Restriction map for
pF1KB5520
(Circular) (Six-base) MAPSORT of: pF1KB5520.seq Check: 2725 from: 1 to: 4115
pF1KB5520 4115 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 994 994
Size: 4115
AccI GT'mk_AC
Cuts at: 1153 1153
Size: 4115
Acc65I G'GTAC_C
Cuts at: 1131 1131
Size: 4115
AclI AA'CG_TT
Cuts at: 3955 3955
Size: 4115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 390 1940 2372 3119 3387 211
Size: 179 1550 432 747 268 939
Fragments arranged by size:
1550 939 747 432 268 179
AflIII A'CryG_T
Cuts at: 1467 2571 3548 1467
Size: 1104 977 2034
Fragments arranged by size:
2034 1104 977
AgeI A'CCGG_T
Cuts at: 2503 2503
Size: 4115
AleI CACnn'nnGTG
Cuts at: 192 192
Size: 4115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 161 161
Size: 4115
AlwNI CAG_nnn'CTG
Cuts at: 1420 2987 1420
Size: 1567 2548
ApaI G_GGCC'C
Cuts at: 980 980
Size: 4115
ApaLI G'TGCA_C
Cuts at: 2885 2885
Size: 4115
ApoI r'AATT_y
Cuts at: 241 370 412 1119 3468 3724 241
Size: 129 42 707 2349 256 632
Fragments arranged by size:
2349 707 632 256 129 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
AvaI C'yCGr_G
Cuts at: 154 494 965 973 1135 154
Size: 340 471 8 162 3134
Fragments arranged by size:
3134 471 340 162 8
BamHI G'GATC_C
Cuts at: 1140 1140
Size: 4115
BanI G'GyrC_C
Cuts at: 969 1057 1131 1545 1793 1828 969
Size: 88 74 414 248 35 3256
Fragments arranged by size:
3256 414 248 88 74 35
BanII G_rGCy'C
Cuts at: 980 1129 2159 980
Size: 149 1030 2936
Fragments arranged by size:
2936 1030 149
BbeI G_GCGC'C
Cuts at: 1549 1797 1549
Size: 248 3867
BbsI GAAGACnn'nnnn_
Cuts at: 396 461 396
Size: 65 4050
BbvCI CC'TCA_GC
Cuts at: 824 824
Size: 4115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 796 830 796
Size: 34 4081
BciVI GTATCCnnnnn_n'
Cuts at: 2013 2774 4115 2013
Size: 761 1341 2013
Fragments arranged by size:
2013 1341 761
BclI T'GATC_A
Cuts at: 1635 1635
Size: 4115
BglI GCCn_nnn'nGGC
Cuts at: 897 897
Size: 4115
BglII A'GATC_T
Cuts at: 1630 3667 1630
Size: 2037 2078
BlpI GC'TnA_GC
Cuts at: 1227 1227
Size: 4115
Bme1580I G_kGCm'C
Cuts at: 972 980 1740 1833 2889 972
Size: 8 760 93 1056 2198
Fragments arranged by size:
2198 1056 760 93 8
BmgBI CAC'GTC
Cuts at: 641 641
Size: 4115
BmrI ACTGGGnnnn_n'
Cuts at: 1327 1408 1743 3887 1327
Size: 81 335 2144 1555
Fragments arranged by size:
2144 1555 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4115
Bpu10I CC'TnA_GC
Cuts at: 824 824
Size: 4115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 2170 2662 2960 1291
Size: 879 492 298 2446
Fragments arranged by size:
2446 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3819 3819
Size: 4115
BsaAI yAC'GTr
Cuts at: 1468 1468
Size: 4115
BsaBI GATnn'nnATC
Cuts at: 6 209 1629 3666 6
Size: 203 1420 2037 455
Fragments arranged by size:
2037 1420 455 203
BsaHI Gr'CG_yC
Cuts at: 1546 1794 2496 3992 1546
Size: 248 702 1496 1669
Fragments arranged by size:
1669 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1304 1825 2503 2777 2924 3335 1304
Size: 521 678 274 147 411 2084
Fragments arranged by size:
2084 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 350 353 841 955 958 350
Size: 3 488 114 3 3507
Fragments arranged by size:
3507 488 114 3 3
BseYI C'CCAG_C
Cuts at: 863 1540 2298 2875 3371 863
Size: 677 758 577 496 1607
Fragments arranged by size:
1607 758 677 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 157
Size: 4115
BsiEI CG_ry'CG
Cuts at: 104 1657 1703 2911 104
Size: 1553 46 1208 1308
Fragments arranged by size:
1553 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1129 1907 2097 2889 1129
Size: 778 190 792 2355
Fragments arranged by size:
2355 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 972 980 1129 1740 1833 1907 2097 2159 2889
Size: 8 149 611 93 74 190 62 730
Cuts at: 2889 972
Size: 2198
Fragments arranged by size:
2198 730 611 190 149 93 74 62
8
BspEI T'CCGG_A
Cuts at: 3335 3335
Size: 4115
BspHI T'CATG_A
Cuts at: 3291 3291
Size: 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 994 1165 1379 1681 2062 994
Size: 171 214 302 381 3047
Fragments arranged by size:
3047 381 302 214 171
BsrBI CCG'CTC
Cuts at: 50 2407 2461 3945 4110 50
Size: 2357 54 1484 165 55
Fragments arranged by size:
2357 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2027 2027
Size: 4115
BsrFI r'CCGG_y
Cuts at: 871 2113 2294 2503 871
Size: 1242 181 209 2483
Fragments arranged by size:
2483 1242 209 181
BssSI C'ACGA_G
Cuts at: 2386 2744 2386
Size: 358 3757
BstAPI GCAn_nnn'nTGC
Cuts at: 1492 1492
Size: 4115
BstBI TT'CG_AA
Cuts at: 2476 2476
Size: 4115
BstYI r'GATC_y
Cuts at: 1 1140 1607 1630 1965 2211 3223 3309 3321
Size: 1139 467 23 335 246 1012 86 12
Cuts at: 3321 3667 1
Size: 346 449
Fragments arranged by size:
1139 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1025 107
Size: 918 3197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2065 2246 2409 2065
Size: 181 163 3771
Fragments arranged by size:
3771 181 163
BtsI GCAGTG_nn'
Cuts at: 1358 1358
Size: 4115
ClaI AT'CG_AT
Cuts at: 676 676
Size: 4115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 627 662 627
Size: 35 4080
DraI TTT'AAA
Cuts at: 1114 1114
Size: 4115
DrdI GACnn_nn'nnGTC
Cuts at: 1821 2679 1821
Size: 858 3257
EaeI y'GGCC_r
Cuts at: 771 803 1700 1874 2265 2292 3637 771
Size: 32 897 174 391 27 1345 1249
Fragments arranged by size:
1345 1249 897 391 174 32 27
EagI C'GGCC_G
Cuts at: 1700 1700
Size: 4115
EarI CTCTTCn'nnn_
Cuts at: 639 2138 2348 639
Size: 1499 210 2406
Fragments arranged by size:
2406 1499 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 868 2323 2633 2779 3925 868
Size: 1455 310 146 1146 1058
Fragments arranged by size:
1455 1146 1058 310 146
EcoICRI GAG'CTC
Cuts at: 1127 1127
Size: 4115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 390 1940 2372 3119 3387 211
Size: 179 1550 432 747 268 939
Fragments arranged by size:
1550 939 747 432 268 179
EcoNI CCTnn'n_nnAGG
Cuts at: 823 823
Size: 4115
EcoO109I rG'GnC_Cy
Cuts at: 429 490 618 977 984 1254 429
Size: 61 128 359 7 270 3290
Fragments arranged by size:
3290 359 270 128 61 7
EcoRI G'AATT_C
Cuts at: 1119 1119
Size: 4115
EcoRV GAT'ATC
Cuts at: 680 680
Size: 4115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 4115
FspI TGC'GCA
Cuts at: 1896 1896
Size: 4115
HaeII r_GCGC'y
Cuts at: 1549 1797 2819 3798 1549
Size: 248 1022 979 1866
Fragments arranged by size:
1866 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1957 1987 1989 2019 1957
Size: 30 2 30 4053
Fragments arranged by size:
4053 30 30 2
HincII GTy'rAC
Cuts at: 1154 1154
Size: 4115
KasI G'GCGC_C
Cuts at: 1545 1793 1545
Size: 248 3867
KpnI G_GTAC'C
Cuts at: 1135 1135
Size: 4115
MluI A'CGCG_T
Cuts at: 3548 3548
Size: 4115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 257 2786 2970 257
Size: 2529 184 1402
Fragments arranged by size:
2529 1402 184
MscI TGG'CCA
Cuts at: 773 1876 773
Size: 1103 3012
MslI CAynn'nnrTG
Cuts at: 192 2104 2231 192
Size: 1912 127 2076
Fragments arranged by size:
2076 1912 127
MspA1I CmG'CkG
Cuts at: 1226 1540 1900 2913 3158 1226
Size: 314 360 1013 245 2183
Fragments arranged by size:
2183 1013 360 314 245
NaeI GCC'GGC
Cuts at: 873 2296 873
Size: 1423 2692
NarI GG'CG_CC
Cuts at: 1546 1794 1546
Size: 248 3867
NcoI C'CATG_G
Cuts at: 107 107
Size: 4115
NdeI CA'TA_TG
Cuts at: 264 264
Size: 4115
NgoMIV G'CCGG_C
Cuts at: 871 2294 871
Size: 1423 2692
NspI r_CATG'y
Cuts at: 148 837 1168 1668 2575 148
Size: 689 331 500 907 1688
Fragments arranged by size:
1688 907 689 500 331
PasI CC'CwG_GG
Cuts at: 980 980
Size: 4115
PciI A'CATG_T
Cuts at: 2571 2571
Size: 4115
PmeI GTTT'AAAC
Cuts at: 1114 1114
Size: 4115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 4083
PpuMI rG'GwC_Cy
Cuts at: 429 490 984 429
Size: 61 494 3560
Fragments arranged by size:
3560 494 61
PspOMI G'GGCC_C
Cuts at: 976 976
Size: 4115
PstI C_TGCA'G
Cuts at: 1162 1847 1162
Size: 685 3430
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4115
PvuII CAG'CTG
Cuts at: 1540 1900 1540
Size: 360 3755
RsrII CG'GwC_CG
Cuts at: 2310 2310
Size: 4115
SacI G_AGCT'C
Cuts at: 1129 1129
Size: 4115
SalI G'TCGA_C
Cuts at: 1152 1152
Size: 4115
SanDI GG'GwC_CC
Cuts at: 429 490 984 429
Size: 61 494 3560
Fragments arranged by size:
3560 494 61
SapI GCTCTTCn'nnn_
Cuts at: 2138 2348 2138
Size: 210 3905
SbfI CC_TGCA'GG
Cuts at: 1162 1162
Size: 4115
ScaI AGT'ACT
Cuts at: 3632 3632
Size: 4115
SfcI C'TryA_G
Cuts at: 33 1158 1843 2836 3027 33
Size: 1125 685 993 191 1121
Fragments arranged by size:
1125 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1547 1795 1547
Size: 248 3867
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
SmaI CCC'GGG
Cuts at: 975 1137 975
Size: 162 3953
SmlI C'TyrA_G
Cuts at: 1270 2185 2677 2939 1270
Size: 915 492 262 2446
Fragments arranged by size:
2446 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4115
SphI G_CATG'C
Cuts at: 837 1168 1668 837
Size: 331 500 3284
Fragments arranged by size:
3284 500 331
StyI C'CwwG_G
Cuts at: 107 1249 1603 107
Size: 1142 354 2619
Fragments arranged by size:
2619 1142 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 974 1045 1698 1831 2290 2499 974
Size: 71 653 133 459 209 2590
Fragments arranged by size:
2590 653 459 209 133 71
TatI w'GTAC_w
Cuts at: 3630 3630
Size: 4115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 4083
Tth111I GACn'n_nGTC
Cuts at: 519 1912 519
Size: 1393 2722
XbaI T'CTAG_A
Cuts at: 1146 1146
Size: 4115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1610 1610
Size: 4115
XmaI C'CCGG_G
Cuts at: 973 1135 973
Size: 162 3953
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BfrBI BmtI BpmI BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I DraIII FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI