Restriction map for
pF1KB5656
(Circular) (Six-base) MAPSORT of: pF1KB5656.seq Check: 4990 from: 1 to: 4154
pF1KB5656 4154 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 966 966
Size: 4154
AccI GT'mk_AC
Cuts at: 1192 1192
Size: 4154
Acc65I G'GTAC_C
Cuts at: 1170 1170
Size: 4154
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 627 1979 2411 3158 3426 274
Size: 353 1352 432 747 268 1002
Fragments arranged by size:
1352 1002 747 432 353 268
AfeI AGC'GCT
Cuts at: 559 559
Size: 4154
AflIII A'CryG_T
Cuts at: 1506 2610 3587 1506
Size: 1104 977 2073
Fragments arranged by size:
2073 1104 977
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 4154
AhdI GACnn_n'nnGTC
Cuts at: 487 487
Size: 4154
AleI CACnn'nnGTG
Cuts at: 895 895
Size: 4154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1024 1024
Size: 4154
AlwNI CAG_nnn'CTG
Cuts at: 960 981 1459 3026 960
Size: 21 478 1567 2088
Fragments arranged by size:
2088 1567 478 21
ApaLI G'TGCA_C
Cuts at: 2924 2924
Size: 4154
ApoI r'AATT_y
Cuts at: 256 778 1158 3507 3763 256
Size: 522 380 2349 256 647
Fragments arranged by size:
2349 647 522 380 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 800 1174 800
Size: 374 3780
BamHI G'GATC_C
Cuts at: 1179 1179
Size: 4154
BanI G'GyrC_C
Cuts at: 229 1170 1584 1832 1867 229
Size: 941 414 248 35 2516
Fragments arranged by size:
2516 941 414 248 35
BanII G_rGCy'C
Cuts at: 815 1168 2198 815
Size: 353 1030 2771
Fragments arranged by size:
2771 1030 353
BbeI G_GCGC'C
Cuts at: 233 1588 1836 233
Size: 1355 248 2551
Fragments arranged by size:
2551 1355 248
BbsI GAAGACnn'nnnn_
Cuts at: 164 293 633 164
Size: 129 340 3685
Fragments arranged by size:
3685 340 129
BciVI GTATCCnnnnn_n'
Cuts at: 2052 2813 4154 2052
Size: 761 1341 2052
Fragments arranged by size:
2052 1341 761
BclI T'GATC_A
Cuts at: 714 1674 714
Size: 960 3194
BglI GCCn_nnn'nGGC
Cuts at: 703 703
Size: 4154
BglII A'GATC_T
Cuts at: 1669 3706 1669
Size: 2037 2117
BlpI GC'TnA_GC
Cuts at: 1266 1266
Size: 4154
Bme1580I G_kGCm'C
Cuts at: 1779 1872 2928 1779
Size: 93 1056 3005
Fragments arranged by size:
3005 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 203 1366 1447 1782 3926 203
Size: 1163 81 335 2144 431
Fragments arranged by size:
2144 1163 431 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 288 2521 288
Size: 2233 1921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 982 928
Size: 54 4100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 1330 2209 2701 2999 260
Size: 1070 879 492 298 1415
Fragments arranged by size:
1415 1070 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 474 958 3858 474
Size: 484 2900 770
Fragments arranged by size:
2900 770 484
BsaAI yAC'GTr
Cuts at: 876 1507 876
Size: 631 3523
BsaBI GATnn'nnATC
Cuts at: 6 1668 3705 6
Size: 1662 2037 455
Fragments arranged by size:
2037 1662 455
BsaHI Gr'CG_yC
Cuts at: 230 1585 1833 2535 4031 230
Size: 1355 248 702 1496 353
Fragments arranged by size:
1496 1355 702 353 248
BsaWI w'CCGG_w
Cuts at: 1343 1864 2542 2816 2963 3374 1343
Size: 521 678 274 147 411 2123
Fragments arranged by size:
2123 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 374 862 374
Size: 488 3666
BseYI C'CCAG_C
Cuts at: 1579 2337 2914 3410 1579
Size: 758 577 496 2323
Fragments arranged by size:
2323 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1051
Size: 4154
BsiEI CG_ry'CG
Cuts at: 104 1146 1696 1742 2950 104
Size: 1042 550 46 1208 1308
Fragments arranged by size:
1308 1208 1042 550 46
BsiHKAI G_wGCw'C
Cuts at: 815 1168 1946 2136 2928 815
Size: 353 778 190 792 2041
Fragments arranged by size:
2041 792 778 353 190
BsmI GAATG_Cn'
Cuts at: 861 861
Size: 4154
Bsp1286I G_dGCh'C
Cuts at: 815 1168 1779 1872 1946 2136 2198 2928 815
Size: 353 611 93 74 190 62 730 2041
Fragments arranged by size:
2041 730 611 353 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3374 3374
Size: 4154
BspHI T'CATG_A
Cuts at: 446 711 717 3330 446
Size: 265 6 2613 1270
Fragments arranged by size:
2613 1270 265 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 966 1204 1418 1720 2101 966
Size: 238 214 302 381 3019
Fragments arranged by size:
3019 381 302 238 214
BsrBI CCG'CTC
Cuts at: 50 930 2446 2500 3984 4149 50
Size: 880 1516 54 1484 165 55
Fragments arranged by size:
1516 1484 880 165 55 54
BsrDI GCAATG_nn'
Cuts at: 327 414 528 2066 327
Size: 87 114 1538 2415
Fragments arranged by size:
2415 1538 114 87
BsrFI r'CCGG_y
Cuts at: 2152 2333 2542 2152
Size: 181 209 3764
Fragments arranged by size:
3764 209 181
BssSI C'ACGA_G
Cuts at: 2425 2783 2425
Size: 358 3796
BstAPI GCAn_nnn'nTGC
Cuts at: 1531 1531
Size: 4154
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstYI r'GATC_y
Cuts at: 1 267 1179 1646 1669 2004 2250 3262 3348
Size: 266 912 467 23 335 246 1012 86
Cuts at: 3348 3360 3706 1
Size: 12 346 449
Fragments arranged by size:
1012 912 467 449 346 335 266 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 545 545
Size: 4154
BtgI C'CryG_G
Cuts at: 107 107
Size: 4154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2104 2285 2448 2104
Size: 181 163 3810
Fragments arranged by size:
3810 181 163
BtsI GCAGTG_nn'
Cuts at: 902 1397 902
Size: 495 3659
DraI TTT'AAA
Cuts at: 1153 1153
Size: 4154
DrdI GACnn_nn'nnGTC
Cuts at: 1860 2718 1860
Size: 858 3296
EaeI y'GGCC_r
Cuts at: 1739 1913 2304 2331 3676 1739
Size: 174 391 27 1345 2217
Fragments arranged by size:
2217 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1739 1739
Size: 4154
EarI CTCTTCn'nnn_
Cuts at: 401 2177 2387 401
Size: 1776 210 2168
Fragments arranged by size:
2168 1776 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 805 2362 2672 2818 3964 805
Size: 1557 310 146 1146 995
Fragments arranged by size:
1557 1146 995 310 146
EcoICRI GAG'CTC
Cuts at: 813 1166 813
Size: 353 3801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 627 928 982 1979 2411 3158 3426 274
Size: 353 301 54 997 432 747 268 1002
Fragments arranged by size:
1002 997 747 432 353 301 268 54
EcoNI CCTnn'n_nnAGG
Cuts at: 973 973
Size: 4154
EcoO109I rG'GnC_Cy
Cuts at: 1293 1293
Size: 4154
EcoRI G'AATT_C
Cuts at: 778 1158 778
Size: 380 3774
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 4154
FspI TGC'GCA
Cuts at: 889 1935 889
Size: 1046 3108
FspAI rTGC'GCAy
Cuts at: 889 889
Size: 4154
HaeII r_GCGC'y
Cuts at: 233 561 1588 1836 2858 3837 233
Size: 328 1027 248 1022 979 550
Fragments arranged by size:
1027 1022 979 550 328 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 165 197 473 505 1996 2026 2028 2058 165
Size: 32 276 32 1491 30 2 30 2261
Fragments arranged by size:
2261 1491 276 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1193 1193
Size: 4154
KasI G'GCGC_C
Cuts at: 229 1584 1832 229
Size: 1355 248 2551
Fragments arranged by size:
2551 1355 248
KpnI G_GTAC'C
Cuts at: 1174 1174
Size: 4154
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 857 2825 3009 857
Size: 1968 184 2002
Fragments arranged by size:
2002 1968 184
MscI TGG'CCA
Cuts at: 1915 1915
Size: 4154
MslI CAynn'nnrTG
Cuts at: 338 511 716 895 2143 2270 338
Size: 173 205 179 1248 127 2222
Fragments arranged by size:
2222 1248 205 179 173 127
MspA1I CmG'CkG
Cuts at: 1265 1579 1939 2952 3197 1265
Size: 314 360 1013 245 2222
Fragments arranged by size:
2222 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2335 2335
Size: 4154
NarI GG'CG_CC
Cuts at: 230 1585 1833 230
Size: 1355 248 2551
Fragments arranged by size:
2551 1355 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4154
NgoMIV G'CCGG_C
Cuts at: 2333 2333
Size: 4154
NspI r_CATG'y
Cuts at: 888 1005 1207 1707 2614 888
Size: 117 202 500 907 2428
Fragments arranged by size:
2428 907 500 202 117
PciI A'CATG_T
Cuts at: 2610 2610
Size: 4154
PfoI T'CCnGG_A
Cuts at: 923 923
Size: 4154
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PmlI CAC'GTG
Cuts at: 876 876
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 4122
PstI C_TGCA'G
Cuts at: 1201 1886 1201
Size: 685 3469
PvuI CG_AT'CG
Cuts at: 104 1146 104
Size: 1042 3112
PvuII CAG'CTG
Cuts at: 1579 1939 1579
Size: 360 3794
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 815 1168 815
Size: 353 3801
SalI G'TCGA_C
Cuts at: 1191 1191
Size: 4154
SapI GCTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 3671 3671
Size: 4154
SfcI C'TryA_G
Cuts at: 33 1031 1115 1197 1882 2875 3066 33
Size: 998 84 82 685 993 191 1121
Fragments arranged by size:
1121 998 993 685 191 84 82
SfoI GGC'GCC
Cuts at: 231 1586 1834 231
Size: 1355 248 2551
Fragments arranged by size:
2551 1355 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SmaI CCC'GGG
Cuts at: 1176 1176
Size: 4154
SmlI C'TyrA_G
Cuts at: 275 1309 2224 2716 2978 275
Size: 1034 915 492 262 1451
Fragments arranged by size:
1451 1034 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 888 1005 1207 1707 888
Size: 117 202 500 3335
Fragments arranged by size:
3335 500 202 117
StuI AGG'CCT
Cuts at: 631 631
Size: 4154
StyI C'CwwG_G
Cuts at: 107 167 1288 1642 107
Size: 60 1121 354 2619
Fragments arranged by size:
2619 1121 354 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 221 429 1737 1870 2329 2538 221
Size: 208 1308 133 459 209 1837
Fragments arranged by size:
1837 1308 459 209 208 133
TatI w'GTAC_w
Cuts at: 3669 3669
Size: 4154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 234 266 3671 3703 234
Size: 32 3405 32 685
Fragments arranged by size:
3405 685 32 32
Tth111I GACn'n_nGTC
Cuts at: 1951 1951
Size: 4154
XbaI T'CTAG_A
Cuts at: 1185 1185
Size: 4154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1649 1649
Size: 4154
XmaI C'CCGG_G
Cuts at: 1174 1174
Size: 4154
XmnI GAAnn'nnTTC
Cuts at: 467 467
Size: 4154
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AloI ApaI AscI AvrII BaeI BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI