Restriction map for
pF1KB5636
(Circular) (Six-base) MAPSORT of: pF1KB5636.seq Check: 5744 from: 1 to: 4106
pF1KB5636 4106 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1144 1144
Size: 4106
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1931 2363 3110 3378 1931
Size: 432 747 268 2659
Fragments arranged by size:
2659 747 432 268
AflIII A'CryG_T
Cuts at: 1458 2562 3539 1458
Size: 1104 977 2025
Fragments arranged by size:
2025 1104 977
AgeI A'CCGG_T
Cuts at: 2494 2494
Size: 4106
AlwNI CAG_nnn'CTG
Cuts at: 537 1411 2978 537
Size: 874 1567 1665
Fragments arranged by size:
1665 1567 874
ApaI G_GGCC'C
Cuts at: 404 404
Size: 4106
ApaLI G'TGCA_C
Cuts at: 2876 2876
Size: 4106
ApoI r'AATT_y
Cuts at: 1110 3459 3715 1110
Size: 2349 256 1501
Fragments arranged by size:
2349 1501 256
AscI GG'CGCG_CC
Cuts at: 929 929
Size: 4106
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 285 397 1097 1126 285
Size: 112 700 29 3265
Fragments arranged by size:
3265 700 112 29
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 313 369 856 1122 1536 1784 1819 313
Size: 56 487 266 414 248 35 2600
Fragments arranged by size:
2600 487 414 266 248 56 35
BanII G_rGCy'C
Cuts at: 254 404 737 1031 1120 2150 254
Size: 150 333 294 89 1030 2210
Fragments arranged by size:
2210 1030 333 294 150 89
BbeI G_GCGC'C
Cuts at: 317 1540 1788 317
Size: 1223 248 2635
Fragments arranged by size:
2635 1223 248
BbvCI CC'TCA_GC
Cuts at: 526 526
Size: 4106
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BglI GCCn_nnn'nGGC
Cuts at: 184 290 184
Size: 106 4000
BglII A'GATC_T
Cuts at: 1621 3658 1621
Size: 2037 2069
BlpI GC'TnA_GC
Cuts at: 775 1218 775
Size: 443 3663
Bme1580I G_kGCm'C
Cuts at: 374 404 859 1731 1824 2880 374
Size: 30 455 872 93 1056 1600
Fragments arranged by size:
1600 1056 872 455 93 30
BmgBI CAC'GTC
Cuts at: 957 957
Size: 4106
BmrI ACTGGGnnnn_n'
Cuts at: 250 277 1318 1399 1734 3878 250
Size: 27 1041 81 335 2144 478
Fragments arranged by size:
2144 1041 478 335 81 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 957 957
Size: 4106
Bpu10I CC'TnA_GC
Cuts at: 526 526
Size: 4106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 2161 2653 2951 1282
Size: 879 492 298 2437
Fragments arranged by size:
2437 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 609 3810 609
Size: 3201 905
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 1620 3657 6
Size: 1614 2037 455
Fragments arranged by size:
2037 1614 455
BsaHI Gr'CG_yC
Cuts at: 215 314 1537 1785 2487 3983 215
Size: 99 1223 248 702 1496 338
Fragments arranged by size:
1496 1223 702 338 248 99
BsaWI w'CCGG_w
Cuts at: 1295 1816 2494 2768 2915 3326 1295
Size: 521 678 274 147 411 2075
Fragments arranged by size:
2075 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 627 862 865 1039 627
Size: 235 3 174 3694
Fragments arranged by size:
3694 235 174 3
BseYI C'CCAG_C
Cuts at: 149 860 1531 2289 2866 3362 149
Size: 711 671 758 577 496 893
Fragments arranged by size:
893 758 711 671 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 484 408
Size: 76 4030
BsiEI CG_ry'CG
Cuts at: 104 121 173 242 353 456 507 1648 1694
Size: 17 52 69 111 103 51 1141 46
Cuts at: 1694 2902 104
Size: 1208 1308
Fragments arranged by size:
1308 1208 1141 111 103 69 52 51
46 17
BsiHKAI G_wGCw'C
Cuts at: 737 1120 1898 2088 2880 737
Size: 383 778 190 792 1963
Fragments arranged by size:
1963 792 778 383 190
BsmBI CGTCTCn'nnnn_
Cuts at: 220 220
Size: 4106
Bsp1286I G_dGCh'C
Cuts at: 254 374 404 737 859 1031 1120 1731 1824
Size: 120 30 333 122 172 89 611 93
Cuts at: 1824 1898 2088 2150 2880 254
Size: 74 190 62 730 1480
Fragments arranged by size:
1480 730 611 333 190 172 122 120
93 89 74 62 30
BspEI T'CCGG_A
Cuts at: 3326 3326
Size: 4106
BspHI T'CATG_A
Cuts at: 3282 3282
Size: 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1156 1370 1672 2053 1156
Size: 214 302 381 3209
Fragments arranged by size:
3209 381 302 214
BsrBI CCG'CTC
Cuts at: 50 117 2398 2452 3936 4101 50
Size: 67 2281 54 1484 165 55
Fragments arranged by size:
2281 1484 165 67 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4106
BsrFI r'CCGG_y
Cuts at: 301 1090 2104 2285 2494 301
Size: 789 1014 181 209 1913
Fragments arranged by size:
1913 1014 789 209 181
BssHII G'CGCG_C
Cuts at: 272 274 358 509 929 272
Size: 2 84 151 420 3449
Fragments arranged by size:
3449 420 151 84 2
BssSI C'ACGA_G
Cuts at: 2377 2735 2377
Size: 358 3748
BstAPI GCAn_nnn'nTGC
Cuts at: 957 1483 957
Size: 526 3580
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstYI r'GATC_y
Cuts at: 1 788 1131 1598 1621 1956 2202 3214 3300
Size: 787 343 467 23 335 246 1012 86
Cuts at: 3300 3312 3658 1
Size: 12 346 449
Fragments arranged by size:
1012 787 467 449 346 343 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 711 2056 2237 2400 711
Size: 1345 181 163 2417
Fragments arranged by size:
2417 1345 181 163
BtsI GCAGTG_nn'
Cuts at: 147 1349 147
Size: 1202 2904
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 118 170 239 350 453 504 800 824 1691
Size: 52 69 111 103 51 296 24 867
Cuts at: 1691 1865 2256 2283 3628 118
Size: 174 391 27 1345 596
Fragments arranged by size:
1345 867 596 391 296 174 111 103
69 52 51 27 24
EagI C'GGCC_G
Cuts at: 118 170 239 350 453 504 1691 118
Size: 52 69 111 103 51 1187 2533
Fragments arranged by size:
2533 1187 111 103 69 52 51
EarI CTCTTCn'nnn_
Cuts at: 604 2129 2339 604
Size: 1525 210 2371
Fragments arranged by size:
2371 1525 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 784 2314 2624 2770 3916 784
Size: 1530 310 146 1146 974
Fragments arranged by size:
1530 1146 974 310 146
EcoICRI GAG'CTC
Cuts at: 735 1118 735
Size: 383 3723
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 957 1931 2363 3110 3378 957
Size: 974 432 747 268 1685
Fragments arranged by size:
1685 974 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 722 882 1245 722
Size: 160 363 3583
Fragments arranged by size:
3583 363 160
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 976 2125 976
Size: 1149 2957
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 317 1540 1788 2810 3789 317
Size: 1223 248 1022 979 634
Fragments arranged by size:
1223 1022 979 634 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1948 1978 1980 2010 1948
Size: 30 2 30 4044
Fragments arranged by size:
4044 30 30 2
HincII GTy'rAC
Cuts at: 1145 1145
Size: 4106
KasI G'GCGC_C
Cuts at: 313 1536 1784 313
Size: 1223 248 2635
Fragments arranged by size:
2635 1223 248
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2777 2961 2777
Size: 184 3922
MscI TGG'CCA
Cuts at: 802 826 1867 802
Size: 24 1041 3041
Fragments arranged by size:
3041 1041 24
MslI CAynn'nnrTG
Cuts at: 2095 2222 2095
Size: 127 3979
MspA1I CmG'CkG
Cuts at: 247 436 468 537 554 864 977 1217 1531
Size: 189 32 69 17 310 113 240 314
Cuts at: 1531 1891 2904 3149 247
Size: 360 1013 245 1204
Fragments arranged by size:
1204 1013 360 314 310 245 240 189
113 69 32 17
NaeI GCC'GGC
Cuts at: 1092 2287 1092
Size: 1195 2911
NarI GG'CG_CC
Cuts at: 314 1537 1785 314
Size: 1223 248 2635
Fragments arranged by size:
2635 1223 248
NgoMIV G'CCGG_C
Cuts at: 1090 2285 1090
Size: 1195 2911
NotI GC'GGCC_GC
Cuts at: 118 239 118
Size: 121 3985
NspI r_CATG'y
Cuts at: 1159 1659 2566 1159
Size: 500 907 2699
Fragments arranged by size:
2699 907 500
PciI A'CATG_T
Cuts at: 2562 2562
Size: 4106
PflMI CCAn_nnn'nTGG
Cuts at: 537 537
Size: 4106
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 4074
PpuMI rG'GwC_Cy
Cuts at: 882 882
Size: 4106
PspOMI G'GGCC_C
Cuts at: 400 400
Size: 4106
PstI C_TGCA'G
Cuts at: 1153 1838 1153
Size: 685 3421
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 468 977 1531 1891 468
Size: 509 554 360 2683
Fragments arranged by size:
2683 554 509 360
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 737 1120 737
Size: 383 3723
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SexAI A'CCwGG_T
Cuts at: 197 197
Size: 4106
SfcI C'TryA_G
Cuts at: 33 519 1149 1834 2827 3018 33
Size: 486 630 685 993 191 1121
Fragments arranged by size:
1121 993 685 630 486 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 184 184
Size: 4106
SfoI GGC'GCC
Cuts at: 315 1538 1786 315
Size: 1223 248 2635
Fragments arranged by size:
2635 1223 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 287 399 1128 287
Size: 112 729 3265
Fragments arranged by size:
3265 729 112
SmlI C'TyrA_G
Cuts at: 1261 2176 2668 2930 1261
Size: 915 492 262 2437
Fragments arranged by size:
2437 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3606
StyI C'CwwG_G
Cuts at: 221 1240 1594 221
Size: 1019 354 2733
Fragments arranged by size:
2733 1019 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 874 1689 1822 2281 2490 127
Size: 747 815 133 459 209 1743
Fragments arranged by size:
1743 815 747 459 209 133
TatI w'GTAC_w
Cuts at: 3621 3621
Size: 4106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 4074
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1601 1601
Size: 4106
XmaI C'CCGG_G
Cuts at: 285 397 1126 285
Size: 112 729 3265
Fragments arranged by size:
3265 729 112
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NotI NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AvrII BaeI BbsI BcgI BfrBI BmtI BsaXI BsiWI
BsmI BsrGI BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI