Restriction map for
pF1KB5641
(Circular) (Six-base) MAPSORT of: pF1KB5641.seq Check: 3186 from: 1 to: 5390
pF1KB5641 5390 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 185 2108 185
Size: 1923 3467
AccI GT'mk_AC
Cuts at: 1910 2428 1910
Size: 518 4872
Acc65I G'GTAC_C
Cuts at: 2406 2406
Size: 5390
AclI AA'CG_TT
Cuts at: 898 5230 898
Size: 4332 1058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 782 3215 3647 4000 4662 782
Size: 2433 432 353 662 1510
Fragments arranged by size:
2433 1510 662 432 353
AfeI AGC'GCT
Cuts at: 1781 2146 1781
Size: 365 5025
AflIII A'CryG_T
Cuts at: 1517 2189 2742 4542 4823 1517
Size: 672 553 1800 281 2084
Fragments arranged by size:
2084 1800 672 553 281
AhdI GACnn_n'nnGTC
Cuts at: 1986 1986
Size: 5390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1594 1594
Size: 5390
AlwNI CAG_nnn'CTG
Cuts at: 372 864 1231 2695 4133 372
Size: 492 367 1464 1438 1629
Fragments arranged by size:
1629 1464 1438 492 367
ApaLI G'TGCA_C
Cuts at: 842 4228 842
Size: 3386 2004
ApoI r'AATT_y
Cuts at: 1673 2394 4743 4999 1673
Size: 721 2349 256 2064
Fragments arranged by size:
2349 2064 721 256
AseI AT'TA_AT
Cuts at: 20 1196 20
Size: 1176 4214
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5390
AvaI C'yCGr_G
Cuts at: 543 2410 543
Size: 1867 3523
BamHI G'GATC_C
Cuts at: 354 854 2415 354
Size: 500 1561 3329
Fragments arranged by size:
3329 1561 500
BanI G'GyrC_C
Cuts at: 867 2406 2820 3068 3103 867
Size: 1539 414 248 35 3154
Fragments arranged by size:
3154 1539 414 248 35
BanII G_rGCy'C
Cuts at: 478 527 2404 3434 478
Size: 49 1877 1030 2434
Fragments arranged by size:
2434 1877 1030 49
BbeI G_GCGC'C
Cuts at: 2824 3072 2824
Size: 248 5142
BbsI GAAGACnn'nnnn_
Cuts at: 770 770
Size: 5390
BbvCI CC'TCA_GC
Cuts at: 618 618
Size: 5390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2161 2195 2161
Size: 34 5356
BciVI GTATCCnnnnn_n'
Cuts at: 3288 4344 5390 3288
Size: 1056 1046 3288
Fragments arranged by size:
3288 1056 1046
BclI T'GATC_A
Cuts at: 957 2910 957
Size: 1953 3437
BfrBI ATG'CAT
Cuts at: 1285 1285
Size: 5390
BglII A'GATC_T
Cuts at: 606 1086 1739 2905 4942 606
Size: 480 653 1166 2037 1054
Fragments arranged by size:
2037 1166 1054 653 480
BlpI GC'TnA_GC
Cuts at: 1546 2502 1546
Size: 956 4434
Bme1580I G_kGCm'C
Cuts at: 846 3015 3108 4232 846
Size: 2169 93 1124 2004
Fragments arranged by size:
2169 2004 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 744 1241 2602 2683 3018 5162 744
Size: 497 1361 81 335 2144 972
Fragments arranged by size:
2144 1361 972 497 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1761 3757 1761
Size: 1996 3394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1552 1075
Size: 477 4913
Bpu10I CC'TnA_GC
Cuts at: 472 618 472
Size: 146 5244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2566 3445 4159 4457 2566
Size: 879 714 298 3499
Fragments arranged by size:
3499 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5094 5094
Size: 5390
BsaAI yAC'GTr
Cuts at: 1884 2743 1884
Size: 859 4531
BsaBI GATnn'nnATC
Cuts at: 6 2904 4941 6
Size: 2898 2037 455
Fragments arranged by size:
2898 2037 455
BsaHI Gr'CG_yC
Cuts at: 182 2105 2821 3069 3771 5267 182
Size: 1923 716 248 702 1496 305
Fragments arranged by size:
1923 1496 716 702 305 248
BsaWI w'CCGG_w
Cuts at: 851 2579 3100 3778 4189 4336 4610 851
Size: 1728 521 678 411 147 274 1631
Fragments arranged by size:
1728 1631 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1116 1146 1116
Size: 30 5360
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 895 895
Size: 5390
BseYI C'CCAG_C
Cuts at: 201 375 566 677 922 1080 2815 3573 4238
Size: 174 191 111 245 158 1735 758 665
Cuts at: 4238 4646 201
Size: 408 945
Fragments arranged by size:
1735 945 758 665 408 245 191 174
158 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 832 1564 1906 2224 347
Size: 485 732 342 318 3513
Fragments arranged by size:
3513 732 485 342 318
BsiEI CG_ry'CG
Cuts at: 104 2932 2978 4208 104
Size: 2828 46 1230 1286
Fragments arranged by size:
2828 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 846 2404 3182 3372 4232 846
Size: 1558 778 190 860 2004
Fragments arranged by size:
2004 1558 860 778 190
BsmI GAATG_Cn'
Cuts at: 1776 1776
Size: 5390
BsmBI CGTCTCn'nnnn_
Cuts at: 1077 1077
Size: 5390
Bsp1286I G_dGCh'C
Cuts at: 478 527 846 2404 3015 3108 3182 3372 3434
Size: 49 319 1558 611 93 74 190 62
Cuts at: 3434 4232 478
Size: 798 1636
Fragments arranged by size:
1636 1558 798 611 319 190 93 74
62 49
BspHI T'CATG_A
Cuts at: 3822 3822
Size: 5390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2440 2654 2956 3337 2440
Size: 214 302 381 4493
Fragments arranged by size:
4493 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3682 3736 5220 5385 50
Size: 3632 54 1484 165 55
Fragments arranged by size:
3632 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1013 1718 3302 1013
Size: 705 1584 3101
Fragments arranged by size:
3101 1584 705
BsrFI r'CCGG_y
Cuts at: 3388 3569 3388
Size: 181 5209
BssSI C'ACGA_G
Cuts at: 1735 3661 4369 1735
Size: 1926 708 2756
Fragments arranged by size:
2756 1926 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2767 2767
Size: 5390
BstBI TT'CG_AA
Cuts at: 3751 3751
Size: 5390
BstXI CCAn_nnnn'nTGG
Cuts at: 1755 1782 1755
Size: 27 5363
BstYI r'GATC_y
Cuts at: 1 354 606 854 1086 1739 2415 2882 2905
Size: 353 252 248 232 653 676 467 23
Cuts at: 2905 3240 3486 3792 3804 3890 4942 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 676 653 467 449 353 335 306
252 248 246 232 86 23 12
BtgI C'CryG_G
Cuts at: 341 654 1159 1562 341
Size: 313 505 403 4169
Fragments arranged by size:
4169 505 403 313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 890 3340 3521 3684 890
Size: 2450 181 163 2596
Fragments arranged by size:
2596 2450 181 163
BtsI GCAGTG_nn'
Cuts at: 158 479 844 2633 158
Size: 321 365 1789 2915
Fragments arranged by size:
2915 1789 365 321
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 690 725 690
Size: 35 5355
DraI TTT'AAA
Cuts at: 1253 2389 1253
Size: 1136 4254
DrdI GACnn_nn'nnGTC
Cuts at: 900 1918 3096 4440 900
Size: 1018 1178 1344 1850
Fragments arranged by size:
1850 1344 1178 1018
EaeI y'GGCC_r
Cuts at: 161 338 768 1632 2975 3149 3540 3567 4912
Size: 177 430 864 1343 174 391 27 1345
Cuts at: 4912 161
Size: 639
Fragments arranged by size:
1345 1343 864 639 430 391 177 174
27
EagI C'GGCC_G
Cuts at: 2975 2975
Size: 5390
EarI CTCTTCn'nnn_
Cuts at: 662 1397 2039 2298 3413 3623 662
Size: 735 642 259 1115 210 2429
Fragments arranged by size:
2429 1115 735 642 259 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3598 4340 4486 5200 3598
Size: 742 146 714 3788
Fragments arranged by size:
3788 742 714 146
EcoICRI GAG'CTC
Cuts at: 2402 2402
Size: 5390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 782 1075 1552 3215 3647 4000 4662 782
Size: 293 477 1663 432 353 662 1510
Fragments arranged by size:
1663 1510 662 477 432 353 293
EcoO109I rG'GnC_Cy
Cuts at: 456 1022 1407 2529 456
Size: 566 385 1122 3317
Fragments arranged by size:
3317 1122 566 385
EcoRI G'AATT_C
Cuts at: 2394 2394
Size: 5390
EcoRV GAT'ATC
Cuts at: 2227 2227
Size: 5390
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1129 3409 1129
Size: 2280 3110
FspI TGC'GCA
Cuts at: 3171 3171
Size: 5390
HaeII r_GCGC'y
Cuts at: 1783 2148 2824 3072 4302 5073 1783
Size: 365 676 248 1230 771 2100
Fragments arranged by size:
2100 1230 771 676 365 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 868 900 901 933 1475 1507 1972 2004 3232
Size: 32 1 32 542 32 465 32 1228
Cuts at: 3232 3262 3264 3294 868
Size: 30 2 30 2964
Fragments arranged by size:
2964 1228 542 465 32 32 32 32
30 30 2 1
HincII GTy'rAC
Cuts at: 141 1236 1440 2429 141
Size: 1095 204 989 3102
Fragments arranged by size:
3102 1095 989 204
HpaI GTT'AAC
Cuts at: 1236 1236
Size: 5390
KasI G'GCGC_C
Cuts at: 2820 3068 2820
Size: 248 5142
KpnI G_GTAC'C
Cuts at: 2410 2410
Size: 5390
MluI A'CGCG_T
Cuts at: 4823 4823
Size: 5390
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1873 1902 1994 4149 4333 1873
Size: 29 92 2155 184 2930
Fragments arranged by size:
2930 2155 184 92 29
MscI TGG'CCA
Cuts at: 163 3151 163
Size: 2988 2402
MslI CAynn'nnrTG
Cuts at: 1316 1606 2002 2377 3379 3506 1316
Size: 290 396 375 1002 127 3200
Fragments arranged by size:
3200 1002 396 375 290 127
MspA1I CmG'CkG
Cuts at: 127 170 361 890 2501 2815 3175 3959 4204
Size: 43 191 529 1611 314 360 784 245
Cuts at: 4204 127
Size: 1313
Fragments arranged by size:
1611 1313 784 529 360 314 245 191
43
NaeI GCC'GGC
Cuts at: 3571 3571
Size: 5390
NarI GG'CG_CC
Cuts at: 2821 3069 2821
Size: 248 5142
NcoI C'CATG_G
Cuts at: 341 654 1159 1562 341
Size: 313 505 403 4169
Fragments arranged by size:
4169 505 403 313
NgoMIV G'CCGG_C
Cuts at: 3569 3569
Size: 5390
NsiI A_TGCA'T
Cuts at: 1287 1287
Size: 5390
NspI r_CATG'y
Cuts at: 1225 1521 1545 1902 2220 2443 2943 4546 1225
Size: 296 24 357 318 223 500 1603 2069
Fragments arranged by size:
2069 1603 500 357 318 296 223 24
PciI A'CATG_T
Cuts at: 1517 4542 1517
Size: 3025 2365
PflMI CCAn_nnn'nTGG
Cuts at: 864 864
Size: 5390
PfoI T'CCnGG_A
Cuts at: 1529 1529
Size: 5390
PmeI GTTT'AAAC
Cuts at: 2389 2389
Size: 5390
PmlI CAC'GTG
Cuts at: 1884 1884
Size: 5390
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 3836
Size: 32 5358
PpuMI rG'GwC_Cy
Cuts at: 456 1022 1407 456
Size: 566 385 4439
Fragments arranged by size:
4439 566 385
PshAI GACnn'nnGTC
Cuts at: 750 750
Size: 5390
PspXI vC'TCGA_Gb
Cuts at: 543 543
Size: 5390
PstI C_TGCA'G
Cuts at: 441 486 734 1792 2437 3122 441
Size: 45 248 1058 645 685 2709
Fragments arranged by size:
2709 1058 685 645 248 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5390
PvuII CAG'CTG
Cuts at: 127 361 890 2815 3175 127
Size: 234 529 1925 360 2342
Fragments arranged by size:
2342 1925 529 360 234
RsrII CG'GwC_CG
Cuts at: 3585 3585
Size: 5390
SacI G_AGCT'C
Cuts at: 2404 2404
Size: 5390
SalI G'TCGA_C
Cuts at: 2427 2427
Size: 5390
SapI GCTCTTCn'nnn_
Cuts at: 3413 3623 3413
Size: 210 5180
SbfI CC_TGCA'GG
Cuts at: 2437 2437
Size: 5390
ScaI AGT'ACT
Cuts at: 119 1891 4907 119
Size: 1772 3016 602
Fragments arranged by size:
3016 1772 602
SfcI C'TryA_G
Cuts at: 33 176 437 482 730 1788 1907 2433 3118
Size: 143 261 45 248 1058 119 526 685
Cuts at: 3118 4086 4277 33
Size: 968 191 1146
Fragments arranged by size:
1146 1058 968 685 526 261 248 191
143 119 45
SfoI GGC'GCC
Cuts at: 2822 3070 2822
Size: 248 5142
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5390
SmaI CCC'GGG
Cuts at: 2412 2412
Size: 5390
SmlI C'TyrA_G
Cuts at: 543 2545 3460 4174 4436 543
Size: 2002 915 714 262 1497
Fragments arranged by size:
2002 1497 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5390
SphI G_CATG'C
Cuts at: 1225 2443 2943 1225
Size: 1218 500 3672
Fragments arranged by size:
3672 1218 500
StuI AGG'CCT
Cuts at: 697 697
Size: 5390
StyI C'CwwG_G
Cuts at: 146 341 642 654 1159 1562 2524 2878 146
Size: 195 301 12 505 403 962 354 2658
Fragments arranged by size:
2658 962 505 403 354 301 195 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 545 936 2080 2973 3106 3565 3774 545
Size: 391 1144 893 133 459 209 2161
Fragments arranged by size:
2161 1144 893 459 391 209 133
TatI w'GTAC_w
Cuts at: 117 1889 4905 117
Size: 1772 3016 602
Fragments arranged by size:
3016 1772 602
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4907 4939 4907
Size: 32 5358
Tth111I GACn'n_nGTC
Cuts at: 1489 3187 1489
Size: 1698 3692
XbaI T'CTAG_A
Cuts at: 2421 2421
Size: 5390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 399 2885 399
Size: 2486 2904
XhoI C'TCGA_G
Cuts at: 543 543
Size: 5390
XmaI C'CCGG_G
Cuts at: 2410 2410
Size: 5390
XmnI GAAnn'nnTTC
Cuts at: 119 119
Size: 5390
ZraI GAC'GTC
Cuts at: 183 2106 183
Size: 1923 3467
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AleI AloI ApaI AscI AvrII
BaeI BglI BmgBI BmtI BsiWI BspEI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI
HindIII MfeI NdeI NheI NotI NruI PacI PasI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI