Restriction map for
pF1KB5576
(Circular) (Six-base) MAPSORT of: pF1KB5576.seq Check: 3627 from: 1 to: 3605
pF1KB5576 3605 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 411 411
Size: 3605
AccI GT'mk_AC
Cuts at: 643 643
Size: 3605
Acc65I G'GTAC_C
Cuts at: 621 621
Size: 3605
AclI AA'CG_TT
Cuts at: 3445 3445
Size: 3605
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 1430 1862 2609 2877 509
Size: 921 432 747 268 1237
Fragments arranged by size:
1237 921 747 432 268
AflIII A'CryG_T
Cuts at: 957 2061 3038 957
Size: 1104 977 1524
Fragments arranged by size:
1524 1104 977
AgeI A'CCGG_T
Cuts at: 1993 1993
Size: 3605
AlwNI CAG_nnn'CTG
Cuts at: 910 2477 910
Size: 1567 2038
ApaI G_GGCC'C
Cuts at: 353 353
Size: 3605
ApaLI G'TGCA_C
Cuts at: 2375 2375
Size: 3605
ApoI r'AATT_y
Cuts at: 609 2958 3214 609
Size: 2349 256 1000
Fragments arranged by size:
2349 1000 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3605
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3605
AvaI C'yCGr_G
Cuts at: 625 625
Size: 3605
BamHI G'GATC_C
Cuts at: 630 630
Size: 3605
BanI G'GyrC_C
Cuts at: 394 621 1035 1283 1318 394
Size: 227 414 248 35 2681
Fragments arranged by size:
2681 414 248 227 35
BanII G_rGCy'C
Cuts at: 353 619 1649 353
Size: 266 1030 2309
Fragments arranged by size:
2309 1030 266
BbeI G_GCGC'C
Cuts at: 1039 1287 1039
Size: 248 3357
BbsI GAAGACnn'nnnn_
Cuts at: 437 437
Size: 3605
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 193 227 193
Size: 34 3571
BciVI GTATCCnnnnn_n'
Cuts at: 1503 2264 3605 1503
Size: 761 1341 1503
Fragments arranged by size:
1503 1341 761
BclI T'GATC_A
Cuts at: 1125 1125
Size: 3605
BglI GCCn_nnn'nGGC
Cuts at: 173 173
Size: 3605
BglII A'GATC_T
Cuts at: 1120 3157 1120
Size: 2037 1568
BlpI GC'TnA_GC
Cuts at: 717 717
Size: 3605
Bme1580I G_kGCm'C
Cuts at: 353 1230 1323 2379 353
Size: 877 93 1056 1579
Fragments arranged by size:
1579 1056 877 93
BmrI ACTGGGnnnn_n'
Cuts at: 172 817 898 1233 3377 172
Size: 645 81 335 2144 400
Fragments arranged by size:
2144 645 400 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 530 304
Size: 226 3379
Bpu10I CC'TnA_GC
Cuts at: 474 474
Size: 3605
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1660 2152 2450 781
Size: 879 492 298 1936
Fragments arranged by size:
1936 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3309 3309
Size: 3605
BsaAI yAC'GTr
Cuts at: 958 958
Size: 3605
BsaBI GATnn'nnATC
Cuts at: 6 1119 3156 6
Size: 1113 2037 455
Fragments arranged by size:
2037 1113 455
BsaHI Gr'CG_yC
Cuts at: 1036 1284 1986 3482 1036
Size: 248 702 1496 1159
Fragments arranged by size:
1496 1159 702 248
BsaWI w'CCGG_w
Cuts at: 794 1315 1993 2267 2414 2825 794
Size: 521 678 274 147 411 1574
Fragments arranged by size:
1574 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 392 392
Size: 3605
BseYI C'CCAG_C
Cuts at: 121 1030 1788 2365 2861 121
Size: 909 758 577 496 865
Fragments arranged by size:
909 865 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1147 1193 2401 104
Size: 1043 46 1208 1308
Fragments arranged by size:
1308 1208 1043 46
BsiHKAI G_wGCw'C
Cuts at: 619 1397 1587 2379 619
Size: 778 190 792 1845
Fragments arranged by size:
1845 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 192 192
Size: 3605
Bsp1286I G_dGCh'C
Cuts at: 353 619 1230 1323 1397 1587 1649 2379 353
Size: 266 611 93 74 190 62 730 1579
Fragments arranged by size:
1579 730 611 266 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2825 2825
Size: 3605
BspHI T'CATG_A
Cuts at: 2781 2781
Size: 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 411 655 869 1171 1552 411
Size: 244 214 302 381 2464
Fragments arranged by size:
2464 381 302 244 214
BsrBI CCG'CTC
Cuts at: 50 1897 1951 3435 3600 50
Size: 1847 54 1484 165 55
Fragments arranged by size:
1847 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1517 1517
Size: 3605
BsrFI r'CCGG_y
Cuts at: 117 572 1603 1784 1993 117
Size: 455 1031 181 209 1729
Fragments arranged by size:
1729 1031 455 209 181
BssSI C'ACGA_G
Cuts at: 1876 2234 1876
Size: 358 3247
BstAPI GCAn_nnn'nTGC
Cuts at: 158 982 158
Size: 824 2781
BstBI TT'CG_AA
Cuts at: 1966 1966
Size: 3605
BstYI r'GATC_y
Cuts at: 1 630 1097 1120 1455 1701 2713 2799 2811
Size: 629 467 23 335 246 1012 86 12
Cuts at: 2811 3157 1
Size: 346 449
Fragments arranged by size:
1012 629 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 344 344
Size: 3605
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 264 1555 1736 1899 264
Size: 1291 181 163 1970
Fragments arranged by size:
1970 1291 181 163
BtsI GCAGTG_nn'
Cuts at: 848 848
Size: 3605
DraI TTT'AAA
Cuts at: 604 604
Size: 3605
DrdI GACnn_nn'nnGTC
Cuts at: 1311 2169 1311
Size: 858 2747
EaeI y'GGCC_r
Cuts at: 1190 1364 1755 1782 3127 1190
Size: 174 391 27 1345 1668
Fragments arranged by size:
1668 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1190 1190
Size: 3605
EarI CTCTTCn'nnn_
Cuts at: 175 1628 1838 175
Size: 1453 210 1942
Fragments arranged by size:
1942 1453 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1813 2123 2269 3415 1813
Size: 310 146 1146 2003
Fragments arranged by size:
2003 1146 310 146
EcoICRI GAG'CTC
Cuts at: 617 617
Size: 3605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 509 530 1430 1862 2609 2877 304
Size: 205 21 900 432 747 268 1032
Fragments arranged by size:
1032 900 747 432 268 205 21
EcoNI CCTnn'n_nnAGG
Cuts at: 473 473
Size: 3605
EcoO109I rG'GnC_Cy
Cuts at: 315 349 350 494 744 315
Size: 34 1 144 250 3176
Fragments arranged by size:
3176 250 144 34 1
EcoRI G'AATT_C
Cuts at: 609 609
Size: 3605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1624 1624
Size: 3605
FspI TGC'GCA
Cuts at: 1386 1386
Size: 3605
HaeII r_GCGC'y
Cuts at: 1039 1287 2309 3288 1039
Size: 248 1022 979 1356
Fragments arranged by size:
1356 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1447 1477 1479 1509 1447
Size: 30 2 30 3543
Fragments arranged by size:
3543 30 30 2
HincII GTy'rAC
Cuts at: 644 644
Size: 3605
KasI G'GCGC_C
Cuts at: 1035 1283 1035
Size: 248 3357
KpnI G_GTAC'C
Cuts at: 625 625
Size: 3605
MluI A'CGCG_T
Cuts at: 3038 3038
Size: 3605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2276 2460 2276
Size: 184 3421
MscI TGG'CCA
Cuts at: 1366 1366
Size: 3605
MslI CAynn'nnrTG
Cuts at: 1594 1721 1594
Size: 127 3478
MspA1I CmG'CkG
Cuts at: 195 716 1030 1390 2403 2648 195
Size: 521 314 360 1013 245 1152
Fragments arranged by size:
1152 1013 521 360 314 245
NaeI GCC'GGC
Cuts at: 574 1786 574
Size: 1212 2393
NarI GG'CG_CC
Cuts at: 1036 1284 1036
Size: 248 3357
NgoMIV G'CCGG_C
Cuts at: 572 1784 572
Size: 1212 2393
NspI r_CATG'y
Cuts at: 658 1158 2065 658
Size: 500 907 2198
Fragments arranged by size:
2198 907 500
PciI A'CATG_T
Cuts at: 2061 2061
Size: 3605
PfoI T'CCnGG_A
Cuts at: 233 233
Size: 3605
PmeI GTTT'AAAC
Cuts at: 604 604
Size: 3605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2744 2776 2744
Size: 32 3573
PpuMI rG'GwC_Cy
Cuts at: 494 494
Size: 3605
PspOMI G'GGCC_C
Cuts at: 349 349
Size: 3605
PstI C_TGCA'G
Cuts at: 416 652 1337 416
Size: 236 685 2684
Fragments arranged by size:
2684 685 236
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3605
PvuII CAG'CTG
Cuts at: 195 1030 1390 195
Size: 835 360 2410
Fragments arranged by size:
2410 835 360
RsrII CG'GwC_CG
Cuts at: 1800 1800
Size: 3605
SacI G_AGCT'C
Cuts at: 619 619
Size: 3605
SalI G'TCGA_C
Cuts at: 642 642
Size: 3605
SanDI GG'GwC_CC
Cuts at: 494 494
Size: 3605
SapI GCTCTTCn'nnn_
Cuts at: 1628 1838 1628
Size: 210 3395
SbfI CC_TGCA'GG
Cuts at: 652 652
Size: 3605
ScaI AGT'ACT
Cuts at: 3122 3122
Size: 3605
SfcI C'TryA_G
Cuts at: 33 412 648 1333 2326 2517 33
Size: 379 236 685 993 191 1121
Fragments arranged by size:
1121 993 685 379 236 191
SfoI GGC'GCC
Cuts at: 1037 1285 1037
Size: 248 3357
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3605
SmaI CCC'GGG
Cuts at: 627 627
Size: 3605
SmlI C'TyrA_G
Cuts at: 760 1675 2167 2429 760
Size: 915 492 262 1936
Fragments arranged by size:
1936 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3605
SphI G_CATG'C
Cuts at: 658 1158 658
Size: 500 3105
StuI AGG'CCT
Cuts at: 157 157
Size: 3605
StyI C'CwwG_G
Cuts at: 318 739 1093 318
Size: 421 354 2830
Fragments arranged by size:
2830 421 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1188 1321 1780 1989 1188
Size: 133 459 209 2804
Fragments arranged by size:
2804 459 209 133
TatI w'GTAC_w
Cuts at: 3120 3120
Size: 3605
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 3573
Tth111I GACn'n_nGTC
Cuts at: 265 1402 265
Size: 1137 2468
XbaI T'CTAG_A
Cuts at: 636 636
Size: 3605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1100 1100
Size: 3605
XmaI C'CCGG_G
Cuts at: 625 625
Size: 3605
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BsaXI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI