Restriction map for
pF1KB5677
(Circular) (Six-base) MAPSORT of: pF1KB5677.seq Check: 3415 from: 1 to: 4124
pF1KB5677 4124 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1162 1162
Size: 4124
Acc65I G'GTAC_C
Cuts at: 358 1140 358
Size: 782 3342
AclI AA'CG_TT
Cuts at: 3964 3964
Size: 4124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 494 1949 2381 3128 3396 137
Size: 357 1455 432 747 268 865
Fragments arranged by size:
1455 865 747 432 357 268
AflIII A'CryG_T
Cuts at: 1476 2580 3557 1476
Size: 1104 977 2043
Fragments arranged by size:
2043 1104 977
AgeI A'CCGG_T
Cuts at: 2512 2512
Size: 4124
AlwNI CAG_nnn'CTG
Cuts at: 1429 2996 1429
Size: 1567 2557
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 196 238 434 546 701 832 1045 1128 3477
Size: 42 196 112 155 131 213 83 2349
Cuts at: 3477 3733 196
Size: 256 587
Fragments arranged by size:
2349 587 256 213 196 155 131 112
83 42
AseI AT'TA_AT
Cuts at: 20 908 20
Size: 888 3236
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 1144 1144
Size: 4124
BamHI G'GATC_C
Cuts at: 1149 1149
Size: 4124
BanI G'GyrC_C
Cuts at: 358 739 1140 1554 1802 1837 358
Size: 381 401 414 248 35 2645
Fragments arranged by size:
2645 414 401 381 248 35
BanII G_rGCy'C
Cuts at: 1138 2168 1138
Size: 1030 3094
BbeI G_GCGC'C
Cuts at: 1558 1806 1558
Size: 248 3876
BbsI GAAGACnn'nnnn_
Cuts at: 453 453
Size: 4124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 373 407 373
Size: 34 4090
BciVI GTATCCnnnnn_n'
Cuts at: 2022 2783 4124 2022
Size: 761 1341 2022
Fragments arranged by size:
2022 1341 761
BclI T'GATC_A
Cuts at: 300 1644 300
Size: 1344 2780
BglII A'GATC_T
Cuts at: 688 1639 3676 688
Size: 951 2037 1136
Fragments arranged by size:
2037 1136 951
BlpI GC'TnA_GC
Cuts at: 1236 1236
Size: 4124
Bme1580I G_kGCm'C
Cuts at: 1749 1842 2898 1749
Size: 93 1056 2975
Fragments arranged by size:
2975 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1336 1417 1752 3896 1336
Size: 81 335 2144 1564
Fragments arranged by size:
2144 1564 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 566
Size: 4124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 2179 2671 2969 1300
Size: 879 492 298 2455
Fragments arranged by size:
2455 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3828 3828
Size: 4124
BsaAI yAC'GTr
Cuts at: 1477 1477
Size: 4124
BsaBI GATnn'nnATC
Cuts at: 6 494 1638 3675 6
Size: 488 1144 2037 455
Fragments arranged by size:
2037 1144 488 455
BsaHI Gr'CG_yC
Cuts at: 1555 1803 2505 4001 1555
Size: 248 702 1496 1678
Fragments arranged by size:
1678 1496 702 248
BsaWI w'CCGG_w
Cuts at: 204 365 1313 1834 2512 2786 2933 3344 204
Size: 161 948 521 678 274 147 411 984
Fragments arranged by size:
984 948 678 521 411 274 161 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 304 334 304
Size: 30 4094
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 212 344 990 212
Size: 132 646 3346
Fragments arranged by size:
3346 646 132
BseYI C'CCAG_C
Cuts at: 1549 2307 2884 3380 1549
Size: 758 577 496 2293
Fragments arranged by size:
2293 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1666 1712 2920 104
Size: 1562 46 1208 1308
Fragments arranged by size:
1562 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1138 1916 2106 2898 1138
Size: 778 190 792 2364
Fragments arranged by size:
2364 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1138 1749 1842 1916 2106 2168 2898 1138
Size: 611 93 74 190 62 730 2364
Fragments arranged by size:
2364 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 204 3344 204
Size: 3140 984
BspHI T'CATG_A
Cuts at: 591 3300 591
Size: 2709 1415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1174 1388 1690 2071 1174
Size: 214 302 381 3227
Fragments arranged by size:
3227 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2416 2470 3954 4119 50
Size: 2366 54 1484 165 55
Fragments arranged by size:
2366 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2036 2036
Size: 4124
BsrFI r'CCGG_y
Cuts at: 2122 2303 2512 2122
Size: 181 209 3734
Fragments arranged by size:
3734 209 181
BssSI C'ACGA_G
Cuts at: 2395 2753 2395
Size: 358 3766
BstAPI GCAn_nnn'nTGC
Cuts at: 1501 1501
Size: 4124
BstBI TT'CG_AA
Cuts at: 2485 2485
Size: 4124
BstEII G'GTnAC_C
Cuts at: 1030 1030
Size: 4124
BstYI r'GATC_y
Cuts at: 1 576 688 1149 1616 1639 1974 2220 3232
Size: 575 112 461 467 23 335 246 1012
Cuts at: 3232 3318 3330 3676 1
Size: 86 12 346 449
Fragments arranged by size:
1012 575 467 461 449 346 335 246
112 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 224 224
Size: 4124
BtgI C'CryG_G
Cuts at: 107 107
Size: 4124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2074 2255 2418 2074
Size: 181 163 3780
Fragments arranged by size:
3780 181 163
BtsI GCAGTG_nn'
Cuts at: 1367 1367
Size: 4124
DraI TTT'AAA
Cuts at: 243 837 1123 243
Size: 594 286 3244
Fragments arranged by size:
3244 594 286
DrdI GACnn_nn'nnGTC
Cuts at: 1830 2688 1830
Size: 858 3266
EaeI y'GGCC_r
Cuts at: 1709 1883 2274 2301 3646 1709
Size: 174 391 27 1345 2187
Fragments arranged by size:
2187 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1709 1709
Size: 4124
EarI CTCTTCn'nnn_
Cuts at: 608 2147 2357 608
Size: 1539 210 2375
Fragments arranged by size:
2375 1539 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 744 2332 2642 2788 3934 744
Size: 1588 310 146 1146 934
Fragments arranged by size:
1588 1146 934 310 146
EcoICRI GAG'CTC
Cuts at: 1136 1136
Size: 4124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 494 566 1949 2381 3128 3396 137
Size: 357 72 1383 432 747 268 865
Fragments arranged by size:
1383 865 747 432 357 268 72
EcoO109I rG'GnC_Cy
Cuts at: 1263 1263
Size: 4124
EcoRI G'AATT_C
Cuts at: 546 1128 546
Size: 582 3542
EcoRV GAT'ATC
Cuts at: 532 532
Size: 4124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1111 2143 1111
Size: 1032 3092
FspI TGC'GCA
Cuts at: 1905 1905
Size: 4124
HaeII r_GCGC'y
Cuts at: 1558 1806 2828 3807 1558
Size: 248 1022 979 1875
Fragments arranged by size:
1875 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1057 1089 1966 1996 1998 2028 1057
Size: 32 877 30 2 30 3153
Fragments arranged by size:
3153 877 32 30 30 2
HincII GTy'rAC
Cuts at: 1163 1163
Size: 4124
HindIII A'AGCT_T
Cuts at: 257 257
Size: 4124
KasI G'GCGC_C
Cuts at: 1554 1802 1554
Size: 248 3876
KpnI G_GTAC'C
Cuts at: 362 1144 362
Size: 782 3342
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 446 2795 2979 446
Size: 2349 184 1591
Fragments arranged by size:
2349 1591 184
MscI TGG'CCA
Cuts at: 1885 1885
Size: 4124
MslI CAynn'nnrTG
Cuts at: 940 2113 2240 940
Size: 1173 127 2824
Fragments arranged by size:
2824 1173 127
MspA1I CmG'CkG
Cuts at: 1235 1549 1909 2922 3167 1235
Size: 314 360 1013 245 2192
Fragments arranged by size:
2192 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 4124
NarI GG'CG_CC
Cuts at: 1555 1803 1555
Size: 248 3876
NcoI C'CATG_G
Cuts at: 107 107
Size: 4124
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 4124
NspI r_CATG'y
Cuts at: 1177 1677 2584 1177
Size: 500 907 2717
Fragments arranged by size:
2717 907 500
PciI A'CATG_T
Cuts at: 2580 2580
Size: 4124
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 4092
PstI C_TGCA'G
Cuts at: 1171 1856 1171
Size: 685 3439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 1549 1909 1549
Size: 360 3764
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 1138 1138
Size: 4124
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 1094 3641 1094
Size: 2547 1577
SfcI C'TryA_G
Cuts at: 33 1167 1852 2845 3036 33
Size: 1134 685 993 191 1121
Fragments arranged by size:
1134 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1556 1804 1556
Size: 248 3876
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 1146 1146
Size: 4124
SmlI C'TyrA_G
Cuts at: 1279 2194 2686 2948 1279
Size: 915 492 262 2455
Fragments arranged by size:
2455 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4124
SphI G_CATG'C
Cuts at: 1177 1677 1177
Size: 500 3624
StyI C'CwwG_G
Cuts at: 107 1258 1612 107
Size: 1151 354 2619
Fragments arranged by size:
2619 1151 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 445 1707 1840 2299 2508 445
Size: 1262 133 459 209 2061
Fragments arranged by size:
2061 1262 459 209 133
TatI w'GTAC_w
Cuts at: 1092 3639 1092
Size: 2547 1577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4092
Tth111I GACn'n_nGTC
Cuts at: 1921 1921
Size: 4124
XbaI T'CTAG_A
Cuts at: 1155 1155
Size: 4124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1619 1619
Size: 4124
XmaI C'CCGG_G
Cuts at: 1144 1144
Size: 4124
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI