Restriction map for
pF1KB5561
(Circular) (Six-base) MAPSORT of: pF1KB5561.seq Check: 3338 from: 1 to: 4604
pF1KB5561 4604 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 1455 1560 1642 1455
Size: 105 82 4417
Fragments arranged by size:
4417 105 82
Acc65I G'GTAC_C
Cuts at: 1620 1620
Size: 4604
AclI AA'CG_TT
Cuts at: 4444 4444
Size: 4604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1139 1205 2429 2861 3608 3876 535
Size: 604 66 1224 432 747 268 1263
Fragments arranged by size:
1263 1224 747 604 432 268 66
AflIII A'CryG_T
Cuts at: 140 1513 1956 3060 4037 140
Size: 1373 443 1104 977 707
Fragments arranged by size:
1373 1104 977 707 443
AgeI A'CCGG_T
Cuts at: 2992 2992
Size: 4604
AlwNI CAG_nnn'CTG
Cuts at: 1909 3476 1909
Size: 1567 3037
ApaI G_GGCC'C
Cuts at: 1098 1098
Size: 4604
ApaLI G'TGCA_C
Cuts at: 251 3374 251
Size: 3123 1481
ApoI r'AATT_y
Cuts at: 636 868 1013 1608 3957 4213 636
Size: 232 145 595 2349 256 1027
Fragments arranged by size:
2349 1027 595 256 232 145
AseI AT'TA_AT
Cuts at: 20 20
Size: 4604
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
AvaI C'yCGr_G
Cuts at: 1624 1624
Size: 4604
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 473 506 473
Size: 33 4571
BamHI G'GATC_C
Cuts at: 1629 1629
Size: 4604
BanI G'GyrC_C
Cuts at: 1620 2034 2282 2317 1620
Size: 414 248 35 3907
Fragments arranged by size:
3907 414 248 35
BanII G_rGCy'C
Cuts at: 1098 1618 2648 1098
Size: 520 1030 3054
Fragments arranged by size:
3054 1030 520
BbeI G_GCGC'C
Cuts at: 2038 2286 2038
Size: 248 4356
BbsI GAAGACnn'nnnn_
Cuts at: 1307 1307
Size: 4604
BciVI GTATCCnnnnn_n'
Cuts at: 2502 3263 4604 2502
Size: 761 1341 2502
Fragments arranged by size:
2502 1341 761
BclI T'GATC_A
Cuts at: 525 2124 525
Size: 1599 3005
BglII A'GATC_T
Cuts at: 2119 4156 2119
Size: 2037 2567
BlpI GC'TnA_GC
Cuts at: 1716 1716
Size: 4604
Bme1580I G_kGCm'C
Cuts at: 255 1098 2229 2322 3378 255
Size: 843 1131 93 1056 1481
Fragments arranged by size:
1481 1131 1056 843 93
BmrI ACTGGGnnnn_n'
Cuts at: 1546 1816 1897 2232 4376 1546
Size: 270 81 335 2144 1774
Fragments arranged by size:
2144 1774 335 270 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4604
Bpu10I CC'TnA_GC
Cuts at: 1020 1020
Size: 4604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1780 2659 3151 3449 1780
Size: 879 492 298 2935
Fragments arranged by size:
2935 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 193 4308 193
Size: 4115 489
BsaAI yAC'GTr
Cuts at: 1957 1957
Size: 4604
BsaBI GATnn'nnATC
Cuts at: 6 2118 4155 6
Size: 2112 2037 455
Fragments arranged by size:
2112 2037 455
BsaHI Gr'CG_yC
Cuts at: 778 2035 2283 2985 4481 778
Size: 1257 248 702 1496 901
Fragments arranged by size:
1496 1257 901 702 248
BsaWI w'CCGG_w
Cuts at: 789 1793 2314 2992 3266 3413 3824 789
Size: 1004 521 678 274 147 411 1569
Fragments arranged by size:
1569 1004 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 129 129
Size: 4604
BseYI C'CCAG_C
Cuts at: 2029 2787 3364 3860 2029
Size: 758 577 496 2773
Fragments arranged by size:
2773 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2146 2192 3400 104
Size: 2042 46 1208 1308
Fragments arranged by size:
2042 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 255 413 1618 2396 2586 3378 255
Size: 158 1205 778 190 792 1481
Fragments arranged by size:
1481 1205 792 778 190 158
Bsp1286I G_dGCh'C
Cuts at: 255 413 1098 1618 2229 2322 2396 2586 2648
Size: 158 685 520 611 93 74 190 62
Cuts at: 2648 3378 255
Size: 730 1481
Fragments arranged by size:
1481 730 685 611 520 190 158 93
74 62
BspEI T'CCGG_A
Cuts at: 789 3824 789
Size: 3035 1569
BspHI T'CATG_A
Cuts at: 3780 3780
Size: 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1654 1868 2170 2551 1654
Size: 214 302 381 3707
Fragments arranged by size:
3707 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2896 2950 4434 4599 50
Size: 2846 54 1484 165 55
Fragments arranged by size:
2846 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 220 1056 1077 2516 220
Size: 836 21 1439 2308
Fragments arranged by size:
2308 1439 836 21
BsrFI r'CCGG_y
Cuts at: 2602 2783 2992 2602
Size: 181 209 4214
Fragments arranged by size:
4214 209 181
BsrGI T'GTAC_A
Cuts at: 540 540
Size: 4604
BssSI C'ACGA_G
Cuts at: 2875 3233 2875
Size: 358 4246
BstAPI GCAn_nnn'nTGC
Cuts at: 1046 1981 1046
Size: 935 3669
BstBI TT'CG_AA
Cuts at: 2965 2965
Size: 4604
BstYI r'GATC_y
Cuts at: 1 792 1005 1629 2096 2119 2454 2700 3712
Size: 791 213 624 467 23 335 246 1012
Cuts at: 3712 3798 3810 4156 1
Size: 86 12 346 449
Fragments arranged by size:
1012 791 624 467 449 346 335 246
213 86 23 12
BtgI C'CryG_G
Cuts at: 107 1479 107
Size: 1372 3232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2554 2735 2898 2554
Size: 181 163 4260
Fragments arranged by size:
4260 181 163
BtsI GCAGTG_nn'
Cuts at: 768 1847 768
Size: 1079 3525
DraI TTT'AAA
Cuts at: 1603 1603
Size: 4604
DrdI GACnn_nn'nnGTC
Cuts at: 2310 3168 2310
Size: 858 3746
EaeI y'GGCC_r
Cuts at: 297 2189 2363 2754 2781 4126 297
Size: 1892 174 391 27 1345 775
Fragments arranged by size:
1892 1345 775 391 174 27
EagI C'GGCC_G
Cuts at: 2189 2189
Size: 4604
EarI CTCTTCn'nnn_
Cuts at: 551 1181 1388 2627 2837 551
Size: 630 207 1239 210 2318
Fragments arranged by size:
2318 1239 630 210 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2812 3122 3268 4414 2812
Size: 310 146 1146 3002
Fragments arranged by size:
3002 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1616 1616
Size: 4604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1139 1205 2429 2861 3608 3876 535
Size: 604 66 1224 432 747 268 1263
Fragments arranged by size:
1263 1224 747 604 432 268 66
EcoO109I rG'GnC_Cy
Cuts at: 534 1095 1743 534
Size: 561 648 3395
Fragments arranged by size:
3395 648 561
EcoRI G'AATT_C
Cuts at: 1608 1608
Size: 4604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 301 2623 301
Size: 2322 2282
FspI TGC'GCA
Cuts at: 2385 2385
Size: 4604
HaeII r_GCGC'y
Cuts at: 2038 2286 3308 4287 2038
Size: 248 1022 979 2355
Fragments arranged by size:
2355 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 634 666 911 943 2446 2476 2478 2508 634
Size: 32 245 32 1503 30 2 30 2730
Fragments arranged by size:
2730 1503 245 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1456 1643 1456
Size: 187 4417
HindIII A'AGCT_T
Cuts at: 393 1277 393
Size: 884 3720
KasI G'GCGC_C
Cuts at: 2034 2282 2034
Size: 248 4356
KpnI G_GTAC'C
Cuts at: 1624 1624
Size: 4604
MluI A'CGCG_T
Cuts at: 4037 4037
Size: 4604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 812 3275 3459 812
Size: 2463 184 1957
Fragments arranged by size:
2463 1957 184
MscI TGG'CCA
Cuts at: 299 2365 299
Size: 2066 2538
MslI CAynn'nnrTG
Cuts at: 821 2593 2720 821
Size: 1772 127 2705
Fragments arranged by size:
2705 1772 127
MspA1I CmG'CkG
Cuts at: 1715 2029 2389 3402 3647 1715
Size: 314 360 1013 245 2672
Fragments arranged by size:
2672 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2785 2785
Size: 4604
NarI GG'CG_CC
Cuts at: 2035 2283 2035
Size: 248 4356
NcoI C'CATG_G
Cuts at: 107 1479 107
Size: 1372 3232
NgoMIV G'CCGG_C
Cuts at: 2783 2783
Size: 4604
NspI r_CATG'y
Cuts at: 144 1517 1657 2157 3064 144
Size: 1373 140 500 907 1684
Fragments arranged by size:
1684 1373 907 500 140
PciI A'CATG_T
Cuts at: 140 1513 3060 140
Size: 1373 1547 1684
Fragments arranged by size:
1684 1547 1373
PmeI GTTT'AAAC
Cuts at: 1603 1603
Size: 4604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 3743
Size: 32 4572
PpuMI rG'GwC_Cy
Cuts at: 534 534
Size: 4604
PspOMI G'GGCC_C
Cuts at: 1094 1094
Size: 4604
PstI C_TGCA'G
Cuts at: 1651 2336 1651
Size: 685 3919
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4604
PvuII CAG'CTG
Cuts at: 2029 2389 2029
Size: 360 4244
RsrII CG'GwC_CG
Cuts at: 2799 2799
Size: 4604
SacI G_AGCT'C
Cuts at: 1618 1618
Size: 4604
SalI G'TCGA_C
Cuts at: 1454 1641 1454
Size: 187 4417
SapI GCTCTTCn'nnn_
Cuts at: 2627 2837 2627
Size: 210 4394
SbfI CC_TGCA'GG
Cuts at: 1651 1651
Size: 4604
ScaI AGT'ACT
Cuts at: 4121 4121
Size: 4604
SfcI C'TryA_G
Cuts at: 33 435 770 1220 1647 2332 3325 3516 33
Size: 402 335 450 427 685 993 191 1121
Fragments arranged by size:
1121 993 685 450 427 402 335 191
SfoI GGC'GCC
Cuts at: 2036 2284 2036
Size: 248 4356
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
SmaI CCC'GGG
Cuts at: 1626 1626
Size: 4604
SmlI C'TyrA_G
Cuts at: 1759 2674 3166 3428 1759
Size: 915 492 262 2935
Fragments arranged by size:
2935 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4604
SphI G_CATG'C
Cuts at: 1657 2157 1657
Size: 500 4104
StyI C'CwwG_G
Cuts at: 107 1479 1738 2092 107
Size: 1372 259 354 2619
Fragments arranged by size:
2619 1372 354 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2187 2320 2779 2988 2187
Size: 133 459 209 3803
Fragments arranged by size:
3803 459 209 133
TatI w'GTAC_w
Cuts at: 540 4119 540
Size: 3579 1025
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4121 4153 4121
Size: 32 4572
Tth111I GACn'n_nGTC
Cuts at: 2401 2401
Size: 4604
XbaI T'CTAG_A
Cuts at: 586 604 1635 586
Size: 18 1031 3555
Fragments arranged by size:
3555 1031 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2099 2099
Size: 4604
XmaI C'CCGG_G
Cuts at: 1624 1624
Size: 4604
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI