Restriction map for
pF1KB5493
(Circular) (Six-base) MAPSORT of: pF1KB5493.seq Check: 5580 from: 1 to: 5837
pF1KB5493 5837 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 735 1642 735
Size: 907 4930
AccI GT'mk_AC
Cuts at: 2875 2875
Size: 5837
Acc65I G'GTAC_C
Cuts at: 2311 2853 2311
Size: 542 5295
AclI AA'CG_TT
Cuts at: 5677 5677
Size: 5837
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 362 445 917 1708 1948 3662 4094 4447
Size: 96 83 472 791 240 1714 432 353
Cuts at: 4447 5109 266
Size: 662 994
Fragments arranged by size:
1714 994 791 662 472 432 353 240
96 83
AfeI AGC'GCT
Cuts at: 329 329
Size: 5837
AflIII A'CryG_T
Cuts at: 3189 4989 5270 3189
Size: 1800 281 3756
Fragments arranged by size:
3756 1800 281
AleI CACnn'nnGTG
Cuts at: 667 1888 667
Size: 1221 4616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 810 810
Size: 5837
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1210 1242 2051 2083 1210
Size: 32 809 32 4964
Fragments arranged by size:
4964 809 32 32
AlwNI CAG_nnn'CTG
Cuts at: 240 624 1750 1915 1941 2409 2635 3142 4580
Size: 384 1126 165 26 468 226 507 1438
Cuts at: 4580 240
Size: 1497
Fragments arranged by size:
1497 1438 1126 507 468 384 226 165
26
ApaLI G'TGCA_C
Cuts at: 4675 4675
Size: 5837
ApoI r'AATT_y
Cuts at: 397 2242 2841 5190 5446 397
Size: 1845 599 2349 256 788
Fragments arranged by size:
2349 1845 788 599 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5837
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5837
AvaI C'yCGr_G
Cuts at: 1109 2857 1109
Size: 1748 4089
BamHI G'GATC_C
Cuts at: 2862 2862
Size: 5837
BanI G'GyrC_C
Cuts at: 132 1137 2311 2428 2853 3267 3515 3550 132
Size: 1005 1174 117 425 414 248 35 2419
Fragments arranged by size:
2419 1174 1005 425 414 248 117 35
BanII G_rGCy'C
Cuts at: 547 1133 1440 1757 2851 3881 547
Size: 586 307 317 1094 1030 2503
Fragments arranged by size:
2503 1094 1030 586 317 307
BbeI G_GCGC'C
Cuts at: 136 3271 3519 136
Size: 3135 248 2454
Fragments arranged by size:
3135 2454 248
BbsI GAAGACnn'nnnn_
Cuts at: 950 1994 2692 950
Size: 1044 698 4095
Fragments arranged by size:
4095 1044 698
BciVI GTATCCnnnnn_n'
Cuts at: 300 1755 2223 3735 4791 5837 300
Size: 1455 468 1512 1056 1046 300
Fragments arranged by size:
1512 1455 1056 1046 468 300
BclI T'GATC_A
Cuts at: 3357 3357
Size: 5837
BfrBI ATG'CAT
Cuts at: 781 781
Size: 5837
BglI GCCn_nnn'nGGC
Cuts at: 1828 2329 2675 1828
Size: 501 346 4990
Fragments arranged by size:
4990 501 346
BglII A'GATC_T
Cuts at: 3352 5389 3352
Size: 2037 3800
BlpI GC'TnA_GC
Cuts at: 2949 2949
Size: 5837
Bme1580I G_kGCm'C
Cuts at: 386 864 2431 3462 3555 4679 386
Size: 478 1567 1031 93 1124 1544
Fragments arranged by size:
1567 1544 1124 1031 478 93
BmrI ACTGGGnnnn_n'
Cuts at: 1801 2713 3049 3130 3465 5609 1801
Size: 912 336 81 335 2144 2029
Fragments arranged by size:
2144 2029 912 336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4204 4204
Size: 5837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 295 352 778 1042 1867 140
Size: 155 57 426 264 825 4110
Fragments arranged by size:
4110 825 426 264 155 57
Bpu10I CC'TnA_GC
Cuts at: 608 830 1104 2236 608
Size: 222 274 1132 4209
Fragments arranged by size:
4209 1132 274 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1741 3013 3892 4606 4904 511
Size: 1230 1272 879 714 298 1444
Fragments arranged by size:
1444 1272 1230 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1801 5541 1801
Size: 3740 2097
BsaAI yAC'GTr
Cuts at: 825 3190 825
Size: 2365 3472
BsaBI GATnn'nnATC
Cuts at: 6 2258 3351 5388 6
Size: 2252 1093 2037 455
Fragments arranged by size:
2252 2037 1093 455
BsaHI Gr'CG_yC
Cuts at: 133 3268 3516 4218 5714 133
Size: 3135 248 702 1496 256
Fragments arranged by size:
3135 1496 702 256 248
BsaWI w'CCGG_w
Cuts at: 1472 2165 3026 3547 4225 4636 4783 5057 1472
Size: 693 861 521 678 411 147 274 2252
Fragments arranged by size:
2252 861 693 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 469 499 505 535 1210 1240 1393 1423 1840
Size: 30 6 30 675 30 153 30 417
Cuts at: 1840 1870 1954 1984 2051 2081 469
Size: 30 84 30 67 30 4225
Fragments arranged by size:
4225 675 417 153 84 67 30 30
30 30 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 1057 1218 1401 2113 2626 2776 127
Size: 930 161 183 712 513 150 3188
Fragments arranged by size:
3188 930 712 513 183 161 150
BseYI C'CCAG_C
Cuts at: 146 3262 4020 4685 5093 146
Size: 3116 758 665 408 890
Fragments arranged by size:
3116 890 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 1355 1826 1954 2129 160
Size: 1195 471 128 175 3868
Fragments arranged by size:
3868 1195 471 175 128
BsiEI CG_ry'CG
Cuts at: 104 2460 3379 3425 4655 104
Size: 2356 919 46 1230 1286
Fragments arranged by size:
2356 1286 1230 919 46
BsiHKAI G_wGCw'C
Cuts at: 1440 1757 2052 2851 3629 3819 4679 1440
Size: 317 295 799 778 190 860 2598
Fragments arranged by size:
2598 860 799 778 317 295 190
BsmI GAATG_Cn'
Cuts at: 991 1257 2597 991
Size: 266 1340 4231
Fragments arranged by size:
4231 1340 266
Bsp1286I G_dGCh'C
Cuts at: 386 547 864 1133 1440 1757 2052 2431 2851
Size: 161 317 269 307 317 295 379 420
Cuts at: 2851 3462 3555 3629 3819 3881 4679 386
Size: 611 93 74 190 62 798 1544
Fragments arranged by size:
1544 798 611 420 379 317 317 307
295 269 190 161 93 74 62
BspEI T'CCGG_A
Cuts at: 1472 2165 1472
Size: 693 5144
BspHI T'CATG_A
Cuts at: 573 2250 4269 573
Size: 1677 2019 2141
Fragments arranged by size:
2141 2019 1677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 734 735 1642 2887 3101 3403 3784 734
Size: 1 907 1245 214 302 381 2787
Fragments arranged by size:
2787 1245 907 381 302 214 1
BsrBI CCG'CTC
Cuts at: 50 194 204 4129 4183 5667 5832 50
Size: 144 10 3925 54 1484 165 55
Fragments arranged by size:
3925 1484 165 144 55 54 10
BsrDI GCAATG_nn'
Cuts at: 1704 2342 2623 3749 1704
Size: 638 281 1126 3792
Fragments arranged by size:
3792 1126 638 281
BsrFI r'CCGG_y
Cuts at: 135 1004 2459 3835 4016 135
Size: 869 1455 1376 181 1956
Fragments arranged by size:
1956 1455 1376 869 181
BsrGI T'GTAC_A
Cuts at: 1077 1077
Size: 5837
BssSI C'ACGA_G
Cuts at: 283 4108 4816 283
Size: 3825 708 1304
Fragments arranged by size:
3825 1304 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1941 3214 1941
Size: 1273 4564
BstBI TT'CG_AA
Cuts at: 2526 4198 2526
Size: 1672 4165
BstXI CCAn_nnnn'nTGG
Cuts at: 1887 2298 1887
Size: 411 5426
BstYI r'GATC_y
Cuts at: 1 2862 3329 3352 3687 3933 4239 4251 4337
Size: 2861 467 23 335 246 306 12 86
Cuts at: 4337 5389 1
Size: 1052 449
Fragments arranged by size:
2861 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1320 2291 2483 2733 107
Size: 1213 971 192 250 3211
Fragments arranged by size:
3211 1213 971 250 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3787 3968 4131 3787
Size: 181 163 5493
Fragments arranged by size:
5493 181 163
BtsI GCAGTG_nn'
Cuts at: 1194 1498 1603 2313 2660 3080 1194
Size: 304 105 710 347 420 3951
Fragments arranged by size:
3951 710 420 347 304 105
DraI TTT'AAA
Cuts at: 2836 2836
Size: 5837
DrdI GACnn_nn'nnGTC
Cuts at: 3543 4887 3543
Size: 1344 4493
EaeI y'GGCC_r
Cuts at: 494 1263 2133 2480 2620 2753 3422 3596 3987
Size: 769 870 347 140 133 669 174 391
Cuts at: 3987 4014 5359 494
Size: 27 1345 972
Fragments arranged by size:
1345 972 870 769 669 391 347 174
140 133 27
EagI C'GGCC_G
Cuts at: 3422 3422
Size: 5837
EarI CTCTTCn'nnn_
Cuts at: 242 1445 2389 3860 4070 242
Size: 1203 944 1471 210 2009
Fragments arranged by size:
2009 1471 1203 944 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4045 4787 4933 5647 4045
Size: 742 146 714 4235
Fragments arranged by size:
4235 742 714 146
EcoICRI GAG'CTC
Cuts at: 1438 1755 2849 1438
Size: 317 1094 4426
Fragments arranged by size:
4426 1094 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 266 295 352 362 445 778 917 1042
Size: 126 29 57 10 83 333 139 125
Cuts at: 1042 1708 1867 1948 3662 4094 4447 5109 140
Size: 666 159 81 1714 432 353 662 868
Fragments arranged by size:
1714 868 666 662 432 353 333 159
139 126 125 83 81 57 29 10
EcoNI CCTnn'n_nnAGG
Cuts at: 1810 1810
Size: 5837
EcoO109I rG'GnC_Cy
Cuts at: 1053 1691 2370 2406 2976 1053
Size: 638 679 36 570 3914
Fragments arranged by size:
3914 679 638 570 36
EcoRI G'AATT_C
Cuts at: 2841 2841
Size: 5837
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3856 3856
Size: 5837
FspI TGC'GCA
Cuts at: 3618 3618
Size: 5837
HaeII r_GCGC'y
Cuts at: 136 331 3271 3519 4749 5520 136
Size: 195 2940 248 1230 771 453
Fragments arranged by size:
2940 1230 771 453 248 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 469 501 1054 1086 1618 1650 1781 1813 1840
Size: 32 553 32 532 32 131 32 27
Cuts at: 1840 1872 2207 2239 3679 3709 3711 3741 469
Size: 32 335 32 1440 30 2 30 2565
Fragments arranged by size:
2565 1440 553 532 335 131 32 32
32 32 32 32 30 30 27 2
HincII GTy'rAC
Cuts at: 2876 2876
Size: 5837
KasI G'GCGC_C
Cuts at: 132 3267 3515 132
Size: 3135 248 2454
Fragments arranged by size:
3135 2454 248
KpnI G_GTAC'C
Cuts at: 2315 2857 2315
Size: 542 5295
MluI A'CGCG_T
Cuts at: 5270 5270
Size: 5837
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4596 4780 4596
Size: 184 5653
MscI TGG'CCA
Cuts at: 496 1265 2135 2755 3598 496
Size: 769 870 620 843 2735
Fragments arranged by size:
2735 870 843 769 620
MslI CAynn'nnrTG
Cuts at: 502 578 667 1645 1888 2296 3826 3953 502
Size: 76 89 978 243 408 1530 127 2386
Fragments arranged by size:
2386 1530 978 408 243 127 89 76
MspA1I CmG'CkG
Cuts at: 160 648 1339 1941 2948 3262 3622 4406 4651
Size: 488 691 602 1007 314 360 784 245
Cuts at: 4651 160
Size: 1346
Fragments arranged by size:
1346 1007 784 691 602 488 360 314
245
NaeI GCC'GGC
Cuts at: 1006 4018 1006
Size: 3012 2825
NarI GG'CG_CC
Cuts at: 133 3268 3516 133
Size: 3135 248 2454
Fragments arranged by size:
3135 2454 248
NcoI C'CATG_G
Cuts at: 107 2291 107
Size: 2184 3653
NdeI CA'TA_TG
Cuts at: 783 1905 783
Size: 1122 4715
NgoMIV G'CCGG_C
Cuts at: 1004 4016 1004
Size: 3012 2825
NsiI A_TGCA'T
Cuts at: 783 783
Size: 5837
NspI r_CATG'y
Cuts at: 756 2890 3390 4993 756
Size: 2134 500 1603 1600
Fragments arranged by size:
2134 1603 1600 500
PasI CC'CwG_GG
Cuts at: 451 864 451
Size: 413 5424
PciI A'CATG_T
Cuts at: 4989 4989
Size: 5837
PflMI CCAn_nnn'nTGG
Cuts at: 415 2655 415
Size: 2240 3597
PfoI T'CCnGG_A
Cuts at: 1019 2225 2516 1019
Size: 1206 291 4340
Fragments arranged by size:
4340 1206 291
PmeI GTTT'AAAC
Cuts at: 2836 2836
Size: 5837
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1210 1242 1530 1562 2051 2083 4283 4315 1210
Size: 32 288 32 489 32 2200 32 2732
Fragments arranged by size:
2732 2200 489 288 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1053 1691 2370 1053
Size: 638 679 4520
Fragments arranged by size:
4520 679 638
PstI C_TGCA'G
Cuts at: 2884 3569 2884
Size: 685 5152
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5837
PvuII CAG'CTG
Cuts at: 1339 1941 3262 3622 1339
Size: 602 1321 360 3554
Fragments arranged by size:
3554 1321 602 360
RsrII CG'GwC_CG
Cuts at: 4032 4032
Size: 5837
SacI G_AGCT'C
Cuts at: 1440 1757 2851 1440
Size: 317 1094 4426
Fragments arranged by size:
4426 1094 317
SalI G'TCGA_C
Cuts at: 2874 2874
Size: 5837
SapI GCTCTTCn'nnn_
Cuts at: 1445 3860 4070 1445
Size: 2415 210 3212
Fragments arranged by size:
3212 2415 210
SbfI CC_TGCA'GG
Cuts at: 2884 2884
Size: 5837
ScaI AGT'ACT
Cuts at: 5354 5354
Size: 5837
SfcI C'TryA_G
Cuts at: 33 2880 3565 4533 4724 33
Size: 2847 685 968 191 1146
Fragments arranged by size:
2847 1146 968 685 191
SfoI GGC'GCC
Cuts at: 134 3269 3517 134
Size: 3135 248 2454
Fragments arranged by size:
3135 2454 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5837
SgrAI Cr'CCGG_yG
Cuts at: 135 135
Size: 5837
SmaI CCC'GGG
Cuts at: 2859 2859
Size: 5837
SmlI C'TyrA_G
Cuts at: 526 1756 2992 3907 4621 4883 526
Size: 1230 1236 915 714 262 1480
Fragments arranged by size:
1480 1236 1230 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5837
SphI G_CATG'C
Cuts at: 2890 3390 2890
Size: 500 5337
StyI C'CwwG_G
Cuts at: 107 1133 2291 2366 2971 3325 107
Size: 1026 1158 75 605 354 2619
Fragments arranged by size:
2619 1158 1026 605 354 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 856 1402 3420 3553 4012 4221 856
Size: 546 2018 133 459 209 2472
Fragments arranged by size:
2472 2018 546 459 209 133
TatI w'GTAC_w
Cuts at: 975 1077 1406 5352 975
Size: 102 329 3946 1460
Fragments arranged by size:
3946 1460 329 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 505 537 1467 1499 1954 1986 5354 5386 505
Size: 32 930 32 455 32 3368 32 956
Fragments arranged by size:
3368 956 930 455 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1793 3634 1793
Size: 1841 3996
XbaI T'CTAG_A
Cuts at: 2868 2868
Size: 5837
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1644 1887 2289 2298 3332 1644
Size: 243 402 9 1034 4149
Fragments arranged by size:
4149 1034 402 243 9
XmaI C'CCGG_G
Cuts at: 2857 2857
Size: 5837
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI ApaI AscI AvrII BaeI
BbvCI BcgI BmgBI BmtI BsiWI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI PacI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI