 18 18 18 54
Cuts at: 1226 1244 1262 1280 1334 1352 1388 1421 291
Size: 18 18 18 54 18 36 33 4317
Fragments arranged by size:
4317 389 330 90 54 54 36 33
18 18 18 18 18 18 18 18
BstYI r'GATC_y
Cuts at: 1 212 1391 1911 2280 2472 2939 2962 3297
Size: 211 1179 520 369 192 467 23 335
Cuts at: 3297 3543 3849 3861 3947 4999 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1179 1052 520 467 449 369 335 306
246 211 192 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1023 1023
Size: 5447
BtgI C'CryG_G
Cuts at: 107 107
Size: 5447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3397 3578 3741 3397
Size: 181 163 5103
Fragments arranged by size:
5103 181 163
BtsI GCAGTG_nn'
Cuts at: 897 1339 2690 897
Size: 442 1351 3654
Fragments arranged by size:
3654 1351 442
DraI TTT'AAA
Cuts at: 342 2446 342
Size: 2104 3343
DrdI GACnn_nn'nnGTC
Cuts at: 933 3153 4497 933
Size: 2220 1344 1883
Fragments arranged by size:
2220 1883 1344
EaeI y'GGCC_r
Cuts at: 623 722 1138 1174 1210 1318 1336 1354 1372
Size: 99 416 36 36 108 18 18 18
Cuts at: 1372 1405 3032 3206 3597 3624 4969 623
Size: 33 1627 174 391 27 1345 1101
Fragments arranged by size:
1627 1345 1101 416 391 174 108 99
36 36 33 27 18 18 18
EagI C'GGCC_G
Cuts at: 3032 3032
Size: 5447
EarI CTCTTCn'nnn_
Cuts at: 875 3470 3680 875
Size: 2595 210 2642
Fragments arranged by size:
2642 2595 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3655 4397 4543 5257 3655
Size: 742 146 714 3845
Fragments arranged by size:
3845 742 714 146
EcoICRI GAG'CTC
Cuts at: 2459 2459
Size: 5447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 951 1061 1277 1504 2192 2241 2322 3272
Size: 110 110 216 227 688 49 81 950
Cuts at: 3272 3704 4057 4719 841
Size: 432 353 662 1569
Fragments arranged by size:
1569 950 688 662 432 353 227 216
110 110 81 49
EcoNI CCTnn'n_nnAGG
Cuts at: 1519 1855 1519
Size: 336 5111
EcoO109I rG'GnC_Cy
Cuts at: 675 851 909 926 1288 1306 2010 2216 2586
Size: 176 58 17 362 18 704 206 370
Cuts at: 2586 675
Size: 3536
Fragments arranged by size:
3536 704 370 362 206 176 58 18
17
EcoRI G'AATT_C
Cuts at: 2294 2451 2294
Size: 157 5290
EcoRV GAT'ATC
Cuts at: 1608 1608
Size: 5447
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 354 456 3466 354
Size: 102 3010 2335
Fragments arranged by size:
3010 2335 102
FspI TGC'GCA
Cuts at: 3228 3228
Size: 5447
HaeII r_GCGC'y
Cuts at: 179 1590 2881 3129 4359 5130 179
Size: 1411 1291 248 1230 771 496
Fragments arranged by size:
1411 1291 1230 771 496 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1595 1627 1886 1918 2194 2226 2272 2304 3289
Size: 32 259 32 276 32 46 32 985
Cuts at: 3289 3319 3321 3351 1595
Size: 30 2 30 3691
Fragments arranged by size:
3691 985 276 259 46 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2486 2486
Size: 5447
KasI G'GCGC_C
Cuts at: 2877 3125 2877
Size: 248 5199
KpnI G_GTAC'C
Cuts at: 2467 2467
Size: 5447
MluI A'CGCG_T
Cuts at: 4880 4880
Size: 5447
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4206 4390 4206
Size: 184 5263
MscI TGG'CCA
Cuts at: 625 724 1140 1176 1338 1356 1374 1407 3208
Size: 99 416 36 162 18 18 33 1801
Cuts at: 3208 625
Size: 2864
Fragments arranged by size:
2864 1801 416 162 99 36 33 18
18
MslI CAynn'nnrTG
Cuts at: 1242 1260 1573 1819 2327 3436 3563 1242
Size: 18 313 246 508 1109 127 3126
Fragments arranged by size:
3126 1109 508 313 246 127 18
MspA1I CmG'CkG
Cuts at: 250 316 685 1399 2152 2558 2872 3232 4016
Size: 66 369 714 753 406 314 360 784
Cuts at: 4016 4261 250
Size: 245 1436
Fragments arranged by size:
1436 784 753 714 406 369 360 314
245 66
NaeI GCC'GGC
Cuts at: 3628 3628
Size: 5447
NarI GG'CG_CC
Cuts at: 2878 3126 2878
Size: 248 5199
NcoI C'CATG_G
Cuts at: 107 107
Size: 5447
NgoMIV G'CCGG_C
Cuts at: 3626 3626
Size: 5447
NspI r_CATG'y
Cuts at: 1658 2500 3000 4603 1658
Size: 842 500 1603 2502
Fragments arranged by size:
2502 1603 842 500
PciI A'CATG_T
Cuts at: 4599 4599
Size: 5447
PflMI CCAn_nnn'nTGG
Cuts at: 1190 1370 1190
Size: 180 5267
PfoI T'CCnGG_A
Cuts at: 826 826
Size: 5447
PmeI GTTT'AAAC
Cuts at: 2446 2446
Size: 5447
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1984 2016 3893 3925 1984
Size: 32 1877 32 3506
Fragments arranged by size:
3506 1877 32 32
PpuMI rG'GwC_Cy
Cuts at: 851 926 1288 1306 851
Size: 75 362 18 4992
Fragments arranged by size:
4992 362 75 18
PspOMI G'GGCC_C
Cuts at: 573 908 2215 573
Size: 335 1307 3805
Fragments arranged by size:
3805 1307 335
PstI C_TGCA'G
Cuts at: 924 2118 2151 2494 3179 924
Size: 1194 33 343 685 3192
Fragments arranged by size:
3192 1194 685 343 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5447
PvuII CAG'CTG
Cuts at: 250 316 685 2152 2872 3232 250
Size: 66 369 1467 720 360 2465
Fragments arranged by size:
2465 1467 720 369 360 66
RsrII CG'GwC_CG
Cuts at: 3642 3642
Size: 5447
SacI G_AGCT'C
Cuts at: 2461 2461
Size: 5447
SalI G'TCGA_C
Cuts at: 2484 2484
Size: 5447
SapI GCTCTTCn'nnn_
Cuts at: 3470 3680 3470
Size: 210 5237
SbfI CC_TGCA'GG
Cuts at: 2494 2494
Size: 5447
ScaI AGT'ACT
Cuts at: 1549 4964 1549
Size: 3415 2032
SfcI C'TryA_G
Cuts at: 33 920 1962 2114 2147 2490 3175 4143 4334
Size: 887 1042 152 33 343 685 968 191
Cuts at: 4334 33
Size: 1146
Fragments arranged by size:
1146 1042 968 887 685 343 191 152
33
SfiI GGCCn_nnn'nGGCC
Cuts at: 547 721 547
Size: 174 5273
SfoI GGC'GCC
Cuts at: 2879 3127 2879
Size: 248 5199
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5447
SmaI CCC'GGG
Cuts at: 2167 2469 2167
Size: 302 5145
SmlI C'TyrA_G
Cuts at: 1558 2602 3517 4231 4493 1558
Size: 1044 915 714 262 2512
Fragments arranged by size:
2512 1044 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5447
SphI G_CATG'C
Cuts at: 1658 2500 3000 1658
Size: 842 500 4105
Fragments arranged by size:
4105 842 500
SrfI GCCC'GGGC
Cuts at: 2167 2167
Size: 5447
StuI AGG'CCT
Cuts at: 160 327 636 2125 160
Size: 167 309 1489 3482
Fragments arranged by size:
3482 1489 309 167
StyI C'CwwG_G
Cuts at: 107 407 431 1905 2126 2581 2935 107
Size: 300 24 1474 221 455 354 2619
Fragments arranged by size:
2619 1474 455 354 300 221 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 763 1777 1794 1798 3030 3163 3622 3831 763
Size: 1014 17 4 1232 133 459 209 2379
Fragments arranged by size:
2379 1232 1014 459 209 133 17 4
TatI w'GTAC_w
Cuts at: 1547 4962 1547
Size: 3415 2032
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4964 4996 4964
Size: 32 5415
Tth111I GACn'n_nGTC
Cuts at: 1880 3244 1880
Size: 1364 4083
XbaI T'CTAG_A
Cuts at: 2478 2478
Size: 5447
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 567 2306 2942 567
Size: 1739 636 3072
Fragments arranged by size:
3072 1739 636
XmaI C'CCGG_G
Cuts at: 2165 2467 2165
Size: 302 5145
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AgeI AhdI AlfI AscI AvrII BcgI
BfrBI BmtI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SspI SwaI XhoI XmnI ZraI