Restriction map for
pF1KB5482
(Circular) (Six-base) MAPSORT of: pF1KB5482.seq Check: 8022 from: 1 to: 3848
pF1KB5482 3848 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 886 886
Size: 3848
Acc65I G'GTAC_C
Cuts at: 864 864
Size: 3848
AclI AA'CG_TT
Cuts at: 3688 3688
Size: 3848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1673 2105 2852 3120 1673
Size: 432 747 268 2401
Fragments arranged by size:
2401 747 432 268
AflII C'TTAA_G
Cuts at: 304 304
Size: 3848
AflIII A'CryG_T
Cuts at: 1200 2304 3281 1200
Size: 1104 977 1767
Fragments arranged by size:
1767 1104 977
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AleI CACnn'nnGTG
Cuts at: 525 525
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 1153 2720 1153
Size: 1567 2281
ApaLI G'TGCA_C
Cuts at: 2618 2618
Size: 3848
ApoI r'AATT_y
Cuts at: 532 852 3201 3457 532
Size: 320 2349 256 923
Fragments arranged by size:
2349 923 320 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 248 868 248
Size: 620 3228
BamHI G'GATC_C
Cuts at: 873 873
Size: 3848
BanI G'GyrC_C
Cuts at: 379 864 1278 1526 1561 379
Size: 485 414 248 35 2666
Fragments arranged by size:
2666 485 414 248 35
BanII G_rGCy'C
Cuts at: 862 1892 862
Size: 1030 2818
BbeI G_GCGC'C
Cuts at: 1282 1530 1282
Size: 248 3600
BbsI GAAGACnn'nnnn_
Cuts at: 294 294
Size: 3848
BbvCI CC'TCA_GC
Cuts at: 299 299
Size: 3848
BciVI GTATCCnnnnn_n'
Cuts at: 487 1746 2507 3848 487
Size: 1259 761 1341 487
Fragments arranged by size:
1341 1259 761 487
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BglI GCCn_nnn'nGGC
Cuts at: 112 338 112
Size: 226 3622
BglII A'GATC_T
Cuts at: 1363 3400 1363
Size: 2037 1811
BlpI GC'TnA_GC
Cuts at: 960 960
Size: 3848
Bme1580I G_kGCm'C
Cuts at: 1473 1566 2622 1473
Size: 93 1056 2699
Fragments arranged by size:
2699 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1060 1141 1476 3620 1060
Size: 81 335 2144 1288
Fragments arranged by size:
2144 1288 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 3848
Bpu10I CC'TnA_GC
Cuts at: 299 299
Size: 3848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1903 2395 2693 1024
Size: 879 492 298 2179
Fragments arranged by size:
2179 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3552 3552
Size: 3848
BsaAI yAC'GTr
Cuts at: 1201 1201
Size: 3848
BsaBI GATnn'nnATC
Cuts at: 6 1362 3399 6
Size: 1356 2037 455
Fragments arranged by size:
2037 1356 455
BsaHI Gr'CG_yC
Cuts at: 1279 1527 2229 3725 1279
Size: 248 702 1496 1402
Fragments arranged by size:
1496 1402 702 248
BsaWI w'CCGG_w
Cuts at: 1037 1558 2236 2510 2657 3068 1037
Size: 521 678 274 147 411 1817
Fragments arranged by size:
1817 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 199 1273 2031 2608 3104 199
Size: 1074 758 577 496 943
Fragments arranged by size:
1074 943 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 718
Size: 3848
BsiEI CG_ry'CG
Cuts at: 104 1390 1436 2644 104
Size: 1286 46 1208 1308
Fragments arranged by size:
1308 1286 1208 46
BsiHKAI G_wGCw'C
Cuts at: 862 1640 1830 2622 862
Size: 778 190 792 2088
Fragments arranged by size:
2088 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 862 1473 1566 1640 1830 1892 2622 862
Size: 611 93 74 190 62 730 2088
Fragments arranged by size:
2088 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3068 3068
Size: 3848
BspHI T'CATG_A
Cuts at: 644 3024 644
Size: 2380 1468
BspMI ACCTGCnnnn'nnnn_
Cuts at: 898 1112 1414 1795 898
Size: 214 302 381 2951
Fragments arranged by size:
2951 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2140 2194 3678 3843 50
Size: 2090 54 1484 165 55
Fragments arranged by size:
2090 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 485 1760 485
Size: 1275 2573
BsrFI r'CCGG_y
Cuts at: 1846 2027 2236 1846
Size: 181 209 3458
Fragments arranged by size:
3458 209 181
BsrGI T'GTAC_A
Cuts at: 500 716 500
Size: 216 3632
BssSI C'ACGA_G
Cuts at: 2119 2477 2119
Size: 358 3490
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1225
Size: 3848
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstYI r'GATC_y
Cuts at: 1 873 1340 1363 1698 1944 2956 3042 3054
Size: 872 467 23 335 246 1012 86 12
Cuts at: 3054 3400 1
Size: 346 449
Fragments arranged by size:
1012 872 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 730 1091 730
Size: 361 3487
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2412 1554
Size: 858 2990
EaeI y'GGCC_r
Cuts at: 710 1433 1607 1998 2025 3370 710
Size: 723 174 391 27 1345 1188
Fragments arranged by size:
1345 1188 723 391 174 27
EagI C'GGCC_G
Cuts at: 1433 1433
Size: 3848
EarI CTCTTCn'nnn_
Cuts at: 139 265 268 1871 2081 139
Size: 126 3 1603 210 1906
Fragments arranged by size:
1906 1603 210 126 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 376 2056 2366 2512 3658 376
Size: 1680 310 146 1146 566
Fragments arranged by size:
1680 1146 566 310 146
EcoICRI GAG'CTC
Cuts at: 860 860
Size: 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1673 2105 2852 3120 1673
Size: 432 747 268 2401
Fragments arranged by size:
2401 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 770 987 770
Size: 217 3631
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 489 1867 489
Size: 1378 2470
FspI TGC'GCA
Cuts at: 1629 1629
Size: 3848
HaeII r_GCGC'y
Cuts at: 1282 1530 2552 3531 1282
Size: 248 1022 979 1599
Fragments arranged by size:
1599 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 573 605 1690 1720 1722 1752 573
Size: 32 1085 30 2 30 2669
Fragments arranged by size:
2669 1085 32 30 30 2
HincII GTy'rAC
Cuts at: 887 887
Size: 3848
KasI G'GCGC_C
Cuts at: 1278 1526 1278
Size: 248 3600
KpnI G_GTAC'C
Cuts at: 868 868
Size: 3848
MluI A'CGCG_T
Cuts at: 3281 3281
Size: 3848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 2519 2703 197
Size: 2322 184 1342
Fragments arranged by size:
2322 1342 184
MscI TGG'CCA
Cuts at: 712 1609 712
Size: 897 2951
MslI CAynn'nnrTG
Cuts at: 424 525 1837 1964 424
Size: 101 1312 127 2308
Fragments arranged by size:
2308 1312 127 101
MspA1I CmG'CkG
Cuts at: 218 354 959 1273 1633 2646 2891 218
Size: 136 605 314 360 1013 245 1175
Fragments arranged by size:
1175 1013 605 360 314 245 136
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 1279 1527 1279
Size: 248 3600
NcoI C'CATG_G
Cuts at: 107 107
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NspI r_CATG'y
Cuts at: 901 1401 2308 901
Size: 500 907 2441
Fragments arranged by size:
2441 907 500
PciI A'CATG_T
Cuts at: 2304 2304
Size: 3848
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PpuMI rG'GwC_Cy
Cuts at: 770 770
Size: 3848
PstI C_TGCA'G
Cuts at: 359 681 895 1580 359
Size: 322 214 685 2627
Fragments arranged by size:
2627 685 322 214
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 218 354 1273 1633 218
Size: 136 919 360 2433
Fragments arranged by size:
2433 919 360 136
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 862 862
Size: 3848
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SanDI GG'GwC_CC
Cuts at: 770 770
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
SbfI CC_TGCA'GG
Cuts at: 895 895
Size: 3848
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SfcI C'TryA_G
Cuts at: 33 355 677 891 1576 2569 2760 33
Size: 322 322 214 685 993 191 1121
Fragments arranged by size:
1121 993 685 322 322 214 191
SfoI GGC'GCC
Cuts at: 1280 1528 1280
Size: 248 3600
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 250 870 250
Size: 620 3228
SmlI C'TyrA_G
Cuts at: 304 1003 1918 2410 2672 304
Size: 699 915 492 262 1480
Fragments arranged by size:
1480 915 699 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
StyI C'CwwG_G
Cuts at: 107 450 982 1336 107
Size: 343 532 354 2619
Fragments arranged by size:
2619 532 354 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1431 1564 2023 2232 1431
Size: 133 459 209 3047
Fragments arranged by size:
3047 459 209 133
TatI w'GTAC_w
Cuts at: 470 500 716 3363 470
Size: 30 216 2647 955
Fragments arranged by size:
2647 955 216 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 3816
Tth111I GACn'n_nGTC
Cuts at: 1645 1645
Size: 3848
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 471 1343 471
Size: 872 2976
XmaI C'CCGG_G
Cuts at: 248 868 248
Size: 620 3228
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BpmI BsaXI
BseRI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI