Restriction map for
pF1KB5527
(Circular) (Six-base) MAPSORT of: pF1KB5527.seq Check: 2097 from: 1 to: 5381
pF1KB5527 5381 bp
With 182 enzymes: SgfI *
July 31, 2008 16:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1328 1328
Size: 5381
AccI GT'mk_AC
Cuts at: 989 2419 989
Size: 1430 3951
Acc65I G'GTAC_C
Cuts at: 2397 2397
Size: 5381
AclI AA'CG_TT
Cuts at: 5221 5221
Size: 5381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 214 634 645 951 1106 1420 1438 1936
Size: 18 420 11 306 155 314 18 498
Cuts at: 1936 3206 3638 4385 4653 196
Size: 1270 432 747 268 924
Fragments arranged by size:
1270 924 747 498 432 420 314 306
268 155 18 18 11
AfeI AGC'GCT
Cuts at: 226 877 226
Size: 651 4730
AflIII A'CryG_T
Cuts at: 1868 2733 3837 4814 1868
Size: 865 1104 977 2435
Fragments arranged by size:
2435 1104 977 865
AgeI A'CCGG_T
Cuts at: 3769 3769
Size: 5381
AleI CACnn'nnGTG
Cuts at: 983 1480 983
Size: 497 4884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 298 1340 298
Size: 1042 4339
AlwNI CAG_nnn'CTG
Cuts at: 171 192 850 2028 2686 4253 171
Size: 21 658 1178 658 1567 1299
Fragments arranged by size:
1567 1299 1178 658 658 21
ApaI G_GGCC'C
Cuts at: 1076 1151 1339 1979 1076
Size: 75 188 640 4478
Fragments arranged by size:
4478 640 188 75
ApaLI G'TGCA_C
Cuts at: 4151 4151
Size: 5381
ApoI r'AATT_y
Cuts at: 1839 2052 2385 4734 4990 1839
Size: 213 333 2349 256 2230
Fragments arranged by size:
2349 2230 333 256 213
AseI AT'TA_AT
Cuts at: 20 20
Size: 5381
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5381
AvaI C'yCGr_G
Cuts at: 114 382 1432 2401 114
Size: 268 1050 969 3094
Fragments arranged by size:
3094 1050 969 268
BamHI G'GATC_C
Cuts at: 2406 2406
Size: 5381
BanI G'GyrC_C
Cuts at: 341 1316 1639 1742 1945 2397 2811 3059 3094
Size: 975 323 103 203 452 414 248 35
Cuts at: 3094 341
Size: 2628
Fragments arranged by size:
2628 975 452 414 323 248 203 103
35
BanII G_rGCy'C
Cuts at: 623 778 887 1076 1151 1339 1442 1583 1979
Size: 155 109 189 75 188 103 141 396
Cuts at: 1979 2395 3425 623
Size: 416 1030 2579
Fragments arranged by size:
2579 1030 416 396 189 188 155 141
109 103 75
BbeI G_GCGC'C
Cuts at: 2815 3063 2815
Size: 248 5133
BbsI GAAGACnn'nnnn_
Cuts at: 399 1499 399
Size: 1100 4281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 628 662 628
Size: 34 5347
BciVI GTATCCnnnnn_n'
Cuts at: 2287 3279 4040 5381 2287
Size: 992 761 1341 2287
Fragments arranged by size:
2287 1341 992 761
BclI T'GATC_A
Cuts at: 2901 2901
Size: 5381
BglI GCCn_nnn'nGGC
Cuts at: 1243 1243
Size: 5381
BglII A'GATC_T
Cuts at: 656 2360 2896 4933 656
Size: 1704 536 2037 1104
Fragments arranged by size:
2037 1704 1104 536
BlpI GC'TnA_GC
Cuts at: 1012 2493 1012
Size: 1481 3900
Bme1580I G_kGCm'C
Cuts at: 344 383 1076 1104 1151 1339 1692 1745 1979
Size: 39 693 28 47 188 353 53 234
Cuts at: 1979 1989 3006 3099 4155 344
Size: 10 1017 93 1056 1570
Fragments arranged by size:
1570 1056 1017 693 353 234 188 93
53 47 39 28 10
BmgBI CAC'GTC
Cuts at: 1869 2106 1869
Size: 237 5144
BmrI ACTGGGnnnn_n'
Cuts at: 477 2593 2674 3009 5153 477
Size: 2116 81 335 2144 705
Fragments arranged by size:
2144 2116 705 335 81
BmtI G_CTAG'C
Cuts at: 1069 1573 1069
Size: 504 4877
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3748 3748
Size: 5381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 715 1075 1147 1294 1312 1472 1614 2353
Size: 336 360 72 147 18 160 142 739
Cuts at: 2353 379
Size: 3407
Fragments arranged by size:
3407 739 360 336 160 147 142 72
18
Bpu10I CC'TnA_GC
Cuts at: 777 1216 1391 1564 1747 1970 777
Size: 439 175 173 183 223 4188
Fragments arranged by size:
4188 439 223 183 175 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2269 2557 3436 3928 4226 2269
Size: 288 879 492 298 3424
Fragments arranged by size:
3424 879 492 298 288
BsaI GGTCTCn'nnnn_
Cuts at: 1928 5085 1928
Size: 3157 2224
BsaAI yAC'GTr
Cuts at: 1911 2734 1911
Size: 823 4558
BsaBI GATnn'nnATC
Cuts at: 6 158 2895 4932 6
Size: 152 2737 2037 455
Fragments arranged by size:
2737 2037 455 152
BsaHI Gr'CG_yC
Cuts at: 1246 1669 2812 3060 3762 5258 1246
Size: 423 1143 248 702 1496 1369
Fragments arranged by size:
1496 1369 1143 702 423 248
BsaWI w'CCGG_w
Cuts at: 2570 3091 3769 4043 4190 4601 2570
Size: 521 678 274 147 411 3350
Fragments arranged by size:
3350 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 490 520 775 805 2158 2188 490
Size: 30 255 30 1353 30 3683
Fragments arranged by size:
3683 1353 255 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 231 718 850 1542 1773 2226 231
Size: 487 132 692 231 453 3386
Fragments arranged by size:
3386 692 487 453 231 132
BseYI C'CCAG_C
Cuts at: 299 461 474 734 886 1075 1139 2806 3564
Size: 162 13 260 152 189 64 1667 758
Cuts at: 3564 4141 4637 299
Size: 577 496 1043
Fragments arranged by size:
1667 1043 758 577 496 260 189 162
152 64 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 121 466 1269 1558 121
Size: 345 803 289 3944
Fragments arranged by size:
3944 803 345 289
BsiEI CG_ry'CG
Cuts at: 104 753 2923 2969 4177 104
Size: 649 2170 46 1208 1308
Fragments arranged by size:
2170 1308 1208 649 46
BsiHKAI G_wGCw'C
Cuts at: 623 1217 1583 2395 3173 3363 4155 623
Size: 594 366 812 778 190 792 1849
Fragments arranged by size:
1849 812 792 778 594 366 190
Bsp1286I G_dGCh'C
Cuts at: 344 383 623 778 887 1076 1104 1151 1217
Size: 39 240 155 109 189 28 47 66
Cuts at: 1217 1339 1442 1583 1692 1745 1979 1989 2395
Size: 122 103 141 109 53 234 10 406
Cuts at: 2395 3006 3099 3173 3363 3425 4155 344
Size: 611 93 74 190 62 730 1570
Fragments arranged by size:
1570 730 611 406 240 234 190 189
155 141 122 109 109 103 93 74
66 62 53 47 39 28 10
BspEI T'CCGG_A
Cuts at: 4601 4601
Size: 5381
BspHI T'CATG_A
Cuts at: 4557 4557
Size: 5381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1058 1328 2431 2645 2947 3328 1058
Size: 270 1103 214 302 381 3111
Fragments arranged by size:
3111 1103 381 302 270 214
BsrBI CCG'CTC
Cuts at: 50 3673 3727 5211 5376 50
Size: 3623 54 1484 165 55
Fragments arranged by size:
3623 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3293 3293
Size: 5381
BsrFI r'CCGG_y
Cuts at: 3379 3560 3769 3379
Size: 181 209 4991
Fragments arranged by size:
4991 209 181
BssSI C'ACGA_G
Cuts at: 3652 4010 3652
Size: 358 5023
BstAPI GCAn_nnn'nTGC
Cuts at: 471 850 1115 2028 2758 471
Size: 379 265 913 730 3094
Fragments arranged by size:
3094 913 730 379 265
BstBI TT'CG_AA
Cuts at: 3742 3742
Size: 5381
BstEII G'GTnAC_C
Cuts at: 1368 1368
Size: 5381
BstXI CCAn_nnnn'nTGG
Cuts at: 1852 1852
Size: 5381
BstYI r'GATC_y
Cuts at: 1 159 656 1196 1409 2084 2360 2406 2873
Size: 158 497 540 213 675 276 46 467
Cuts at: 2873 2896 3231 3477 4489 4575 4587 4933 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 675 540 497 467 449 346 335
276 246 213 158 86 46 23 12
Bsu36I CC'TnA_GG
Cuts at: 1469 1469
Size: 5381
BtgI C'CryG_G
Cuts at: 107 509 770 1173 1799 2088 2152 107
Size: 402 261 403 626 289 64 3336
Fragments arranged by size:
3336 626 403 402 289 261 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 423 431 1223 3331 3512 3675 423
Size: 8 792 2108 181 163 2129
Fragments arranged by size:
2129 2108 792 181 163 8
BtsI GCAGTG_nn'
Cuts at: 1281 1529 2624 1281
Size: 248 1095 4038
Fragments arranged by size:
4038 1095 248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2334 2369 2334
Size: 35 5346
DraI TTT'AAA
Cuts at: 2380 2380
Size: 5381
DraIII CAC_nnn'GTG
Cuts at: 471 1737 471
Size: 1266 4115
DrdI GACnn_nn'nnGTC
Cuts at: 294 3087 3945 294
Size: 2793 858 1730
Fragments arranged by size:
2793 1730 858
EaeI y'GGCC_r
Cuts at: 2108 2256 2966 3140 3531 3558 4903 2108
Size: 148 710 174 391 27 1345 2586
Fragments arranged by size:
2586 1345 710 391 174 148 27
EagI C'GGCC_G
Cuts at: 2966 2966
Size: 5381
EarI CTCTTCn'nnn_
Cuts at: 1038 1207 3404 3614 1038
Size: 169 2197 210 2805
Fragments arranged by size:
2805 2197 210 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1717 3589 3899 4045 5191 1717
Size: 1872 310 146 1146 1907
Fragments arranged by size:
1907 1872 1146 310 146
EcoICRI GAG'CTC
Cuts at: 621 1581 2393 621
Size: 960 812 3609
Fragments arranged by size:
3609 960 812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 214 379 634 645 715 951 1075 1106
Size: 18 165 255 11 70 236 124 31
Cuts at: 1106 1147 1294 1312 1420 1438 1472 1614 1936
Size: 41 147 18 108 18 34 142 322
Cuts at: 1936 2353 3206 3638 4385 4653 196
Size: 417 853 432 747 268 924
Fragments arranged by size:
924 853 747 432 417 322 268 255
236 165 147 142 124 108 70 41
34 31 18 18 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 1054 1417 1969 1054
Size: 363 552 4466
Fragments arranged by size:
4466 552 363
EcoO109I rG'GnC_Cy
Cuts at: 847 1336 1898 1975 1976 2520 847
Size: 489 562 77 1 544 3708
Fragments arranged by size:
3708 562 544 489 77 1
EcoRI G'AATT_C
Cuts at: 2052 2385 2052
Size: 333 5048
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 606 945 1218 1941 2265 3400 606
Size: 339 273 723 324 1135 2587
Fragments arranged by size:
2587 1135 723 339 324 273
FspI TGC'GCA
Cuts at: 3162 3162
Size: 5381
HaeII r_GCGC'y
Cuts at: 172 228 879 965 2815 3063 4085 5064 172
Size: 56 651 86 1850 248 1022 979 489
Fragments arranged by size:
1850 1022 979 651 489 248 86 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 775 807 1654 1686 2221 2253 3223 3253 3255
Size: 32 847 32 535 32 970 30 2
Cuts at: 3255 3285 775
Size: 30 2871
Fragments arranged by size:
2871 970 847 535 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2040 2420 2040
Size: 380 5001
KasI G'GCGC_C
Cuts at: 2811 3059 2811
Size: 248 5133
KpnI G_GTAC'C
Cuts at: 2401 2401
Size: 5381
MfeI C'AATT_G
Cuts at: 2057 2057
Size: 5381
MluI A'CGCG_T
Cuts at: 4814 4814
Size: 5381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1811 4052 4236 1811
Size: 2241 184 2956
Fragments arranged by size:
2956 2241 184
MscI TGG'CCA
Cuts at: 2110 2258 3142 2110
Size: 148 884 4349
Fragments arranged by size:
4349 884 148
MslI CAynn'nnrTG
Cuts at: 631 983 1322 1480 3370 3497 631
Size: 352 339 158 1890 127 2515
Fragments arranged by size:
2515 1890 352 339 158 127
MspA1I CmG'CkG
Cuts at: 303 392 454 738 772 831 909 2028 2492
Size: 89 62 284 34 59 78 1119 464
Cuts at: 2492 2806 3166 4179 4424 303
Size: 314 360 1013 245 1260
Fragments arranged by size:
1260 1119 1013 464 360 314 284 245
89 78 62 59 34
NaeI GCC'GGC
Cuts at: 3562 3562
Size: 5381
NarI GG'CG_CC
Cuts at: 2812 3060 2812
Size: 248 5133
NcoI C'CATG_G
Cuts at: 107 509 2088 2152 107
Size: 402 1579 64 3336
Fragments arranged by size:
3336 1579 402 64
NgoMIV G'CCGG_C
Cuts at: 3560 3560
Size: 5381
NheI G'CTAG_C
Cuts at: 1065 1569 1065
Size: 504 4877
NspI r_CATG'y
Cuts at: 1327 2434 2934 3841 1327
Size: 1107 500 907 2867
Fragments arranged by size:
2867 1107 907 500
PciI A'CATG_T
Cuts at: 3837 3837
Size: 5381
PflMI CCAn_nnn'nTGG
Cuts at: 212 741 999 1023 1363 1737 1830 212
Size: 529 258 24 340 374 93 3763
Fragments arranged by size:
3763 529 374 340 258 93 24
PfoI T'CCnGG_A
Cuts at: 1226 1886 2369 1226
Size: 660 483 4238
Fragments arranged by size:
4238 660 483
PmeI GTTT'AAAC
Cuts at: 2380 2380
Size: 5381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4520 4552 4520
Size: 32 5349
PshAI GACnn'nnGTC
Cuts at: 518 2125 518
Size: 1607 3774
PspOMI G'GGCC_C
Cuts at: 1072 1147 1335 1975 1072
Size: 75 188 640 4478
Fragments arranged by size:
4478 640 188 75
PstI C_TGCA'G
Cuts at: 605 821 2428 3113 605
Size: 216 1607 685 2873
Fragments arranged by size:
2873 1607 685 216
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5381
PvuII CAG'CTG
Cuts at: 303 454 2028 2806 3166 303
Size: 151 1574 778 360 2518
Fragments arranged by size:
2518 1574 778 360 151
RsrII CG'GwC_CG
Cuts at: 3576 3576
Size: 5381
SacI G_AGCT'C
Cuts at: 623 1583 2395 623
Size: 960 812 3609
Fragments arranged by size:
3609 960 812
SacII CC_GC'GG
Cuts at: 773 773
Size: 5381
SalI G'TCGA_C
Cuts at: 2418 2418
Size: 5381
SapI GCTCTTCn'nnn_
Cuts at: 3404 3614 3404
Size: 210 5171
SbfI CC_TGCA'GG
Cuts at: 2428 2428
Size: 5381
ScaI AGT'ACT
Cuts at: 4898 4898
Size: 5381
SexAI A'CCwGG_T
Cuts at: 1022 1022
Size: 5381
SfcI C'TryA_G
Cuts at: 33 145 163 529 601 817 1791 2424 3109
Size: 112 18 366 72 216 974 633 685
Cuts at: 3109 4102 4293 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 974 685 633 366 216 191
112 72 18
SfoI GGC'GCC
Cuts at: 2813 3061 2813
Size: 248 5133
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5381
SmaI CCC'GGG
Cuts at: 2403 2403
Size: 5381
SmlI C'TyrA_G
Cuts at: 2284 2536 3451 3943 4205 2284
Size: 252 915 492 262 3460
Fragments arranged by size:
3460 915 492 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5381
SphI G_CATG'C
Cuts at: 1327 2434 2934 1327
Size: 1107 500 3774
Fragments arranged by size:
3774 1107 500
StuI AGG'CCT
Cuts at: 715 1654 715
Size: 939 4442
StyI C'CwwG_G
Cuts at: 107 509 899 920 1550 2088 2152 2515 2869
Size: 402 390 21 630 538 64 363 354
Cuts at: 2869 107
Size: 2619
Fragments arranged by size:
2619 630 538 402 390 363 354 64
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 459 2964 3097 3556 3765 459
Size: 2505 133 459 209 2075
Fragments arranged by size:
2505 2075 459 209 133
TatI w'GTAC_w
Cuts at: 938 4896 938
Size: 3958 1423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 4898 4930 490
Size: 32 4376 32 941
Fragments arranged by size:
4376 941 32 32
Tth111I GACn'n_nGTC
Cuts at: 789 1591 3178 789
Size: 802 1587 2992
Fragments arranged by size:
2992 1587 802
XbaI T'CTAG_A
Cuts at: 2412 2412
Size: 5381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2076 2876 2076
Size: 800 4581
XmaI C'CCGG_G
Cuts at: 2401 2401
Size: 5381
XmnI GAAnn'nnTTC
Cuts at: 658 2248 658
Size: 1590 3791
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PflMI PfoI PmeI PpiI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbvCI
BfrBI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI
EcoRV FseI FspAI HindIII HpaI NdeI NotI NruI
NsiI PacI PasI PmlI PpuMI PsiI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI