Restriction map for
pF1KB5545
(Circular) (Six-base) MAPSORT of: pF1KB5545.seq Check: 3141 from: 1 to: 4985
pF1KB5545 4985 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1216 1216
Size: 4985
AccI GT'mk_AC
Cuts at: 1644 2023 1644
Size: 379 4606
Acc65I G'GTAC_C
Cuts at: 2001 2001
Size: 4985
AclI AA'CG_TT
Cuts at: 4825 4825
Size: 4985
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 716 992 2810 3242 3989 4257 356
Size: 360 276 1818 432 747 268 1084
Fragments arranged by size:
1818 1084 747 432 360 276 268
AflIII A'CryG_T
Cuts at: 2337 3441 4418 2337
Size: 1104 977 2904
Fragments arranged by size:
2904 1104 977
AgeI A'CCGG_T
Cuts at: 3373 3373
Size: 4985
AlwNI CAG_nnn'CTG
Cuts at: 973 2290 3857 973
Size: 1317 1567 2101
Fragments arranged by size:
2101 1567 1317
ApaI G_GGCC'C
Cuts at: 1656 1656
Size: 4985
ApaLI G'TGCA_C
Cuts at: 3755 3755
Size: 4985
ApoI r'AATT_y
Cuts at: 558 687 818 1256 1917 1989 4338 4594 558
Size: 129 131 438 661 72 2349 256 949
Fragments arranged by size:
2349 949 661 438 256 131 129 72
AseI AT'TA_AT
Cuts at: 20 20
Size: 4985
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4985
AvaI C'yCGr_G
Cuts at: 2005 2005
Size: 4985
BamHI G'GATC_C
Cuts at: 2010 2010
Size: 4985
BanI G'GyrC_C
Cuts at: 268 905 1627 2001 2415 2663 2698 268
Size: 637 722 374 414 248 35 2555
Fragments arranged by size:
2555 722 637 414 374 248 35
BanII G_rGCy'C
Cuts at: 754 1656 1999 3029 754
Size: 902 343 1030 2710
Fragments arranged by size:
2710 1030 902 343
BbeI G_GCGC'C
Cuts at: 2419 2667 2419
Size: 248 4737
BciVI GTATCCnnnnn_n'
Cuts at: 2883 3644 4985 2883
Size: 761 1341 2883
Fragments arranged by size:
2883 1341 761
BclI T'GATC_A
Cuts at: 144 2505 144
Size: 2361 2624
BfrBI ATG'CAT
Cuts at: 1804 1804
Size: 4985
BglII A'GATC_T
Cuts at: 829 2500 4537 829
Size: 1671 2037 1277
Fragments arranged by size:
2037 1671 1277
BlpI GC'TnA_GC
Cuts at: 2097 2097
Size: 4985
Bme1580I G_kGCm'C
Cuts at: 1656 2610 2703 3759 1656
Size: 954 93 1056 2882
Fragments arranged by size:
2882 1056 954 93
BmrI ACTGGGnnnn_n'
Cuts at: 1651 2197 2278 2613 4757 1651
Size: 546 81 335 2144 1879
Fragments arranged by size:
2144 1879 546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3352 3352
Size: 4985
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1118 1118
Size: 4985
Bpu10I CC'TnA_GC
Cuts at: 1693 1693
Size: 4985
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 977 2161 3040 3532 3830 977
Size: 1184 879 492 298 2132
Fragments arranged by size:
2132 1184 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1044 1151 4689 1044
Size: 107 3538 1340
Fragments arranged by size:
3538 1340 107
BsaAI yAC'GTr
Cuts at: 2338 2338
Size: 4985
BsaBI GATnn'nnATC
Cuts at: 6 651 2499 4536 6
Size: 645 1848 2037 455
Fragments arranged by size:
2037 1848 645 455
BsaHI Gr'CG_yC
Cuts at: 2416 2664 3366 4862 2416
Size: 248 702 1496 2539
Fragments arranged by size:
2539 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1549 2174 2695 3373 3647 3794 4205 1549
Size: 625 521 678 274 147 411 2329
Fragments arranged by size:
2329 678 625 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 593 623 593
Size: 30 4955
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 374 377 747 1202 1460 374
Size: 3 370 455 258 3899
Fragments arranged by size:
3899 455 370 258 3
BseYI C'CCAG_C
Cuts at: 2410 3168 3745 4241 2410
Size: 758 577 496 3154
Fragments arranged by size:
3154 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1902 2527 2573 3781 104
Size: 1798 625 46 1208 1308
Fragments arranged by size:
1798 1308 1208 625 46
BsiHKAI G_wGCw'C
Cuts at: 1999 2777 2967 3759 1999
Size: 778 190 792 3225
Fragments arranged by size:
3225 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 297 874 297
Size: 577 4408
Bsp1286I G_dGCh'C
Cuts at: 754 1656 1999 2610 2703 2777 2967 3029 3759
Size: 902 343 611 93 74 190 62 730
Cuts at: 3759 754
Size: 1980
Fragments arranged by size:
1980 902 730 611 343 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4205 4205
Size: 4985
BspHI T'CATG_A
Cuts at: 4161 4161
Size: 4985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 991 1216 2035 2249 2551 2932 991
Size: 225 819 214 302 381 3044
Fragments arranged by size:
3044 819 381 302 225 214
BsrBI CCG'CTC
Cuts at: 50 1221 3277 3331 4815 4980 50
Size: 1171 2056 54 1484 165 55
Fragments arranged by size:
2056 1484 1171 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1784 2897 1784
Size: 1113 3872
BsrFI r'CCGG_y
Cuts at: 2983 3164 3373 2983
Size: 181 209 4595
Fragments arranged by size:
4595 209 181
BsrGI T'GTAC_A
Cuts at: 1367 1367
Size: 4985
BssSI C'ACGA_G
Cuts at: 3256 3614 3256
Size: 358 4627
BstAPI GCAn_nnn'nTGC
Cuts at: 2362 2362
Size: 4985
BstBI TT'CG_AA
Cuts at: 3346 3346
Size: 4985
BstEII G'GTnAC_C
Cuts at: 794 794
Size: 4985
BstYI r'GATC_y
Cuts at: 1 829 2010 2477 2500 2835 3081 4093 4179
Size: 828 1181 467 23 335 246 1012 86
Cuts at: 4179 4191 4537 1
Size: 12 346 449
Fragments arranged by size:
1181 1012 828 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4985
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2935 3116 3279 2935
Size: 181 163 4641
Fragments arranged by size:
4641 181 163
BtsI GCAGTG_nn'
Cuts at: 485 1890 2228 485
Size: 1405 338 3242
Fragments arranged by size:
3242 1405 338
ClaI AT'CG_AT
Cuts at: 1898 1898
Size: 4985
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 120 155 120
Size: 35 4950
DraI TTT'AAA
Cuts at: 1984 1984
Size: 4985
DraIII CAC_nnn'GTG
Cuts at: 912 1438 912
Size: 526 4459
DrdI GACnn_nn'nnGTC
Cuts at: 2691 3549 2691
Size: 858 4127
EaeI y'GGCC_r
Cuts at: 2570 2744 3135 3162 4507 2570
Size: 174 391 27 1345 3048
Fragments arranged by size:
3048 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2570 2570
Size: 4985
EarI CTCTTCn'nnn_
Cuts at: 287 375 1080 1437 3008 3218 287
Size: 88 705 357 1571 210 2054
Fragments arranged by size:
2054 1571 705 357 210 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3193 3503 3649 4795 3193
Size: 310 146 1146 3383
Fragments arranged by size:
3383 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1997 1997
Size: 4985
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 716 992 1118 2810 3242 3989 4257 356
Size: 360 276 126 1692 432 747 268 1084
Fragments arranged by size:
1692 1084 747 432 360 276 268 126
EcoNI CCTnn'n_nnAGG
Cuts at: 722 722
Size: 4985
EcoO109I rG'GnC_Cy
Cuts at: 711 1652 1653 2124 711
Size: 941 1 471 3572
Fragments arranged by size:
3572 941 471 1
EcoRI G'AATT_C
Cuts at: 1256 1917 1989 1256
Size: 661 72 4252
Fragments arranged by size:
4252 661 72
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1004 1697 3004 1004
Size: 693 1307 2985
Fragments arranged by size:
2985 1307 693
FspI TGC'GCA
Cuts at: 2766 2766
Size: 4985
HaeII r_GCGC'y
Cuts at: 2419 2667 3689 4668 2419
Size: 248 1022 979 2736
Fragments arranged by size:
2736 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 367 399 1285 1317 1366 1398 2827 2857 2859
Size: 32 886 32 49 32 1429 30 2
Cuts at: 2859 2889 367
Size: 30 2463
Fragments arranged by size:
2463 1429 886 49 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 537 704 1645 2024 537
Size: 167 941 379 3498
Fragments arranged by size:
3498 941 379 167
HindIII A'AGCT_T
Cuts at: 197 197
Size: 4985
HpaI GTT'AAC
Cuts at: 537 704 537
Size: 167 4818
KasI G'GCGC_C
Cuts at: 2415 2663 2415
Size: 248 4737
KpnI G_GTAC'C
Cuts at: 2005 2005
Size: 4985
MluI A'CGCG_T
Cuts at: 4418 4418
Size: 4985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 466 1081 3656 3840 466
Size: 615 2575 184 1611
Fragments arranged by size:
2575 1611 615 184
MscI TGG'CCA
Cuts at: 2746 2746
Size: 4985
MslI CAynn'nnrTG
Cuts at: 2974 3101 2974
Size: 127 4858
MspA1I CmG'CkG
Cuts at: 1018 2096 2410 2770 3783 4028 1018
Size: 1078 314 360 1013 245 1975
Fragments arranged by size:
1975 1078 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3166 3166
Size: 4985
NarI GG'CG_CC
Cuts at: 2416 2664 2416
Size: 248 4737
NcoI C'CATG_G
Cuts at: 107 107
Size: 4985
NgoMIV G'CCGG_C
Cuts at: 3164 3164
Size: 4985
NsiI A_TGCA'T
Cuts at: 1806 1806
Size: 4985
NspI r_CATG'y
Cuts at: 2038 2538 3445 2038
Size: 500 907 3578
Fragments arranged by size:
3578 907 500
PciI A'CATG_T
Cuts at: 3441 3441
Size: 4985
PflMI CCAn_nnn'nTGG
Cuts at: 502 502
Size: 4985
PfoI T'CCnGG_A
Cuts at: 1095 1095
Size: 4985
PmeI GTTT'AAAC
Cuts at: 1984 1984
Size: 4985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1476 1494 1508 1526 4124 4156 1476
Size: 18 14 18 2598 32 2305
Fragments arranged by size:
2598 2305 32 18 18 14
PpuMI rG'GwC_Cy
Cuts at: 711 711
Size: 4985
PspOMI G'GGCC_C
Cuts at: 1652 1652
Size: 4985
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1073 1105 1458 1490 1073
Size: 32 353 32 4568
Fragments arranged by size:
4568 353 32 32
PstI C_TGCA'G
Cuts at: 156 2032 2717 156
Size: 1876 685 2424
Fragments arranged by size:
2424 1876 685
PvuI CG_AT'CG
Cuts at: 104 1902 104
Size: 1798 3187
PvuII CAG'CTG
Cuts at: 1018 2410 2770 1018
Size: 1392 360 3233
Fragments arranged by size:
3233 1392 360
RsrII CG'GwC_CG
Cuts at: 3180 3180
Size: 4985
SacI G_AGCT'C
Cuts at: 1999 1999
Size: 4985
SalI G'TCGA_C
Cuts at: 1643 2022 1643
Size: 379 4606
SapI GCTCTTCn'nnn_
Cuts at: 3008 3218 3008
Size: 210 4775
SbfI CC_TGCA'GG
Cuts at: 2032 2032
Size: 4985
ScaI AGT'ACT
Cuts at: 285 4502 285
Size: 4217 768
SfcI C'TryA_G
Cuts at: 33 152 310 1034 1815 2028 2713 3706 3897
Size: 119 158 724 781 213 685 993 191
Cuts at: 3897 33
Size: 1121
Fragments arranged by size:
1121 993 781 724 685 213 191 158
119
SfoI GGC'GCC
Cuts at: 2417 2665 2417
Size: 248 4737
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4985
SmaI CCC'GGG
Cuts at: 2007 2007
Size: 4985
SmlI C'TyrA_G
Cuts at: 992 2140 3055 3547 3809 992
Size: 1148 915 492 262 2168
Fragments arranged by size:
2168 1148 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4985
SphI G_CATG'C
Cuts at: 2038 2538 2038
Size: 500 4485
StyI C'CwwG_G
Cuts at: 107 2119 2473 107
Size: 2012 354 2619
Fragments arranged by size:
2619 2012 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 194 1127 2568 2701 3160 3369 194
Size: 933 1441 133 459 209 1810
Fragments arranged by size:
1810 1441 933 459 209 133
TatI w'GTAC_w
Cuts at: 283 1367 4500 283
Size: 1084 3133 768
Fragments arranged by size:
3133 1084 768
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 593 625 4502 4534 593
Size: 32 3877 32 1044
Fragments arranged by size:
3877 1044 32 32
Tth111I GACn'n_nGTC
Cuts at: 2782 2782
Size: 4985
XbaI T'CTAG_A
Cuts at: 673 895 2016 673
Size: 222 1121 3642
Fragments arranged by size:
3642 1121 222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2480 2480
Size: 4985
XmaI C'CCGG_G
Cuts at: 2005 2005
Size: 4985
XmnI GAAnn'nnTTC
Cuts at: 987 987
Size: 4985
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
BsgI BsiWI BsmI BssHII BstXI BstZ17I Bsu36I EcoRV
FseI FspAI MfeI NdeI NheI NotI NruI PacI
PasI PmlI PshAI PsiI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI