Restriction map for
pF1KB5486
(Circular) (Six-base) MAPSORT of: pF1KB5486.seq Check: 355 from: 1 to: 3692
pF1KB5486 3692 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 730 730
Size: 3692
Acc65I G'GTAC_C
Cuts at: 708 708
Size: 3692
AclI AA'CG_TT
Cuts at: 3532 3532
Size: 3692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 549 1517 1949 2696 2964 405
Size: 144 968 432 747 268 1133
Fragments arranged by size:
1133 968 747 432 268 144
AflIII A'CryG_T
Cuts at: 1044 2148 3125 1044
Size: 1104 977 1611
Fragments arranged by size:
1611 1104 977
AgeI A'CCGG_T
Cuts at: 2080 2080
Size: 3692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 484 484
Size: 3692
AlwNI CAG_nnn'CTG
Cuts at: 153 997 2564 153
Size: 844 1567 1281
Fragments arranged by size:
1567 1281 844
ApaLI G'TGCA_C
Cuts at: 2462 2462
Size: 3692
ApoI r'AATT_y
Cuts at: 610 696 3045 3301 610
Size: 86 2349 256 1001
Fragments arranged by size:
2349 1001 256 86
AseI AT'TA_AT
Cuts at: 20 20
Size: 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
AvaI C'yCGr_G
Cuts at: 136 712 136
Size: 576 3116
BamHI G'GATC_C
Cuts at: 717 717
Size: 3692
BanI G'GyrC_C
Cuts at: 340 622 708 1122 1370 1405 340
Size: 282 86 414 248 35 2627
Fragments arranged by size:
2627 414 282 248 86 35
BanII G_rGCy'C
Cuts at: 706 1736 706
Size: 1030 2662
BbeI G_GCGC'C
Cuts at: 1126 1374 1126
Size: 248 3444
BbsI GAAGACnn'nnnn_
Cuts at: 303 303
Size: 3692
BciVI GTATCCnnnnn_n'
Cuts at: 1590 2351 3692 1590
Size: 761 1341 1590
Fragments arranged by size:
1590 1341 761
BclI T'GATC_A
Cuts at: 1212 1212
Size: 3692
BglI GCCn_nnn'nGGC
Cuts at: 227 227
Size: 3692
BglII A'GATC_T
Cuts at: 257 560 1207 3244 257
Size: 303 647 2037 705
Fragments arranged by size:
2037 705 647 303
BlpI GC'TnA_GC
Cuts at: 373 804 373
Size: 431 3261
Bme1580I G_kGCm'C
Cuts at: 1317 1410 2466 1317
Size: 93 1056 2543
Fragments arranged by size:
2543 1056 93
BmgBI CAC'GTC
Cuts at: 309 309
Size: 3692
BmrI ACTGGGnnnn_n'
Cuts at: 904 985 1320 3464 904
Size: 81 335 2144 1132
Fragments arranged by size:
2144 1132 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 3692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 493 418
Size: 75 3617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 394 868 1747 2239 2537 196
Size: 198 474 879 492 298 1351
Fragments arranged by size:
1351 879 492 474 298 198
BsaI GGTCTCn'nnnn_
Cuts at: 3396 3396
Size: 3692
BsaAI yAC'GTr
Cuts at: 1045 1045
Size: 3692
BsaBI GATnn'nnATC
Cuts at: 6 1206 3243 6
Size: 1200 2037 455
Fragments arranged by size:
2037 1200 455
BsaHI Gr'CG_yC
Cuts at: 1123 1371 2073 3569 1123
Size: 248 702 1496 1246
Fragments arranged by size:
1496 1246 702 248
BsaWI w'CCGG_w
Cuts at: 881 1402 2080 2354 2501 2912 881
Size: 521 678 274 147 411 1661
Fragments arranged by size:
1661 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 517 547 517
Size: 30 3662
BseYI C'CCAG_C
Cuts at: 667 1117 1875 2452 2948 667
Size: 450 758 577 496 1411
Fragments arranged by size:
1411 758 577 496 450
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 502
Size: 3692
BsiEI CG_ry'CG
Cuts at: 104 1234 1280 2488 104
Size: 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 46
BsiHKAI G_wGCw'C
Cuts at: 308 706 1484 1674 2466 308
Size: 398 778 190 792 1534
Fragments arranged by size:
1534 792 778 398 190
Bsp1286I G_dGCh'C
Cuts at: 308 706 1317 1410 1484 1674 1736 2466 308
Size: 398 611 93 74 190 62 730 1534
Fragments arranged by size:
1534 730 611 398 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2912 2912
Size: 3692
BspHI T'CATG_A
Cuts at: 2868 2868
Size: 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 161 742 956 1258 1639 161
Size: 581 214 302 381 2214
Fragments arranged by size:
2214 581 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1984 2038 3522 3687 50
Size: 1934 54 1484 165 55
Fragments arranged by size:
1934 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 365 1604 365
Size: 1239 2453
BsrFI r'CCGG_y
Cuts at: 226 1690 1871 2080 226
Size: 1464 181 209 1838
Fragments arranged by size:
1838 1464 209 181
BssSI C'ACGA_G
Cuts at: 181 1963 2321 181
Size: 1782 358 1552
Fragments arranged by size:
1782 1552 358
BstAPI GCAn_nnn'nTGC
Cuts at: 153 1069 153
Size: 916 2776
BstBI TT'CG_AA
Cuts at: 2053 2053
Size: 3692
BstYI r'GATC_y
Cuts at: 1 257 560 717 1184 1207 1542 1788 2800
Size: 256 303 157 467 23 335 246 1012
Cuts at: 2800 2886 2898 3244 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 303 256 246
157 86 23 12
BtgI C'CryG_G
Cuts at: 107 239 107
Size: 132 3560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1642 1823 1986 1642
Size: 181 163 3348
Fragments arranged by size:
3348 181 163
BtsI GCAGTG_nn'
Cuts at: 664 678 935 664
Size: 14 257 3421
Fragments arranged by size:
3421 257 14
DraI TTT'AAA
Cuts at: 691 691
Size: 3692
DrdI GACnn_nn'nnGTC
Cuts at: 1398 2256 1398
Size: 858 2834
EaeI y'GGCC_r
Cuts at: 228 1277 1451 1842 1869 3214 228
Size: 1049 174 391 27 1345 706
Fragments arranged by size:
1345 1049 706 391 174 27
EagI C'GGCC_G
Cuts at: 1277 1277
Size: 3692
EarI CTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 637 1900 2210 2356 3502 637
Size: 1263 310 146 1146 827
Fragments arranged by size:
1263 1146 827 310 146
EcoICRI GAG'CTC
Cuts at: 704 704
Size: 3692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 418 493 549 1517 1949 2696 2964 405
Size: 13 75 56 968 432 747 268 1133
Fragments arranged by size:
1133 968 747 432 268 75 56 13
EcoO109I rG'GnC_Cy
Cuts at: 150 831 150
Size: 681 3011
EcoRI G'AATT_C
Cuts at: 610 696 610
Size: 86 3606
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 435 1711 435
Size: 1276 2416
FspI TGC'GCA
Cuts at: 1473 1473
Size: 3692
HaeII r_GCGC'y
Cuts at: 1126 1374 2396 3375 1126
Size: 248 1022 979 1443
Fragments arranged by size:
1443 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 517 549 1534 1564 1566 1596 517
Size: 32 985 30 2 30 2613
Fragments arranged by size:
2613 985 32 30 30 2
HincII GTy'rAC
Cuts at: 731 731
Size: 3692
KasI G'GCGC_C
Cuts at: 1122 1370 1122
Size: 248 3444
KpnI G_GTAC'C
Cuts at: 712 712
Size: 3692
MluI A'CGCG_T
Cuts at: 3125 3125
Size: 3692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2363 2547 2363
Size: 184 3508
MscI TGG'CCA
Cuts at: 1453 1453
Size: 3692
MslI CAynn'nnrTG
Cuts at: 1681 1808 1681
Size: 127 3565
MspA1I CmG'CkG
Cuts at: 803 1117 1477 2490 2735 803
Size: 314 360 1013 245 1760
Fragments arranged by size:
1760 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1873 1873
Size: 3692
NarI GG'CG_CC
Cuts at: 1123 1371 1123
Size: 248 3444
NcoI C'CATG_G
Cuts at: 107 107
Size: 3692
NgoMIV G'CCGG_C
Cuts at: 1871 1871
Size: 3692
NspI r_CATG'y
Cuts at: 429 745 1245 2152 429
Size: 316 500 907 1969
Fragments arranged by size:
1969 907 500 316
PciI A'CATG_T
Cuts at: 2148 2148
Size: 3692
PfoI T'CCnGG_A
Cuts at: 353 353
Size: 3692
PmeI GTTT'AAAC
Cuts at: 691 691
Size: 3692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 3660
PpuMI rG'GwC_Cy
Cuts at: 150 150
Size: 3692
PstI C_TGCA'G
Cuts at: 149 191 659 739 1424 149
Size: 42 468 80 685 2417
Fragments arranged by size:
2417 685 468 80 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3692
PvuII CAG'CTG
Cuts at: 1117 1477 1117
Size: 360 3332
RsrII CG'GwC_CG
Cuts at: 1887 1887
Size: 3692
SacI G_AGCT'C
Cuts at: 706 706
Size: 3692
SalI G'TCGA_C
Cuts at: 729 729
Size: 3692
SapI GCTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
SbfI CC_TGCA'GG
Cuts at: 739 739
Size: 3692
ScaI AGT'ACT
Cuts at: 3209 3209
Size: 3692
SfcI C'TryA_G
Cuts at: 33 145 187 655 735 1420 2413 2604 33
Size: 112 42 468 80 685 993 191 1121
Fragments arranged by size:
1121 993 685 468 191 112 80 42
SfoI GGC'GCC
Cuts at: 1124 1372 1124
Size: 248 3444
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
SmaI CCC'GGG
Cuts at: 714 714
Size: 3692
SmlI C'TyrA_G
Cuts at: 211 409 847 1762 2254 2516 211
Size: 198 438 915 492 262 1387
Fragments arranged by size:
1387 915 492 438 262 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3692
SphI G_CATG'C
Cuts at: 745 1245 745
Size: 500 3192
StyI C'CwwG_G
Cuts at: 107 602 826 1180 107
Size: 495 224 354 2619
Fragments arranged by size:
2619 495 354 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1275 1408 1867 2076 1275
Size: 133 459 209 2891
Fragments arranged by size:
2891 459 209 133
TatI w'GTAC_w
Cuts at: 3207 3207
Size: 3692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 3660
Tth111I GACn'n_nGTC
Cuts at: 1489 1489
Size: 3692
XbaI T'CTAG_A
Cuts at: 723 723
Size: 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 495 1187 495
Size: 692 3000
XmaI C'CCGG_G
Cuts at: 712 712
Size: 3692
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI