Restriction map for
pF1KB5484
(Circular) (Six-base) MAPSORT of: pF1KB5484.seq Check: 7483 from: 1 to: 5063
pF1KB5484 5063 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1188 1188
Size: 5063
AccI GT'mk_AC
Cuts at: 2101 2101
Size: 5063
Acc65I G'GTAC_C
Cuts at: 2079 2079
Size: 5063
AclI AA'CG_TT
Cuts at: 4903 4903
Size: 5063
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 917 2888 3320 4067 4335 587
Size: 330 1971 432 747 268 1315
Fragments arranged by size:
1971 1315 747 432 330 268
AfeI AGC'GCT
Cuts at: 346 460 346
Size: 114 4949
AflIII A'CryG_T
Cuts at: 423 2415 3519 4496 423
Size: 1992 1104 977 990
Fragments arranged by size:
1992 1104 990 977
AgeI A'CCGG_T
Cuts at: 3451 3451
Size: 5063
AhdI GACnn_n'nnGTC
Cuts at: 1119 1119
Size: 5063
AlwNI CAG_nnn'CTG
Cuts at: 1155 1188 1221 1497 1536 2368 3935 1155
Size: 33 33 276 39 832 1567 2283
Fragments arranged by size:
2283 1567 832 276 39 33 33
ApaI G_GGCC'C
Cuts at: 116 116
Size: 5063
ApaLI G'TGCA_C
Cuts at: 3833 3833
Size: 5063
ApoI r'AATT_y
Cuts at: 538 2067 4416 4672 538
Size: 1529 2349 256 929
Fragments arranged by size:
2349 1529 929 256
AseI AT'TA_AT
Cuts at: 20 1082 20
Size: 1062 4001
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5063
AvaI C'yCGr_G
Cuts at: 150 258 678 804 969 1423 2083 150
Size: 108 420 126 165 454 660 3130
Fragments arranged by size:
3130 660 454 420 165 126 108
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 385 418 385
Size: 33 5030
BamHI G'GATC_C
Cuts at: 1419 2088 1419
Size: 669 4394
BanI G'GyrC_C
Cuts at: 290 561 1818 2079 2493 2741 2776 290
Size: 271 1257 261 414 248 35 2577
Fragments arranged by size:
2577 1257 414 271 261 248 35
BanII G_rGCy'C
Cuts at: 116 241 2077 3107 116
Size: 125 1836 1030 2072
Fragments arranged by size:
2072 1836 1030 125
BbeI G_GCGC'C
Cuts at: 294 2497 2745 294
Size: 2203 248 2612
Fragments arranged by size:
2612 2203 248
BbvCI CC'TCA_GC
Cuts at: 827 827
Size: 5063
BciVI GTATCCnnnnn_n'
Cuts at: 2961 3722 5063 2961
Size: 761 1341 2961
Fragments arranged by size:
2961 1341 761
BclI T'GATC_A
Cuts at: 1865 2583 1865
Size: 718 4345
BglI GCCn_nnn'nGGC
Cuts at: 152 274 1306 152
Size: 122 1032 3909
Fragments arranged by size:
3909 1032 122
BglII A'GATC_T
Cuts at: 2578 4615 2578
Size: 2037 3026
BlpI GC'TnA_GC
Cuts at: 2175 2175
Size: 5063
Bme1580I G_kGCm'C
Cuts at: 116 518 2688 2781 3837 116
Size: 402 2170 93 1056 1342
Fragments arranged by size:
2170 1342 1056 402 93
BmrI ACTGGGnnnn_n'
Cuts at: 1577 2275 2356 2691 4835 1577
Size: 698 81 335 2144 1805
Fragments arranged by size:
2144 1805 698 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1906 3430 1906
Size: 1524 3539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 728 1078 1210 1628 2002 2065 403
Size: 325 350 132 418 374 63 3401
Fragments arranged by size:
3401 418 374 350 325 132 63
Bpu10I CC'TnA_GC
Cuts at: 827 1529 827
Size: 702 4361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2239 3118 3610 3908 2239
Size: 879 492 298 3394
Fragments arranged by size:
3394 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4767 4767
Size: 5063
BsaAI yAC'GTr
Cuts at: 424 2416 424
Size: 1992 3071
BsaBI GATnn'nnATC
Cuts at: 6 2577 4614 6
Size: 2571 2037 455
Fragments arranged by size:
2571 2037 455
BsaHI Gr'CG_yC
Cuts at: 291 2494 2742 3444 4940 291
Size: 2203 248 702 1496 414
Fragments arranged by size:
2203 1496 702 414 248
BsaWI w'CCGG_w
Cuts at: 2252 2773 3451 3725 3872 4283 2252
Size: 521 678 274 147 411 3032
Fragments arranged by size:
3032 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 344 374 448 478 715 745 1186 1216 344
Size: 30 74 30 237 30 441 30 4191
Fragments arranged by size:
4191 441 237 74 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 856 1075 1207 1492 1549 1999 856
Size: 219 132 285 57 450 3920
Fragments arranged by size:
3920 450 285 219 132 57
BseYI C'CCAG_C
Cuts at: 326 1825 2488 3246 3823 4319 326
Size: 1499 663 758 577 496 1070
Fragments arranged by size:
1499 1070 758 663 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 655
Size: 5063
BsiEI CG_ry'CG
Cuts at: 104 251 324 2605 2651 3859 104
Size: 147 73 2281 46 1208 1308
Fragments arranged by size:
2281 1308 1208 147 73 46
BsiHKAI G_wGCw'C
Cuts at: 1028 1166 2077 2855 3045 3837 1028
Size: 138 911 778 190 792 2254
Fragments arranged by size:
2254 911 792 778 190 138
BsmI GAATG_Cn'
Cuts at: 1764 1764
Size: 5063
Bsp1286I G_dGCh'C
Cuts at: 116 241 518 1028 1166 2077 2688 2781 2855
Size: 125 277 510 138 911 611 93 74
Cuts at: 2855 3045 3107 3837 116
Size: 190 62 730 1342
Fragments arranged by size:
1342 911 730 611 510 277 190 138
125 93 74 62
BspEI T'CCGG_A
Cuts at: 4283 4283
Size: 5063
BspHI T'CATG_A
Cuts at: 4239 4239
Size: 5063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 320 1139 1188 1364 2113 2327 2629 3010 320
Size: 819 49 176 749 214 302 381 2373
Fragments arranged by size:
2373 819 749 381 302 214 176 49
BsrBI CCG'CTC
Cuts at: 50 482 1876 3355 3409 4893 5058 50
Size: 432 1394 1479 54 1484 165 55
Fragments arranged by size:
1484 1479 1394 432 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2975 2975
Size: 5063
BsrFI r'CCGG_y
Cuts at: 229 319 575 3061 3242 3451 229
Size: 90 256 2486 181 209 1841
Fragments arranged by size:
2486 1841 256 209 181 90
BssHII G'CGCG_C
Cuts at: 201 203 201
Size: 2 5061
BssSI C'ACGA_G
Cuts at: 1027 3334 3692 1027
Size: 2307 358 2398
Fragments arranged by size:
2398 2307 358
BstAPI GCAn_nnn'nTGC
Cuts at: 378 1389 2440 378
Size: 1011 1051 3001
Fragments arranged by size:
3001 1051 1011
BstBI TT'CG_AA
Cuts at: 3424 3424
Size: 5063
BstEII G'GTnAC_C
Cuts at: 778 778
Size: 5063
BstXI CCAn_nnnn'nTGG
Cuts at: 277 453 1590 277
Size: 176 1137 3750
Fragments arranged by size:
3750 1137 176
BstYI r'GATC_y
Cuts at: 1 1311 1419 2088 2555 2578 2913 3159 4171
Size: 1310 108 669 467 23 335 246 1012
Cuts at: 4171 4257 4269 4615 1
Size: 86 12 346 449
Fragments arranged by size:
1310 1012 669 467 449 346 335 246
108 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1039 1319 1039
Size: 280 4783
BtgI C'CryG_G
Cuts at: 107 452 714 1106 1415 1947 107
Size: 345 262 392 309 532 3223
Fragments arranged by size:
3223 532 392 345 309 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1233 3013 3194 3357 1233
Size: 1780 181 163 2939
Fragments arranged by size:
2939 1780 181 163
BtsI GCAGTG_nn'
Cuts at: 950 2306 950
Size: 1356 3707
DraI TTT'AAA
Cuts at: 2062 2062
Size: 5063
DrdI GACnn_nn'nnGTC
Cuts at: 1836 2769 3627 1836
Size: 933 858 3272
Fragments arranged by size:
3272 933 858
EaeI y'GGCC_r
Cuts at: 227 248 321 1409 2648 2822 3213 3240 4585
Size: 21 73 1088 1239 174 391 27 1345
Cuts at: 4585 227
Size: 705
Fragments arranged by size:
1345 1239 1088 705 391 174 73 27
21
EagI C'GGCC_G
Cuts at: 248 321 2648 248
Size: 73 2327 2663
Fragments arranged by size:
2663 2327 73
EarI CTCTTCn'nnn_
Cuts at: 833 893 899 3086 3296 833
Size: 60 6 2187 210 2600
Fragments arranged by size:
2600 2187 210 60 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1304 1339 1895 3271 3581 3727 4873 1304
Size: 35 556 1376 310 146 1146 1494
Fragments arranged by size:
1494 1376 1146 556 310 146 35
EcoICRI GAG'CTC
Cuts at: 2075 2075
Size: 5063
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 587 728 917 1078 1210 1628 2002 2065
Size: 184 141 189 161 132 418 374 63
Cuts at: 2065 2888 3320 4067 4335 403
Size: 823 432 747 268 1131
Fragments arranged by size:
1131 823 747 432 418 374 268 189
184 161 141 132 63
EcoNI CCTnn'n_nnAGG
Cuts at: 1528 1528
Size: 5063
EcoO109I rG'GnC_Cy
Cuts at: 112 255 309 366 552 660 749 1152 1579
Size: 143 54 57 186 108 89 403 427
Cuts at: 1579 2202 112
Size: 623 2973
Fragments arranged by size:
2973 623 427 403 186 143 108 89
57 54
EcoRI G'AATT_C
Cuts at: 2067 2067
Size: 5063
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3082 3082
Size: 5063
FspI TGC'GCA
Cuts at: 316 1918 2844 316
Size: 1602 926 2535
Fragments arranged by size:
2535 1602 926
HaeII r_GCGC'y
Cuts at: 294 348 462 1221 1272 1353 2497 2745 3767
Size: 54 114 759 51 81 1144 248 1022
Cuts at: 3767 4746 294
Size: 979 611
Fragments arranged by size:
1144 1022 979 759 611 248 114 81
54 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 344 376 832 864 1105 1137 1186 1218 1473
Size: 32 456 32 241 32 49 32 255
Cuts at: 1473 1505 1690 1722 1837 1869 2905 2935 2937
Size: 32 185 32 115 32 1036 30 2
Cuts at: 2937 2967 344
Size: 30 2440
Fragments arranged by size:
2440 1036 456 255 241 185 115 49
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2102 2102
Size: 5063
KasI G'GCGC_C
Cuts at: 290 2493 2741 290
Size: 2203 248 2612
Fragments arranged by size:
2612 2203 248
KpnI G_GTAC'C
Cuts at: 2083 2083
Size: 5063
MluI A'CGCG_T
Cuts at: 4496 4496
Size: 5063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3734 3918 3734
Size: 184 4879
MscI TGG'CCA
Cuts at: 2824 2824
Size: 5063
MslI CAynn'nnrTG
Cuts at: 451 1666 2018 3052 3179 451
Size: 1215 352 1034 127 2335
Fragments arranged by size:
2335 1215 1034 352 127
MspA1I CmG'CkG
Cuts at: 127 165 171 326 488 524 1734 1825 2010
Size: 38 6 155 162 36 1210 91 185
Cuts at: 2010 2174 2488 2848 3861 4106 127
Size: 164 314 360 1013 245 1084
Fragments arranged by size:
1210 1084 1013 360 314 245 185 164
162 155 91 38 36 6
NaeI GCC'GGC
Cuts at: 3244 3244
Size: 5063
NarI GG'CG_CC
Cuts at: 291 2494 2742 291
Size: 2203 248 2612
Fragments arranged by size:
2612 2203 248
NcoI C'CATG_G
Cuts at: 107 452 1106 1947 107
Size: 345 654 841 3223
Fragments arranged by size:
3223 841 654 345
NgoMIV G'CCGG_C
Cuts at: 3242 3242
Size: 5063
NspI r_CATG'y
Cuts at: 687 1008 1408 2116 2616 3523 687
Size: 321 400 708 500 907 2227
Fragments arranged by size:
2227 907 708 500 400 321
PasI CC'CwG_GG
Cuts at: 657 1606 657
Size: 949 4114
PciI A'CATG_T
Cuts at: 3519 3519
Size: 5063
PflMI CCAn_nnn'nTGG
Cuts at: 1407 1953 1407
Size: 546 4517
PfoI T'CCnGG_A
Cuts at: 362 475 1550 2042 362
Size: 113 1075 492 3383
Fragments arranged by size:
3383 1075 492 113
PmeI GTTT'AAAC
Cuts at: 2062 2062
Size: 5063
PmlI CAC'GTG
Cuts at: 424 424
Size: 5063
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 4202
Size: 32 5031
PpuMI rG'GwC_Cy
Cuts at: 309 366 749 1579 309
Size: 57 383 830 3793
Fragments arranged by size:
3793 830 383 57
PspOMI G'GGCC_C
Cuts at: 112 112
Size: 5063
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2017 2049 2017
Size: 32 5031
PstI C_TGCA'G
Cuts at: 394 416 652 1184 1307 1640 2009 2110 2795
Size: 22 236 532 123 333 369 101 685
Cuts at: 2795 394
Size: 2662
Fragments arranged by size:
2662 685 532 369 333 236 123 101
22
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5063
PvuII CAG'CTG
Cuts at: 488 1825 2488 2848 488
Size: 1337 663 360 2703
Fragments arranged by size:
2703 1337 663 360
RsrII CG'GwC_CG
Cuts at: 3258 3258
Size: 5063
SacI G_AGCT'C
Cuts at: 2077 2077
Size: 5063
SalI G'TCGA_C
Cuts at: 2100 2100
Size: 5063
SanDI GG'GwC_CC
Cuts at: 366 749 1579 366
Size: 383 830 3850
Fragments arranged by size:
3850 830 383
SapI GCTCTTCn'nnn_
Cuts at: 3086 3296 3086
Size: 210 4853
SbfI CC_TGCA'GG
Cuts at: 1307 2110 1307
Size: 803 4260
ScaI AGT'ACT
Cuts at: 388 4580 388
Size: 4192 871
SexAI A'CCwGG_T
Cuts at: 506 506
Size: 5063
SfcI C'TryA_G
Cuts at: 33 390 412 648 1180 1303 1636 2005 2106
Size: 357 22 236 532 123 333 369 101
Cuts at: 2106 2791 3784 3975 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 532 369 357 333 236
191 123 101 22
SfoI GGC'GCC
Cuts at: 292 2495 2743 292
Size: 2203 248 2612
Fragments arranged by size:
2612 2203 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5063
SmaI CCC'GGG
Cuts at: 152 260 806 2085 152
Size: 108 546 1279 3130
Fragments arranged by size:
3130 1279 546 108
SmlI C'TyrA_G
Cuts at: 2218 3133 3625 3887 2218
Size: 915 492 262 3394
Fragments arranged by size:
3394 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5063
SphI G_CATG'C
Cuts at: 687 1008 2116 2616 687
Size: 321 1108 500 3134
Fragments arranged by size:
3134 1108 500 321
SrfI GCCC'GGGC
Cuts at: 152 152
Size: 5063
StuI AGG'CCT
Cuts at: 646 646
Size: 5063
StyI C'CwwG_G
Cuts at: 107 452 1106 1280 1652 1771 1947 2197 2551
Size: 345 654 174 372 119 176 250 354
Cuts at: 2551 107
Size: 2619
Fragments arranged by size:
2619 654 372 354 345 250 176 174
119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2646 2779 3238 3447 2646
Size: 133 459 209 4262
Fragments arranged by size:
4262 459 209 133
TatI w'GTAC_w
Cuts at: 386 4578 386
Size: 4192 871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 448 480 715 747 1156 1188 4580 4612 448
Size: 32 235 32 409 32 3392 32 899
Fragments arranged by size:
3392 899 409 235 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 356 2860 356
Size: 2504 2559
XbaI T'CTAG_A
Cuts at: 2094 2094
Size: 5063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 222 984 1053 1977 2558 222
Size: 762 69 924 581 2727
Fragments arranged by size:
2727 924 762 581 69
XmaI C'CCGG_G
Cuts at: 150 258 804 2083 150
Size: 108 546 1279 3130
Fragments arranged by size:
3130 1279 546 108
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI AscI AvrII BbsI
BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PspXI SacII SfiI SgrAI SnaBI SspI SwaI
XhoI XmnI ZraI