Restriction map for
pF1KB5542
(Circular) (Six-base) MAPSORT of: pF1KB5542.seq Check: 864 from: 1 to: 4187
pF1KB5542 4187 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1225 1225
Size: 4187
Acc65I G'GTAC_C
Cuts at: 1203 1203
Size: 4187
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 2444 3191 3459 2012
Size: 432 747 268 2740
Fragments arranged by size:
2740 747 432 268
AflIII A'CryG_T
Cuts at: 732 941 1539 2643 3620 732
Size: 209 598 1104 977 1299
Fragments arranged by size:
1299 1104 977 598 209
AgeI A'CCGG_T
Cuts at: 2575 2575
Size: 4187
AleI CACnn'nnGTG
Cuts at: 202 661 202
Size: 459 3728
AlwNI CAG_nnn'CTG
Cuts at: 1492 3059 1492
Size: 1567 2620
ApaI G_GGCC'C
Cuts at: 485 786 485
Size: 301 3886
ApaLI G'TGCA_C
Cuts at: 2957 2957
Size: 4187
ApoI r'AATT_y
Cuts at: 1191 3540 3796 1191
Size: 2349 256 1582
Fragments arranged by size:
2349 1582 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 970 1207 970
Size: 237 3950
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 306 315 915 1203 1617 1865 1900 306
Size: 9 600 288 414 248 35 2593
Fragments arranged by size:
2593 600 414 288 248 35 9
BanII G_rGCy'C
Cuts at: 175 230 485 722 786 860 977 1108 1201
Size: 55 255 237 64 74 117 131 93
Cuts at: 1201 2231 175
Size: 1030 2131
Fragments arranged by size:
2131 1030 255 237 131 117 93 74
64 55
BbeI G_GCGC'C
Cuts at: 319 1621 1869 319
Size: 1302 248 2637
Fragments arranged by size:
2637 1302 248
BbsI GAAGACnn'nnnn_
Cuts at: 659 659
Size: 4187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 288 322 288
Size: 34 4153
BciVI GTATCCnnnnn_n'
Cuts at: 2085 2846 4187 2085
Size: 761 1341 2085
Fragments arranged by size:
2085 1341 761
BclI T'GATC_A
Cuts at: 1707 1707
Size: 4187
BfrBI ATG'CAT
Cuts at: 465 465
Size: 4187
BglI GCCn_nnn'nGGC
Cuts at: 489 625 489
Size: 136 4051
BglII A'GATC_T
Cuts at: 593 1702 3739 593
Size: 1109 2037 1041
Fragments arranged by size:
2037 1109 1041
BlpI GC'TnA_GC
Cuts at: 1299 1299
Size: 4187
Bme1580I G_kGCm'C
Cuts at: 485 786 1158 1812 1905 2961 485
Size: 301 372 654 93 1056 1711
Fragments arranged by size:
1711 1056 654 372 301 93
BmrI ACTGGGnnnn_n'
Cuts at: 258 1399 1480 1815 3959 258
Size: 1141 81 335 2144 486
Fragments arranged by size:
2144 1141 486 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 736 944 1088 706
Size: 30 208 144 3805
Fragments arranged by size:
3805 208 144 30
Bpu10I CC'TnA_GC
Cuts at: 1107 1107
Size: 4187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1363 2242 2734 3032 1363
Size: 879 492 298 2518
Fragments arranged by size:
2518 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 449 859 1068 3891 449
Size: 410 209 2823 745
Fragments arranged by size:
2823 745 410 209
BsaAI yAC'GTr
Cuts at: 252 735 944 1540 252
Size: 483 209 596 2899
Fragments arranged by size:
2899 596 483 209
BsaBI GATnn'nnATC
Cuts at: 6 1701 3738 6
Size: 1695 2037 455
Fragments arranged by size:
2037 1695 455
BsaHI Gr'CG_yC
Cuts at: 287 316 1618 1866 2568 4064 287
Size: 29 1302 248 702 1496 410
Fragments arranged by size:
1496 1302 702 410 248 29
BsaWI w'CCGG_w
Cuts at: 1376 1897 2575 2849 2996 3407 1376
Size: 521 678 274 147 411 2156
Fragments arranged by size:
2156 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 547 577 1061 1091 1137 1167 547
Size: 30 484 30 46 30 3567
Fragments arranged by size:
3567 484 46 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 124 943 124
Size: 819 3368
BseYI C'CCAG_C
Cuts at: 477 779 1004 1612 2370 2947 3443 477
Size: 302 225 608 758 577 496 1221
Fragments arranged by size:
1221 758 608 577 496 302 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 973
Size: 4187
BsiEI CG_ry'CG
Cuts at: 104 219 1729 1775 2983 104
Size: 115 1510 46 1208 1308
Fragments arranged by size:
1510 1308 1208 115 46
BsiHKAI G_wGCw'C
Cuts at: 635 860 1108 1201 1979 2169 2961 635
Size: 225 248 93 778 190 792 1861
Fragments arranged by size:
1861 792 778 248 225 190 93
BsmBI CGTCTCn'nnnn_
Cuts at: 694 694
Size: 4187
Bsp1286I G_dGCh'C
Cuts at: 175 230 485 635 722 786 860 977 1108
Size: 55 255 150 87 64 74 117 131
Cuts at: 1108 1158 1201 1812 1905 1979 2169 2231 2961
Size: 50 43 611 93 74 190 62 730
Cuts at: 2961 175
Size: 1401
Fragments arranged by size:
1401 730 611 255 190 150 131 117
93 87 74 74 64 62 55 50
43
BspEI T'CCGG_A
Cuts at: 3407 3407
Size: 4187
BspHI T'CATG_A
Cuts at: 3363 3363
Size: 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 410 566 1237 1451 1753 2134 410
Size: 156 671 214 302 381 2463
Fragments arranged by size:
2463 671 381 302 214 156
BsrBI CCG'CTC
Cuts at: 50 2479 2533 4017 4182 50
Size: 2429 54 1484 165 55
Fragments arranged by size:
2429 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1031 2099 1031
Size: 1068 3119
BsrFI r'CCGG_y
Cuts at: 996 2185 2366 2575 996
Size: 1189 181 209 2608
Fragments arranged by size:
2608 1189 209 181
BssSI C'ACGA_G
Cuts at: 859 2458 2816 859
Size: 1599 358 2230
Fragments arranged by size:
2230 1599 358
BstAPI GCAn_nnn'nTGC
Cuts at: 462 1564 462
Size: 1102 3085
BstBI TT'CG_AA
Cuts at: 2548 2548
Size: 4187
BstYI r'GATC_y
Cuts at: 1 593 1054 1212 1679 1702 2037 2283 3295
Size: 592 461 158 467 23 335 246 1012
Cuts at: 3295 3381 3393 3739 1
Size: 86 12 346 449
Fragments arranged by size:
1012 592 467 461 449 346 335 246
158 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 772 772
Size: 4187
BtgI C'CryG_G
Cuts at: 107 219 310 319 501 572 107
Size: 112 91 9 182 71 3722
Fragments arranged by size:
3722 182 112 91 71 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2137 2318 2481 2137
Size: 181 163 3843
Fragments arranged by size:
3843 181 163
BtsI GCAGTG_nn'
Cuts at: 463 1430 463
Size: 967 3220
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 1893 2751 1893
Size: 858 3329
EaeI y'GGCC_r
Cuts at: 216 1772 1946 2337 2364 3709 216
Size: 1556 174 391 27 1345 694
Fragments arranged by size:
1556 1345 694 391 174 27
EagI C'GGCC_G
Cuts at: 216 1772 216
Size: 1556 2631
EarI CTCTTCn'nnn_
Cuts at: 1096 2210 2420 1096
Size: 1114 210 2863
Fragments arranged by size:
2863 1114 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 643 2395 2705 2851 3997 643
Size: 1752 310 146 1146 833
Fragments arranged by size:
1752 1146 833 310 146
EcoICRI GAG'CTC
Cuts at: 858 1106 1199 858
Size: 248 93 3846
Fragments arranged by size:
3846 248 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 736 944 1088 2012 2444 3191 3459 706
Size: 30 208 144 924 432 747 268 1434
Fragments arranged by size:
1434 924 747 432 268 208 144 30
EcoNI CCTnn'n_nnAGG
Cuts at: 139 550 688 139
Size: 411 138 3638
Fragments arranged by size:
3638 411 138
EcoO109I rG'GnC_Cy
Cuts at: 144 481 555 783 1326 144
Size: 337 74 228 543 3005
Fragments arranged by size:
3005 543 337 228 74
EcoRI G'AATT_C
Cuts at: 1191 1191
Size: 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 4187
FspI TGC'GCA
Cuts at: 1968 1968
Size: 4187
HaeII r_GCGC'y
Cuts at: 319 371 495 1621 1869 2891 3870 319
Size: 52 124 1126 248 1022 979 636
Fragments arranged by size:
1126 1022 979 636 248 124 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 427 459 547 579 592 624 1046 1078 2029
Size: 32 88 32 13 32 422 32 951
Cuts at: 2029 2059 2061 2091 427
Size: 30 2 30 2523
Fragments arranged by size:
2523 951 422 88 32 32 32 32
30 30 13 2
HincII GTy'rAC
Cuts at: 1226 1226
Size: 4187
KasI G'GCGC_C
Cuts at: 315 1617 1865 315
Size: 1302 248 2637
Fragments arranged by size:
2637 1302 248
KpnI G_GTAC'C
Cuts at: 1207 1207
Size: 4187
MluI A'CGCG_T
Cuts at: 3620 3620
Size: 4187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2858 3042 2858
Size: 184 4003
MscI TGG'CCA
Cuts at: 1948 1948
Size: 4187
MslI CAynn'nnrTG
Cuts at: 202 241 661 2176 2303 202
Size: 39 420 1515 127 2086
Fragments arranged by size:
2086 1515 420 127 39
MspA1I CmG'CkG
Cuts at: 221 312 503 574 907 1298 1612 1972 2985
Size: 91 191 71 333 391 314 360 1013
Cuts at: 2985 3230 221
Size: 245 1178
Fragments arranged by size:
1178 1013 391 360 333 314 245 191
91 71
NaeI GCC'GGC
Cuts at: 998 2368 998
Size: 1370 2817
NarI GG'CG_CC
Cuts at: 316 1618 1866 316
Size: 1302 248 2637
Fragments arranged by size:
2637 1302 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4187
NgoMIV G'CCGG_C
Cuts at: 996 2366 996
Size: 1370 2817
NsiI A_TGCA'T
Cuts at: 467 467
Size: 4187
NspI r_CATG'y
Cuts at: 662 1240 1740 2647 662
Size: 578 500 907 2202
Fragments arranged by size:
2202 907 578 500
PasI CC'CwG_GG
Cuts at: 786 1158 786
Size: 372 3815
PciI A'CATG_T
Cuts at: 2643 2643
Size: 4187
PfoI T'CCnGG_A
Cuts at: 565 565
Size: 4187
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PmlI CAC'GTG
Cuts at: 735 944 735
Size: 209 3978
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 4155
PpuMI rG'GwC_Cy
Cuts at: 555 555
Size: 4187
PspOMI G'GGCC_C
Cuts at: 481 782 481
Size: 301 3886
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 499 531 499
Size: 32 4155
PstI C_TGCA'G
Cuts at: 458 879 1234 1919 458
Size: 421 355 685 2726
Fragments arranged by size:
2726 685 421 355
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4187
PvuII CAG'CTG
Cuts at: 907 1612 1972 907
Size: 705 360 3122
Fragments arranged by size:
3122 705 360
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 860 1108 1201 860
Size: 248 93 3846
Fragments arranged by size:
3846 248 93
SacII CC_GC'GG
Cuts at: 222 313 504 575 222
Size: 91 191 71 3834
Fragments arranged by size:
3834 191 91 71
SalI G'TCGA_C
Cuts at: 1224 1224
Size: 4187
SapI GCTCTTCn'nnn_
Cuts at: 1096 2210 2420 1096
Size: 1114 210 2863
Fragments arranged by size:
2863 1114 210
SbfI CC_TGCA'GG
Cuts at: 879 1234 879
Size: 355 3832
ScaI AGT'ACT
Cuts at: 3704 3704
Size: 4187
SfcI C'TryA_G
Cuts at: 33 454 875 1230 1915 2908 3099 33
Size: 421 421 355 685 993 191 1121
Fragments arranged by size:
1121 993 685 421 421 355 191
SfoI GGC'GCC
Cuts at: 317 1619 1867 317
Size: 1302 248 2637
Fragments arranged by size:
2637 1302 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SmaI CCC'GGG
Cuts at: 1209 1209
Size: 4187
SmlI C'TyrA_G
Cuts at: 1342 2257 2749 3011 1342
Size: 915 492 262 2518
Fragments arranged by size:
2518 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4187
SphI G_CATG'C
Cuts at: 1240 1740 1240
Size: 500 3687
StuI AGG'CCT
Cuts at: 873 873
Size: 4187
StyI C'CwwG_G
Cuts at: 107 1050 1321 1675 107
Size: 943 271 354 2619
Fragments arranged by size:
2619 943 354 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 277 1770 1903 2362 2571 277
Size: 1493 133 459 209 1893
Fragments arranged by size:
1893 1493 459 209 133
TatI w'GTAC_w
Cuts at: 3702 3702
Size: 4187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 374 406 1137 1169 3704 3736 374
Size: 32 731 32 2535 32 825
Fragments arranged by size:
2535 825 731 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1984 1984
Size: 4187
XbaI T'CTAG_A
Cuts at: 1218 1218
Size: 4187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1020 1682 1020
Size: 662 3525
XmaI C'CCGG_G
Cuts at: 1207 1207
Size: 4187
XmnI GAAnn'nnTTC
Cuts at: 190 190
Size: 4187
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PflMI PshAI PsiI PspXI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI