Restriction map for
pF1KB5497
(Circular) (Six-base) MAPSORT of: pF1KB5497.seq Check: 6161 from: 1 to: 4550
pF1KB5497 4550 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1588 1588
Size: 4550
Acc65I G'GTAC_C
Cuts at: 1566 1566
Size: 4550
AclI AA'CG_TT
Cuts at: 4390 4390
Size: 4550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 1470 2375 2807 3160 3822 175
Size: 1295 905 432 353 662 903
Fragments arranged by size:
1295 905 903 662 432 353
AflIII A'CryG_T
Cuts at: 1902 3702 3983 1902
Size: 1800 281 2469
Fragments arranged by size:
2469 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 378 405 1855 3293 378
Size: 27 1450 1438 1635
Fragments arranged by size:
1635 1450 1438 27
ApaLI G'TGCA_C
Cuts at: 3388 3388
Size: 4550
ApoI r'AATT_y
Cuts at: 1100 1146 1184 1352 1554 3903 4159 1100
Size: 46 38 168 202 2349 256 1491
Fragments arranged by size:
2349 1491 256 202 168 46 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 4550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
AvaI C'yCGr_G
Cuts at: 302 352 434 1570 302
Size: 50 82 1136 3282
Fragments arranged by size:
3282 1136 82 50
BamHI G'GATC_C
Cuts at: 1575 1575
Size: 4550
BanI G'GyrC_C
Cuts at: 1566 1980 2228 2263 1566
Size: 414 248 35 3853
Fragments arranged by size:
3853 414 248 35
BanII G_rGCy'C
Cuts at: 332 428 757 1205 1564 2594 332
Size: 96 329 448 359 1030 2288
Fragments arranged by size:
2288 1030 448 359 329 96
BbeI G_GCGC'C
Cuts at: 1984 2232 1984
Size: 248 4302
BciVI GTATCCnnnnn_n'
Cuts at: 1141 2448 3504 4550 1141
Size: 1307 1056 1046 1141
Fragments arranged by size:
1307 1141 1056 1046
BclI T'GATC_A
Cuts at: 407 2070 407
Size: 1663 2887
BglII A'GATC_T
Cuts at: 2065 4102 2065
Size: 2037 2513
BlpI GC'TnA_GC
Cuts at: 1424 1662 1424
Size: 238 4312
Bme1580I G_kGCm'C
Cuts at: 132 2175 2268 3392 132
Size: 2043 93 1124 1290
Fragments arranged by size:
2043 1290 1124 93
BmgBI CAC'GTC
Cuts at: 471 471
Size: 4550
BmrI ACTGGGnnnn_n'
Cuts at: 1762 1843 2178 4322 1762
Size: 81 335 2144 1990
Fragments arranged by size:
2144 1990 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 412 415 505 1130 1382 400
Size: 12 3 90 625 252 3568
Fragments arranged by size:
3568 625 252 90 12 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1726 2605 3319 3617 1726
Size: 879 714 298 2659
Fragments arranged by size:
2659 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 283 349 4254 283
Size: 66 3905 579
Fragments arranged by size:
3905 579 66
BsaAI yAC'GTr
Cuts at: 1903 1903
Size: 4550
BsaBI GATnn'nnATC
Cuts at: 6 2064 4101 6
Size: 2058 2037 455
Fragments arranged by size:
2058 2037 455
BsaHI Gr'CG_yC
Cuts at: 1981 2229 2931 4427 1981
Size: 248 702 1496 2104
Fragments arranged by size:
2104 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1739 2260 2938 3349 3496 3770 1739
Size: 521 678 411 147 274 2519
Fragments arranged by size:
2519 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 241 271 241
Size: 30 4520
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 664 1002 1213 1240 580
Size: 84 338 211 27 3890
Fragments arranged by size:
3890 338 211 84 27
BseYI C'CCAG_C
Cuts at: 1975 2733 3398 3806 1975
Size: 758 665 408 2719
Fragments arranged by size:
2719 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 460
Size: 4550
BsiEI CG_ry'CG
Cuts at: 104 2092 2138 3368 104
Size: 1988 46 1230 1286
Fragments arranged by size:
1988 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 332 428 1564 2342 2532 3392 332
Size: 96 1136 778 190 860 1490
Fragments arranged by size:
1490 1136 860 778 190 96
BsmI GAATG_Cn'
Cuts at: 1080 1080
Size: 4550
Bsp1286I G_dGCh'C
Cuts at: 132 332 428 757 1205 1564 2175 2268 2342
Size: 200 96 329 448 359 611 93 74
Cuts at: 2342 2532 2594 3392 132
Size: 190 62 798 1290
Fragments arranged by size:
1290 798 611 448 359 329 200 190
96 93 74 62
BspHI T'CATG_A
Cuts at: 963 2982 963
Size: 2019 2531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 591 1600 1814 2116 2497 591
Size: 1009 214 302 381 2644
Fragments arranged by size:
2644 1009 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2842 2896 4380 4545 50
Size: 2792 54 1484 165 55
Fragments arranged by size:
2792 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1053 2462 1053
Size: 1409 3141
BsrFI r'CCGG_y
Cuts at: 2548 2729 2548
Size: 181 4369
BssSI C'ACGA_G
Cuts at: 2821 3529 2821
Size: 708 3842
BstAPI GCAn_nnn'nTGC
Cuts at: 1927 1927
Size: 4550
BstBI TT'CG_AA
Cuts at: 2911 2911
Size: 4550
BstYI r'GATC_y
Cuts at: 1 374 501 1575 2042 2065 2400 2646 2952
Size: 373 127 1074 467 23 335 246 306
Cuts at: 2952 2964 3050 4102 1
Size: 12 86 1052 449
Fragments arranged by size:
1074 1052 467 449 373 335 306 246
127 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 419 419
Size: 4550
BtgI C'CryG_G
Cuts at: 107 734 107
Size: 627 3923
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2500 2681 2844 2500
Size: 181 163 4206
Fragments arranged by size:
4206 181 163
BtsI GCAGTG_nn'
Cuts at: 1793 1793
Size: 4550
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1035 1070 1203 1238 1035
Size: 35 133 35 4347
Fragments arranged by size:
4347 133 35 35
DraI TTT'AAA
Cuts at: 1549 1549
Size: 4550
DrdI GACnn_nn'nnGTC
Cuts at: 2256 3600 2256
Size: 1344 3206
EaeI y'GGCC_r
Cuts at: 2135 2309 2700 2727 4072 2135
Size: 174 391 27 1345 2613
Fragments arranged by size:
2613 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2135 2135
Size: 4550
EarI CTCTTCn'nnn_
Cuts at: 281 696 2573 2783 281
Size: 415 1877 210 2048
Fragments arranged by size:
2048 1877 415 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2758 3500 3646 4360 2758
Size: 742 146 714 2948
Fragments arranged by size:
2948 742 714 146
EcoICRI GAG'CTC
Cuts at: 330 426 1562 330
Size: 96 1136 3318
Fragments arranged by size:
3318 1136 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 400 412 415 505 1130 1382 1470 2375
Size: 225 12 3 90 625 252 88 905
Cuts at: 2375 2807 3160 3822 175
Size: 432 353 662 903
Fragments arranged by size:
905 903 662 625 432 353 252 225
90 88 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 244 418 581 984 244
Size: 174 163 403 3810
Fragments arranged by size:
3810 403 174 163
EcoO109I rG'GnC_Cy
Cuts at: 298 1689 298
Size: 1391 3159
EcoRI G'AATT_C
Cuts at: 1100 1184 1352 1554 1100
Size: 84 168 202 4096
Fragments arranged by size:
4096 202 168 84
EcoRV GAT'ATC
Cuts at: 1522 1522
Size: 4550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 4550
FspI TGC'GCA
Cuts at: 2331 2331
Size: 4550
HaeII r_GCGC'y
Cuts at: 1984 2232 3462 4233 1984
Size: 248 1230 771 2301
Fragments arranged by size:
2301 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 171 178 203 210 561 593 897 929 2392
Size: 7 25 7 351 32 304 32 1463
Cuts at: 2392 2422 2424 2454 171
Size: 30 2 30 2267
Fragments arranged by size:
2267 1463 351 304 32 32 30 30
25 7 7 2
HincII GTy'rAC
Cuts at: 337 1589 337
Size: 1252 3298
HindIII A'AGCT_T
Cuts at: 673 1313 673
Size: 640 3910
KasI G'GCGC_C
Cuts at: 1980 2228 1980
Size: 248 4302
KpnI G_GTAC'C
Cuts at: 1570 1570
Size: 4550
MluI A'CGCG_T
Cuts at: 3983 3983
Size: 4550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1428 3309 3493 1428
Size: 1881 184 2485
Fragments arranged by size:
2485 1881 184
MscI TGG'CCA
Cuts at: 2311 2311
Size: 4550
MslI CAynn'nnrTG
Cuts at: 614 968 1025 2539 2666 614
Size: 354 57 1514 127 2498
Fragments arranged by size:
2498 1514 354 127 57
MspA1I CmG'CkG
Cuts at: 498 1661 1975 2335 3119 3364 498
Size: 1163 314 360 784 245 1684
Fragments arranged by size:
1684 1163 784 360 314 245
NaeI GCC'GGC
Cuts at: 2731 2731
Size: 4550
NarI GG'CG_CC
Cuts at: 1981 2229 1981
Size: 248 4302
NcoI C'CATG_G
Cuts at: 107 734 107
Size: 627 3923
NdeI CA'TA_TG
Cuts at: 1387 1387
Size: 4550
NgoMIV G'CCGG_C
Cuts at: 2729 2729
Size: 4550
NspI r_CATG'y
Cuts at: 859 1432 1603 2103 3706 859
Size: 573 171 500 1603 1703
Fragments arranged by size:
1703 1603 573 500 171
PciI A'CATG_T
Cuts at: 3702 3702
Size: 4550
PflMI CCAn_nnn'nTGG
Cuts at: 138 840 138
Size: 702 3848
PfoI T'CCnGG_A
Cuts at: 323 377 323
Size: 54 4496
PmeI GTTT'AAAC
Cuts at: 1549 1549
Size: 4550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 4518
PpuMI rG'GwC_Cy
Cuts at: 298 298
Size: 4550
PstI C_TGCA'G
Cuts at: 1597 2282 1597
Size: 685 3865
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4550
PvuII CAG'CTG
Cuts at: 498 1975 2335 498
Size: 1477 360 2713
Fragments arranged by size:
2713 1477 360
RsrII CG'GwC_CG
Cuts at: 2745 2745
Size: 4550
SacI G_AGCT'C
Cuts at: 332 428 1564 332
Size: 96 1136 3318
Fragments arranged by size:
3318 1136 96
SalI G'TCGA_C
Cuts at: 1587 1587
Size: 4550
SapI GCTCTTCn'nnn_
Cuts at: 2573 2783 2573
Size: 210 4340
SbfI CC_TGCA'GG
Cuts at: 1597 1597
Size: 4550
ScaI AGT'ACT
Cuts at: 4067 4067
Size: 4550
SfcI C'TryA_G
Cuts at: 33 346 1593 2278 3246 3437 33
Size: 313 1247 685 968 191 1146
Fragments arranged by size:
1247 1146 968 685 313 191
SfoI GGC'GCC
Cuts at: 1982 2230 1982
Size: 248 4302
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
SmaI CCC'GGG
Cuts at: 436 1572 436
Size: 1136 3414
SmlI C'TyrA_G
Cuts at: 302 1705 2620 3334 3596 302
Size: 1403 915 714 262 1256
Fragments arranged by size:
1403 1256 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4550
SphI G_CATG'C
Cuts at: 1603 2103 1603
Size: 500 4050
StuI AGG'CCT
Cuts at: 239 1082 1243 239
Size: 843 161 3546
Fragments arranged by size:
3546 843 161
StyI C'CwwG_G
Cuts at: 107 240 365 734 1684 2038 107
Size: 133 125 369 950 354 2619
Fragments arranged by size:
2619 950 369 354 133 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 467 1270 2133 2266 2725 2934 467
Size: 803 863 133 459 209 2083
Fragments arranged by size:
2083 863 803 459 209 133
TatI w'GTAC_w
Cuts at: 588 862 4065 588
Size: 274 3203 1073
Fragments arranged by size:
3203 1073 274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 241 273 509 541 4067 4099 241
Size: 32 236 32 3526 32 692
Fragments arranged by size:
3526 692 236 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2347 2347
Size: 4550
XbaI T'CTAG_A
Cuts at: 940 1581 940
Size: 641 3909
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2045 2045
Size: 4550
XhoI C'TCGA_G
Cuts at: 302 302
Size: 4550
XmaI C'CCGG_G
Cuts at: 434 1570 434
Size: 1136 3414
XmnI GAAnn'nnTTC
Cuts at: 1399 1399
Size: 4550
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BglI BmtI Bpu10I BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I ClaI DraIII FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI