Restriction map for
pF1KB5613
(Circular) (Six-base) MAPSORT of: pF1KB5613.seq Check: 9657 from: 1 to: 4616
pF1KB5613 4616 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1654 1654
Size: 4616
Acc65I G'GTAC_C
Cuts at: 1632 1632
Size: 4616
AclI AA'CG_TT
Cuts at: 4456 4456
Size: 4616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 896 2441 2873 3226 3888 247
Size: 649 1545 432 353 662 975
Fragments arranged by size:
1545 975 662 649 432 353
AflIII A'CryG_T
Cuts at: 1968 3768 4049 1968
Size: 1800 281 2535
Fragments arranged by size:
2535 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 523 1321 1921 3359 523
Size: 798 600 1438 1780
Fragments arranged by size:
1780 1438 798 600
ApaLI G'TGCA_C
Cuts at: 3454 3454
Size: 4616
ApoI r'AATT_y
Cuts at: 1047 1275 1620 3969 4225 1047
Size: 228 345 2349 256 1438
Fragments arranged by size:
2349 1438 345 256 228
AseI AT'TA_AT
Cuts at: 20 983 20
Size: 963 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
AvaI C'yCGr_G
Cuts at: 1145 1636 1145
Size: 491 4125
BamHI G'GATC_C
Cuts at: 674 1641 674
Size: 967 3649
BanI G'GyrC_C
Cuts at: 271 1374 1632 2046 2294 2329 271
Size: 1103 258 414 248 35 2558
Fragments arranged by size:
2558 1103 414 258 248 35
BanII G_rGCy'C
Cuts at: 1630 2660 1630
Size: 1030 3586
BbeI G_GCGC'C
Cuts at: 2050 2298 2050
Size: 248 4368
BbvCI CC'TCA_GC
Cuts at: 989 989
Size: 4616
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1328 1362 1328
Size: 34 4582
BciVI GTATCCnnnnn_n'
Cuts at: 2514 3570 4616 2514
Size: 1056 1046 2514
Fragments arranged by size:
2514 1056 1046
BclI T'GATC_A
Cuts at: 2136 2136
Size: 4616
BfrBI ATG'CAT
Cuts at: 841 1359 841
Size: 518 4098
BglI GCCn_nnn'nGGC
Cuts at: 207 207
Size: 4616
BglII A'GATC_T
Cuts at: 2131 4168 2131
Size: 2037 2579
BlpI GC'TnA_GC
Cuts at: 1728 1728
Size: 4616
Bme1580I G_kGCm'C
Cuts at: 2241 2334 3458 2241
Size: 93 1124 3399
Fragments arranged by size:
3399 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1051 1828 1909 2244 4388 1051
Size: 777 81 335 2144 1279
Fragments arranged by size:
2144 1279 777 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 4616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 449 684 752 1107 1274 1505 301
Size: 148 235 68 355 167 231 3412
Fragments arranged by size:
3412 355 235 231 167 148 68
Bpu10I CC'TnA_GC
Cuts at: 989 989
Size: 4616
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1792 2671 3385 3683 1792
Size: 879 714 298 2725
Fragments arranged by size:
2725 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1250 4320 1250
Size: 3070 1546
BsaAI yAC'GTr
Cuts at: 1969 1969
Size: 4616
BsaBI GATnn'nnATC
Cuts at: 6 1358 2130 4167 6
Size: 1352 772 2037 455
Fragments arranged by size:
2037 1352 772 455
BsaHI Gr'CG_yC
Cuts at: 776 2047 2295 2997 4493 776
Size: 1271 248 702 1496 899
Fragments arranged by size:
1496 1271 899 702 248
BsaWI w'CCGG_w
Cuts at: 1805 2326 3004 3415 3562 3836 1805
Size: 521 678 411 147 274 2585
Fragments arranged by size:
2585 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 490 1006 1190 490
Size: 516 184 3916
Fragments arranged by size:
3916 516 184
BseYI C'CCAG_C
Cuts at: 1321 1389 2041 2799 3464 3872 1321
Size: 68 652 758 665 408 2065
Fragments arranged by size:
2065 758 665 652 408 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 241
Size: 4616
BsiEI CG_ry'CG
Cuts at: 104 2158 2204 3434 104
Size: 2054 46 1230 1286
Fragments arranged by size:
2054 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 317 1630 2408 2598 3458 317
Size: 1313 778 190 860 1475
Fragments arranged by size:
1475 1313 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1148 1148
Size: 4616
Bsp1286I G_dGCh'C
Cuts at: 317 1630 2241 2334 2408 2598 2660 3458 317
Size: 1313 611 93 74 190 62 798 1475
Fragments arranged by size:
1475 1313 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3048 3048
Size: 4616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 161 1666 1880 2182 2563 161
Size: 1505 214 302 381 2214
Fragments arranged by size:
2214 1505 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1035 2908 2962 4446 4611 50
Size: 985 1873 54 1484 165 55
Fragments arranged by size:
1873 1484 985 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2528 2528
Size: 4616
BsrFI r'CCGG_y
Cuts at: 2614 2795 2614
Size: 181 4435
BssSI C'ACGA_G
Cuts at: 2887 3595 2887
Size: 708 3908
BstAPI GCAn_nnn'nTGC
Cuts at: 523 1993 523
Size: 1470 3146
BstBI TT'CG_AA
Cuts at: 2977 2977
Size: 4616
BstEII G'GTnAC_C
Cuts at: 148 1153 148
Size: 1005 3611
BstYI r'GATC_y
Cuts at: 1 674 1641 2108 2131 2466 2712 3018 3030
Size: 673 967 467 23 335 246 306 12
Cuts at: 3030 3116 4168 1
Size: 86 1052 449
Fragments arranged by size:
1052 967 673 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 305 305
Size: 4616
BtgI C'CryG_G
Cuts at: 107 926 107
Size: 819 3797
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2566 2747 2910 2566
Size: 181 163 4272
Fragments arranged by size:
4272 181 163
BtsI GCAGTG_nn'
Cuts at: 1019 1025 1859 1019
Size: 6 834 3776
Fragments arranged by size:
3776 834 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 995 1030 995
Size: 35 4581
DraI TTT'AAA
Cuts at: 771 1615 771
Size: 844 3772
DrdI GACnn_nn'nnGTC
Cuts at: 2322 3666 2322
Size: 1344 3272
EaeI y'GGCC_r
Cuts at: 929 2201 2375 2766 2793 4138 929
Size: 1272 174 391 27 1345 1407
Fragments arranged by size:
1407 1345 1272 391 174 27
EagI C'GGCC_G
Cuts at: 2201 2201
Size: 4616
EarI CTCTTCn'nnn_
Cuts at: 223 783 1252 1420 2639 2849 223
Size: 560 469 168 1219 210 1990
Fragments arranged by size:
1990 1219 560 469 210 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2824 3566 3712 4426 2824
Size: 742 146 714 3014
Fragments arranged by size:
3014 742 714 146
EcoICRI GAG'CTC
Cuts at: 1628 1628
Size: 4616
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 301 449 684 752 896 1107 1274 1505
Size: 54 148 235 68 144 211 167 231
Cuts at: 1505 2441 2873 3226 3888 247
Size: 936 432 353 662 975
Fragments arranged by size:
975 936 662 432 353 235 231 211
167 148 144 68 54
EcoO109I rG'GnC_Cy
Cuts at: 369 1755 369
Size: 1386 3230
EcoRI G'AATT_C
Cuts at: 1620 1620
Size: 4616
EcoRV GAT'ATC
Cuts at: 533 533
Size: 4616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 4616
FspI TGC'GCA
Cuts at: 2397 2397
Size: 4616
HaeII r_GCGC'y
Cuts at: 671 2050 2298 3528 4299 671
Size: 1379 248 1230 771 988
Fragments arranged by size:
1379 1230 988 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 275 307 2458 2488 2490 2520 275
Size: 32 2151 30 2 30 2371
Fragments arranged by size:
2371 2151 32 30 30 2
HincII GTy'rAC
Cuts at: 168 1655 168
Size: 1487 3129
HpaI GTT'AAC
Cuts at: 168 168
Size: 4616
KasI G'GCGC_C
Cuts at: 2046 2294 2046
Size: 248 4368
KpnI G_GTAC'C
Cuts at: 1636 1636
Size: 4616
MluI A'CGCG_T
Cuts at: 4049 4049
Size: 4616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3375 3559 3375
Size: 184 4432
MscI TGG'CCA
Cuts at: 931 2377 931
Size: 1446 3170
MslI CAynn'nnrTG
Cuts at: 2605 2732 2605
Size: 127 4489
MspA1I CmG'CkG
Cuts at: 225 616 662 1210 1727 2041 2401 3185 3430
Size: 391 46 548 517 314 360 784 245
Cuts at: 3430 225
Size: 1411
Fragments arranged by size:
1411 784 548 517 391 360 314 245
46
NaeI GCC'GGC
Cuts at: 2797 2797
Size: 4616
NarI GG'CG_CC
Cuts at: 2047 2295 2047
Size: 248 4368
NcoI C'CATG_G
Cuts at: 107 926 107
Size: 819 3797
NdeI CA'TA_TG
Cuts at: 843 843
Size: 4616
NgoMIV G'CCGG_C
Cuts at: 2795 2795
Size: 4616
NsiI A_TGCA'T
Cuts at: 843 1361 843
Size: 518 4098
NspI r_CATG'y
Cuts at: 342 1412 1669 2169 3772 342
Size: 1070 257 500 1603 1186
Fragments arranged by size:
1603 1186 1070 500 257
PasI CC'CwG_GG
Cuts at: 186 186
Size: 4616
PciI A'CATG_T
Cuts at: 3768 3768
Size: 4616
PmeI GTTT'AAAC
Cuts at: 1615 1615
Size: 4616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 4584
PpuMI rG'GwC_Cy
Cuts at: 369 369
Size: 4616
PshAI GACnn'nnGTC
Cuts at: 1486 1486
Size: 4616
PstI C_TGCA'G
Cuts at: 158 519 1663 2348 158
Size: 361 1144 685 2426
Fragments arranged by size:
2426 1144 685 361
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4616
PvuII CAG'CTG
Cuts at: 225 616 662 1210 2041 2401 225
Size: 391 46 548 831 360 2440
Fragments arranged by size:
2440 831 548 391 360 46
RsrII CG'GwC_CG
Cuts at: 2811 2811
Size: 4616
SacI G_AGCT'C
Cuts at: 1630 1630
Size: 4616
SalI G'TCGA_C
Cuts at: 1653 1653
Size: 4616
SapI GCTCTTCn'nnn_
Cuts at: 1252 2639 2849 1252
Size: 1387 210 3019
Fragments arranged by size:
3019 1387 210
SbfI CC_TGCA'GG
Cuts at: 158 1663 158
Size: 1505 3111
ScaI AGT'ACT
Cuts at: 4133 4133
Size: 4616
SfcI C'TryA_G
Cuts at: 33 154 515 629 1659 2344 3312 3503 33
Size: 121 361 114 1030 685 968 191 1146
Fragments arranged by size:
1146 1030 968 685 361 191 121 114
SfoI GGC'GCC
Cuts at: 2048 2296 2048
Size: 248 4368
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
SmaI CCC'GGG
Cuts at: 1638 1638
Size: 4616
SmlI C'TyrA_G
Cuts at: 1145 1771 2686 3400 3662 1145
Size: 626 915 714 262 2099
Fragments arranged by size:
2099 915 714 626 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4616
SphI G_CATG'C
Cuts at: 1669 2169 1669
Size: 500 4116
StyI C'CwwG_G
Cuts at: 107 926 1750 2104 107
Size: 819 824 354 2619
Fragments arranged by size:
2619 824 819 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 480 2199 2332 2791 3000 480
Size: 1719 133 459 209 2096
Fragments arranged by size:
2096 1719 459 209 133
TatI w'GTAC_w
Cuts at: 1453 4131 1453
Size: 2678 1938
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 348 380 1370 1402 4133 4165 348
Size: 32 990 32 2731 32 799
Fragments arranged by size:
2731 990 799 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2413 2413
Size: 4616
XbaI T'CTAG_A
Cuts at: 1647 1647
Size: 4616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 2111
Size: 4616
XhoI C'TCGA_G
Cuts at: 1145 1145
Size: 4616
XmaI C'CCGG_G
Cuts at: 1636 1636
Size: 4616
XmnI GAAnn'nnTTC
Cuts at: 792 792
Size: 4616
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BmgBI BmtI
BsaXI BsiWI BsmI BspEI BsrGI BssHII BstXI BstZ17I
ClaI DraIII EcoNI FseI FspAI HindIII MfeI NheI
NotI NruI PacI PflMI PfoI PmlI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI