Restriction map for
pF1KB5672
(Circular) (Six-base) MAPSORT of: pF1KB5672.seq Check: 144 from: 1 to: 4721
pF1KB5672 4721 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1759 1759
Size: 4721
Acc65I G'GTAC_C
Cuts at: 1352 1737 1352
Size: 385 4336
AclI AA'CG_TT
Cuts at: 4561 4561
Size: 4721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 526 876 1252 2546 2978 3331 3993 200
Size: 326 350 376 1294 432 353 662 928
Fragments arranged by size:
1294 928 662 432 376 353 350 326
AflIII A'CryG_T
Cuts at: 2073 3873 4154 2073
Size: 1800 281 2640
Fragments arranged by size:
2640 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 939 939
Size: 4721
AlwNI CAG_nnn'CTG
Cuts at: 610 2026 3464 610
Size: 1416 1438 1867
Fragments arranged by size:
1867 1438 1416
ApaI G_GGCC'C
Cuts at: 807 933 807
Size: 126 4595
ApaLI G'TGCA_C
Cuts at: 3559 3559
Size: 4721
ApoI r'AATT_y
Cuts at: 415 1725 4074 4330 415
Size: 1310 2349 256 806
Fragments arranged by size:
2349 1310 806 256
AseI AT'TA_AT
Cuts at: 20 275 20
Size: 255 4466
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
AvaI C'yCGr_G
Cuts at: 1741 1741
Size: 4721
BamHI G'GATC_C
Cuts at: 1746 1746
Size: 4721
BanI G'GyrC_C
Cuts at: 1352 1737 2151 2399 2434 1352
Size: 385 414 248 35 3639
Fragments arranged by size:
3639 414 385 248 35
BanII G_rGCy'C
Cuts at: 127 328 552 807 933 1215 1735 2765 127
Size: 201 224 255 126 282 520 1030 2083
Fragments arranged by size:
2083 1030 520 282 255 224 201 126
BbeI G_GCGC'C
Cuts at: 2155 2403 2155
Size: 248 4473
BbsI GAAGACnn'nnnn_
Cuts at: 295 1628 295
Size: 1333 3388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1401 1435 1401
Size: 34 4687
BciVI GTATCCnnnnn_n'
Cuts at: 2619 3675 4721 2619
Size: 1056 1046 2619
Fragments arranged by size:
2619 1056 1046
BclI T'GATC_A
Cuts at: 347 2241 347
Size: 1894 2827
BglII A'GATC_T
Cuts at: 1574 2236 4273 1574
Size: 662 2037 2022
Fragments arranged by size:
2037 2022 662
BlpI GC'TnA_GC
Cuts at: 1833 1833
Size: 4721
Bme1580I G_kGCm'C
Cuts at: 678 807 933 2346 2439 3563 678
Size: 129 126 1413 93 1124 1836
Fragments arranged by size:
1836 1413 1124 129 126 93
BmrI ACTGGGnnnn_n'
Cuts at: 344 688 1085 1375 1933 2014 2349 4493 344
Size: 344 397 290 558 81 335 2144 572
Fragments arranged by size:
2144 572 558 397 344 335 290 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 4721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 1462 1537 713
Size: 749 75 3897
Fragments arranged by size:
3897 749 75
Bpu10I CC'TnA_GC
Cuts at: 1340 1488 1340
Size: 148 4573
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1897 2776 3490 3788 1897
Size: 879 714 298 2830
Fragments arranged by size:
2830 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 106 861 1365 4425 106
Size: 755 504 3060 402
Fragments arranged by size:
3060 755 504 402
BsaAI yAC'GTr
Cuts at: 2074 2074
Size: 4721
BsaBI GATnn'nnATC
Cuts at: 6 1261 2235 4272 6
Size: 1255 974 2037 455
Fragments arranged by size:
2037 1255 974 455
BsaHI Gr'CG_yC
Cuts at: 2152 2400 3102 4598 2152
Size: 248 702 1496 2275
Fragments arranged by size:
2275 1496 702 248
BsaWI w'CCGG_w
Cuts at: 221 419 985 1163 1910 2431 3109 3520 3667
Size: 198 566 178 747 521 678 411 147
Cuts at: 3667 3941 221
Size: 274 1001
Fragments arranged by size:
1001 747 678 566 521 411 274 198
178 147
BseYI C'CCAG_C
Cuts at: 926 2146 2904 3569 3977 926
Size: 1220 758 665 408 1670
Fragments arranged by size:
1670 1220 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2263 2309 3539 104
Size: 2159 46 1230 1286
Fragments arranged by size:
2159 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 328 1607 1735 2513 2703 3563 328
Size: 1279 128 778 190 860 1486
Fragments arranged by size:
1486 1279 860 778 190 128
BsmI GAATG_Cn'
Cuts at: 212 798 212
Size: 586 4135
BsmBI CGTCTCn'nnnn_
Cuts at: 183 183
Size: 4721
Bsp1286I G_dGCh'C
Cuts at: 127 328 552 678 807 933 1215 1607 1735
Size: 201 224 126 129 126 282 392 128
Cuts at: 1735 2346 2439 2513 2703 2765 3563 127
Size: 611 93 74 190 62 798 1285
Fragments arranged by size:
1285 798 611 392 282 224 201 190
129 128 126 126 93 74 62
BspEI T'CCGG_A
Cuts at: 221 419 985 1163 221
Size: 198 566 178 3779
Fragments arranged by size:
3779 566 198 178
BspHI T'CATG_A
Cuts at: 3153 3153
Size: 4721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 1771 1985 2287 2668 401
Size: 1370 214 302 381 2454
Fragments arranged by size:
2454 1370 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3013 3067 4551 4716 50
Size: 2963 54 1484 165 55
Fragments arranged by size:
2963 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2633 2633
Size: 4721
BsrFI r'CCGG_y
Cuts at: 2719 2900 2719
Size: 181 4540
BsrGI T'GTAC_A
Cuts at: 523 523
Size: 4721
BssSI C'ACGA_G
Cuts at: 1561 2992 3700 1561
Size: 1431 708 2582
Fragments arranged by size:
2582 1431 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2098 2098
Size: 4721
BstBI TT'CG_AA
Cuts at: 1411 3082 1411
Size: 1671 3050
BstYI r'GATC_y
Cuts at: 1 965 1574 1746 2213 2236 2571 2817 3123
Size: 964 609 172 467 23 335 246 306
Cuts at: 3123 3135 3221 4273 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 964 609 467 449 335 306 246
172 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1688 1688
Size: 4721
BtgI C'CryG_G
Cuts at: 107 116 1058 1697 107
Size: 9 942 639 3131
Fragments arranged by size:
3131 942 639 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2671 2852 3015 2671
Size: 181 163 4377
Fragments arranged by size:
4377 181 163
BtsI GCAGTG_nn'
Cuts at: 1488 1964 1488
Size: 476 4245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 464 499 1464 1499 464
Size: 35 965 35 3686
Fragments arranged by size:
3686 965 35 35
DraI TTT'AAA
Cuts at: 252 1720 252
Size: 1468 3253
DrdI GACnn_nn'nnGTC
Cuts at: 988 2427 3771 988
Size: 1439 1344 1938
Fragments arranged by size:
1938 1439 1344
EaeI y'GGCC_r
Cuts at: 2306 2480 2871 2898 4243 2306
Size: 174 391 27 1345 2784
Fragments arranged by size:
2784 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2306 2306
Size: 4721
EarI CTCTTCn'nnn_
Cuts at: 145 192 200 502 895 2744 2954 145
Size: 47 8 302 393 1849 210 1912
Fragments arranged by size:
1912 1849 393 302 210 47 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2929 3671 3817 4531 2929
Size: 742 146 714 3119
Fragments arranged by size:
3119 742 714 146
EcoICRI GAG'CTC
Cuts at: 326 1733 326
Size: 1407 3314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 526 713 876 1252 1462 1537 2546 2978
Size: 326 187 163 376 210 75 1009 432
Cuts at: 2978 3331 3993 200
Size: 353 662 928
Fragments arranged by size:
1009 928 662 432 376 353 326 210
187 163 75
EcoNI CCTnn'n_nnAGG
Cuts at: 1516 1516
Size: 4721
EcoO109I rG'GnC_Cy
Cuts at: 804 930 1692 1860 804
Size: 126 762 168 3665
Fragments arranged by size:
3665 762 168 126
EcoRI G'AATT_C
Cuts at: 1725 1725
Size: 4721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 4721
FspI TGC'GCA
Cuts at: 2502 2502
Size: 4721
HaeII r_GCGC'y
Cuts at: 2155 2403 3633 4404 2155
Size: 248 1230 771 2472
Fragments arranged by size:
2472 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 704 736 2563 2593 2595 2625 704
Size: 32 1827 30 2 30 2800
Fragments arranged by size:
2800 1827 32 30 30 2
HincII GTy'rAC
Cuts at: 752 1760 752
Size: 1008 3713
HindIII A'AGCT_T
Cuts at: 1172 1172
Size: 4721
HpaI GTT'AAC
Cuts at: 752 752
Size: 4721
KasI G'GCGC_C
Cuts at: 2151 2399 2151
Size: 248 4473
KpnI G_GTAC'C
Cuts at: 1356 1741 1356
Size: 385 4336
MluI A'CGCG_T
Cuts at: 4154 4154
Size: 4721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3480 3664 3480
Size: 184 4537
MscI TGG'CCA
Cuts at: 2482 2482
Size: 4721
MslI CAynn'nnrTG
Cuts at: 2710 2837 2710
Size: 127 4594
MspA1I CmG'CkG
Cuts at: 1832 2146 2506 3290 3535 1832
Size: 314 360 784 245 3018
Fragments arranged by size:
3018 784 360 314 245
NaeI GCC'GGC
Cuts at: 2902 2902
Size: 4721
NarI GG'CG_CC
Cuts at: 2152 2400 2152
Size: 248 4473
NcoI C'CATG_G
Cuts at: 107 116 1697 107
Size: 9 1581 3131
Fragments arranged by size:
3131 1581 9
NgoMIV G'CCGG_C
Cuts at: 2900 2900
Size: 4721
NspI r_CATG'y
Cuts at: 1774 2274 3877 1774
Size: 500 1603 2618
Fragments arranged by size:
2618 1603 500
PasI CC'CwG_GG
Cuts at: 127 127
Size: 4721
PciI A'CATG_T
Cuts at: 3873 3873
Size: 4721
PmeI GTTT'AAAC
Cuts at: 1720 1720
Size: 4721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 4689
PshAI GACnn'nnGTC
Cuts at: 1096 1096
Size: 4721
PspOMI G'GGCC_C
Cuts at: 803 929 803
Size: 126 4595
PstI C_TGCA'G
Cuts at: 398 1768 2453 398
Size: 1370 685 2666
Fragments arranged by size:
2666 1370 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4721
PvuII CAG'CTG
Cuts at: 2146 2506 2146
Size: 360 4361
RsrII CG'GwC_CG
Cuts at: 2916 2916
Size: 4721
SacI G_AGCT'C
Cuts at: 328 1735 328
Size: 1407 3314
SalI G'TCGA_C
Cuts at: 1758 1758
Size: 4721
SapI GCTCTTCn'nnn_
Cuts at: 145 2744 2954 145
Size: 2599 210 1912
Fragments arranged by size:
2599 1912 210
SbfI CC_TGCA'GG
Cuts at: 1768 1768
Size: 4721
ScaI AGT'ACT
Cuts at: 4238 4238
Size: 4721
SexAI A'CCwGG_T
Cuts at: 1355 1355
Size: 4721
SfcI C'TryA_G
Cuts at: 33 394 1764 2449 3417 3608 33
Size: 361 1370 685 968 191 1146
Fragments arranged by size:
1370 1146 968 685 361 191
SfoI GGC'GCC
Cuts at: 2153 2401 2153
Size: 248 4473
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
SmaI CCC'GGG
Cuts at: 1743 1743
Size: 4721
SmlI C'TyrA_G
Cuts at: 1876 2791 3505 3767 1876
Size: 915 714 262 2830
Fragments arranged by size:
2830 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4721
SphI G_CATG'C
Cuts at: 1774 2274 1774
Size: 500 4221
SspI AAT'ATT
Cuts at: 561 1194 561
Size: 633 4088
StuI AGG'CCT
Cuts at: 1588 1588
Size: 4721
StyI C'CwwG_G
Cuts at: 107 116 1151 1697 1855 2209 107
Size: 9 1035 546 158 354 2619
Fragments arranged by size:
2619 1035 546 354 158 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2304 2437 2896 3105 2304
Size: 133 459 209 3920
Fragments arranged by size:
3920 459 209 133
TatI w'GTAC_w
Cuts at: 523 873 4236 523
Size: 350 3363 1008
Fragments arranged by size:
3363 1008 350
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 401 433 4238 4270 401
Size: 32 3805 32 852
Fragments arranged by size:
3805 852 32 32
Tth111I GACn'n_nGTC
Cuts at: 2518 2518
Size: 4721
XbaI T'CTAG_A
Cuts at: 1752 1752
Size: 4721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2216 114
Size: 2102 2619
XmaI C'CCGG_G
Cuts at: 1741 1741
Size: 4721
XmnI GAAnn'nnTTC
Cuts at: 1265 1588 1265
Size: 323 4398
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BsaXI BseRI BsgI BsiWI BssHII BstEII BstXI BstZ17I
ClaI DraIII EcoRV FseI FspAI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI