Restriction map for
pF1KB5648
(Circular) (Six-base) MAPSORT of: pF1KB5648.seq Check: 1120 from: 1 to: 5591
pF1KB5648 5591 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 840 2448 2629 840
Size: 1608 181 3802
Fragments arranged by size:
3802 1608 181
Acc65I G'GTAC_C
Cuts at: 2607 2607
Size: 5591
AclI AA'CG_TT
Cuts at: 2585 5431 2585
Size: 2846 2745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 1190 1751 3416 3848 4201 4863 230
Size: 960 561 1665 432 353 662 958
Fragments arranged by size:
1665 960 958 662 561 432 353
AflII C'TTAA_G
Cuts at: 1276 1276
Size: 5591
AflIII A'CryG_T
Cuts at: 2174 2367 2943 4743 5024 2174
Size: 193 576 1800 281 2741
Fragments arranged by size:
2741 1800 576 281 193
AgeI A'CCGG_T
Cuts at: 1194 1194
Size: 5591
AhdI GACnn_n'nnGTC
Cuts at: 1604 1604
Size: 5591
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2193 2225 2193
Size: 32 5559
AlwNI CAG_nnn'CTG
Cuts at: 1318 1366 2212 2896 4334 1318
Size: 48 846 684 1438 2575
Fragments arranged by size:
2575 1438 846 684 48
ApaLI G'TGCA_C
Cuts at: 4429 4429
Size: 5591
ApoI r'AATT_y
Cuts at: 809 859 1092 1446 1696 2595 4944 5200 809
Size: 50 233 354 250 899 2349 256 1200
Fragments arranged by size:
2349 1200 899 354 256 250 233 50
AseI AT'TA_AT
Cuts at: 20 20
Size: 5591
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5591
AvaI C'yCGr_G
Cuts at: 173 2611 173
Size: 2438 3153
BamHI G'GATC_C
Cuts at: 2616 2616
Size: 5591
BanI G'GyrC_C
Cuts at: 115 2607 3021 3269 3304 115
Size: 2492 414 248 35 2402
Fragments arranged by size:
2492 2402 414 248 35
BanII G_rGCy'C
Cuts at: 2605 3635 2605
Size: 1030 4561
BbeI G_GCGC'C
Cuts at: 119 3025 3273 119
Size: 2906 248 2437
Fragments arranged by size:
2906 2437 248
BbsI GAAGACnn'nnnn_
Cuts at: 1739 2012 1739
Size: 273 5318
BciVI GTATCCnnnnn_n'
Cuts at: 3489 4545 5591 3489
Size: 1056 1046 3489
Fragments arranged by size:
3489 1056 1046
BclI T'GATC_A
Cuts at: 960 3111 960
Size: 2151 3440
BglII A'GATC_T
Cuts at: 187 3106 5143 187
Size: 2919 2037 635
Fragments arranged by size:
2919 2037 635
BlpI GC'TnA_GC
Cuts at: 2703 2703
Size: 5591
Bme1580I G_kGCm'C
Cuts at: 3216 3309 4433 3216
Size: 93 1124 4374
Fragments arranged by size:
4374 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1649 2803 2884 3219 5363 1649
Size: 1154 81 335 2144 1877
Fragments arranged by size:
2144 1877 1154 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3958 3958
Size: 5591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1340 1340
Size: 5591
Bpu10I CC'TnA_GC
Cuts at: 1640 1640
Size: 5591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1049 1402 2767 3646 4360 4658 1049
Size: 353 1365 879 714 298 1982
Fragments arranged by size:
1982 1365 879 714 353 298
BsaI GGTCTCn'nnnn_
Cuts at: 5295 5295
Size: 5591
BsaAI yAC'GTr
Cuts at: 705 2944 705
Size: 2239 3352
BsaBI GATnn'nnATC
Cuts at: 6 2382 3105 5142 6
Size: 2376 723 2037 455
Fragments arranged by size:
2376 2037 723 455
BsaHI Gr'CG_yC
Cuts at: 116 3022 3270 3972 5468 116
Size: 2906 248 702 1496 239
Fragments arranged by size:
2906 1496 702 248 239
BsaWI w'CCGG_w
Cuts at: 1194 2780 3301 3979 4390 4537 4811 1194
Size: 1586 521 678 411 147 274 1974
Fragments arranged by size:
1974 1586 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1311 2454 3016 3774 4439 4847 1311
Size: 1143 562 758 665 408 2055
Fragments arranged by size:
2055 1143 758 665 562 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 672 1496 672
Size: 824 4767
BsiEI CG_ry'CG
Cuts at: 104 3133 3179 4409 104
Size: 3029 46 1230 1286
Fragments arranged by size:
3029 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2605 3383 3573 4433 2605
Size: 778 190 860 3763
Fragments arranged by size:
3763 860 778 190
BsmI GAATG_Cn'
Cuts at: 2256 2256
Size: 5591
Bsp1286I G_dGCh'C
Cuts at: 2605 3216 3309 3383 3573 3635 4433 2605
Size: 611 93 74 190 62 798 3763
Fragments arranged by size:
3763 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 981 4023 981
Size: 3042 2549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 769 2641 2855 3157 3538 769
Size: 1872 214 302 381 2822
Fragments arranged by size:
2822 1872 381 302 214
BsrBI CCG'CTC
Cuts at: 50 926 3883 3937 5421 5586 50
Size: 876 2957 54 1484 165 55
Fragments arranged by size:
2957 1484 876 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1460 3503 1460
Size: 2043 3548
BsrFI r'CCGG_y
Cuts at: 118 1194 3589 3770 118
Size: 1076 2395 181 1939
Fragments arranged by size:
2395 1939 1076 181
BssSI C'ACGA_G
Cuts at: 3862 4570 3862
Size: 708 4883
BstAPI GCAn_nnn'nTGC
Cuts at: 2968 2968
Size: 5591
BstBI TT'CG_AA
Cuts at: 1494 3952 1494
Size: 2458 3133
BstYI r'GATC_y
Cuts at: 1 187 2616 3083 3106 3441 3687 3993 4005
Size: 186 2429 467 23 335 246 306 12
Cuts at: 4005 4091 5143 1
Size: 86 1052 449
Fragments arranged by size:
2429 1052 467 449 335 306 246 186
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2213 2213
Size: 5591
BtgI C'CryG_G
Cuts at: 107 107
Size: 5591
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3541 3722 3885 3541
Size: 181 163 5247
Fragments arranged by size:
5247 181 163
BtsI GCAGTG_nn'
Cuts at: 684 1958 2834 684
Size: 1274 876 3441
Fragments arranged by size:
3441 1274 876
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1023 1058 1023
Size: 35 5556
DraI TTT'AAA
Cuts at: 2590 2590
Size: 5591
DraIII CAC_nnn'GTG
Cuts at: 501 501
Size: 5591
DrdI GACnn_nn'nnGTC
Cuts at: 3297 4641 3297
Size: 1344 4247
EaeI y'GGCC_r
Cuts at: 3176 3350 3741 3768 5113 3176
Size: 174 391 27 1345 3654
Fragments arranged by size:
3654 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3176 3176
Size: 5591
EarI CTCTTCn'nnn_
Cuts at: 3614 3824 3614
Size: 210 5381
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3799 4541 4687 5401 3799
Size: 742 146 714 3989
Fragments arranged by size:
3989 742 714 146
EcoICRI GAG'CTC
Cuts at: 2603 2603
Size: 5591
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 1190 1340 1751 3416 3848 4201 4863 230
Size: 960 150 411 1665 432 353 662 958
Fragments arranged by size:
1665 960 958 662 432 411 353 150
EcoNI CCTnn'n_nnAGG
Cuts at: 2525 2545 2525
Size: 20 5571
EcoO109I rG'GnC_Cy
Cuts at: 2730 2730
Size: 5591
EcoRI G'AATT_C
Cuts at: 1092 1696 2595 1092
Size: 604 899 4088
Fragments arranged by size:
4088 899 604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3610 3610
Size: 5591
FspI TGC'GCA
Cuts at: 3372 3372
Size: 5591
HaeII r_GCGC'y
Cuts at: 119 3025 3273 4503 5274 119
Size: 2906 248 1230 771 436
Fragments arranged by size:
2906 1230 771 436 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 249 262 281 294 960 992 1313 1345 1578
Size: 13 19 13 666 32 321 32 233
Cuts at: 1578 1590 1610 1622 1779 1811 1831 1863 2013
Size: 12 20 12 157 32 20 32 150
Cuts at: 2013 2045 3433 3463 3465 3495 249
Size: 32 1388 30 2 30 2345
Fragments arranged by size:
2345 1388 666 321 233 157 150 32
32 32 32 32 30 30 20 20
19 13 13 12 12 2
HincII GTy'rAC
Cuts at: 841 2583 2630 841
Size: 1742 47 3802
Fragments arranged by size:
3802 1742 47
HindIII A'AGCT_T
Cuts at: 603 603
Size: 5591
HpaI GTT'AAC
Cuts at: 2583 2583
Size: 5591
KasI G'GCGC_C
Cuts at: 115 3021 3269 115
Size: 2906 248 2437
Fragments arranged by size:
2906 2437 248
KpnI G_GTAC'C
Cuts at: 2611 2611
Size: 5591
MluI A'CGCG_T
Cuts at: 5024 5024
Size: 5591
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1685 2080 4350 4534 1685
Size: 395 2270 184 2742
Fragments arranged by size:
2742 2270 395 184
MscI TGG'CCA
Cuts at: 3352 3352
Size: 5591
MslI CAynn'nnrTG
Cuts at: 521 560 707 3580 3707 521
Size: 39 147 2873 127 2405
Fragments arranged by size:
2873 2405 147 127 39
MspA1I CmG'CkG
Cuts at: 1318 1474 2490 2702 3016 3376 4160 4405 1318
Size: 156 1016 212 314 360 784 245 2504
Fragments arranged by size:
2504 1016 784 360 314 245 212 156
NaeI GCC'GGC
Cuts at: 120 3772 120
Size: 3652 1939
NarI GG'CG_CC
Cuts at: 116 3022 3270 116
Size: 2906 248 2437
Fragments arranged by size:
2906 2437 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5591
NgoMIV G'CCGG_C
Cuts at: 118 3770 118
Size: 3652 1939
NspI r_CATG'y
Cuts at: 2447 2644 3144 4747 2447
Size: 197 500 1603 3291
Fragments arranged by size:
3291 1603 500 197
PciI A'CATG_T
Cuts at: 4743 4743
Size: 5591
PflMI CCAn_nnn'nTGG
Cuts at: 1318 1318
Size: 5591
PmeI GTTT'AAAC
Cuts at: 2590 2590
Size: 5591
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 4037
Size: 32 5559
PstI C_TGCA'G
Cuts at: 2549 2638 3323 2549
Size: 89 685 4817
Fragments arranged by size:
4817 685 89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5591
PvuII CAG'CTG
Cuts at: 2490 3016 3376 2490
Size: 526 360 4705
Fragments arranged by size:
4705 526 360
RsrII CG'GwC_CG
Cuts at: 3786 3786
Size: 5591
SacI G_AGCT'C
Cuts at: 2605 2605
Size: 5591
SalI G'TCGA_C
Cuts at: 839 2628 839
Size: 1789 3802
SapI GCTCTTCn'nnn_
Cuts at: 3614 3824 3614
Size: 210 5381
SbfI CC_TGCA'GG
Cuts at: 2638 2638
Size: 5591
ScaI AGT'ACT
Cuts at: 5108 5108
Size: 5591
SfcI C'TryA_G
Cuts at: 33 2204 2545 2634 3319 4287 4478 33
Size: 2171 341 89 685 968 191 1146
Fragments arranged by size:
2171 1146 968 685 341 191 89
SfoI GGC'GCC
Cuts at: 117 3023 3271 117
Size: 2906 248 2437
Fragments arranged by size:
2906 2437 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5591
SgrAI Cr'CCGG_yG
Cuts at: 118 118
Size: 5591
SmaI CCC'GGG
Cuts at: 2613 2613
Size: 5591
SmlI C'TyrA_G
Cuts at: 173 1064 1276 1381 2746 3661 4375 4637 173
Size: 891 212 105 1365 915 714 262 1127
Fragments arranged by size:
1365 1127 915 891 714 262 212 105
SpeI A'CTAG_T
Cuts at: 67 2062 67
Size: 1995 3596
SphI G_CATG'C
Cuts at: 2644 3144 2644
Size: 500 5091
SspI AAT'ATT
Cuts at: 804 940 1663 2018 804
Size: 136 723 355 4377
Fragments arranged by size:
4377 723 355 136
StuI AGG'CCT
Cuts at: 277 277
Size: 5591
StyI C'CwwG_G
Cuts at: 107 392 2725 3079 107
Size: 285 2333 354 2619
Fragments arranged by size:
2619 2333 354 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 809 1437 3174 3307 3766 3975 809
Size: 628 1737 133 459 209 2425
Fragments arranged by size:
2425 1737 628 459 209 133
TatI w'GTAC_w
Cuts at: 1019 5106 1019
Size: 4087 1504
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 485 517 5108 5140 485
Size: 32 4591 32 936
Fragments arranged by size:
4591 936 32 32
Tth111I GACn'n_nGTC
Cuts at: 3388 3388
Size: 5591
XbaI T'CTAG_A
Cuts at: 2622 2622
Size: 5591
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 733 3086 733
Size: 2353 3238
XhoI C'TCGA_G
Cuts at: 173 173
Size: 5591
XmaI C'CCGG_G
Cuts at: 2611 2611
Size: 5591
XmnI GAAnn'nnTTC
Cuts at: 785 785
Size: 5591
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I ClaI EcoRV FseI FspAI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SwaI ZraI