Restriction map for
pF1KB5642
(Circular) (Six-base) MAPSORT of: pF1KB5642.seq Check: 2844 from: 1 to: 4163
pF1KB5642 4163 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1105 1105
Size: 4163
AatII G_ACGT'C
Cuts at: 374 374
Size: 4163
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 659 1179 659
Size: 520 3643
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1988 2420 2773 3435 1988
Size: 432 353 662 2716
Fragments arranged by size:
2716 662 432 353
AflIII A'CryG_T
Cuts at: 1515 3315 3596 1515
Size: 1800 281 2082
Fragments arranged by size:
2082 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 642 1468 2906 642
Size: 826 1438 1899
Fragments arranged by size:
1899 1438 826
ApaLI G'TGCA_C
Cuts at: 3001 3001
Size: 4163
ApoI r'AATT_y
Cuts at: 1167 3516 3772 1167
Size: 2349 256 1558
Fragments arranged by size:
2349 1558 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 198 508 848 1183 198
Size: 310 340 335 3178
Fragments arranged by size:
3178 340 335 310
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 187 355 423 659 682 919 1179 1593 1841
Size: 168 68 236 23 237 260 414 248
Cuts at: 1841 1876 187
Size: 35 2474
Fragments arranged by size:
2474 414 260 248 237 236 168 68
35 23
BanII G_rGCy'C
Cuts at: 1177 2207 1177
Size: 1030 3133
BbeI G_GCGC'C
Cuts at: 191 359 427 686 1597 1845 191
Size: 168 68 259 911 248 2509
Fragments arranged by size:
2509 911 259 248 168 68
BbsI GAAGACnn'nnnn_
Cuts at: 833 949 833
Size: 116 4047
BciVI GTATCCnnnnn_n'
Cuts at: 2061 3117 4163 2061
Size: 1056 1046 2061
Fragments arranged by size:
2061 1056 1046
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BglI GCCn_nnn'nGGC
Cuts at: 928 928
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 1788 1881 3005 1788
Size: 93 1124 2946
Fragments arranged by size:
2946 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 897 1375 1456 1791 3935 897
Size: 478 81 335 2144 1125
Fragments arranged by size:
2144 1125 478 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 669 2530 669
Size: 1861 2302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 664 757 1145 559
Size: 105 93 388 3577
Fragments arranged by size:
3577 388 105 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 1339 2218 2932 3230 451
Size: 888 879 714 298 1384
Fragments arranged by size:
1384 888 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 733 3867 733
Size: 3134 1029
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 188 356 371 424 458 595 683 1594 1842
Size: 168 15 53 34 137 88 911 248
Cuts at: 1842 2544 4040 188
Size: 702 1496 311
Fragments arranged by size:
1496 911 702 311 248 168 137 88
53 34 15
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2962 3109 3383 1352
Size: 521 678 411 147 274 2132
Fragments arranged by size:
2132 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 610 640 610
Size: 30 4133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 448 493 625 628 631 448
Size: 45 132 3 3 3980
Fragments arranged by size:
3980 132 45 3 3
BseYI C'CCAG_C
Cuts at: 1040 1588 2346 3011 3419 1040
Size: 548 758 665 408 1784
Fragments arranged by size:
1784 758 665 548 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 787
Size: 4163
BsiEI CG_ry'CG
Cuts at: 104 343 452 520 1705 1751 2981 104
Size: 239 109 68 1185 46 1230 1286
Fragments arranged by size:
1286 1230 1185 239 109 68 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1955 2145 3005 1177
Size: 778 190 860 2335
Fragments arranged by size:
2335 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 775 775
Size: 4163
Bsp1286I G_dGCh'C
Cuts at: 1177 1788 1881 1955 2145 2207 3005 1177
Size: 611 93 74 190 62 798 2335
Fragments arranged by size:
2335 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2595 2595
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 698 1105 1213 1427 1729 2110 698
Size: 407 108 214 302 381 2751
Fragments arranged by size:
2751 407 381 302 214 108
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 163 175 265 289 519 632 679 2161 2342
Size: 12 90 24 230 113 47 1482 181
Cuts at: 2342 163
Size: 1984
Fragments arranged by size:
1984 1482 230 181 113 90 47 24
12
BssSI C'ACGA_G
Cuts at: 778 2434 3142 778
Size: 1656 708 1799
Fragments arranged by size:
1799 1656 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1120 1540 1120
Size: 420 3743
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstYI r'GATC_y
Cuts at: 1 1188 1655 1678 2013 2259 2565 2577 2663
Size: 1187 467 23 335 246 306 12 86
Cuts at: 2663 3715 1
Size: 1052 449
Fragments arranged by size:
1187 1052 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2113 2294 2457 2113
Size: 181 163 3819
Fragments arranged by size:
3819 181 163
BtsI GCAGTG_nn'
Cuts at: 1406 1406
Size: 4163
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 3213 1869
Size: 1344 2819
EaeI y'GGCC_r
Cuts at: 340 449 517 1748 1922 2313 2340 3685 340
Size: 109 68 1231 174 391 27 1345 818
Fragments arranged by size:
1345 1231 818 391 174 109 68 27
EagI C'GGCC_G
Cuts at: 340 449 517 1748 340
Size: 109 68 1231 2755
Fragments arranged by size:
2755 1231 109 68
EarI CTCTTCn'nnn_
Cuts at: 470 2186 2396 470
Size: 1716 210 2237
Fragments arranged by size:
2237 1716 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 296 2371 3113 3259 3973 296
Size: 2075 742 146 714 486
Fragments arranged by size:
2075 742 714 486 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 664 757 1145 1988 2420 2773 3435 559
Size: 105 93 388 843 432 353 662 1287
Fragments arranged by size:
1287 843 662 432 388 353 105 93
EcoO109I rG'GnC_Cy
Cuts at: 363 1074 1302 363
Size: 711 228 3224
Fragments arranged by size:
3224 711 228
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 817 2182 817
Size: 1365 2798
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 191 290 359 427 686 736 1597 1845 3075
Size: 99 69 68 259 50 861 248 1230
Cuts at: 3075 3846 191
Size: 771 508
Fragments arranged by size:
1230 861 771 508 259 248 99 69
68 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 131 163 639 671 823 842 855 874 888
Size: 32 476 32 152 19 13 19 14
Cuts at: 888 920 2005 2035 2037 2067 131
Size: 32 1085 30 2 30 2227
Fragments arranged by size:
2227 1085 476 152 32 32 32 30
30 19 19 14 13 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 187 355 423 682 1593 1841 187
Size: 168 68 259 911 248 2509
Fragments arranged by size:
2509 911 259 248 168 68
KpnI G_GTAC'C
Cuts at: 663 1183 663
Size: 520 3643
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2922 3106 2922
Size: 184 3979
MscI TGG'CCA
Cuts at: 1924 1924
Size: 4163
MslI CAynn'nnrTG
Cuts at: 2152 2279 2152
Size: 127 4036
MspA1I CmG'CkG
Cuts at: 182 454 534 1274 1588 1948 2732 2977 182
Size: 272 80 740 314 360 784 245 1368
Fragments arranged by size:
1368 784 740 360 314 272 245 80
NaeI GCC'GGC
Cuts at: 165 177 267 291 521 2344 165
Size: 12 90 24 230 1823 1984
Fragments arranged by size:
1984 1823 230 90 24 12
NarI GG'CG_CC
Cuts at: 188 356 424 683 1594 1842 188
Size: 168 68 259 911 248 2509
Fragments arranged by size:
2509 911 259 248 168 68
NgoMIV G'CCGG_C
Cuts at: 163 175 265 289 519 2342 163
Size: 12 90 24 230 1823 1984
Fragments arranged by size:
1984 1823 230 90 24 12
NotI GC'GGCC_GC
Cuts at: 340 340
Size: 4163
NspI r_CATG'y
Cuts at: 801 1216 1716 3319 801
Size: 415 500 1603 1645
Fragments arranged by size:
1645 1603 500 415
PciI A'CATG_T
Cuts at: 3315 3315
Size: 4163
PfoI T'CCnGG_A
Cuts at: 325 325
Size: 4163
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 4131
PpuMI rG'GwC_Cy
Cuts at: 363 1074 363
Size: 711 3452
PstI C_TGCA'G
Cuts at: 1210 1895 1210
Size: 685 3478
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 1588 1948 1588
Size: 360 3803
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 1206 1891 2859 3050 33
Size: 1173 685 968 191 1146
Fragments arranged by size:
1173 1146 968 685 191
SfoI GGC'GCC
Cuts at: 189 357 425 684 1595 1843 189
Size: 168 68 259 911 248 2509
Fragments arranged by size:
2509 911 259 248 168 68
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SgrAI Cr'CCGG_yG
Cuts at: 289 679 289
Size: 390 3773
SmaI CCC'GGG
Cuts at: 200 1185 200
Size: 985 3178
SmlI C'TyrA_G
Cuts at: 430 848 1318 2233 2947 3209 430
Size: 418 470 915 714 262 1384
Fragments arranged by size:
1384 915 714 470 418 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 801 1216 1716 801
Size: 415 500 3248
Fragments arranged by size:
3248 500 415
StuI AGG'CCT
Cuts at: 232 232
Size: 4163
StyI C'CwwG_G
Cuts at: 686 792 1297 1651 686
Size: 106 505 354 3198
Fragments arranged by size:
3198 505 354 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 1879 2338 2547 1746
Size: 133 459 209 3362
Fragments arranged by size:
3362 459 209 133
TatI w'GTAC_w
Cuts at: 3678 3678
Size: 4163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4131
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1658 1658
Size: 4163
XhoI C'TCGA_G
Cuts at: 848 848
Size: 4163
XmaI C'CCGG_G
Cuts at: 198 1183 198
Size: 985 3178
ZraI GAC'GTC
Cuts at: 372 372
Size: 4163
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI SwaI XmnI