Restriction map for
pF1KB5479
(Circular) (Six-base) MAPSORT of: pF1KB5479.seq Check: 1216 from: 1 to: 4571
pF1KB5479 4571 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1609 1609
Size: 4571
Acc65I G'GTAC_C
Cuts at: 492 1051 1587 492
Size: 559 536 3476
Fragments arranged by size:
3476 559 536
AclI AA'CG_TT
Cuts at: 4411 4411
Size: 4571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 649 941 1328 2396 2828 3181 3843 518
Size: 131 292 387 1068 432 353 662 1246
Fragments arranged by size:
1246 1068 662 432 387 353 292 131
AflIII A'CryG_T
Cuts at: 1923 3723 4004 1923
Size: 1800 281 2490
Fragments arranged by size:
2490 1800 281
AleI CACnn'nnGTG
Cuts at: 701 701
Size: 4571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1048 1048
Size: 4571
AlwNI CAG_nnn'CTG
Cuts at: 555 690 1876 3314 555
Size: 135 1186 1438 1812
Fragments arranged by size:
1812 1438 1186 135
ApaLI G'TGCA_C
Cuts at: 694 3409 694
Size: 2715 1856
ApoI r'AATT_y
Cuts at: 1575 3924 4180 1575
Size: 2349 256 1966
Fragments arranged by size:
2349 1966 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4571
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
AvaI C'yCGr_G
Cuts at: 1367 1591 1367
Size: 224 4347
BamHI G'GATC_C
Cuts at: 1596 1596
Size: 4571
BanI G'GyrC_C
Cuts at: 492 510 592 1051 1280 1384 1587 2001 2249
Size: 18 82 459 229 104 203 414 248
Cuts at: 2249 2284 492
Size: 35 2779
Fragments arranged by size:
2779 459 414 248 229 203 104 82
35 18
BanII G_rGCy'C
Cuts at: 724 1585 2615 724
Size: 861 1030 2680
Fragments arranged by size:
2680 1030 861
BbeI G_GCGC'C
Cuts at: 2005 2253 2005
Size: 248 4323
BbsI GAAGACnn'nnnn_
Cuts at: 929 1506 929
Size: 577 3994
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4537
BciVI GTATCCnnnnn_n'
Cuts at: 556 2469 3525 4571 556
Size: 1913 1056 1046 556
Fragments arranged by size:
1913 1056 1046 556
BclI T'GATC_A
Cuts at: 2091 2091
Size: 4571
BglI GCCn_nnn'nGGC
Cuts at: 1170 1170
Size: 4571
BglII A'GATC_T
Cuts at: 854 2086 4123 854
Size: 1232 2037 1302
Fragments arranged by size:
2037 1302 1232
BlpI GC'TnA_GC
Cuts at: 1683 1683
Size: 4571
Bme1580I G_kGCm'C
Cuts at: 698 834 852 1180 1389 1401 2196 2289 3413
Size: 136 18 328 209 12 795 93 1124
Cuts at: 3413 698
Size: 1856
Fragments arranged by size:
1856 1124 795 328 209 136 93 18
12
BmrI ACTGGGnnnn_n'
Cuts at: 264 1783 1864 2199 4343 264
Size: 1519 81 335 2144 492
Fragments arranged by size:
2144 1519 492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 419 599 2938 419
Size: 180 2339 2052
Fragments arranged by size:
2339 2052 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 231 354 397 569 202
Size: 29 123 43 172 4204
Fragments arranged by size:
4204 172 123 43 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1396 1747 2626 3340 3638 766
Size: 630 351 879 714 298 1699
Fragments arranged by size:
1699 879 714 630 351 298
BsaI GGTCTCn'nnnn_
Cuts at: 4275 4275
Size: 4571
BsaAI yAC'GTr
Cuts at: 1924 1924
Size: 4571
BsaBI GATnn'nnATC
Cuts at: 6 2085 4122 6
Size: 2079 2037 455
Fragments arranged by size:
2079 2037 455
BsaHI Gr'CG_yC
Cuts at: 2002 2250 2952 4448 2002
Size: 248 702 1496 2125
Fragments arranged by size:
2125 1496 702 248
BsaWI w'CCGG_w
Cuts at: 392 1073 1760 2281 2959 3370 3517 3791 392
Size: 681 687 521 678 411 147 274 1172
Fragments arranged by size:
1172 687 681 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1153 1183 1153
Size: 30 4541
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1171 1174 1171
Size: 3 4568
BseYI C'CCAG_C
Cuts at: 111 256 482 1996 2754 3419 3827 111
Size: 145 226 1514 758 665 408 855
Fragments arranged by size:
1514 855 758 665 408 226 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1109 1336 1505 1109
Size: 227 169 4175
Fragments arranged by size:
4175 227 169
BsiEI CG_ry'CG
Cuts at: 104 2113 2159 3389 104
Size: 2009 46 1230 1286
Fragments arranged by size:
2009 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 698 724 1585 2363 2553 3413 698
Size: 26 861 778 190 860 1856
Fragments arranged by size:
1856 861 860 778 190 26
BsmI GAATG_Cn'
Cuts at: 1083 1083
Size: 4571
Bsp1286I G_dGCh'C
Cuts at: 698 724 834 852 1180 1389 1401 1585 2196
Size: 26 110 18 328 209 12 184 611
Cuts at: 2196 2289 2363 2553 2615 3413 698
Size: 93 74 190 62 798 1856
Fragments arranged by size:
1856 798 611 328 209 190 184 110
93 74 62 26 18 12
BspHI T'CATG_A
Cuts at: 1118 3003 1118
Size: 1885 2686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 223 935 1621 1835 2137 2518 223
Size: 712 686 214 302 381 2276
Fragments arranged by size:
2276 712 686 381 302 214
BsrBI CCG'CTC
Cuts at: 50 473 2863 2917 4401 4566 50
Size: 423 2390 54 1484 165 55
Fragments arranged by size:
2390 1484 423 165 55 54
BsrDI GCAATG_nn'
Cuts at: 821 2483 821
Size: 1662 2909
BsrFI r'CCGG_y
Cuts at: 2569 2750 2569
Size: 181 4390
BssSI C'ACGA_G
Cuts at: 2842 3550 2842
Size: 708 3863
BstAPI GCAn_nnn'nTGC
Cuts at: 1948 1948
Size: 4571
BstBI TT'CG_AA
Cuts at: 2932 2932
Size: 4571
BstXI CCAn_nnnn'nTGG
Cuts at: 141 1020 1026 1428 141
Size: 879 6 402 3284
Fragments arranged by size:
3284 879 402 6
BstYI r'GATC_y
Cuts at: 1 218 332 361 854 1596 2063 2086 2421
Size: 217 114 29 493 742 467 23 335
Cuts at: 2421 2667 2973 2985 3071 4123 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 742 493 467 449 335 306 246
217 114 86 29 23 12
BtgI C'CryG_G
Cuts at: 931 1019 1427 931
Size: 88 408 4075
Fragments arranged by size:
4075 408 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 167 1221 2521 2702 2865 167
Size: 1054 1300 181 163 1873
Fragments arranged by size:
1873 1300 1054 181 163
BtsI GCAGTG_nn'
Cuts at: 678 1000 1180 1814 678
Size: 322 180 634 3435
Fragments arranged by size:
3435 634 322 180
DraI TTT'AAA
Cuts at: 1570 1570
Size: 4571
DraIII CAC_nnn'GTG
Cuts at: 272 272
Size: 4571
DrdI GACnn_nn'nnGTC
Cuts at: 2277 3621 2277
Size: 1344 3227
EaeI y'GGCC_r
Cuts at: 131 1209 2156 2330 2721 2748 4093 131
Size: 1078 947 174 391 27 1345 609
Fragments arranged by size:
1345 1078 947 609 391 174 27
EagI C'GGCC_G
Cuts at: 2156 2156
Size: 4571
EarI CTCTTCn'nnn_
Cuts at: 494 514 2594 2804 494
Size: 20 2080 210 2261
Fragments arranged by size:
2261 2080 210 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2779 3521 3667 4381 2779
Size: 742 146 714 2969
Fragments arranged by size:
2969 742 714 146
EcoICRI GAG'CTC
Cuts at: 722 1583 722
Size: 861 3710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 231 354 397 518 569 649 941 1328
Size: 29 123 43 121 51 80 292 387
Cuts at: 1328 2396 2828 3181 3843 202
Size: 1068 432 353 662 930
Fragments arranged by size:
1068 930 662 432 387 353 292 123
121 80 51 43 29
EcoNI CCTnn'n_nnAGG
Cuts at: 631 631
Size: 4571
EcoO109I rG'GnC_Cy
Cuts at: 309 504 1086 1444 1710 309
Size: 195 582 358 266 3170
Fragments arranged by size:
3170 582 358 266 195
EcoRI G'AATT_C
Cuts at: 1575 1575
Size: 4571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 4571
FspI TGC'GCA
Cuts at: 1269 2352 1269
Size: 1083 3488
HaeII r_GCGC'y
Cuts at: 282 2005 2253 3483 4254 282
Size: 1723 248 1230 771 599
Fragments arranged by size:
1723 1230 771 599 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 860 892 898 930 2413 2443 2445 2475 860
Size: 32 6 32 1483 30 2 30 2956
Fragments arranged by size:
2956 1483 32 32 30 30 6 2
HincII GTy'rAC
Cuts at: 147 1610 147
Size: 1463 3108
KasI G'GCGC_C
Cuts at: 2001 2249 2001
Size: 248 4323
KpnI G_GTAC'C
Cuts at: 496 1055 1591 496
Size: 559 536 3476
Fragments arranged by size:
3476 559 536
MluI A'CGCG_T
Cuts at: 4004 4004
Size: 4571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3330 3514 3330
Size: 184 4387
MscI TGG'CCA
Cuts at: 133 2332 133
Size: 2199 2372
MslI CAynn'nnrTG
Cuts at: 619 701 703 1024 1030 2560 2687 619
Size: 82 2 321 6 1530 127 2503
Fragments arranged by size:
2503 1530 321 127 82 6 2
MspA1I CmG'CkG
Cuts at: 260 555 1682 1996 2356 3140 3385 260
Size: 295 1127 314 360 784 245 1446
Fragments arranged by size:
1446 1127 784 360 314 295 245
NaeI GCC'GGC
Cuts at: 2752 2752
Size: 4571
NarI GG'CG_CC
Cuts at: 2002 2250 2002
Size: 248 4323
NcoI C'CATG_G
Cuts at: 1019 1427 1019
Size: 408 4163
NdeI CA'TA_TG
Cuts at: 1559 1559
Size: 4571
NgoMIV G'CCGG_C
Cuts at: 2750 2750
Size: 4571
NspI r_CATG'y
Cuts at: 129 253 618 1624 2124 3727 129
Size: 124 365 1006 500 1603 973
Fragments arranged by size:
1603 1006 973 500 365 124
PciI A'CATG_T
Cuts at: 3723 3723
Size: 4571
PflMI CCAn_nnn'nTGG
Cuts at: 180 441 555 690 180
Size: 261 114 135 4061
Fragments arranged by size:
4061 261 135 114
PmeI GTTT'AAAC
Cuts at: 1570 1570
Size: 4571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 4539
PpuMI rG'GwC_Cy
Cuts at: 309 504 1086 1444 309
Size: 195 582 358 3436
Fragments arranged by size:
3436 582 358 195
PshAI GACnn'nnGTC
Cuts at: 176 668 176
Size: 492 4079
PspXI vC'TCGA_Gb
Cuts at: 1367 1367
Size: 4571
PstI C_TGCA'G
Cuts at: 234 1365 1618 2303 234
Size: 1131 253 685 2502
Fragments arranged by size:
2502 1131 685 253
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4571
PvuII CAG'CTG
Cuts at: 555 1996 2356 555
Size: 1441 360 2770
Fragments arranged by size:
2770 1441 360
RsrII CG'GwC_CG
Cuts at: 2766 2766
Size: 4571
SacI G_AGCT'C
Cuts at: 724 1585 724
Size: 861 3710
SalI G'TCGA_C
Cuts at: 1608 1608
Size: 4571
SanDI GG'GwC_CC
Cuts at: 1086 1086
Size: 4571
SapI GCTCTTCn'nnn_
Cuts at: 514 2594 2804 514
Size: 2080 210 2281
Fragments arranged by size:
2281 2080 210
SbfI CC_TGCA'GG
Cuts at: 1618 1618
Size: 4571
ScaI AGT'ACT
Cuts at: 4088 4088
Size: 4571
SfcI C'TryA_G
Cuts at: 33 230 1361 1614 2299 3267 3458 33
Size: 197 1131 253 685 968 191 1146
Fragments arranged by size:
1146 1131 968 685 253 197 191
SfoI GGC'GCC
Cuts at: 2003 2251 2003
Size: 248 4323
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
SmaI CCC'GGG
Cuts at: 1593 1593
Size: 4571
SmlI C'TyrA_G
Cuts at: 781 1367 1411 1726 2641 3355 3617 781
Size: 586 44 315 915 714 262 1735
Fragments arranged by size:
1735 915 714 586 315 262 44
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4571
SphI G_CATG'C
Cuts at: 1624 2124 1624
Size: 500 4071
StuI AGG'CCT
Cuts at: 1378 1378
Size: 4571
StyI C'CwwG_G
Cuts at: 725 815 1013 1019 1427 1705 2059 725
Size: 90 198 6 408 278 354 3237
Fragments arranged by size:
3237 408 354 278 198 90 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 283 2154 2287 2746 2955 283
Size: 1871 133 459 209 1899
Fragments arranged by size:
1899 1871 459 209 133
TatI w'GTAC_w
Cuts at: 1150 4086 1150
Size: 2936 1635
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1153 1185 4088 4120 1153
Size: 32 2903 32 1604
Fragments arranged by size:
2903 1604 32 32
Tth111I GACn'n_nGTC
Cuts at: 2368 2368
Size: 4571
XbaI T'CTAG_A
Cuts at: 1602 1602
Size: 4571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 586 593 2066 586
Size: 7 1473 3091
Fragments arranged by size:
3091 1473 7
XhoI C'TCGA_G
Cuts at: 1367 1367
Size: 4571
XmaI C'CCGG_G
Cuts at: 1591 1591
Size: 4571
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PsiI PspOMI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI