Restriction map for
pF1KB5666
(Circular) (Six-base) MAPSORT of: pF1KB5666.seq Check: 2691 from: 1 to: 6014
pF1KB5666 6014 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 451 1676 451
Size: 1225 4789
AatII G_ACGT'C
Cuts at: 464 464
Size: 6014
AccI GT'mk_AC
Cuts at: 2495 3052 2495
Size: 557 5457
Acc65I G'GTAC_C
Cuts at: 2636 3030 2636
Size: 394 5620
AclI AA'CG_TT
Cuts at: 5854 5854
Size: 6014
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1926 2289 2293 3839 4271 4624 5286 1926
Size: 363 4 1546 432 353 662 2654
Fragments arranged by size:
2654 1546 662 432 363 353 4
AflIII A'CryG_T
Cuts at: 1853 3366 5166 5447 1853
Size: 1513 1800 281 2420
Fragments arranged by size:
2420 1800 1513 281
AgeI A'CCGG_T
Cuts at: 1688 1688
Size: 6014
AhdI GACnn_n'nnGTC
Cuts at: 1221 1221
Size: 6014
AleI CACnn'nnGTG
Cuts at: 1711 1711
Size: 6014
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 192 192
Size: 6014
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 634 666 1675 1707 634
Size: 32 1009 32 4941
Fragments arranged by size:
4941 1009 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1605 2859 3319 4757 1605
Size: 1254 460 1438 2862
Fragments arranged by size:
2862 1438 1254 460
ApaI G_GGCC'C
Cuts at: 1020 1075 2916 1020
Size: 55 1841 4118
Fragments arranged by size:
4118 1841 55
ApaLI G'TGCA_C
Cuts at: 177 598 4852 177
Size: 421 4254 1339
Fragments arranged by size:
4254 1339 421
ApoI r'AATT_y
Cuts at: 1843 3018 5367 5623 1843
Size: 1175 2349 256 2234
Fragments arranged by size:
2349 2234 1175 256
AseI AT'TA_AT
Cuts at: 20 2093 20
Size: 2073 3941
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6014
AvaI C'yCGr_G
Cuts at: 2909 3034 2909
Size: 125 5889
BamHI G'GATC_C
Cuts at: 152 2624 3039 152
Size: 2472 415 3127
Fragments arranged by size:
3127 2472 415
BanI G'GyrC_C
Cuts at: 1162 1546 2636 2905 3030 3444 3692 3727 1162
Size: 384 1090 269 125 414 248 35 3449
Fragments arranged by size:
3449 1090 414 384 269 248 125 35
BanII G_rGCy'C
Cuts at: 424 581 613 1020 1075 1388 1929 2916 3028
Size: 157 32 407 55 313 541 987 112
Cuts at: 3028 4058 424
Size: 1030 2380
Fragments arranged by size:
2380 1030 987 541 407 313 157 112
55 32
BbeI G_GCGC'C
Cuts at: 1166 1550 3448 3696 1166
Size: 384 1898 248 3484
Fragments arranged by size:
3484 1898 384 248
BbsI GAAGACnn'nnnn_
Cuts at: 893 2941 893
Size: 2048 3966
BbvCI CC'TCA_GC
Cuts at: 267 499 267
Size: 232 5782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 168 202 2353 2387 2490 2524 168
Size: 34 2151 34 103 34 3658
Fragments arranged by size:
3658 2151 103 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1764 3912 4968 6014 1764
Size: 2148 1056 1046 1764
Fragments arranged by size:
2148 1764 1056 1046
BclI T'GATC_A
Cuts at: 3534 3534
Size: 6014
BglI GCCn_nnn'nGGC
Cuts at: 796 1036 796
Size: 240 5774
BglII A'GATC_T
Cuts at: 3529 5566 3529
Size: 2037 3977
BlpI GC'TnA_GC
Cuts at: 3126 3126
Size: 6014
Bme1580I G_kGCm'C
Cuts at: 181 512 602 1020 1075 1868 2085 2121 2916
Size: 331 90 418 55 793 217 36 795
Cuts at: 2916 3639 3732 4856 181
Size: 723 93 1124 1339
Fragments arranged by size:
1339 1124 795 793 723 418 331 217
93 90 55 36
BmrI ACTGGGnnnn_n'
Cuts at: 1019 1185 3226 3307 3642 5786 1019
Size: 166 2041 81 335 2144 1247
Fragments arranged by size:
2144 2041 1247 335 166 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2439 4381 2439
Size: 1942 4072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 1215 1554 2043 2346 2881 2938 2950 2968
Size: 807 339 489 303 535 57 12 18
Cuts at: 2968 408
Size: 3454
Fragments arranged by size:
3454 807 535 489 339 303 57 18
12
Bpu10I CC'TnA_GC
Cuts at: 267 499 2203 2212 2641 2901 267
Size: 232 1704 9 429 260 3380
Fragments arranged by size:
3380 1704 429 260 232 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 3190 4069 4783 5081 589
Size: 2601 879 714 298 1522
Fragments arranged by size:
2601 1522 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 369 2045 2427 5718 369
Size: 1676 382 3291 665
Fragments arranged by size:
3291 1676 665 382
BsaAI yAC'GTr
Cuts at: 938 975 1356 1854 3367 938
Size: 37 381 498 1513 3585
Fragments arranged by size:
3585 1513 498 381 37
BsaBI GATnn'nnATC
Cuts at: 6 1009 3528 5565 6
Size: 1003 2519 2037 455
Fragments arranged by size:
2519 2037 1003 455
BsaHI Gr'CG_yC
Cuts at: 461 1163 1526 1547 1609 2099 2681 3445 3693
Size: 702 363 21 62 490 582 764 248
Cuts at: 3693 4395 5891 461
Size: 702 1496 584
Fragments arranged by size:
1496 764 702 702 584 582 490 363
248 62 21
BsaWI w'CCGG_w
Cuts at: 1007 1688 2669 3203 3724 4402 4813 4960 5234
Size: 681 981 534 521 678 411 147 274
Cuts at: 5234 1007
Size: 1787
Fragments arranged by size:
1787 981 681 678 534 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1516 1546 2515 2545 1516
Size: 30 969 30 4985
Fragments arranged by size:
4985 969 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1218 1449 2428 2539 2542 1218
Size: 231 979 111 3 4690
Fragments arranged by size:
4690 979 231 111 3
BseYI C'CCAG_C
Cuts at: 542 868 3439 4197 4862 5270 542
Size: 326 2571 758 665 408 1286
Fragments arranged by size:
2571 1286 758 665 408 326
BsiEI CG_ry'CG
Cuts at: 104 3556 3602 4832 104
Size: 3452 46 1230 1286
Fragments arranged by size:
3452 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 181 602 1802 2984 3028 3806 3996 4856 181
Size: 421 1200 1182 44 778 190 860 1339
Fragments arranged by size:
1339 1200 1182 860 778 421 190 44
Bsp1286I G_dGCh'C
Cuts at: 181 424 512 581 602 613 1020 1075 1388
Size: 243 88 69 21 11 407 55 313
Cuts at: 1388 1802 1868 1929 2085 2121 2916 2984 3028
Size: 414 66 61 156 36 795 68 44
Cuts at: 3028 3639 3732 3806 3996 4058 4856 181
Size: 611 93 74 190 62 798 1339
Fragments arranged by size:
1339 798 795 611 414 407 313 243
190 156 93 88 74 69 68 66
62 61 55 44 36 21 11
BspEI T'CCGG_A
Cuts at: 1007 2669 1007
Size: 1662 4352
BspHI T'CATG_A
Cuts at: 1269 1724 4446 1269
Size: 455 2722 2837
Fragments arranged by size:
2837 2722 455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 451 1676 2271 2489 3064 3278 3580 3961 451
Size: 1225 595 218 575 214 302 381 2504
Fragments arranged by size:
2504 1225 595 575 381 302 218 214
BsrBI CCG'CTC
Cuts at: 50 1248 2240 4306 4360 5844 6009 50
Size: 1198 992 2066 54 1484 165 55
Fragments arranged by size:
2066 1484 1198 992 165 55 54
BsrDI GCAATG_nn'
Cuts at: 717 1031 1962 3926 717
Size: 314 931 1964 2805
Fragments arranged by size:
2805 1964 931 314
BsrFI r'CCGG_y
Cuts at: 786 1159 1500 1688 4012 4193 786
Size: 373 341 188 2324 181 2607
Fragments arranged by size:
2607 2324 373 341 188 181
BssSI C'ACGA_G
Cuts at: 4285 4993 4285
Size: 708 5306
BstAPI GCAn_nnn'nTGC
Cuts at: 1447 2387 3391 1447
Size: 940 1004 4070
Fragments arranged by size:
4070 1004 940
BstBI TT'CG_AA
Cuts at: 4375 4375
Size: 6014
BstEII G'GTnAC_C
Cuts at: 1185 1302 1185
Size: 117 5897
BstXI CCAn_nnnn'nTGG
Cuts at: 138 300 138
Size: 162 5852
BstYI r'GATC_y
Cuts at: 1 152 1010 2333 2624 3039 3506 3529 3864
Size: 151 858 1323 291 415 467 23 335
Cuts at: 3864 4110 4416 4428 4514 5566 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1323 1052 858 467 449 415 335 306
291 246 151 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1558 1558
Size: 6014
BtgI C'CryG_G
Cuts at: 107 242 1044 1166 107
Size: 135 802 122 4955
Fragments arranged by size:
4955 802 135 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 171 3964 4145 4308 171
Size: 3793 181 163 1877
Fragments arranged by size:
3793 1877 181 163
BtsI GCAGTG_nn'
Cuts at: 1448 2446 2825 3257 1448
Size: 998 379 432 4205
Fragments arranged by size:
4205 998 432 379
DraI TTT'AAA
Cuts at: 3013 3013
Size: 6014
DraIII CAC_nnn'GTG
Cuts at: 516 516
Size: 6014
DrdI GACnn_nn'nnGTC
Cuts at: 843 3720 5064 843
Size: 2877 1344 1793
Fragments arranged by size:
2877 1793 1344
EaeI y'GGCC_r
Cuts at: 110 290 728 788 2060 2111 3599 3773 4164
Size: 180 438 60 1272 51 1488 174 391
Cuts at: 4164 4191 5536 110
Size: 27 1345 588
Fragments arranged by size:
1488 1345 1272 588 438 391 180 174
60 51 27
EagI C'GGCC_G
Cuts at: 3599 3599
Size: 6014
EarI CTCTTCn'nnn_
Cuts at: 1945 2009 2164 2165 2299 4037 4247 1945
Size: 64 155 1 134 1738 210 3712
Fragments arranged by size:
3712 1738 210 155 134 64 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 252 4222 4964 5110 5824 252
Size: 3970 742 146 714 442
Fragments arranged by size:
3970 742 714 442 146
EcoICRI GAG'CTC
Cuts at: 3026 3026
Size: 6014
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 1215 1554 1926 2043 2289 2293 2346 2881
Size: 807 339 372 117 246 4 53 535
Cuts at: 2881 2938 2950 2968 3839 4271 4624 5286 408
Size: 57 12 18 871 432 353 662 1136
Fragments arranged by size:
1136 871 807 662 535 432 372 353
339 246 117 57 53 18 12 4
EcoNI CCTnn'n_nnAGG
Cuts at: 502 619 1582 1672 1792 2812 502
Size: 117 963 90 120 1020 3704
Fragments arranged by size:
3704 1020 963 120 117 90
EcoO109I rG'GnC_Cy
Cuts at: 402 831 865 1072 1602 1784 1965 2127 2913
Size: 429 34 207 530 182 181 162 786
Cuts at: 2913 3153 402
Size: 240 3263
Fragments arranged by size:
3263 786 530 429 240 207 182 181
162 34
EcoRI G'AATT_C
Cuts at: 1843 3018 1843
Size: 1175 4839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4033 4033
Size: 6014
FspI TGC'GCA
Cuts at: 3795 3795
Size: 6014
HaeII r_GCGC'y
Cuts at: 1166 1550 2146 3448 3696 4926 5697 1166
Size: 384 596 1302 248 1230 771 1483
Fragments arranged by size:
1483 1302 1230 771 596 384 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 368 400 1207 1239 1516 1548 2022 2054 2671
Size: 32 807 32 277 32 474 32 617
Cuts at: 2671 2703 3856 3886 3888 3918 368
Size: 32 1153 30 2 30 2464
Fragments arranged by size:
2464 1153 807 617 474 277 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3053 3053
Size: 6014
HindIII A'AGCT_T
Cuts at: 2764 2764
Size: 6014
KasI G'GCGC_C
Cuts at: 1162 1546 3444 3692 1162
Size: 384 1898 248 3484
Fragments arranged by size:
3484 1898 384 248
KpnI G_GTAC'C
Cuts at: 2640 3034 2640
Size: 394 5620
MluI A'CGCG_T
Cuts at: 5447 5447
Size: 6014
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 872 1722 2417 2828 4773 4957 872
Size: 850 695 411 1945 184 1929
Fragments arranged by size:
1945 1929 850 695 411 184
MscI TGG'CCA
Cuts at: 112 730 2113 3775 112
Size: 618 1383 1662 2351
Fragments arranged by size:
2351 1662 1383 618
MslI CAynn'nnrTG
Cuts at: 1711 1765 4003 4130 1711
Size: 54 2238 127 3595
Fragments arranged by size:
3595 2238 127 54
MspA1I CmG'CkG
Cuts at: 1470 1733 2043 2859 3125 3439 3799 4583 4828
Size: 263 310 816 266 314 360 784 245
Cuts at: 4828 1470
Size: 2656
Fragments arranged by size:
2656 816 784 360 314 310 266 263
245
NaeI GCC'GGC
Cuts at: 788 1161 1502 4195 788
Size: 373 341 2693 2607
Fragments arranged by size:
2693 2607 373 341
NarI GG'CG_CC
Cuts at: 1163 1547 3445 3693 1163
Size: 384 1898 248 3484
Fragments arranged by size:
3484 1898 384 248
NcoI C'CATG_G
Cuts at: 107 1166 107
Size: 1059 4955
NgoMIV G'CCGG_C
Cuts at: 786 1159 1500 4193 786
Size: 373 341 2693 2607
Fragments arranged by size:
2693 2607 373 341
NspI r_CATG'y
Cuts at: 855 2187 3067 3567 5170 855
Size: 1332 880 500 1603 1699
Fragments arranged by size:
1699 1603 1332 880 500
PasI CC'CwG_GG
Cuts at: 138 2189 2514 138
Size: 2051 325 3638
Fragments arranged by size:
3638 2051 325
PciI A'CATG_T
Cuts at: 5166 5166
Size: 6014
PflMI CCAn_nnn'nTGG
Cuts at: 1313 2324 1313
Size: 1011 5003
PmeI GTTT'AAAC
Cuts at: 3013 3013
Size: 6014
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4460 4492 4460
Size: 32 5982
PpuMI rG'GwC_Cy
Cuts at: 831 865 1784 1965 2127 831
Size: 34 919 181 162 4718
Fragments arranged by size:
4718 919 181 162 34
PspOMI G'GGCC_C
Cuts at: 1016 1071 2912 1016
Size: 55 1841 4118
Fragments arranged by size:
4118 1841 55
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1630 1662 1630
Size: 32 5982
PstI C_TGCA'G
Cuts at: 753 1673 1745 2048 2611 2855 3061 3746 753
Size: 920 72 303 563 244 206 685 3021
Fragments arranged by size:
3021 920 685 563 303 244 206 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6014
PvuII CAG'CTG
Cuts at: 2043 2859 3439 3799 2043
Size: 816 580 360 4258
Fragments arranged by size:
4258 816 580 360
RsrII CG'GwC_CG
Cuts at: 4209 4209
Size: 6014
SacI G_AGCT'C
Cuts at: 3028 3028
Size: 6014
SalI G'TCGA_C
Cuts at: 3051 3051
Size: 6014
SanDI GG'GwC_CC
Cuts at: 1784 1784
Size: 6014
SapI GCTCTTCn'nnn_
Cuts at: 4037 4247 4037
Size: 210 5804
SbfI CC_TGCA'GG
Cuts at: 1673 3061 1673
Size: 1388 4626
ScaI AGT'ACT
Cuts at: 5531 5531
Size: 6014
SexAI A'CCwGG_T
Cuts at: 590 1298 590
Size: 708 5306
SfcI C'TryA_G
Cuts at: 33 749 1536 1669 1741 2044 2607 2851 3057
Size: 716 787 133 72 303 563 244 206
Cuts at: 3057 3742 4710 4901 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 787 716 685 563 303 244
206 191 133 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 796 796
Size: 6014
SfoI GGC'GCC
Cuts at: 1164 1548 3446 3694 1164
Size: 384 1898 248 3484
Fragments arranged by size:
3484 1898 384 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6014
SgrAI Cr'CCGG_yG
Cuts at: 1159 1159
Size: 6014
SmaI CCC'GGG
Cuts at: 2911 3036 2911
Size: 125 5889
SmlI C'TyrA_G
Cuts at: 604 3169 4084 4798 5060 604
Size: 2565 915 714 262 1558
Fragments arranged by size:
2565 1558 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6014
SphI G_CATG'C
Cuts at: 3067 3567 3067
Size: 500 5514
StyI C'CwwG_G
Cuts at: 107 113 299 827 1166 3148 3502 107
Size: 6 186 528 339 1982 354 2619
Fragments arranged by size:
2619 1982 528 354 339 186 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1357 2946 3597 3730 4189 4398 1357
Size: 1589 651 133 459 209 2973
Fragments arranged by size:
2973 1589 651 459 209 133
TatI w'GTAC_w
Cuts at: 203 698 2180 2357 5529 203
Size: 495 1482 177 3172 688
Fragments arranged by size:
3172 1482 688 495 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 864 896 1456 1488 2515 2547 5531 5563 864
Size: 32 560 32 1027 32 2984 32 1315
Fragments arranged by size:
2984 1315 1027 560 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2743 3811 2743
Size: 1068 4946
XbaI T'CTAG_A
Cuts at: 3045 3045
Size: 6014
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 588 942 3509 588
Size: 354 2567 3093
Fragments arranged by size:
3093 2567 354
XmaI C'CCGG_G
Cuts at: 2909 3034 2909
Size: 125 5889
XmnI GAAnn'nnTTC
Cuts at: 766 766
Size: 6014
ZraI GAC'GTC
Cuts at: 462 462
Size: 6014
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI AvrII BaeI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspXI SacII SnaBI SrfI SspI StuI SwaI XhoI