Restriction map for
pF1KB5481
(Circular) (Six-base) MAPSORT of: pF1KB5481.seq Check: 83 from: 1 to: 4472
pF1KB5481 4472 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 1510 1510
Size: 4472
Acc65I G'GTAC_C
Cuts at: 1488 1488
Size: 4472
AclI AA'CG_TT
Cuts at: 4312 4312
Size: 4472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 553 958 1330 2297 2729 3476 3744 312
Size: 241 405 372 967 432 747 268 1040
Fragments arranged by size:
1040 967 747 432 405 372 268 241
AflIII A'CryG_T
Cuts at: 999 1824 2928 3905 999
Size: 825 1104 977 1566
Fragments arranged by size:
1566 1104 977 825
AgeI A'CCGG_T
Cuts at: 2860 2860
Size: 4472
AlwNI CAG_nnn'CTG
Cuts at: 1105 1147 1777 3344 1105
Size: 42 630 1567 2233
Fragments arranged by size:
2233 1567 630 42
ApaLI G'TGCA_C
Cuts at: 3242 3242
Size: 4472
ApoI r'AATT_y
Cuts at: 541 633 1434 1476 3825 4081 541
Size: 92 801 42 2349 256 932
Fragments arranged by size:
2349 932 801 256 92 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 4472
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
AvaI C'yCGr_G
Cuts at: 138 1492 138
Size: 1354 3118
BamHI G'GATC_C
Cuts at: 1497 1497
Size: 4472
BanI G'GyrC_C
Cuts at: 1488 1902 2150 2185 1488
Size: 414 248 35 3775
Fragments arranged by size:
3775 414 248 35
BanII G_rGCy'C
Cuts at: 1486 2516 1486
Size: 1030 3442
BbeI G_GCGC'C
Cuts at: 1906 2154 1906
Size: 248 4224
BbsI GAAGACnn'nnnn_
Cuts at: 1262 1262
Size: 4472
BbvCI CC'TCA_GC
Cuts at: 575 575
Size: 4472
BciVI GTATCCnnnnn_n'
Cuts at: 1243 2370 3131 4472 1243
Size: 1127 761 1341 1243
Fragments arranged by size:
1341 1243 1127 761
BclI T'GATC_A
Cuts at: 1992 1992
Size: 4472
BfrBI ATG'CAT
Cuts at: 961 961
Size: 4472
BglII A'GATC_T
Cuts at: 1987 4024 1987
Size: 2037 2435
BlpI GC'TnA_GC
Cuts at: 1584 1584
Size: 4472
Bme1580I G_kGCm'C
Cuts at: 2097 2190 3246 2097
Size: 93 1056 3323
Fragments arranged by size:
3323 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1684 1765 2100 4244 1684
Size: 81 335 2144 1912
Fragments arranged by size:
2144 1912 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 4472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1169 1169
Size: 4472
Bpu10I CC'TnA_GC
Cuts at: 575 575
Size: 4472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1178 1648 2527 3019 3317 241
Size: 937 470 879 492 298 1396
Fragments arranged by size:
1396 937 879 492 470 298
BsaI GGTCTCn'nnnn_
Cuts at: 4176 4176
Size: 4472
BsaAI yAC'GTr
Cuts at: 1825 1825
Size: 4472
BsaBI GATnn'nnATC
Cuts at: 6 1986 4023 6
Size: 1980 2037 455
Fragments arranged by size:
2037 1980 455
BsaHI Gr'CG_yC
Cuts at: 1903 2151 2853 4349 1903
Size: 248 702 1496 2026
Fragments arranged by size:
2026 1496 702 248
BsaWI w'CCGG_w
Cuts at: 653 1661 2182 2860 3134 3281 3692 653
Size: 1008 521 678 274 147 411 1433
Fragments arranged by size:
1433 1008 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 730 730
Size: 4472
BseYI C'CCAG_C
Cuts at: 991 1897 2655 3232 3728 991
Size: 906 758 577 496 1735
Fragments arranged by size:
1735 906 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 968 1018 968
Size: 50 4422
BsiEI CG_ry'CG
Cuts at: 104 2014 2060 3268 104
Size: 1910 46 1208 1308
Fragments arranged by size:
1910 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 201 1486 2264 2454 3246 201
Size: 1285 778 190 792 1427
Fragments arranged by size:
1427 1285 792 778 190
BsmI GAATG_Cn'
Cuts at: 484 663 484
Size: 179 4293
Bsp1286I G_dGCh'C
Cuts at: 201 1486 2097 2190 2264 2454 2516 3246 201
Size: 1285 611 93 74 190 62 730 1427
Fragments arranged by size:
1427 1285 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3692 3692
Size: 4472
BspHI T'CATG_A
Cuts at: 3648 3648
Size: 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1522 1736 2038 2419 1522
Size: 214 302 381 3575
Fragments arranged by size:
3575 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2764 2818 4302 4467 50
Size: 2714 54 1484 165 55
Fragments arranged by size:
2714 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2384 2384
Size: 4472
BsrFI r'CCGG_y
Cuts at: 127 2470 2651 2860 127
Size: 2343 181 209 1739
Fragments arranged by size:
2343 1739 209 181
BssSI C'ACGA_G
Cuts at: 2743 3101 2743
Size: 358 4114
BstAPI GCAn_nnn'nTGC
Cuts at: 931 1087 1849 931
Size: 156 762 3554
Fragments arranged by size:
3554 762 156
BstBI TT'CG_AA
Cuts at: 2833 2833
Size: 4472
BstYI r'GATC_y
Cuts at: 1 1497 1964 1987 2322 2568 3580 3666 3678
Size: 1496 467 23 335 246 1012 86 12
Cuts at: 3678 4024 1
Size: 346 449
Fragments arranged by size:
1496 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 212 212
Size: 4472
BtgI C'CryG_G
Cuts at: 408 867 408
Size: 459 4013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2422 2603 2766 2422
Size: 181 163 4128
Fragments arranged by size:
4128 181 163
BtsI GCAGTG_nn'
Cuts at: 1715 1715
Size: 4472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 849 884 849
Size: 35 4437
DraI TTT'AAA
Cuts at: 1471 1471
Size: 4472
DrdI GACnn_nn'nnGTC
Cuts at: 904 2178 3036 904
Size: 1274 858 2340
Fragments arranged by size:
2340 1274 858
EaeI y'GGCC_r
Cuts at: 1407 2057 2231 2622 2649 3994 1407
Size: 650 174 391 27 1345 1885
Fragments arranged by size:
1885 1345 650 391 174 27
EagI C'GGCC_G
Cuts at: 2057 2057
Size: 4472
EarI CTCTTCn'nnn_
Cuts at: 2495 2705 2495
Size: 210 4262
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2680 2990 3136 4282 2680
Size: 310 146 1146 2870
Fragments arranged by size:
2870 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1484 1484
Size: 4472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 553 958 1169 1330 2297 2729 3476 3744
Size: 241 405 211 161 967 432 747 268
Cuts at: 3744 312
Size: 1040
Fragments arranged by size:
1040 967 747 432 405 268 241 211
161
EcoO109I rG'GnC_Cy
Cuts at: 216 1298 1611 216
Size: 1082 313 3077
Fragments arranged by size:
3077 1082 313
EcoRI G'AATT_C
Cuts at: 1476 1476
Size: 4472
EcoRV GAT'ATC
Cuts at: 1377 1377
Size: 4472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 779 1219 2491 779
Size: 440 1272 2760
Fragments arranged by size:
2760 1272 440
FspI TGC'GCA
Cuts at: 2253 2253
Size: 4472
HaeII r_GCGC'y
Cuts at: 1906 2154 3176 4155 1906
Size: 248 1022 979 2223
Fragments arranged by size:
2223 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 863 895 1142 1174 2314 2344 2346 2376 863
Size: 32 247 32 1140 30 2 30 2959
Fragments arranged by size:
2959 1140 247 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1511 1511
Size: 4472
KasI G'GCGC_C
Cuts at: 1902 2150 1902
Size: 248 4224
KpnI G_GTAC'C
Cuts at: 1492 1492
Size: 4472
MluI A'CGCG_T
Cuts at: 3905 3905
Size: 4472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1232 3143 3327 1232
Size: 1911 184 2377
Fragments arranged by size:
2377 1911 184
MscI TGG'CCA
Cuts at: 1409 2233 1409
Size: 824 3648
MslI CAynn'nnrTG
Cuts at: 2461 2588 2461
Size: 127 4345
MspA1I CmG'CkG
Cuts at: 184 1144 1583 1897 2257 3270 3515 184
Size: 960 439 314 360 1013 245 1141
Fragments arranged by size:
1141 1013 960 439 360 314 245
NaeI GCC'GGC
Cuts at: 129 2653 129
Size: 2524 1948
NarI GG'CG_CC
Cuts at: 1903 2151 1903
Size: 248 4224
NgoMIV G'CCGG_C
Cuts at: 127 2651 127
Size: 2524 1948
NsiI A_TGCA'T
Cuts at: 963 963
Size: 4472
NspI r_CATG'y
Cuts at: 643 982 1003 1329 1525 2025 2932 643
Size: 339 21 326 196 500 907 2183
Fragments arranged by size:
2183 907 500 339 326 196 21
PciI A'CATG_T
Cuts at: 999 2928 999
Size: 1929 2543
PfoI T'CCnGG_A
Cuts at: 1283 1283
Size: 4472
PmeI GTTT'AAAC
Cuts at: 1471 1471
Size: 4472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 803 835 3611 3643 803
Size: 32 2776 32 1632
Fragments arranged by size:
2776 1632 32 32
PpuMI rG'GwC_Cy
Cuts at: 216 1298 216
Size: 1082 3390
PshAI GACnn'nnGTC
Cuts at: 622 622
Size: 4472
PstI C_TGCA'G
Cuts at: 195 1519 2204 195
Size: 1324 685 2463
Fragments arranged by size:
2463 1324 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4472
PvuII CAG'CTG
Cuts at: 184 1144 1897 2257 184
Size: 960 753 360 2399
Fragments arranged by size:
2399 960 753 360
RsrII CG'GwC_CG
Cuts at: 2667 2667
Size: 4472
SacI G_AGCT'C
Cuts at: 1486 1486
Size: 4472
SalI G'TCGA_C
Cuts at: 1509 1509
Size: 4472
SanDI GG'GwC_CC
Cuts at: 1298 1298
Size: 4472
SapI GCTCTTCn'nnn_
Cuts at: 2495 2705 2495
Size: 210 4262
SbfI CC_TGCA'GG
Cuts at: 195 1519 195
Size: 1324 3148
ScaI AGT'ACT
Cuts at: 3989 3989
Size: 4472
SfcI C'TryA_G
Cuts at: 33 191 690 1515 2200 3193 3384 33
Size: 158 499 825 685 993 191 1121
Fragments arranged by size:
1121 993 825 685 499 191 158
SfoI GGC'GCC
Cuts at: 1904 2152 1904
Size: 248 4224
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
SmaI CCC'GGG
Cuts at: 1494 1494
Size: 4472
SmlI C'TyrA_G
Cuts at: 138 220 1193 1627 2542 3034 3296 138
Size: 82 973 434 915 492 262 1314
Fragments arranged by size:
1314 973 915 492 434 262 82
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4472
SphI G_CATG'C
Cuts at: 643 1525 2025 643
Size: 882 500 3090
Fragments arranged by size:
3090 882 500
StyI C'CwwG_G
Cuts at: 662 1049 1606 1960 662
Size: 387 557 354 3174
Fragments arranged by size:
3174 557 387 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 361 2055 2188 2647 2856 361
Size: 1694 133 459 209 1977
Fragments arranged by size:
1977 1694 459 209 133
TatI w'GTAC_w
Cuts at: 3987 3987
Size: 4472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 764 796 3989 4021 764
Size: 32 3193 32 1215
Fragments arranged by size:
3193 1215 32 32
Tth111I GACn'n_nGTC
Cuts at: 2269 2269
Size: 4472
XbaI T'CTAG_A
Cuts at: 1503 1503
Size: 4472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1967 1967
Size: 4472
XhoI C'TCGA_G
Cuts at: 138 138
Size: 4472
XmaI C'CCGG_G
Cuts at: 1492 1492
Size: 4472
XmnI GAAnn'nnTTC
Cuts at: 742 815 1203 742
Size: 73 388 4011
Fragments arranged by size:
4011 388 73
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PfoI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BglI BmgBI BmtI
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI PacI PasI PflMI
PmlI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI ZraI