Restriction map for
pF1KB5596
(Circular) (Six-base) MAPSORT of: pF1KB5596.seq Check: 460 from: 1 to: 5096
pF1KB5596 5096 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1283 1283
Size: 5096
AccI GT'mk_AC
Cuts at: 2134 2134
Size: 5096
Acc65I G'GTAC_C
Cuts at: 2112 2112
Size: 5096
AclI AA'CG_TT
Cuts at: 715 4936 715
Size: 4221 875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 2921 3353 3706 4368 949
Size: 1972 432 353 662 1677
Fragments arranged by size:
1972 1677 662 432 353
AfeI AGC'GCT
Cuts at: 1486 1486
Size: 5096
AflIII A'CryG_T
Cuts at: 1945 2448 4248 4529 1945
Size: 503 1800 281 2512
Fragments arranged by size:
2512 1800 503 281
AhdI GACnn_n'nnGTC
Cuts at: 1499 1499
Size: 5096
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1589 1621 1589
Size: 32 5064
AlwNI CAG_nnn'CTG
Cuts at: 2401 3839 2401
Size: 1438 3658
ApaLI G'TGCA_C
Cuts at: 3934 3934
Size: 5096
ApoI r'AATT_y
Cuts at: 592 1192 1631 2100 4449 4705 592
Size: 600 439 469 2349 256 983
Fragments arranged by size:
2349 983 600 469 439 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5096
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5096
AvaI C'yCGr_G
Cuts at: 2116 2116
Size: 5096
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1226 1259 1226
Size: 33 5063
BamHI G'GATC_C
Cuts at: 2121 2121
Size: 5096
BanI G'GyrC_C
Cuts at: 1052 2112 2526 2774 2809 1052
Size: 1060 414 248 35 3339
Fragments arranged by size:
3339 1060 414 248 35
BanII G_rGCy'C
Cuts at: 284 527 2110 3140 284
Size: 243 1583 1030 2240
Fragments arranged by size:
2240 1583 1030 243
BbeI G_GCGC'C
Cuts at: 2530 2778 2530
Size: 248 4848
BbsI GAAGACnn'nnnn_
Cuts at: 902 1451 1862 902
Size: 549 411 4136
Fragments arranged by size:
4136 549 411
BbvCI CC'TCA_GC
Cuts at: 372 372
Size: 5096
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5062
BciVI GTATCCnnnnn_n'
Cuts at: 1260 2994 4050 5096 1260
Size: 1734 1056 1046 1260
Fragments arranged by size:
1734 1260 1056 1046
BclI T'GATC_A
Cuts at: 2616 2616
Size: 5096
BglI GCCn_nnn'nGGC
Cuts at: 112 121 112
Size: 9 5087
BglII A'GATC_T
Cuts at: 2611 4648 2611
Size: 2037 3059
BlpI GC'TnA_GC
Cuts at: 2208 2208
Size: 5096
Bme1580I G_kGCm'C
Cuts at: 1943 2721 2814 3938 1943
Size: 778 93 1124 3101
Fragments arranged by size:
3101 1124 778 93
BmrI ACTGGGnnnn_n'
Cuts at: 445 465 630 2308 2389 2724 4868 445
Size: 20 165 1678 81 335 2144 673
Fragments arranged by size:
2144 1678 673 335 165 81 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1454 3463 1454
Size: 2009 3087
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 817 1517 799
Size: 18 700 4378
Fragments arranged by size:
4378 700 18
Bpu10I CC'TnA_GC
Cuts at: 372 519 372
Size: 147 4949
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 1608 2272 3151 3865 4163 433
Size: 1175 664 879 714 298 1366
Fragments arranged by size:
1366 1175 879 714 664 298
BsaI GGTCTCn'nnnn_
Cuts at: 4800 4800
Size: 5096
BsaAI yAC'GTr
Cuts at: 904 1948 2449 904
Size: 1044 501 3551
Fragments arranged by size:
3551 1044 501
BsaBI GATnn'nnATC
Cuts at: 6 1082 1538 2610 4647 6
Size: 1076 456 1072 2037 455
Fragments arranged by size:
2037 1076 1072 456 455
BsaHI Gr'CG_yC
Cuts at: 829 2527 2775 3477 4973 829
Size: 1698 248 702 1496 952
Fragments arranged by size:
1698 1496 952 702 248
BsaWI w'CCGG_w
Cuts at: 1263 2285 2806 3484 3895 4042 4316 1263
Size: 1022 521 678 411 147 274 2043
Fragments arranged by size:
2043 1022 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1001 1031 1001
Size: 30 5066
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 143 146 149 152 176 179 402 535 662
Size: 3 3 3 24 3 223 133 127
Cuts at: 662 143
Size: 4577
Fragments arranged by size:
4577 223 133 127 24 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2521 3279 3944 4352 2521
Size: 758 665 408 3265
Fragments arranged by size:
3265 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 362 2638 2684 3914 104
Size: 258 2276 46 1230 1286
Fragments arranged by size:
2276 1286 1230 258 46
BsiHKAI G_wGCw'C
Cuts at: 2110 2888 3078 3938 2110
Size: 778 190 860 3268
Fragments arranged by size:
3268 860 778 190
BsmI GAATG_Cn'
Cuts at: 666 854 2077 666
Size: 188 1223 3685
Fragments arranged by size:
3685 1223 188
Bsp1286I G_dGCh'C
Cuts at: 284 527 1943 2110 2721 2814 2888 3078 3140
Size: 243 1416 167 611 93 74 190 62
Cuts at: 3140 3938 284
Size: 798 1442
Fragments arranged by size:
1442 1416 798 611 243 190 167 93
74 62
BspEI T'CCGG_A
Cuts at: 1263 1263
Size: 5096
BspHI T'CATG_A
Cuts at: 1440 3528 1440
Size: 2088 3008
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1283 2146 2360 2662 3043 1283
Size: 863 214 302 381 3336
Fragments arranged by size:
3336 863 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3388 3442 4926 5091 50
Size: 3338 54 1484 165 55
Fragments arranged by size:
3338 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1579 3008 1579
Size: 1429 3667
BsrFI r'CCGG_y
Cuts at: 3094 3275 3094
Size: 181 4915
BsrGI T'GTAC_A
Cuts at: 1401 1401
Size: 5096
BssSI C'ACGA_G
Cuts at: 3367 4075 3367
Size: 708 4388
BstAPI GCAn_nnn'nTGC
Cuts at: 2473 2473
Size: 5096
BstBI TT'CG_AA
Cuts at: 3457 3457
Size: 5096
BstXI CCAn_nnnn'nTGG
Cuts at: 277 277
Size: 5096
BstYI r'GATC_y
Cuts at: 1 2121 2588 2611 2946 3192 3498 3510 3596
Size: 2120 467 23 335 246 306 12 86
Cuts at: 3596 4648 1
Size: 1052 449
Fragments arranged by size:
2120 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 254 1000 254
Size: 746 4350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1848 3046 3227 3390 1848
Size: 1198 181 163 3554
Fragments arranged by size:
3554 1198 181 163
BtsI GCAGTG_nn'
Cuts at: 1881 2339 1881
Size: 458 4638
DraI TTT'AAA
Cuts at: 2095 2095
Size: 5096
DrdI GACnn_nn'nnGTC
Cuts at: 2802 4146 2802
Size: 1344 3752
EaeI y'GGCC_r
Cuts at: 359 422 1719 1994 2681 2855 3246 3273 4618
Size: 63 1297 275 687 174 391 27 1345
Cuts at: 4618 359
Size: 837
Fragments arranged by size:
1345 1297 837 687 391 275 174 63
27
EagI C'GGCC_G
Cuts at: 359 2681 359
Size: 2322 2774
EarI CTCTTCn'nnn_
Cuts at: 420 2060 3119 3329 420
Size: 1640 1059 210 2187
Fragments arranged by size:
2187 1640 1059 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 282 291 384 3304 4046 4192 4906 282
Size: 9 93 2920 742 146 714 472
Fragments arranged by size:
2920 742 714 472 146 93 9
EcoICRI GAG'CTC
Cuts at: 2108 2108
Size: 5096
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 817 949 1517 2921 3353 3706 4368 799
Size: 18 132 568 1404 432 353 662 1527
Fragments arranged by size:
1527 1404 662 568 432 353 132 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1024 1024
Size: 5096
EcoO109I rG'GnC_Cy
Cuts at: 261 792 1653 2235 261
Size: 531 861 582 3122
Fragments arranged by size:
3122 861 582 531
EcoRI G'AATT_C
Cuts at: 1631 2100 1631
Size: 469 4627
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1481 3115 1481
Size: 1634 3462
FspI TGC'GCA
Cuts at: 2877 2877
Size: 5096
HaeII r_GCGC'y
Cuts at: 1488 2003 2530 2778 4008 4779 1488
Size: 515 527 248 1230 771 1805
Fragments arranged by size:
1805 1230 771 527 515 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 525 557 1081 1113 1363 1395 1485 1517 2938
Size: 32 524 32 250 32 90 32 1421
Cuts at: 2938 2968 2970 3000 525
Size: 30 2 30 2621
Fragments arranged by size:
2621 1421 524 250 90 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2135 2135
Size: 5096
KasI G'GCGC_C
Cuts at: 2526 2774 2526
Size: 248 4848
KpnI G_GTAC'C
Cuts at: 2116 2116
Size: 5096
MfeI C'AATT_G
Cuts at: 1922 1922
Size: 5096
MluI A'CGCG_T
Cuts at: 4529 4529
Size: 5096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3855 4039 3855
Size: 184 4912
MscI TGG'CCA
Cuts at: 424 1996 2857 424
Size: 1572 861 2663
Fragments arranged by size:
2663 1572 861
MslI CAynn'nnrTG
Cuts at: 275 512 1172 1310 3085 3212 275
Size: 237 660 138 1775 127 2159
Fragments arranged by size:
2159 1775 660 237 138 127
MspA1I CmG'CkG
Cuts at: 256 388 834 2207 2521 2881 3665 3910 256
Size: 132 446 1373 314 360 784 245 1442
Fragments arranged by size:
1442 1373 784 446 360 314 245 132
NaeI GCC'GGC
Cuts at: 3277 3277
Size: 5096
NarI GG'CG_CC
Cuts at: 2527 2775 2527
Size: 248 4848
NdeI CA'TA_TG
Cuts at: 1249 1249
Size: 5096
NgoMIV G'CCGG_C
Cuts at: 3275 3275
Size: 5096
NotI GC'GGCC_GC
Cuts at: 359 359
Size: 5096
NspI r_CATG'y
Cuts at: 1309 2149 2649 4252 1309
Size: 840 500 1603 2153
Fragments arranged by size:
2153 1603 840 500
PciI A'CATG_T
Cuts at: 4248 4248
Size: 5096
PfoI T'CCnGG_A
Cuts at: 794 794
Size: 5096
PmeI GTTT'AAAC
Cuts at: 2095 2095
Size: 5096
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 5064
PpuMI rG'GwC_Cy
Cuts at: 261 792 1653 261
Size: 531 861 3704
Fragments arranged by size:
3704 861 531
PstI C_TGCA'G
Cuts at: 758 2143 2828 758
Size: 1385 685 3026
Fragments arranged by size:
3026 1385 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5096
PvuII CAG'CTG
Cuts at: 388 2521 2881 388
Size: 2133 360 2603
Fragments arranged by size:
2603 2133 360
RsrII CG'GwC_CG
Cuts at: 3291 3291
Size: 5096
SacI G_AGCT'C
Cuts at: 2110 2110
Size: 5096
SacII CC_GC'GG
Cuts at: 257 257
Size: 5096
SalI G'TCGA_C
Cuts at: 2133 2133
Size: 5096
SapI GCTCTTCn'nnn_
Cuts at: 3119 3329 3119
Size: 210 4886
SbfI CC_TGCA'GG
Cuts at: 2143 2143
Size: 5096
ScaI AGT'ACT
Cuts at: 4613 4613
Size: 5096
SfcI C'TryA_G
Cuts at: 33 754 2139 2824 3792 3983 33
Size: 721 1385 685 968 191 1146
Fragments arranged by size:
1385 1146 968 721 685 191
SfoI GGC'GCC
Cuts at: 2528 2776 2528
Size: 248 4848
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5096
SmaI CCC'GGG
Cuts at: 2118 2118
Size: 5096
SmlI C'TyrA_G
Cuts at: 448 1587 2251 3166 3880 4142 448
Size: 1139 664 915 714 262 1402
Fragments arranged by size:
1402 1139 915 714 664 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5096
SphI G_CATG'C
Cuts at: 2149 2649 2149
Size: 500 4596
SspI AAT'ATT
Cuts at: 2061 2061
Size: 5096
StyI C'CwwG_G
Cuts at: 419 2230 2584 419
Size: 1811 354 2931
Fragments arranged by size:
2931 1811 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2679 2812 3271 3480 2679
Size: 133 459 209 4295
Fragments arranged by size:
4295 459 209 133
TatI w'GTAC_w
Cuts at: 1401 1674 4611 1401
Size: 273 2937 1886
Fragments arranged by size:
2937 1886 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 716 748 4613 4645 716
Size: 32 3865 32 1167
Fragments arranged by size:
3865 1167 32 32
Tth111I GACn'n_nGTC
Cuts at: 2893 2893
Size: 5096
XbaI T'CTAG_A
Cuts at: 1086 2127 1086
Size: 1041 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 432 2591 432
Size: 2159 2937
XmaI C'CCGG_G
Cuts at: 2116 2116
Size: 5096
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NotI NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AleI AlfI ApaI AscI AvrII
BfrBI BmgBI BmtI BsgI BsiWI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NcoI NheI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI