Restriction map for
pF1KB5673
(Circular) (Six-base) MAPSORT of: pF1KB5673.seq Check: 1369 from: 1 to: 4763
pF1KB5673 4763 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 688 1801 688
Size: 1113 3650
Acc65I G'GTAC_C
Cuts at: 1779 1779
Size: 4763
AclI AA'CG_TT
Cuts at: 4603 4603
Size: 4763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1161 2588 3020 3767 4035 1161
Size: 1427 432 747 268 1889
Fragments arranged by size:
1889 1427 747 432 268
AfeI AGC'GCT
Cuts at: 1182 1182
Size: 4763
AflIII A'CryG_T
Cuts at: 536 1751 2115 3219 4196 536
Size: 1215 364 1104 977 1103
Fragments arranged by size:
1215 1104 1103 977 364
AgeI A'CCGG_T
Cuts at: 3151 3151
Size: 4763
AlwNI CAG_nnn'CTG
Cuts at: 2068 3635 2068
Size: 1567 3196
ApaLI G'TGCA_C
Cuts at: 3533 3533
Size: 4763
ApoI r'AATT_y
Cuts at: 646 945 1767 4116 4372 646
Size: 299 822 2349 256 1037
Fragments arranged by size:
2349 1037 822 299 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4763
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
AvaI C'yCGr_G
Cuts at: 935 1783 935
Size: 848 3915
BamHI G'GATC_C
Cuts at: 1788 1788
Size: 4763
BanI G'GyrC_C
Cuts at: 1779 2193 2441 2476 1779
Size: 414 248 35 4066
Fragments arranged by size:
4066 414 248 35
BanII G_rGCy'C
Cuts at: 1175 1777 2807 1175
Size: 602 1030 3131
Fragments arranged by size:
3131 1030 602
BbeI G_GCGC'C
Cuts at: 2197 2445 2197
Size: 248 4515
BbsI GAAGACnn'nnnn_
Cuts at: 185 185
Size: 4763
BbvCI CC'TCA_GC
Cuts at: 568 568
Size: 4763
BciVI GTATCCnnnnn_n'
Cuts at: 2661 3422 4763 2661
Size: 761 1341 2661
Fragments arranged by size:
2661 1341 761
BclI T'GATC_A
Cuts at: 2283 2283
Size: 4763
BglII A'GATC_T
Cuts at: 2278 4315 2278
Size: 2037 2726
BlpI GC'TnA_GC
Cuts at: 1875 1875
Size: 4763
Bme1580I G_kGCm'C
Cuts at: 1146 2388 2481 3537 1146
Size: 1242 93 1056 2372
Fragments arranged by size:
2372 1242 1056 93
BmgBI CAC'GTC
Cuts at: 1340 1681 1340
Size: 341 4422
BmrI ACTGGGnnnn_n'
Cuts at: 1975 2056 2391 4535 1975
Size: 81 335 2144 2203
Fragments arranged by size:
2203 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 467 1666 3130 467
Size: 1199 1464 2100
Fragments arranged by size:
2100 1464 1199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 437
Size: 4763
Bpu10I CC'TnA_GC
Cuts at: 568 953 568
Size: 385 4378
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 1530 1649 1939 2818 3310 3608 634
Size: 896 119 290 879 492 298 1789
Fragments arranged by size:
1789 896 879 492 298 290 119
BsaI GGTCTCn'nnnn_
Cuts at: 4467 4467
Size: 4763
BsaAI yAC'GTr
Cuts at: 1752 2116 1752
Size: 364 4399
BsaBI GATnn'nnATC
Cuts at: 6 1440 2277 4314 6
Size: 1434 837 2037 455
Fragments arranged by size:
2037 1434 837 455
BsaHI Gr'CG_yC
Cuts at: 2194 2442 3144 4640 2194
Size: 248 702 1496 2317
Fragments arranged by size:
2317 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1952 2473 3151 3425 3572 3983 1952
Size: 521 678 274 147 411 2732
Fragments arranged by size:
2732 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 541 541
Size: 4763
BseYI C'CCAG_C
Cuts at: 2188 2946 3523 4019 2188
Size: 758 577 496 2932
Fragments arranged by size:
2932 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2305 2351 3559 104
Size: 2201 46 1208 1308
Fragments arranged by size:
2201 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 450 1532 1541 1777 2555 2745 3537 450
Size: 1082 9 236 778 190 792 1676
Fragments arranged by size:
1676 1082 792 778 236 190 9
Bsp1286I G_dGCh'C
Cuts at: 450 1146 1175 1532 1541 1777 2388 2481 2555
Size: 696 29 357 9 236 611 93 74
Cuts at: 2555 2745 2807 3537 450
Size: 190 62 730 1676
Fragments arranged by size:
1676 730 696 611 357 236 190 93
74 62 29 9
BspEI T'CCGG_A
Cuts at: 3983 3983
Size: 4763
BspHI T'CATG_A
Cuts at: 788 3939 788
Size: 3151 1612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1813 2027 2329 2710 1813
Size: 214 302 381 3866
Fragments arranged by size:
3866 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3055 3109 4593 4758 50
Size: 3005 54 1484 165 55
Fragments arranged by size:
3005 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1644 2675 1644
Size: 1031 3732
BsrFI r'CCGG_y
Cuts at: 140 2761 2942 3151 140
Size: 2621 181 209 1752
Fragments arranged by size:
2621 1752 209 181
BssSI C'ACGA_G
Cuts at: 1184 3034 3392 1184
Size: 1850 358 2555
Fragments arranged by size:
2555 1850 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2140 2140
Size: 4763
BstBI TT'CG_AA
Cuts at: 3124 3124
Size: 4763
BstXI CCAn_nnnn'nTGG
Cuts at: 1713 1736 1713
Size: 23 4740
BstYI r'GATC_y
Cuts at: 1 1788 2255 2278 2613 2859 3871 3957 3969
Size: 1787 467 23 335 246 1012 86 12
Cuts at: 3969 4315 1
Size: 346 449
Fragments arranged by size:
1787 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 167 167
Size: 4763
BtgI C'CryG_G
Cuts at: 107 774 107
Size: 667 4096
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2713 2894 3057 2713
Size: 181 163 4419
Fragments arranged by size:
4419 181 163
BtsI GCAGTG_nn'
Cuts at: 847 1263 2006 847
Size: 416 743 3604
Fragments arranged by size:
3604 743 416
DraI TTT'AAA
Cuts at: 1762 1762
Size: 4763
DrdI GACnn_nn'nnGTC
Cuts at: 2469 3327 2469
Size: 858 3905
EaeI y'GGCC_r
Cuts at: 1726 2348 2522 2913 2940 4285 1726
Size: 622 174 391 27 1345 2204
Fragments arranged by size:
2204 1345 622 391 174 27
EagI C'GGCC_G
Cuts at: 2348 2348
Size: 4763
EarI CTCTTCn'nnn_
Cuts at: 113 775 1243 1537 1546 2786 2996 113
Size: 662 468 294 9 1240 210 1880
Fragments arranged by size:
1880 1240 662 468 294 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2971 3281 3427 4573 2971
Size: 310 146 1146 3161
Fragments arranged by size:
3161 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1775 1775
Size: 4763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 1161 2588 3020 3767 4035 437
Size: 724 1427 432 747 268 1165
Fragments arranged by size:
1427 1165 747 724 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1902 1902
Size: 4763
EcoRI G'AATT_C
Cuts at: 1767 1767
Size: 4763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4763
FspI TGC'GCA
Cuts at: 2544 2544
Size: 4763
HaeII r_GCGC'y
Cuts at: 486 1184 2197 2445 3467 4446 486
Size: 698 1013 248 1022 979 803
Fragments arranged by size:
1022 1013 979 803 698 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 735 750 767 782 2605 2635 2637
Size: 32 591 15 17 15 1823 30 2
Cuts at: 2637 2667 112
Size: 30 2208
Fragments arranged by size:
2208 1823 591 32 30 30 17 15
15 2
HincII GTy'rAC
Cuts at: 269 990 1802 269
Size: 721 812 3230
Fragments arranged by size:
3230 812 721
KasI G'GCGC_C
Cuts at: 2193 2441 2193
Size: 248 4515
KpnI G_GTAC'C
Cuts at: 1783 1783
Size: 4763
MluI A'CGCG_T
Cuts at: 4196 4196
Size: 4763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1373 1567 3434 3618 1373
Size: 194 1867 184 2518
Fragments arranged by size:
2518 1867 194 184
MscI TGG'CCA
Cuts at: 2524 2524
Size: 4763
MslI CAynn'nnrTG
Cuts at: 326 1490 2752 2879 326
Size: 1164 1262 127 2210
Fragments arranged by size:
2210 1262 1164 127
MspA1I CmG'CkG
Cuts at: 756 1874 2188 2548 3561 3806 756
Size: 1118 314 360 1013 245 1713
Fragments arranged by size:
1713 1118 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2944 2944
Size: 4763
NarI GG'CG_CC
Cuts at: 2194 2442 2194
Size: 248 4515
NcoI C'CATG_G
Cuts at: 107 774 107
Size: 667 4096
NdeI CA'TA_TG
Cuts at: 828 828
Size: 4763
NgoMIV G'CCGG_C
Cuts at: 2942 2942
Size: 4763
NspI r_CATG'y
Cuts at: 540 1816 2316 3223 540
Size: 1276 500 907 2080
Fragments arranged by size:
2080 1276 907 500
PciI A'CATG_T
Cuts at: 536 3219 536
Size: 2683 2080
PfoI T'CCnGG_A
Cuts at: 1735 1735
Size: 4763
PmeI GTTT'AAAC
Cuts at: 1762 1762
Size: 4763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 4731
PstI C_TGCA'G
Cuts at: 1810 2495 1810
Size: 685 4078
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4763
PvuII CAG'CTG
Cuts at: 2188 2548 2188
Size: 360 4403
RsrII CG'GwC_CG
Cuts at: 2958 2958
Size: 4763
SacI G_AGCT'C
Cuts at: 1777 1777
Size: 4763
SalI G'TCGA_C
Cuts at: 1800 1800
Size: 4763
SapI GCTCTTCn'nnn_
Cuts at: 1537 1546 2786 2996 1537
Size: 9 1240 210 3304
Fragments arranged by size:
3304 1240 210 9
SbfI CC_TGCA'GG
Cuts at: 1810 1810
Size: 4763
ScaI AGT'ACT
Cuts at: 4280 4280
Size: 4763
SexAI A'CCwGG_T
Cuts at: 667 667
Size: 4763
SfcI C'TryA_G
Cuts at: 33 735 983 1478 1806 2491 3484 3675 33
Size: 702 248 495 328 685 993 191 1121
Fragments arranged by size:
1121 993 702 685 495 328 248 191
SfoI GGC'GCC
Cuts at: 2195 2443 2195
Size: 248 4515
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
SmaI CCC'GGG
Cuts at: 1785 1785
Size: 4763
SmlI C'TyrA_G
Cuts at: 613 1509 1664 1918 2833 3325 3587 613
Size: 896 155 254 915 492 262 1789
Fragments arranged by size:
1789 915 896 492 262 254 155
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4763
SphI G_CATG'C
Cuts at: 1816 2316 1816
Size: 500 4263
StyI C'CwwG_G
Cuts at: 107 774 1496 1897 2251 107
Size: 667 722 401 354 2619
Fragments arranged by size:
2619 722 667 401 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 103 368 2346 2479 2938 3147 103
Size: 265 1978 133 459 209 1719
Fragments arranged by size:
1978 1719 459 265 209 133
TatI w'GTAC_w
Cuts at: 1001 1118 1482 4278 1001
Size: 117 364 2796 1486
Fragments arranged by size:
2796 1486 364 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 593 625 4280 4312 593
Size: 32 3655 32 1044
Fragments arranged by size:
3655 1044 32 32
Tth111I GACn'n_nGTC
Cuts at: 2560 2560
Size: 4763
XbaI T'CTAG_A
Cuts at: 1794 1794
Size: 4763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2258 2258
Size: 4763
XmaI C'CCGG_G
Cuts at: 1783 1783
Size: 4763
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI