Restriction map for
pF1KB5485
(Circular) (Six-base) MAPSORT of: pF1KB5485.seq Check: 5636 from: 1 to: 4664
pF1KB5485 4664 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1179 1179
Size: 4664
AccI GT'mk_AC
Cuts at: 1702 1702
Size: 4664
Acc65I G'GTAC_C
Cuts at: 1680 1680
Size: 4664
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 594 1402 1637 2489 2921 3274 3936 335
Size: 259 808 235 852 432 353 662 1063
Fragments arranged by size:
1063 852 808 662 432 353 259 235
AflIII A'CryG_T
Cuts at: 2016 3816 4097 2016
Size: 1800 281 2583
Fragments arranged by size:
2583 1800 281
AleI CACnn'nnGTG
Cuts at: 1618 1618
Size: 4664
AlwNI CAG_nnn'CTG
Cuts at: 1380 1969 3407 1380
Size: 589 1438 2637
Fragments arranged by size:
2637 1438 589
ApaI G_GGCC'C
Cuts at: 137 700 137
Size: 563 4101
ApaLI G'TGCA_C
Cuts at: 3502 3502
Size: 4664
ApoI r'AATT_y
Cuts at: 663 1668 4017 4273 663
Size: 1005 2349 256 1054
Fragments arranged by size:
2349 1054 1005 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4664
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 202 356 1130 1142 1684 202
Size: 154 774 12 542 3182
Fragments arranged by size:
3182 774 542 154 12
BamHI G'GATC_C
Cuts at: 1689 1689
Size: 4664
BanI G'GyrC_C
Cuts at: 386 1680 2094 2342 2377 386
Size: 1294 414 248 35 2673
Fragments arranged by size:
2673 1294 414 248 35
BanII G_rGCy'C
Cuts at: 137 379 700 979 1542 1678 2708 137
Size: 242 321 279 563 136 1030 2093
Fragments arranged by size:
2093 1030 563 321 279 242 136
BbeI G_GCGC'C
Cuts at: 390 2098 2346 390
Size: 1708 248 2708
Fragments arranged by size:
2708 1708 248
BbsI GAAGACnn'nnnn_
Cuts at: 201 1443 201
Size: 1242 3422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1323 1357 1323
Size: 34 4630
BciVI GTATCCnnnnn_n'
Cuts at: 2562 3618 4664 2562
Size: 1056 1046 2562
Fragments arranged by size:
2562 1056 1046
BclI T'GATC_A
Cuts at: 779 2184 779
Size: 1405 3259
BglI GCCn_nnn'nGGC
Cuts at: 175 358 562 1498 175
Size: 183 204 936 3341
Fragments arranged by size:
3341 936 204 183
BglII A'GATC_T
Cuts at: 1355 2179 4216 1355
Size: 824 2037 1803
Fragments arranged by size:
2037 1803 824
BlpI GC'TnA_GC
Cuts at: 740 1776 740
Size: 1036 3628
Bme1580I G_kGCm'C
Cuts at: 137 700 1507 2289 2382 3506 137
Size: 563 807 782 93 1124 1295
Fragments arranged by size:
1295 1124 807 782 563 93
BmrI ACTGGGnnnn_n'
Cuts at: 1536 1876 1957 2292 4436 1536
Size: 340 81 335 2144 1764
Fragments arranged by size:
2144 1764 340 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 4664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 1480 311
Size: 1169 3495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1840 2719 3433 3731 928
Size: 912 879 714 298 1861
Fragments arranged by size:
1861 912 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 469 4368 469
Size: 3899 765
BsaAI yAC'GTr
Cuts at: 1430 2017 1430
Size: 587 4077
BsaBI GATnn'nnATC
Cuts at: 6 1375 2178 4215 6
Size: 1369 803 2037 455
Fragments arranged by size:
2037 1369 803 455
BsaHI Gr'CG_yC
Cuts at: 387 2095 2343 3045 4541 387
Size: 1708 248 702 1496 510
Fragments arranged by size:
1708 1496 702 510 248
BsaWI w'CCGG_w
Cuts at: 452 545 1853 2374 3052 3463 3610 3884 452
Size: 93 1308 521 678 411 147 274 1232
Fragments arranged by size:
1308 1232 678 521 411 274 147 93
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1366 1414 1366
Size: 48 4616
BseYI C'CCAG_C
Cuts at: 372 560 693 699 841 928 963 2089 2847
Size: 188 133 6 142 87 35 1126 758
Cuts at: 2847 3512 3920 372
Size: 665 408 1116
Fragments arranged by size:
1126 1116 758 665 408 188 142 133
87 35 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1108 1345 1108
Size: 237 4427
BsiEI CG_ry'CG
Cuts at: 104 2206 2252 3482 104
Size: 2102 46 1230 1286
Fragments arranged by size:
2102 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1678 2456 2646 3506 1678
Size: 778 190 860 2836
Fragments arranged by size:
2836 860 778 190
BsiWI C'GTAC_G
Cuts at: 1430 1430
Size: 4664
BsmI GAATG_Cn'
Cuts at: 883 883
Size: 4664
Bsp1286I G_dGCh'C
Cuts at: 137 379 700 979 1507 1542 1678 2289 2382
Size: 242 321 279 528 35 136 611 93
Cuts at: 2382 2456 2646 2708 3506 137
Size: 74 190 62 798 1295
Fragments arranged by size:
1295 798 611 528 321 279 242 190
136 93 74 62 35
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1179 1714 1928 2230 2611 1179
Size: 535 214 302 381 3232
Fragments arranged by size:
3232 535 381 302 214
BsrBI CCG'CTC
Cuts at: 50 447 672 1119 2956 3010 4494 4659 50
Size: 397 225 447 1837 54 1484 165 55
Fragments arranged by size:
1837 1484 447 397 225 165 55 54
BsrDI GCAATG_nn'
Cuts at: 851 1274 2576 851
Size: 423 1302 2939
Fragments arranged by size:
2939 1302 423
BsrFI r'CCGG_y
Cuts at: 413 556 1069 2662 2843 413
Size: 143 513 1593 181 2234
Fragments arranged by size:
2234 1593 513 181 143
BssHII G'CGCG_C
Cuts at: 114 114
Size: 4664
BssSI C'ACGA_G
Cuts at: 2935 3643 2935
Size: 708 3956
BstAPI GCAn_nnn'nTGC
Cuts at: 2041 2041
Size: 4664
BstBI TT'CG_AA
Cuts at: 3025 3025
Size: 4664
BstYI r'GATC_y
Cuts at: 1 1214 1355 1376 1689 2156 2179 2514 2760
Size: 1213 141 21 313 467 23 335 246
Cuts at: 2760 3066 3078 3164 4216 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1213 1052 467 449 335 313 306 246
141 86 23 21 12
BtgI C'CryG_G
Cuts at: 576 658 836 1016 1287 576
Size: 82 178 180 271 3953
Fragments arranged by size:
3953 271 180 178 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1326 2614 2795 2958 1326
Size: 1288 181 163 3032
Fragments arranged by size:
3032 1288 181 163
BtsI GCAGTG_nn'
Cuts at: 350 1907 350
Size: 1557 3107
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 885 920 1296 1331 885
Size: 35 376 35 4218
Fragments arranged by size:
4218 376 35 35
DraI TTT'AAA
Cuts at: 1663 1663
Size: 4664
DraIII CAC_nnn'GTG
Cuts at: 1511 1511
Size: 4664
DrdI GACnn_nn'nnGTC
Cuts at: 2370 3714 2370
Size: 1344 3320
EaeI y'GGCC_r
Cuts at: 431 770 906 1439 1578 2249 2423 2814 2841
Size: 339 136 533 139 671 174 391 27
Cuts at: 2841 4186 431
Size: 1345 909
Fragments arranged by size:
1345 909 671 533 391 339 174 139
136 27
EagI C'GGCC_G
Cuts at: 2249 2249
Size: 4664
EarI CTCTTCn'nnn_
Cuts at: 967 2687 2897 967
Size: 1720 210 2734
Fragments arranged by size:
2734 1720 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2872 3614 3760 4474 2872
Size: 742 146 714 3062
Fragments arranged by size:
3062 742 714 146
EcoICRI GAG'CTC
Cuts at: 1676 1676
Size: 4664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 335 594 1402 1480 1637 2489 2921 3274
Size: 24 259 808 78 157 852 432 353
Cuts at: 3274 3936 311
Size: 662 1039
Fragments arranged by size:
1039 852 808 662 432 353 259 157
78 24
EcoNI CCTnn'n_nnAGG
Cuts at: 781 1486 781
Size: 705 3959
EcoO109I rG'GnC_Cy
Cuts at: 134 1491 1803 134
Size: 1357 312 2995
Fragments arranged by size:
2995 1357 312
EcoRI G'AATT_C
Cuts at: 1668 1668
Size: 4664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4664
FspI TGC'GCA
Cuts at: 2445 2445
Size: 4664
HaeII r_GCGC'y
Cuts at: 390 2098 2346 3576 4347 390
Size: 1708 248 1230 771 707
Fragments arranged by size:
1708 1230 771 707 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 284 316 2506 2536 2538 2568 284
Size: 32 2190 30 2 30 2380
Fragments arranged by size:
2380 2190 32 30 30 2
HincII GTy'rAC
Cuts at: 813 1518 1703 813
Size: 705 185 3774
Fragments arranged by size:
3774 705 185
KasI G'GCGC_C
Cuts at: 386 2094 2342 386
Size: 1708 248 2708
Fragments arranged by size:
2708 1708 248
KpnI G_GTAC'C
Cuts at: 1684 1684
Size: 4664
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3423 3607 3423
Size: 184 4480
MscI TGG'CCA
Cuts at: 772 2425 772
Size: 1653 3011
MslI CAynn'nnrTG
Cuts at: 866 988 1618 2653 2780 866
Size: 122 630 1035 127 2750
Fragments arranged by size:
2750 1035 630 127 122
MspA1I CmG'CkG
Cuts at: 372 1447 1775 2089 2449 3233 3478 372
Size: 1075 328 314 360 784 245 1558
Fragments arranged by size:
1558 1075 784 360 328 314 245
NaeI GCC'GGC
Cuts at: 415 558 1071 2845 415
Size: 143 513 1774 2234
Fragments arranged by size:
2234 1774 513 143
NarI GG'CG_CC
Cuts at: 387 2095 2343 387
Size: 1708 248 2708
Fragments arranged by size:
2708 1708 248
NgoMIV G'CCGG_C
Cuts at: 413 556 1069 2843 413
Size: 143 513 1774 2234
Fragments arranged by size:
2234 1774 513 143
NspI r_CATG'y
Cuts at: 1717 2217 3820 1717
Size: 500 1603 2561
Fragments arranged by size:
2561 1603 500
PciI A'CATG_T
Cuts at: 3816 3816
Size: 4664
PfoI T'CCnGG_A
Cuts at: 1075 1075
Size: 4664
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 4632
PspOMI G'GGCC_C
Cuts at: 133 696 133
Size: 563 4101
PstI C_TGCA'G
Cuts at: 1607 1711 2396 1607
Size: 104 685 3875
Fragments arranged by size:
3875 685 104
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 372 1447 2089 2449 372
Size: 1075 642 360 2587
Fragments arranged by size:
2587 1075 642 360
RsrII CG'GwC_CG
Cuts at: 2859 2859
Size: 4664
SacI G_AGCT'C
Cuts at: 1678 1678
Size: 4664
SalI G'TCGA_C
Cuts at: 1701 1701
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 967 2687 2897 967
Size: 1720 210 2734
Fragments arranged by size:
2734 1720 210
SbfI CC_TGCA'GG
Cuts at: 1711 1711
Size: 4664
ScaI AGT'ACT
Cuts at: 4181 4181
Size: 4664
SexAI A'CCwGG_T
Cuts at: 512 512
Size: 4664
SfcI C'TryA_G
Cuts at: 33 1603 1707 2392 3360 3551 33
Size: 1570 104 685 968 191 1146
Fragments arranged by size:
1570 1146 968 685 191 104
SfiI GGCCn_nnn'nGGCC
Cuts at: 358 358
Size: 4664
SfoI GGC'GCC
Cuts at: 388 2096 2344 388
Size: 1708 248 2708
Fragments arranged by size:
2708 1708 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SmaI CCC'GGG
Cuts at: 358 1132 1686 358
Size: 774 554 3336
Fragments arranged by size:
3336 774 554
SmlI C'TyrA_G
Cuts at: 943 1819 2734 3448 3710 943
Size: 876 915 714 262 1897
Fragments arranged by size:
1897 915 876 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 1717 2217 1717
Size: 500 4164
SrfI GCCC'GGGC
Cuts at: 358 1132 358
Size: 774 3890
StyI C'CwwG_G
Cuts at: 382 470 704 956 1346 1798 2152 382
Size: 88 234 252 390 452 354 2894
Fragments arranged by size:
2894 452 390 354 252 234 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 187 1189 2247 2380 2839 3048 187
Size: 1002 1058 133 459 209 1803
Fragments arranged by size:
1803 1058 1002 459 209 133
TatI w'GTAC_w
Cuts at: 4179 4179
Size: 4664
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 264 296 4181 4213 264
Size: 32 3885 32 715
Fragments arranged by size:
3885 715 32 32
Tth111I GACn'n_nGTC
Cuts at: 529 2461 529
Size: 1932 2732
XbaI T'CTAG_A
Cuts at: 1695 1695
Size: 4664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 925 1143 1434 1549 2159 925
Size: 218 291 115 610 3430
Fragments arranged by size:
3430 610 291 218 115
XmaI C'CCGG_G
Cuts at: 356 1130 1684 356
Size: 774 554 3336
Fragments arranged by size:
3336 774 554
XmnI GAAnn'nnTTC
Cuts at: 265 265
Size: 4664
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BsmBI BspEI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SgrAI
SnaBI SspI StuI SwaI XhoI ZraI