Restriction map for
pF1KB5626
(Circular) (Six-base) MAPSORT of: pF1KB5626.seq Check: 3988 from: 1 to: 4499
pF1KB5626 4499 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 1281 1281
Size: 4499
AccI GT'mk_AC
Cuts at: 1537 1537
Size: 4499
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 557 2324 2756 3109 3771 539
Size: 18 1767 432 353 662 1267
Fragments arranged by size:
1767 1267 662 432 353 18
AflIII A'CryG_T
Cuts at: 1851 3651 3932 1851
Size: 1800 281 2418
Fragments arranged by size:
2418 1800 281
AleI CACnn'nnGTG
Cuts at: 493 493
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 1804 3242 1804
Size: 1438 3061
ApaI G_GGCC'C
Cuts at: 1270 1270
Size: 4499
ApaLI G'TGCA_C
Cuts at: 3337 3337
Size: 4499
ApoI r'AATT_y
Cuts at: 1503 3852 4108 1503
Size: 2349 256 1894
Fragments arranged by size:
2349 1894 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 723 1015 1519 723
Size: 292 504 3703
Fragments arranged by size:
3703 504 292
BamHI G'GATC_C
Cuts at: 1524 1524
Size: 4499
BanI G'GyrC_C
Cuts at: 1515 1929 2177 2212 1515
Size: 414 248 35 3802
Fragments arranged by size:
3802 414 248 35
BanII G_rGCy'C
Cuts at: 205 1270 1513 2543 205
Size: 1065 243 1030 2161
Fragments arranged by size:
2161 1065 1030 243
BbeI G_GCGC'C
Cuts at: 1933 2181 1933
Size: 248 4251
BbsI GAAGACnn'nnnn_
Cuts at: 545 931 545
Size: 386 4113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 840 874 934 968 840
Size: 34 60 34 4371
Fragments arranged by size:
4371 60 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 591 2397 3453 4499 591
Size: 1806 1056 1046 591
Fragments arranged by size:
1806 1056 1046 591
BclI T'GATC_A
Cuts at: 449 2019 449
Size: 1570 2929
BfrBI ATG'CAT
Cuts at: 1427 1427
Size: 4499
BglII A'GATC_T
Cuts at: 1459 2014 4051 1459
Size: 555 2037 1907
Fragments arranged by size:
2037 1907 555
BlpI GC'TnA_GC
Cuts at: 1391 1611 1391
Size: 220 4279
Bme1580I G_kGCm'C
Cuts at: 785 1270 2124 2217 3341 785
Size: 485 854 93 1124 1943
Fragments arranged by size:
1943 1124 854 485 93
BmrI ACTGGGnnnn_n'
Cuts at: 638 1271 1711 1792 2127 4271 638
Size: 633 440 81 335 2144 866
Fragments arranged by size:
2144 866 633 440 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 180 2866 180
Size: 2686 1813
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 779 1047 1191 449
Size: 330 268 144 3757
Fragments arranged by size:
3757 330 268 144
Bpu10I CC'TnA_GC
Cuts at: 1396 1396
Size: 4499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1675 2554 3268 3566 1675
Size: 879 714 298 2608
Fragments arranged by size:
2608 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4203 4203
Size: 4499
BsaAI yAC'GTr
Cuts at: 1044 1852 1044
Size: 808 3691
BsaBI GATnn'nnATC
Cuts at: 6 2013 4050 6
Size: 2007 2037 455
Fragments arranged by size:
2037 2007 455
BsaHI Gr'CG_yC
Cuts at: 1278 1930 2178 2880 4376 1278
Size: 652 248 702 1496 1401
Fragments arranged by size:
1496 1401 702 652 248
BsaWI w'CCGG_w
Cuts at: 1688 2209 2887 3298 3445 3719 1688
Size: 521 678 411 147 274 2468
Fragments arranged by size:
2468 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 533 563 805 835 1045 1075 1140 1170 533
Size: 30 242 30 210 30 65 30 3862
Fragments arranged by size:
3862 242 210 65 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 288 829 1352 288
Size: 541 523 3435
Fragments arranged by size:
3435 541 523
BseYI C'CCAG_C
Cuts at: 204 1924 2682 3347 3755 204
Size: 1720 758 665 408 948
Fragments arranged by size:
1720 948 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2041 2087 3317 104
Size: 1937 46 1230 1286
Fragments arranged by size:
1937 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1513 2291 2481 3341 1513
Size: 778 190 860 2671
Fragments arranged by size:
2671 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 205 785 1270 1513 2124 2217 2291 2481 2543
Size: 580 485 243 611 93 74 190 62
Cuts at: 2543 3341 205
Size: 798 1363
Fragments arranged by size:
1363 798 611 580 485 243 190 93
74 62
BspHI T'CATG_A
Cuts at: 2931 2931
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 244 1549 1763 2065 2446 244
Size: 1305 214 302 381 2297
Fragments arranged by size:
2297 1305 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2791 2845 4329 4494 50
Size: 2741 54 1484 165 55
Fragments arranged by size:
2741 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 980 2411 980
Size: 1431 3068
BsrFI r'CCGG_y
Cuts at: 847 2497 2678 847
Size: 1650 181 2668
Fragments arranged by size:
2668 1650 181
BssSI C'ACGA_G
Cuts at: 1301 2770 3478 1301
Size: 1469 708 2322
Fragments arranged by size:
2322 1469 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1876 1876
Size: 4499
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstYI r'GATC_y
Cuts at: 1 1417 1433 1459 1524 1991 2014 2349 2595
Size: 1416 16 26 65 467 23 335 246
Cuts at: 2595 2901 2913 2999 4051 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1416 1052 467 449 335 306 246 86
65 26 23 16 12
Bsu36I CC'TnA_GG
Cuts at: 1321 1321
Size: 4499
BtgI C'CryG_G
Cuts at: 185 185
Size: 4499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 843 2449 2630 2793 843
Size: 1606 181 163 2549
Fragments arranged by size:
2549 1606 181 163
BtsI GCAGTG_nn'
Cuts at: 1742 1742
Size: 4499
DraI TTT'AAA
Cuts at: 1498 1498
Size: 4499
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3549 2205
Size: 1344 3155
EaeI y'GGCC_r
Cuts at: 2084 2258 2649 2676 4021 2084
Size: 174 391 27 1345 2562
Fragments arranged by size:
2562 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 823 2522 2732 823
Size: 1699 210 2590
Fragments arranged by size:
2590 1699 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 826 2707 3449 3595 4309 826
Size: 1881 742 146 714 1016
Fragments arranged by size:
1881 1016 742 714 146
EcoICRI GAG'CTC
Cuts at: 1511 1511
Size: 4499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 539 557 779 1047 1191 2324 2756 3109
Size: 90 18 222 268 144 1133 432 353
Cuts at: 3109 3771 449
Size: 662 1177
Fragments arranged by size:
1177 1133 662 432 353 268 222 144
90 18
EcoO109I rG'GnC_Cy
Cuts at: 1266 1297 1638 1266
Size: 31 341 4127
Fragments arranged by size:
4127 341 31
EcoRI G'AATT_C
Cuts at: 1503 1503
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4499
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 1933 2181 3411 4182 1933
Size: 248 1230 771 2250
Fragments arranged by size:
2250 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 460 492 624 656 2341 2371 2373 2403 460
Size: 32 132 32 1685 30 2 30 2556
Fragments arranged by size:
2556 1685 132 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1538 1538
Size: 4499
KasI G'GCGC_C
Cuts at: 1929 2177 1929
Size: 248 4251
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3258 3442 3258
Size: 184 4315
MscI TGG'CCA
Cuts at: 2260 2260
Size: 4499
MslI CAynn'nnrTG
Cuts at: 493 520 886 2488 2615 493
Size: 27 366 1602 127 2377
Fragments arranged by size:
2377 1602 366 127 27
MspA1I CmG'CkG
Cuts at: 187 918 1029 1169 1610 1924 2284 3068 3313
Size: 731 111 140 441 314 360 784 245
Cuts at: 3313 187
Size: 1373
Fragments arranged by size:
1373 784 731 441 360 314 245 140
111
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1930 2178 1930
Size: 248 4251
NdeI CA'TA_TG
Cuts at: 546 546
Size: 4499
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NsiI A_TGCA'T
Cuts at: 1429 1429
Size: 4499
NspI r_CATG'y
Cuts at: 1330 1431 1552 2052 3655 1330
Size: 101 121 500 1603 2174
Fragments arranged by size:
2174 1603 500 121 101
PciI A'CATG_T
Cuts at: 3651 3651
Size: 4499
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 4467
PspOMI G'GGCC_C
Cuts at: 1266 1266
Size: 4499
PstI C_TGCA'G
Cuts at: 255 1546 2231 255
Size: 1291 685 2523
Fragments arranged by size:
2523 1291 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 1029 1169 1924 2284 1029
Size: 140 755 360 3244
Fragments arranged by size:
3244 755 360 140
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 1513 1513
Size: 4499
SacII CC_GC'GG
Cuts at: 188 188
Size: 4499
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SfcI C'TryA_G
Cuts at: 33 251 1542 2227 3195 3386 33
Size: 218 1291 685 968 191 1146
Fragments arranged by size:
1291 1146 968 685 218 191
SfoI GGC'GCC
Cuts at: 1931 2179 1931
Size: 248 4251
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 723 1654 2569 3283 3545 723
Size: 931 915 714 262 1677
Fragments arranged by size:
1677 931 915 714 262
SnaBI TAC'GTA
Cuts at: 1044 1044
Size: 4499
SpeI A'CTAG_T
Cuts at: 67 264 67
Size: 197 4302
SphI G_CATG'C
Cuts at: 1330 1431 1552 2052 1330
Size: 101 121 500 3777
Fragments arranged by size:
3777 500 121 101
StyI C'CwwG_G
Cuts at: 1633 1987 1633
Size: 354 4145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 944 1165 2082 2215 2674 2883 944
Size: 221 917 133 459 209 2560
Fragments arranged by size:
2560 917 459 221 209 133
TatI w'GTAC_w
Cuts at: 769 4014 769
Size: 3245 1254
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 533 565 1105 1137 1140 1172 4016 4048 533
Size: 32 540 32 3 32 2844 32 984
Fragments arranged by size:
2844 984 540 32 32 32 32 3
Tth111I GACn'n_nGTC
Cuts at: 2296 2296
Size: 4499
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1476 1994 1476
Size: 518 3981
XhoI C'TCGA_G
Cuts at: 723 723
Size: 4499
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
XmnI GAAnn'nnTTC
Cuts at: 670 670
Size: 4499
ZraI GAC'GTC
Cuts at: 1279 1279
Size: 4499
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI