Restriction map for
pF1KB5623
(Circular) (Six-base) MAPSORT of: pF1KB5623.seq Check: 1250 from: 1 to: 6050
pF1KB5623 6050 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 3088 3088
Size: 6050
Acc65I G'GTAC_C
Cuts at: 2084 3066 2084
Size: 982 5068
AclI AA'CG_TT
Cuts at: 192 5890 192
Size: 5698 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1887 1967 3875 4307 4660 5322 1887
Size: 80 1908 432 353 662 2615
Fragments arranged by size:
2615 1908 662 432 353 80
AflII C'TTAA_G
Cuts at: 1053 1278 1053
Size: 225 5825
AflIII A'CryG_T
Cuts at: 238 687 3402 5202 5483 238
Size: 449 2715 1800 281 805
Fragments arranged by size:
2715 1800 805 449 281
AleI CACnn'nnGTG
Cuts at: 370 370
Size: 6050
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 538 570 538
Size: 32 6018
AlwNI CAG_nnn'CTG
Cuts at: 390 2510 2704 3355 4793 390
Size: 2120 194 651 1438 1647
Fragments arranged by size:
2120 1647 1438 651 194
ApaLI G'TGCA_C
Cuts at: 1171 4888 1171
Size: 3717 2333
ApoI r'AATT_y
Cuts at: 486 1907 2467 3054 5403 5659 486
Size: 1421 560 587 2349 256 877
Fragments arranged by size:
2349 1421 877 587 560 256
AseI AT'TA_AT
Cuts at: 20 2461 20
Size: 2441 3609
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
AvaI C'yCGr_G
Cuts at: 3070 3070
Size: 6050
AvrII C'CTAG_G
Cuts at: 2568 2568
Size: 6050
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 194 227 2068 2101 194
Size: 33 1841 33 4143
Fragments arranged by size:
4143 1841 33 33
BamHI G'GATC_C
Cuts at: 879 3075 879
Size: 2196 3854
BanI G'GyrC_C
Cuts at: 589 2084 2922 3066 3480 3728 3763 589
Size: 1495 838 144 414 248 35 2876
Fragments arranged by size:
2876 1495 838 414 248 144 35
BanII G_rGCy'C
Cuts at: 416 827 1556 3064 4094 416
Size: 411 729 1508 1030 2372
Fragments arranged by size:
2372 1508 1030 729 411
BbeI G_GCGC'C
Cuts at: 3484 3732 3484
Size: 248 5802
BciVI GTATCCnnnnn_n'
Cuts at: 648 1954 2001 2524 2745 3948 5004 6050 648
Size: 1306 47 523 221 1203 1056 1046 648
Fragments arranged by size:
1306 1203 1056 1046 648 523 221 47
BclI T'GATC_A
Cuts at: 3570 3570
Size: 6050
BglII A'GATC_T
Cuts at: 3565 5602 3565
Size: 2037 4013
BlpI GC'TnA_GC
Cuts at: 3162 3162
Size: 6050
Bme1580I G_kGCm'C
Cuts at: 1175 3675 3768 4892 1175
Size: 2500 93 1124 2333
Fragments arranged by size:
2500 2333 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2273 2859 2908 3262 3343 3678 5822 2273
Size: 586 49 354 81 335 2144 2501
Fragments arranged by size:
2501 2144 586 354 335 81 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4417 4417
Size: 6050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 522 1437 1781 2858 513
Size: 9 915 344 1077 3705
Fragments arranged by size:
3705 1077 915 344 9
Bpu10I CC'TnA_GC
Cuts at: 479 479
Size: 6050
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 838 989 3226 4105 4819 5117 180
Size: 658 151 2237 879 714 298 1113
Fragments arranged by size:
2237 1113 879 714 658 298 151
BsaI GGTCTCn'nnnn_
Cuts at: 2404 5754 2404
Size: 3350 2700
BsaAI yAC'GTr
Cuts at: 1998 3403 1998
Size: 1405 4645
BsaBI GATnn'nnATC
Cuts at: 6 316 2335 3564 5601 6
Size: 310 2019 1229 2037 455
Fragments arranged by size:
2037 2019 1229 455 310
BsaHI Gr'CG_yC
Cuts at: 3481 3729 4431 5927 3481
Size: 248 702 1496 3604
Fragments arranged by size:
3604 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2307 3239 3760 4438 4849 4996 5270 2307
Size: 932 521 678 411 147 274 3087
Fragments arranged by size:
3087 932 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2800 2830 2800
Size: 30 6020
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 835 2310 2997 835
Size: 1475 687 3888
Fragments arranged by size:
3888 1475 687
BseYI C'CCAG_C
Cuts at: 401 2757 2832 3475 4233 4898 5306 401
Size: 2356 75 643 758 665 408 1145
Fragments arranged by size:
2356 1145 758 665 643 408 75
BsiEI CG_ry'CG
Cuts at: 104 2990 3592 3638 4868 104
Size: 2886 602 46 1230 1286
Fragments arranged by size:
2886 1286 1230 602 46
BsiHKAI G_wGCw'C
Cuts at: 416 1175 1556 1814 3064 3842 4032 4892 416
Size: 759 381 258 1250 778 190 860 1574
Fragments arranged by size:
1574 1250 860 778 759 381 258 190
BsmI GAATG_Cn'
Cuts at: 697 697
Size: 6050
Bsp1286I G_dGCh'C
Cuts at: 416 827 1175 1556 1814 3064 3675 3768 3842
Size: 411 348 381 258 1250 611 93 74
Cuts at: 3842 4032 4094 4892 416
Size: 190 62 798 1574
Fragments arranged by size:
1574 1250 798 611 411 381 348 258
190 93 74 62
BspEI T'CCGG_A
Cuts at: 2307 2307
Size: 6050
BspHI T'CATG_A
Cuts at: 1157 4482 1157
Size: 3325 2725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1302 3100 3314 3616 3997 1302
Size: 1798 214 302 381 3355
Fragments arranged by size:
3355 1798 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4342 4396 5880 6045 50
Size: 4292 54 1484 165 55
Fragments arranged by size:
4292 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 148 3962 148
Size: 3814 2236
BsrFI r'CCGG_y
Cuts at: 4048 4229 4048
Size: 181 5869
BsrGI T'GTAC_A
Cuts at: 525 970 525
Size: 445 5605
BssSI C'ACGA_G
Cuts at: 4321 5029 4321
Size: 708 5342
BstAPI GCAn_nnn'nTGC
Cuts at: 3427 3427
Size: 6050
BstBI TT'CG_AA
Cuts at: 4411 4411
Size: 6050
BstXI CCAn_nnnn'nTGG
Cuts at: 491 491
Size: 6050
BstYI r'GATC_y
Cuts at: 1 311 879 1499 3024 3075 3542 3565 3900
Size: 310 568 620 1525 51 467 23 335
Cuts at: 3900 4146 4452 4464 4550 5602 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1525 1052 620 568 467 449 335 310
306 246 86 51 23 12
BtgI C'CryG_G
Cuts at: 107 1218 2348 107
Size: 1111 1130 3809
Fragments arranged by size:
3809 1130 1111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4000 4181 4344 4000
Size: 181 163 5706
Fragments arranged by size:
5706 181 163
BtsI GCAGTG_nn'
Cuts at: 893 2122 3293 893
Size: 1229 1171 3650
Fragments arranged by size:
3650 1229 1171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 420 455 420
Size: 35 6015
DraI TTT'AAA
Cuts at: 3049 3049
Size: 6050
DrdI GACnn_nn'nnGTC
Cuts at: 3756 5100 3756
Size: 1344 4706
EaeI y'GGCC_r
Cuts at: 2721 3635 3809 4200 4227 5572 2721
Size: 914 174 391 27 1345 3199
Fragments arranged by size:
3199 1345 914 391 174 27
EagI C'GGCC_G
Cuts at: 3635 3635
Size: 6050
EarI CTCTTCn'nnn_
Cuts at: 1054 4073 4283 1054
Size: 3019 210 2821
Fragments arranged by size:
3019 2821 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4258 5000 5146 5860 4258
Size: 742 146 714 4448
Fragments arranged by size:
4448 742 714 146
EcoICRI GAG'CTC
Cuts at: 414 1554 3062 414
Size: 1140 1508 3402
Fragments arranged by size:
3402 1508 1140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 522 1437 1781 1887 1967 2858 3875 4307
Size: 9 915 344 106 80 891 1017 432
Cuts at: 4307 4660 5322 513
Size: 353 662 1241
Fragments arranged by size:
1241 1017 915 891 662 432 353 344
106 80 9
EcoO109I rG'GnC_Cy
Cuts at: 1404 1840 2275 3189 1404
Size: 436 435 914 4265
Fragments arranged by size:
4265 914 436 435
EcoRI G'AATT_C
Cuts at: 3054 3054
Size: 6050
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 950 1952 4069 950
Size: 1002 2117 2931
Fragments arranged by size:
2931 2117 1002
FspI TGC'GCA
Cuts at: 3831 3831
Size: 6050
HaeII r_GCGC'y
Cuts at: 3484 3732 4962 5733 3484
Size: 248 1230 771 3801
Fragments arranged by size:
3801 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1525 1557 2302 2334 2403 2435 2698 2730 3892
Size: 32 745 32 69 32 263 32 1162
Cuts at: 3892 3922 3924 3954 1525
Size: 30 2 30 3621
Fragments arranged by size:
3621 1162 745 263 69 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1125 2603 3089 1125
Size: 1478 486 4086
Fragments arranged by size:
4086 1478 486
KasI G'GCGC_C
Cuts at: 3480 3728 3480
Size: 248 5802
KpnI G_GTAC'C
Cuts at: 2088 3070 2088
Size: 982 5068
MluI A'CGCG_T
Cuts at: 238 5483 238
Size: 5245 805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2020 2656 4809 4993 2020
Size: 636 2153 184 3077
Fragments arranged by size:
3077 2153 636 184
MscI TGG'CCA
Cuts at: 2723 3811 2723
Size: 1088 4962
MslI CAynn'nnrTG
Cuts at: 349 370 1200 1258 1490 2377 4039 4166 349
Size: 21 830 58 232 887 1662 127 2233
Fragments arranged by size:
2233 1662 887 830 232 127 58 21
MspA1I CmG'CkG
Cuts at: 2836 3161 3475 3835 4619 4864 2836
Size: 325 314 360 784 245 4022
Fragments arranged by size:
4022 784 360 325 314 245
NaeI GCC'GGC
Cuts at: 4231 4231
Size: 6050
NarI GG'CG_CC
Cuts at: 3481 3729 3481
Size: 248 5802
NcoI C'CATG_G
Cuts at: 107 1218 2348 107
Size: 1111 1130 3809
Fragments arranged by size:
3809 1130 1111
NgoMIV G'CCGG_C
Cuts at: 4229 4229
Size: 6050
NspI r_CATG'y
Cuts at: 691 1489 3103 3603 5206 691
Size: 798 1614 500 1603 1535
Fragments arranged by size:
1614 1603 1535 798 500
PasI CC'CwG_GG
Cuts at: 2820 2820
Size: 6050
PciI A'CATG_T
Cuts at: 687 5202 687
Size: 4515 1535
PmeI GTTT'AAAC
Cuts at: 3049 3049
Size: 6050
PmlI CAC'GTG
Cuts at: 1998 1998
Size: 6050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 4496
Size: 32 6018
PpuMI rG'GwC_Cy
Cuts at: 1840 1840
Size: 6050
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1255 1287 1255
Size: 32 6018
PstI C_TGCA'G
Cuts at: 1680 3097 3782 1680
Size: 1417 685 3948
Fragments arranged by size:
3948 1417 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6050
PvuII CAG'CTG
Cuts at: 2836 3475 3835 2836
Size: 639 360 5051
Fragments arranged by size:
5051 639 360
RsrII CG'GwC_CG
Cuts at: 4245 4245
Size: 6050
SacI G_AGCT'C
Cuts at: 416 1556 3064 416
Size: 1140 1508 3402
Fragments arranged by size:
3402 1508 1140
SalI G'TCGA_C
Cuts at: 3087 3087
Size: 6050
SapI GCTCTTCn'nnn_
Cuts at: 4073 4283 4073
Size: 210 5840
SbfI CC_TGCA'GG
Cuts at: 3097 3097
Size: 6050
ScaI AGT'ACT
Cuts at: 815 1273 5567 815
Size: 458 4294 1298
Fragments arranged by size:
4294 1298 458
SexAI A'CCwGG_T
Cuts at: 383 2067 383
Size: 1684 4366
SfcI C'TryA_G
Cuts at: 33 1676 3093 3778 4746 4937 33
Size: 1643 1417 685 968 191 1146
Fragments arranged by size:
1643 1417 1146 968 685 191
SfoI GGC'GCC
Cuts at: 3482 3730 3482
Size: 248 5802
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
SmaI CCC'GGG
Cuts at: 3072 3072
Size: 6050
SmlI C'TyrA_G
Cuts at: 159 817 1004 1053 1278 3205 4120 4834 5096
Size: 658 187 49 225 1927 915 714 262
Cuts at: 5096 159
Size: 1113
Fragments arranged by size:
1927 1113 915 714 658 262 225 187
49
SpeI A'CTAG_T
Cuts at: 67 2260 67
Size: 2193 3857
SphI G_CATG'C
Cuts at: 1489 3103 3603 1489
Size: 1614 500 3936
Fragments arranged by size:
3936 1614 500
StyI C'CwwG_G
Cuts at: 107 827 899 1218 2105 2348 2568 3184 3538
Size: 720 72 319 887 243 220 616 354
Cuts at: 3538 107
Size: 2619
Fragments arranged by size:
2619 887 720 616 354 319 243 220
72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 339 1633 2138 2892 3004 3633 3766 4225 4434
Size: 1294 505 754 112 629 133 459 209
Cuts at: 4434 339
Size: 1955
Fragments arranged by size:
1955 1294 754 629 505 459 209 133
112
TatI w'GTAC_w
Cuts at: 525 813 871 970 1271 1304 2784 5565 525
Size: 288 58 99 301 33 1480 2781 1010
Fragments arranged by size:
2781 1480 1010 301 288 99 58 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1050 1082 1338 1370 5567 5599 1050
Size: 32 256 32 4197 32 1501
Fragments arranged by size:
4197 1501 256 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3847 3847
Size: 6050
XbaI T'CTAG_A
Cuts at: 3081 3081
Size: 6050
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1305 1638 3545 1305
Size: 333 1907 3810
Fragments arranged by size:
3810 1907 333
XmaI C'CCGG_G
Cuts at: 3070 3070
Size: 6050
XmnI GAAnn'nnTTC
Cuts at: 1189 1189
Size: 6050
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI ApaI AscI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PshAI
PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI