Restriction map for
pF1KB5529
(Circular) (Six-base) MAPSORT of: pF1KB5529.seq Check: 4893 from: 1 to: 4013
pF1KB5529 4013 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 924 924
Size: 4013
AccI GT'mk_AC
Cuts at: 1051 1051
Size: 4013
Acc65I G'GTAC_C
Cuts at: 1029 1029
Size: 4013
AclI AA'CG_TT
Cuts at: 3853 3853
Size: 4013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1838 2270 3017 3285 460
Size: 1378 432 747 268 1188
Fragments arranged by size:
1378 1188 747 432 268
AflIII A'CryG_T
Cuts at: 1365 2469 3446 1365
Size: 1104 977 1932
Fragments arranged by size:
1932 1104 977
AgeI A'CCGG_T
Cuts at: 2401 2401
Size: 4013
AhdI GACnn_n'nnGTC
Cuts at: 234 234
Size: 4013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 347 445 760 925 347
Size: 98 315 165 3435
Fragments arranged by size:
3435 315 165 98
AlwNI CAG_nnn'CTG
Cuts at: 492 495 1318 2885 492
Size: 3 823 1567 1620
Fragments arranged by size:
1620 1567 823 3
ApaI G_GGCC'C
Cuts at: 291 416 708 291
Size: 125 292 3596
Fragments arranged by size:
3596 292 125
ApaLI G'TGCA_C
Cuts at: 430 2783 430
Size: 2353 1660
ApoI r'AATT_y
Cuts at: 1017 3366 3622 1017
Size: 2349 256 1408
Fragments arranged by size:
2349 1408 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
AvaI C'yCGr_G
Cuts at: 156 267 409 1033 156
Size: 111 142 624 3136
Fragments arranged by size:
3136 624 142 111
AvrII C'CTAG_G
Cuts at: 861 861
Size: 4013
BamHI G'GATC_C
Cuts at: 510 1038 510
Size: 528 3485
BanI G'GyrC_C
Cuts at: 183 1029 1443 1691 1726 183
Size: 846 414 248 35 2470
Fragments arranged by size:
2470 846 414 248 35
BanII G_rGCy'C
Cuts at: 163 291 416 708 1027 2057 163
Size: 128 125 292 319 1030 2119
Fragments arranged by size:
2119 1030 319 292 128 125
BbeI G_GCGC'C
Cuts at: 187 1447 1695 187
Size: 1260 248 2505
Fragments arranged by size:
2505 1260 248
BbvCI CC'TCA_GC
Cuts at: 206 379 841 206
Size: 173 462 3378
Fragments arranged by size:
3378 462 173
BciVI GTATCCnnnnn_n'
Cuts at: 1911 2672 4013 1911
Size: 761 1341 1911
Fragments arranged by size:
1911 1341 761
BclI T'GATC_A
Cuts at: 1533 1533
Size: 4013
BglI GCCn_nnn'nGGC
Cuts at: 269 402 269
Size: 133 3880
BglII A'GATC_T
Cuts at: 1528 3565 1528
Size: 2037 1976
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4013
Bme1580I G_kGCm'C
Cuts at: 291 416 434 708 1638 1731 2787 291
Size: 125 18 274 930 93 1056 1517
Fragments arranged by size:
1517 1056 930 274 125 93 18
BmgBI CAC'GTC
Cuts at: 315 315
Size: 4013
BmrI ACTGGGnnnn_n'
Cuts at: 285 786 1225 1306 1641 3785 285
Size: 501 439 81 335 2144 513
Fragments arranged by size:
2144 513 501 439 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 162 2380 162
Size: 2218 1795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 874 835
Size: 39 3974
Bpu10I CC'TnA_GC
Cuts at: 147 206 379 841 147
Size: 59 173 462 3319
Fragments arranged by size:
3319 462 173 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2068 2560 2858 1189
Size: 879 492 298 2344
Fragments arranged by size:
2344 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3717 3717
Size: 4013
BsaAI yAC'GTr
Cuts at: 1366 1366
Size: 4013
BsaBI GATnn'nnATC
Cuts at: 6 1527 3564 6
Size: 1521 2037 455
Fragments arranged by size:
2037 1521 455
BsaHI Gr'CG_yC
Cuts at: 184 277 1444 1692 2394 3890 184
Size: 93 1167 248 702 1496 307
Fragments arranged by size:
1496 1167 702 307 248 93
BsaWI w'CCGG_w
Cuts at: 1202 1723 2401 2675 2822 3233 1202
Size: 521 678 274 147 411 1982
Fragments arranged by size:
1982 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 157 187 157
Size: 30 3983
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 713 713
Size: 4013
BseYI C'CCAG_C
Cuts at: 243 630 1438 2196 2773 3269 243
Size: 387 808 758 577 496 987
Fragments arranged by size:
987 808 758 577 496 387
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 539 604 631 645 730 885 505
Size: 34 65 27 14 85 155 3633
Fragments arranged by size:
3633 155 85 65 34 27 14
BsiEI CG_ry'CG
Cuts at: 104 1555 1601 2809 104
Size: 1451 46 1208 1308
Fragments arranged by size:
1451 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 163 434 1027 1805 1995 2787 163
Size: 271 593 778 190 792 1389
Fragments arranged by size:
1389 792 778 593 271 190
Bsp1286I G_dGCh'C
Cuts at: 163 291 416 434 708 1027 1638 1731 1805
Size: 128 125 18 274 319 611 93 74
Cuts at: 1805 1995 2057 2787 163
Size: 190 62 730 1389
Fragments arranged by size:
1389 730 611 319 274 190 128 125
93 74 62 18
BspEI T'CCGG_A
Cuts at: 3233 3233
Size: 4013
BspHI T'CATG_A
Cuts at: 3189 3189
Size: 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 924 1063 1277 1579 1960 924
Size: 139 214 302 381 2977
Fragments arranged by size:
2977 381 302 214 139
BsrBI CCG'CTC
Cuts at: 50 2305 2359 3843 4008 50
Size: 2255 54 1484 165 55
Fragments arranged by size:
2255 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 570 576 1925 570
Size: 6 1349 2658
Fragments arranged by size:
2658 1349 6
BsrFI r'CCGG_y
Cuts at: 374 401 2011 2192 2401 374
Size: 27 1610 181 209 1986
Fragments arranged by size:
1986 1610 209 181 27
BssHII G'CGCG_C
Cuts at: 384 384
Size: 4013
BssSI C'ACGA_G
Cuts at: 2284 2642 2284
Size: 358 3655
BstAPI GCAn_nnn'nTGC
Cuts at: 492 1390 492
Size: 898 3115
BstBI TT'CG_AA
Cuts at: 2374 2374
Size: 4013
BstXI CCAn_nnnn'nTGG
Cuts at: 474 625 474
Size: 151 3862
BstYI r'GATC_y
Cuts at: 1 510 1038 1505 1528 1863 2109 3121 3207
Size: 509 528 467 23 335 246 1012 86
Cuts at: 3207 3219 3565 1
Size: 12 346 449
Fragments arranged by size:
1012 528 509 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 416 600 107
Size: 309 184 3520
Fragments arranged by size:
3520 309 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 2144 2307 1963
Size: 181 163 3669
Fragments arranged by size:
3669 181 163
BtsI GCAGTG_nn'
Cuts at: 643 688 1256 643
Size: 45 568 3400
Fragments arranged by size:
3400 568 45
DraI TTT'AAA
Cuts at: 1012 1012
Size: 4013
DrdI GACnn_nn'nnGTC
Cuts at: 1719 2577 1719
Size: 858 3155
EaeI y'GGCC_r
Cuts at: 372 458 752 959 1598 1772 2163 2190 3535
Size: 86 294 207 639 174 391 27 1345
Cuts at: 3535 372
Size: 850
Fragments arranged by size:
1345 850 639 391 294 207 174 86
27
EagI C'GGCC_G
Cuts at: 1598 1598
Size: 4013
EarI CTCTTCn'nnn_
Cuts at: 220 436 997 2036 2246 220
Size: 216 561 1039 210 1987
Fragments arranged by size:
1987 1039 561 216 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2221 2531 2677 3823 2221
Size: 310 146 1146 2411
Fragments arranged by size:
2411 1146 310 146
EcoICRI GAG'CTC
Cuts at: 161 1025 161
Size: 864 3149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 835 874 1838 2270 3017 3285 460
Size: 375 39 964 432 747 268 1188
Fragments arranged by size:
1188 964 747 432 375 268 39
EcoNI CCTnn'n_nnAGG
Cuts at: 778 778
Size: 4013
EcoO109I rG'GnC_Cy
Cuts at: 210 287 395 704 1152 210
Size: 77 108 309 448 3071
Fragments arranged by size:
3071 448 309 108 77
EcoRI G'AATT_C
Cuts at: 1017 1017
Size: 4013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 4013
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4013
HaeII r_GCGC'y
Cuts at: 187 861 1447 1695 2717 3696 187
Size: 674 586 248 1022 979 504
Fragments arranged by size:
1022 979 674 586 504 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 834 866 1855 1885 1887 1917 220
Size: 32 582 32 989 30 2 30 2316
Fragments arranged by size:
2316 989 582 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1052 1052
Size: 4013
KasI G'GCGC_C
Cuts at: 183 1443 1691 183
Size: 1260 248 2505
Fragments arranged by size:
2505 1260 248
KpnI G_GTAC'C
Cuts at: 1033 1033
Size: 4013
MluI A'CGCG_T
Cuts at: 3446 3446
Size: 4013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2684 2868 2684
Size: 184 3829
MscI TGG'CCA
Cuts at: 754 961 1774 754
Size: 207 813 2993
Fragments arranged by size:
2993 813 207
MslI CAynn'nnrTG
Cuts at: 835 2002 2129 835
Size: 1167 127 2719
Fragments arranged by size:
2719 1167 127
MspA1I CmG'CkG
Cuts at: 195 300 370 492 523 588 1124 1438 1798
Size: 105 70 122 31 65 536 314 360
Cuts at: 1798 2811 3056 195
Size: 1013 245 1152
Fragments arranged by size:
1152 1013 536 360 314 245 122 105
70 65 31
NaeI GCC'GGC
Cuts at: 376 2194 376
Size: 1818 2195
NarI GG'CG_CC
Cuts at: 184 1444 1692 184
Size: 1260 248 2505
Fragments arranged by size:
2505 1260 248
NcoI C'CATG_G
Cuts at: 107 600 107
Size: 493 3520
NdeI CA'TA_TG
Cuts at: 836 836
Size: 4013
NgoMIV G'CCGG_C
Cuts at: 374 2192 374
Size: 1818 2195
NspI r_CATG'y
Cuts at: 1066 1566 2473 1066
Size: 500 907 2606
Fragments arranged by size:
2606 907 500
PciI A'CATG_T
Cuts at: 2469 2469
Size: 4013
PfoI T'CCnGG_A
Cuts at: 593 593
Size: 4013
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 3981
PspOMI G'GGCC_C
Cuts at: 287 412 704 287
Size: 125 292 3596
Fragments arranged by size:
3596 292 125
PspXI vC'TCGA_Gb
Cuts at: 156 156
Size: 4013
PstI C_TGCA'G
Cuts at: 779 914 986 1060 1745 779
Size: 135 72 74 685 3047
Fragments arranged by size:
3047 685 135 74 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4013
PvuII CAG'CTG
Cuts at: 492 523 588 1438 1798 492
Size: 31 65 850 360 2707
Fragments arranged by size:
2707 850 360 65 31
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4013
SacI G_AGCT'C
Cuts at: 163 1027 163
Size: 864 3149
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4013
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
SbfI CC_TGCA'GG
Cuts at: 779 1060 779
Size: 281 3732
ScaI AGT'ACT
Cuts at: 3530 3530
Size: 4013
SfcI C'TryA_G
Cuts at: 33 775 910 982 1056 1741 2734 2925 33
Size: 742 135 72 74 685 993 191 1121
Fragments arranged by size:
1121 993 742 685 191 135 74 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 269 269
Size: 4013
SfoI GGC'GCC
Cuts at: 185 1445 1693 185
Size: 1260 248 2505
Fragments arranged by size:
2505 1260 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
SgrAI Cr'CCGG_yG
Cuts at: 401 401
Size: 4013
SmaI CCC'GGG
Cuts at: 1035 1035
Size: 4013
SmlI C'TyrA_G
Cuts at: 156 1168 2083 2575 2837 156
Size: 1012 915 492 262 1332
Fragments arranged by size:
1332 1012 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4013
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3513
StyI C'CwwG_G
Cuts at: 107 600 861 1147 1501 107
Size: 493 261 286 354 2619
Fragments arranged by size:
2619 493 354 286 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 740 1596 1729 2188 2397 740
Size: 856 133 459 209 2356
Fragments arranged by size:
2356 856 459 209 133
TatI w'GTAC_w
Cuts at: 3528 3528
Size: 4013
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 157 189 3530 3562 157
Size: 32 3341 32 608
Fragments arranged by size:
3341 608 32 32
Tth111I GACn'n_nGTC
Cuts at: 1810 1810
Size: 4013
XbaI T'CTAG_A
Cuts at: 1044 1044
Size: 4013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 933 1508 933
Size: 575 3438
XhoI C'TCGA_G
Cuts at: 156 156
Size: 4013
XmaI C'CCGG_G
Cuts at: 1033 1033
Size: 4013
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PfoI PmeI PpiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI BaeI BbsI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PsrI SacII
SanDI SexAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI