Restriction map for
pF1KB5647
(Circular) (Six-base) MAPSORT of: pF1KB5647.seq Check: 9490 from: 1 to: 4064
pF1KB5647 4064 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1102 1102
Size: 4064
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 549 826 1889 2321 3068 3336 549
Size: 277 1063 432 747 268 1277
Fragments arranged by size:
1277 1063 747 432 277 268
AflIII A'CryG_T
Cuts at: 1416 2520 3497 1416
Size: 1104 977 1983
Fragments arranged by size:
1983 1104 977
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AhdI GACnn_n'nnGTC
Cuts at: 706 706
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 1369 2936 1369
Size: 1567 2497
ApaI G_GGCC'C
Cuts at: 697 697
Size: 4064
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 445 831 937 1068 3417 3673 445
Size: 386 106 131 2349 256 836
Fragments arranged by size:
2349 836 386 256 131 106
AseI AT'TA_AT
Cuts at: 20 1022 20
Size: 1002 3062
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 281 1084 281
Size: 803 3261
BamHI G'GATC_C
Cuts at: 189 1089 189
Size: 900 3164
BanI G'GyrC_C
Cuts at: 169 1080 1494 1742 1777 169
Size: 911 414 248 35 2456
Fragments arranged by size:
2456 911 414 248 35
BanII G_rGCy'C
Cuts at: 697 998 1078 2108 697
Size: 301 80 1030 2653
Fragments arranged by size:
2653 1030 301 80
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 1584 1584
Size: 4064
BglI GCCn_nnn'nGGC
Cuts at: 292 292
Size: 4064
BglII A'GATC_T
Cuts at: 1579 3616 1579
Size: 2037 2027
BlpI GC'TnA_GC
Cuts at: 655 997 1176 655
Size: 342 179 3543
Fragments arranged by size:
3543 342 179
Bme1580I G_kGCm'C
Cuts at: 697 1689 1782 2838 697
Size: 992 93 1056 1923
Fragments arranged by size:
1923 1056 992 93
BmrI ACTGGGnnnn_n'
Cuts at: 1276 1357 1692 3836 1276
Size: 81 335 2144 1504
Fragments arranged by size:
2144 1504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 991
Size: 4064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2119 2611 2909 1240
Size: 879 492 298 2395
Fragments arranged by size:
2395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3768 3768
Size: 4064
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 1578 3615 6
Size: 1572 2037 455
Fragments arranged by size:
2037 1572 455
BsaHI Gr'CG_yC
Cuts at: 1495 1743 2445 3941 1495
Size: 248 702 1496 1618
Fragments arranged by size:
1618 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2726 2873 3284 1253
Size: 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 650 1055 1489 2247 2824 3320 650
Size: 405 434 758 577 496 1394
Fragments arranged by size:
1394 758 577 496 434 405
BsiEI CG_ry'CG
Cuts at: 104 1606 1652 2860 104
Size: 1502 46 1208 1308
Fragments arranged by size:
1502 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 998 1078 1856 2046 2838 998
Size: 80 778 190 792 2224
Fragments arranged by size:
2224 792 778 190 80
BsmI GAATG_Cn'
Cuts at: 735 735
Size: 4064
Bsp1286I G_dGCh'C
Cuts at: 697 998 1078 1689 1782 1856 2046 2108 2838
Size: 301 80 611 93 74 190 62 730
Cuts at: 2838 697
Size: 1923
Fragments arranged by size:
1923 730 611 301 190 93 80 74
62
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 3240 3240
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1114 1328 1630 2011 1114
Size: 214 302 381 3167
Fragments arranged by size:
3167 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2356 2410 3894 4059 50
Size: 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1976 1976
Size: 4064
BsrFI r'CCGG_y
Cuts at: 325 2062 2243 2452 325
Size: 1737 181 209 1937
Fragments arranged by size:
1937 1737 209 181
BssSI C'ACGA_G
Cuts at: 2335 2693 2335
Size: 358 3706
BstAPI GCAn_nnn'nTGC
Cuts at: 338 1441 338
Size: 1103 2961
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstYI r'GATC_y
Cuts at: 1 189 252 1089 1556 1579 1914 2160 3172
Size: 188 63 837 467 23 335 246 1012
Cuts at: 3172 3258 3270 3616 1
Size: 86 12 346 449
Fragments arranged by size:
1012 837 467 449 346 335 246 188
86 63 23 12
BtgI C'CryG_G
Cuts at: 689 689
Size: 4064
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2014 2195 2358 2014
Size: 181 163 3720
Fragments arranged by size:
3720 181 163
BtsI GCAGTG_nn'
Cuts at: 276 1307 276
Size: 1031 3033
DraI TTT'AAA
Cuts at: 990 1063 990
Size: 73 3991
DrdI GACnn_nn'nnGTC
Cuts at: 1770 2628 1770
Size: 858 3206
EaeI y'GGCC_r
Cuts at: 486 1649 1823 2214 2241 3586 486
Size: 1163 174 391 27 1345 964
Fragments arranged by size:
1345 1163 964 391 174 27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 368 857 2087 2297 368
Size: 489 1230 210 2135
Fragments arranged by size:
2135 1230 489 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2272 2582 2728 3874 2272
Size: 310 146 1146 2462
Fragments arranged by size:
2462 1146 310 146
EcoICRI GAG'CTC
Cuts at: 996 1076 996
Size: 80 3984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 549 826 991 1889 2321 3068 3336 549
Size: 277 165 898 432 747 268 1277
Fragments arranged by size:
1277 898 747 432 277 268 165
EcoO109I rG'GnC_Cy
Cuts at: 694 1203 694
Size: 509 3555
EcoRI G'AATT_C
Cuts at: 937 1068 937
Size: 131 3933
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 851 2083 851
Size: 1232 2832
FspI TGC'GCA
Cuts at: 1845 1845
Size: 4064
HaeII r_GCGC'y
Cuts at: 1498 1746 2768 3747 1498
Size: 248 1022 979 1815
Fragments arranged by size:
1815 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 692 724 1906 1936 1938 1968 692
Size: 32 1182 30 2 30 2788
Fragments arranged by size:
2788 1182 32 30 30 2
HincII GTy'rAC
Cuts at: 756 1103 756
Size: 347 3717
HindIII A'AGCT_T
Cuts at: 744 744
Size: 4064
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MfeI C'AATT_G
Cuts at: 551 551
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2735 2919 2735
Size: 184 3880
MscI TGG'CCA
Cuts at: 488 1825 488
Size: 1337 2727
MslI CAynn'nnrTG
Cuts at: 688 2053 2180 688
Size: 1365 127 2572
Fragments arranged by size:
2572 1365 127
MspA1I CmG'CkG
Cuts at: 186 1175 1489 1849 2862 3107 186
Size: 989 314 360 1013 245 1143
Fragments arranged by size:
1143 1013 989 360 314 245
NaeI GCC'GGC
Cuts at: 327 2245 327
Size: 1918 2146
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NcoI C'CATG_G
Cuts at: 689 689
Size: 4064
NgoMIV G'CCGG_C
Cuts at: 325 2243 325
Size: 1918 2146
NspI r_CATG'y
Cuts at: 223 1117 1617 2524 223
Size: 894 500 907 1763
Fragments arranged by size:
1763 907 894 500
PciI A'CATG_T
Cuts at: 2520 2520
Size: 4064
PflMI CCAn_nnn'nTGG
Cuts at: 640 1041 640
Size: 401 3663
PfoI T'CCnGG_A
Cuts at: 192 192
Size: 4064
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 614 646 3203 3235 614
Size: 32 2557 32 1443
Fragments arranged by size:
2557 1443 32 32
PspOMI G'GGCC_C
Cuts at: 693 693
Size: 4064
PspXI vC'TCGA_Gb
Cuts at: 281 281
Size: 4064
PstI C_TGCA'G
Cuts at: 260 1111 1796 260
Size: 851 685 2528
Fragments arranged by size:
2528 851 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 186 1489 1849 186
Size: 1303 360 2401
Fragments arranged by size:
2401 1303 360
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 998 1078 998
Size: 80 3984
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SfcI C'TryA_G
Cuts at: 33 130 256 1107 1792 2785 2976 33
Size: 97 126 851 685 993 191 1121
Fragments arranged by size:
1121 993 851 685 191 126 97
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 281 1219 2134 2626 2888 281
Size: 938 915 492 262 1457
Fragments arranged by size:
1457 938 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
SspI AAT'ATT
Cuts at: 543 813 873 543
Size: 270 60 3734
Fragments arranged by size:
3734 270 60
StyI C'CwwG_G
Cuts at: 689 1198 1552 689
Size: 509 354 3201
Fragments arranged by size:
3201 509 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1647 1780 2239 2448 1647
Size: 133 459 209 3263
Fragments arranged by size:
3263 459 209 133
TatI w'GTAC_w
Cuts at: 902 974 3579 902
Size: 72 2605 1387
Fragments arranged by size:
2605 1387 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4032
Tth111I GACn'n_nGTC
Cuts at: 1861 1861
Size: 4064
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1559 1559
Size: 4064
XhoI C'TCGA_G
Cuts at: 281 281
Size: 4064
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
XmnI GAAnn'nnTTC
Cuts at: 629 629
Size: 4064
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI ZraI