Restriction map for
pF1KB5603
(Circular) (Six-base) MAPSORT of: pF1KB5603.seq Check: 2776 from: 1 to: 4511
pF1KB5603 4511 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1549 1549
Size: 4511
Acc65I G'GTAC_C
Cuts at: 1527 1527
Size: 4511
AclI AA'CG_TT
Cuts at: 4351 4351
Size: 4511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 644 2336 2768 3121 3783 197
Size: 447 1692 432 353 662 925
Fragments arranged by size:
1692 925 662 447 432 353
AflIII A'CryG_T
Cuts at: 462 894 1863 3663 3944 462
Size: 432 969 1800 281 1029
Fragments arranged by size:
1800 1029 969 432 281
AlwNI CAG_nnn'CTG
Cuts at: 1816 3254 1816
Size: 1438 3073
ApaLI G'TGCA_C
Cuts at: 3349 3349
Size: 4511
ApoI r'AATT_y
Cuts at: 268 967 1108 1515 3864 4120 268
Size: 699 141 407 2349 256 659
Fragments arranged by size:
2349 699 659 407 256 141
AseI AT'TA_AT
Cuts at: 20 20
Size: 4511
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
AvaI C'yCGr_G
Cuts at: 401 1531 401
Size: 1130 3381
BamHI G'GATC_C
Cuts at: 1536 1536
Size: 4511
BanI G'GyrC_C
Cuts at: 1220 1527 1941 2189 2224 1220
Size: 307 414 248 35 3507
Fragments arranged by size:
3507 414 307 248 35
BanII G_rGCy'C
Cuts at: 1525 2555 1525
Size: 1030 3481
BbeI G_GCGC'C
Cuts at: 1945 2193 1945
Size: 248 4263
BbsI GAAGACnn'nnnn_
Cuts at: 248 543 863 248
Size: 295 320 3896
Fragments arranged by size:
3896 320 295
BciVI GTATCCnnnnn_n'
Cuts at: 2409 3465 4511 2409
Size: 1056 1046 2409
Fragments arranged by size:
2409 1056 1046
BclI T'GATC_A
Cuts at: 353 2031 353
Size: 1678 2833
BglII A'GATC_T
Cuts at: 1293 2026 4063 1293
Size: 733 2037 1741
Fragments arranged by size:
2037 1741 733
BlpI GC'TnA_GC
Cuts at: 1623 1623
Size: 4511
Bme1580I G_kGCm'C
Cuts at: 2136 2229 3353 2136
Size: 93 1124 3294
Fragments arranged by size:
3294 1124 93
BmgBI CAC'GTC
Cuts at: 359 359
Size: 4511
BmrI ACTGGGnnnn_n'
Cuts at: 870 1723 1804 2139 4283 870
Size: 853 81 335 2144 1098
Fragments arranged by size:
2144 1098 853 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2878 2878
Size: 4511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 794 1444 587
Size: 207 650 3654
Fragments arranged by size:
3654 650 207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1687 2566 3280 3578 1687
Size: 879 714 298 2620
Fragments arranged by size:
2620 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 723 4215 723
Size: 3492 1019
BsaAI yAC'GTr
Cuts at: 1864 1864
Size: 4511
BsaBI GATnn'nnATC
Cuts at: 6 2025 4062 6
Size: 2019 2037 455
Fragments arranged by size:
2037 2019 455
BsaHI Gr'CG_yC
Cuts at: 1942 2190 2892 4388 1942
Size: 248 702 1496 2065
Fragments arranged by size:
2065 1496 702 248
BsaWI w'CCGG_w
Cuts at: 963 1700 2221 2899 3310 3457 3731 963
Size: 737 521 678 411 147 274 1743
Fragments arranged by size:
1743 737 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 233 233
Size: 4511
BseYI C'CCAG_C
Cuts at: 1936 2694 3359 3767 1936
Size: 758 665 408 2680
Fragments arranged by size:
2680 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2053 2099 3329 104
Size: 1949 46 1230 1286
Fragments arranged by size:
1949 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1525 2303 2493 3353 1525
Size: 778 190 860 2683
Fragments arranged by size:
2683 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1525 2136 2229 2303 2493 2555 3353 1525
Size: 611 93 74 190 62 798 2683
Fragments arranged by size:
2683 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2943 2943
Size: 4511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 942 1561 1775 2077 2458 942
Size: 619 214 302 381 2995
Fragments arranged by size:
2995 619 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2803 2857 4341 4506 50
Size: 2753 54 1484 165 55
Fragments arranged by size:
2753 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2423 2423
Size: 4511
BsrFI r'CCGG_y
Cuts at: 244 1223 2509 2690 244
Size: 979 1286 181 2065
Fragments arranged by size:
2065 1286 979 181
BsrGI T'GTAC_A
Cuts at: 1251 1251
Size: 4511
BssSI C'ACGA_G
Cuts at: 2782 3490 2782
Size: 708 3803
BstAPI GCAn_nnn'nTGC
Cuts at: 1491 1888 1491
Size: 397 4114
BstBI TT'CG_AA
Cuts at: 2872 2872
Size: 4511
BstYI r'GATC_y
Cuts at: 1 1293 1536 2003 2026 2361 2607 2913 2925
Size: 1292 243 467 23 335 246 306 12
Cuts at: 2925 3011 4063 1
Size: 86 1052 449
Fragments arranged by size:
1292 1052 467 449 335 306 246 243
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 290 290
Size: 4511
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2461 2642 2805 2461
Size: 181 163 4167
Fragments arranged by size:
4167 181 163
BtsI GCAGTG_nn'
Cuts at: 164 1754 164
Size: 1590 2921
DraI TTT'AAA
Cuts at: 1510 1510
Size: 4511
DrdI GACnn_nn'nnGTC
Cuts at: 416 2217 3561 416
Size: 1801 1344 1366
Fragments arranged by size:
1801 1366 1344
EaeI y'GGCC_r
Cuts at: 2096 2270 2661 2688 4033 2096
Size: 174 391 27 1345 2574
Fragments arranged by size:
2574 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2096 2096
Size: 4511
EarI CTCTTCn'nnn_
Cuts at: 290 944 1284 2534 2744 290
Size: 654 340 1250 210 2057
Fragments arranged by size:
2057 1250 654 340 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2719 3461 3607 4321 2719
Size: 742 146 714 2909
Fragments arranged by size:
2909 742 714 146
EcoICRI GAG'CTC
Cuts at: 1523 1523
Size: 4511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 587 644 794 1444 2336 2768 3121 3783
Size: 390 57 150 650 892 432 353 662
Cuts at: 3783 197
Size: 925
Fragments arranged by size:
925 892 662 650 432 390 353 150
57
EcoNI CCTnn'n_nnAGG
Cuts at: 215 215
Size: 4511
EcoO109I rG'GnC_Cy
Cuts at: 1650 1650
Size: 4511
EcoRI G'AATT_C
Cuts at: 967 1108 1515 967
Size: 141 407 3963
Fragments arranged by size:
3963 407 141
EcoRV GAT'ATC
Cuts at: 398 783 398
Size: 385 4126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 539 2530 539
Size: 1991 2520
FspI TGC'GCA
Cuts at: 2292 2292
Size: 4511
HaeII r_GCGC'y
Cuts at: 1945 2193 3423 4194 1945
Size: 248 1230 771 2262
Fragments arranged by size:
2262 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 291 323 1448 1480 2353 2383 2385 2415 291
Size: 32 1125 32 873 30 2 30 2387
Fragments arranged by size:
2387 1125 873 32 32 30 30 2
HincII GTy'rAC
Cuts at: 362 1550 362
Size: 1188 3323
HindIII A'AGCT_T
Cuts at: 128 1429 128
Size: 1301 3210
KasI G'GCGC_C
Cuts at: 1941 2189 1941
Size: 248 4263
KpnI G_GTAC'C
Cuts at: 1531 1531
Size: 4511
MluI A'CGCG_T
Cuts at: 3944 3944
Size: 4511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3270 3454 3270
Size: 184 4327
MscI TGG'CCA
Cuts at: 2272 2272
Size: 4511
MslI CAynn'nnrTG
Cuts at: 2500 2627 2500
Size: 127 4384
MspA1I CmG'CkG
Cuts at: 1622 1936 2296 3080 3325 1622
Size: 314 360 784 245 2808
Fragments arranged by size:
2808 784 360 314 245
NaeI GCC'GGC
Cuts at: 2692 2692
Size: 4511
NarI GG'CG_CC
Cuts at: 1942 2190 1942
Size: 248 4263
NgoMIV G'CCGG_C
Cuts at: 2690 2690
Size: 4511
NspI r_CATG'y
Cuts at: 466 898 1564 2064 3667 466
Size: 432 666 500 1603 1310
Fragments arranged by size:
1603 1310 666 500 432
PciI A'CATG_T
Cuts at: 462 894 3663 462
Size: 432 2769 1310
Fragments arranged by size:
2769 1310 432
PflMI CCAn_nnn'nTGG
Cuts at: 817 817
Size: 4511
PfoI T'CCnGG_A
Cuts at: 603 1112 1421 603
Size: 509 309 3693
Fragments arranged by size:
3693 509 309
PmeI GTTT'AAAC
Cuts at: 1510 1510
Size: 4511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 4479
PstI C_TGCA'G
Cuts at: 1558 2243 1558
Size: 685 3826
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4511
PvuII CAG'CTG
Cuts at: 1936 2296 1936
Size: 360 4151
RsrII CG'GwC_CG
Cuts at: 2706 2706
Size: 4511
SacI G_AGCT'C
Cuts at: 1525 1525
Size: 4511
SalI G'TCGA_C
Cuts at: 1548 1548
Size: 4511
SapI GCTCTTCn'nnn_
Cuts at: 944 2534 2744 944
Size: 1590 210 2711
Fragments arranged by size:
2711 1590 210
SbfI CC_TGCA'GG
Cuts at: 1558 1558
Size: 4511
ScaI AGT'ACT
Cuts at: 678 4028 678
Size: 3350 1161
SfcI C'TryA_G
Cuts at: 33 1364 1554 2239 3207 3398 33
Size: 1331 190 685 968 191 1146
Fragments arranged by size:
1331 1146 968 685 191 190
SfoI GGC'GCC
Cuts at: 1943 2191 1943
Size: 248 4263
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
SmaI CCC'GGG
Cuts at: 1533 1533
Size: 4511
SmlI C'TyrA_G
Cuts at: 401 1666 2581 3295 3557 401
Size: 1265 915 714 262 1355
Fragments arranged by size:
1355 1265 915 714 262
SpeI A'CTAG_T
Cuts at: 67 999 67
Size: 932 3579
SphI G_CATG'C
Cuts at: 1564 2064 1564
Size: 500 4011
SspI AAT'ATT
Cuts at: 187 1281 187
Size: 1094 3417
StyI C'CwwG_G
Cuts at: 514 1645 1999 514
Size: 1131 354 3026
Fragments arranged by size:
3026 1131 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2094 2227 2686 2895 2094
Size: 133 459 209 3710
Fragments arranged by size:
3710 459 209 133
TatI w'GTAC_w
Cuts at: 676 1251 4026 676
Size: 575 2775 1161
Fragments arranged by size:
2775 1161 575
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 599 631 4028 4060 599
Size: 32 3397 32 1050
Fragments arranged by size:
3397 1050 32 32
Tth111I GACn'n_nGTC
Cuts at: 2308 2308
Size: 4511
XbaI T'CTAG_A
Cuts at: 1542 1542
Size: 4511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2006 2006
Size: 4511
XhoI C'TCGA_G
Cuts at: 401 401
Size: 4511
XmaI C'CCGG_G
Cuts at: 1531 1531
Size: 4511
XmnI GAAnn'nnTTC
Cuts at: 1451 1451
Size: 4511
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BglI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BspEI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI
DraIII FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI ZraI