Restriction map for
pF1KB5477
(Circular) (Six-base) MAPSORT of: pF1KB5477.seq Check: 6163 from: 1 to: 4286
pF1KB5477 4286 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1324 1324
Size: 4286
Acc65I G'GTAC_C
Cuts at: 1302 1302
Size: 4286
AclI AA'CG_TT
Cuts at: 756 4126 756
Size: 3370 916
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1223 2111 2543 2896 3558 445
Size: 778 888 432 353 662 1173
Fragments arranged by size:
1173 888 778 662 432 353
AflIII A'CryG_T
Cuts at: 1166 1638 3438 3719 1166
Size: 472 1800 281 1733
Fragments arranged by size:
1800 1733 472 281
AhdI GACnn_n'nnGTC
Cuts at: 557 557
Size: 4286
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1258 1290 1258
Size: 32 4254
AlwNI CAG_nnn'CTG
Cuts at: 423 1591 3029 423
Size: 1168 1438 1680
Fragments arranged by size:
1680 1438 1168
ApaLI G'TGCA_C
Cuts at: 3124 3124
Size: 4286
ApoI r'AATT_y
Cuts at: 1290 3639 3895 1290
Size: 2349 256 1681
Fragments arranged by size:
2349 1681 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4286
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
AvaI C'yCGr_G
Cuts at: 515 973 1306 515
Size: 458 333 3495
Fragments arranged by size:
3495 458 333
BamHI G'GATC_C
Cuts at: 375 510 575 1311 375
Size: 135 65 736 3350
Fragments arranged by size:
3350 736 135 65
BanI G'GyrC_C
Cuts at: 502 1108 1302 1716 1964 1999 502
Size: 606 194 414 248 35 2789
Fragments arranged by size:
2789 606 414 248 194 35
BanII G_rGCy'C
Cuts at: 363 1199 1300 2330 363
Size: 836 101 1030 2319
Fragments arranged by size:
2319 1030 836 101
BbeI G_GCGC'C
Cuts at: 1720 1968 1720
Size: 248 4038
BbsI GAAGACnn'nnnn_
Cuts at: 623 839 967 1066 623
Size: 216 128 99 3843
Fragments arranged by size:
3843 216 128 99
BbvCI CC'TCA_GC
Cuts at: 809 809
Size: 4286
BciVI GTATCCnnnnn_n'
Cuts at: 2184 3240 4286 2184
Size: 1056 1046 2184
Fragments arranged by size:
2184 1056 1046
BclI T'GATC_A
Cuts at: 1806 1806
Size: 4286
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4286
BglII A'GATC_T
Cuts at: 1801 3838 1801
Size: 2037 2249
BlpI GC'TnA_GC
Cuts at: 206 1398 206
Size: 1192 3094
Bme1580I G_kGCm'C
Cuts at: 507 687 1911 2004 3128 507
Size: 180 1224 93 1124 1665
Fragments arranged by size:
1665 1224 1124 180 93
BmrI ACTGGGnnnn_n'
Cuts at: 1498 1579 1914 4058 1498
Size: 81 335 2144 1726
Fragments arranged by size:
2144 1726 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 4286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 733 511
Size: 222 4064
Bpu10I CC'TnA_GC
Cuts at: 809 1008 809
Size: 199 4087
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1462 2341 3055 3353 1462
Size: 879 714 298 2395
Fragments arranged by size:
2395 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3990 3990
Size: 4286
BsaAI yAC'GTr
Cuts at: 1639 1639
Size: 4286
BsaBI GATnn'nnATC
Cuts at: 6 1800 3837 6
Size: 1794 2037 455
Fragments arranged by size:
2037 1794 455
BsaHI Gr'CG_yC
Cuts at: 1717 1965 2667 4163 1717
Size: 248 702 1496 1840
Fragments arranged by size:
1840 1496 702 248
BsaWI w'CCGG_w
Cuts at: 244 310 1475 1996 2674 3085 3232 3506 244
Size: 66 1165 521 678 411 147 274 1024
Fragments arranged by size:
1165 1024 678 521 411 274 147 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 970 1000 1258 1288 970
Size: 30 258 30 3968
Fragments arranged by size:
3968 258 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 826 829 886 991 1266 826
Size: 3 57 105 275 3846
Fragments arranged by size:
3846 275 105 57 3
BseYI C'CCAG_C
Cuts at: 1711 2469 3134 3542 1711
Size: 758 665 408 2455
Fragments arranged by size:
2455 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1155 1828 1874 3104 104
Size: 1051 673 46 1230 1286
Fragments arranged by size:
1286 1230 1051 673 46
BsiHKAI G_wGCw'C
Cuts at: 1300 2078 2268 3128 1300
Size: 778 190 860 2458
Fragments arranged by size:
2458 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 363 507 687 1199 1300 1911 2004 2078 2268
Size: 144 180 512 101 611 93 74 190
Cuts at: 2268 2330 3128 363
Size: 62 798 1521
Fragments arranged by size:
1521 798 611 512 190 180 144 101
93 74 62
BspEI T'CCGG_A
Cuts at: 310 310
Size: 4286
BspHI T'CATG_A
Cuts at: 2718 2718
Size: 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1336 1550 1852 2233 1336
Size: 214 302 381 3389
Fragments arranged by size:
3389 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1007 2578 2632 4116 4281 50
Size: 957 1571 54 1484 165 55
Fragments arranged by size:
1571 1484 957 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2198 2198
Size: 4286
BsrFI r'CCGG_y
Cuts at: 2284 2465 2284
Size: 181 4105
BssSI C'ACGA_G
Cuts at: 2557 3265 2557
Size: 708 3578
BstAPI GCAn_nnn'nTGC
Cuts at: 1085 1094 1103 1663 1085
Size: 9 9 560 3708
Fragments arranged by size:
3708 560 9 9
BstBI TT'CG_AA
Cuts at: 2647 2647
Size: 4286
BstYI r'GATC_y
Cuts at: 1 375 510 575 1311 1778 1801 2136 2382
Size: 374 135 65 736 467 23 335 246
Cuts at: 2382 2688 2700 2786 3838 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 736 467 449 374 335 306 246
135 86 65 23 12
Bsu36I CC'TnA_GG
Cuts at: 371 371
Size: 4286
BtgI C'CryG_G
Cuts at: 107 167 107
Size: 60 4226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2236 2417 2580 2236
Size: 181 163 3942
Fragments arranged by size:
3942 181 163
BtsI GCAGTG_nn'
Cuts at: 181 687 1061 1081 1529 181
Size: 506 374 20 448 2938
Fragments arranged by size:
2938 506 448 374 20
DraI TTT'AAA
Cuts at: 1285 1285
Size: 4286
DrdI GACnn_nn'nnGTC
Cuts at: 293 1992 3336 293
Size: 1699 1344 1243
Fragments arranged by size:
1699 1344 1243
EaeI y'GGCC_r
Cuts at: 1002 1871 2045 2436 2463 3808 1002
Size: 869 174 391 27 1345 1480
Fragments arranged by size:
1480 1345 869 391 174 27
EagI C'GGCC_G
Cuts at: 1871 1871
Size: 4286
EarI CTCTTCn'nnn_
Cuts at: 1141 2309 2519 1141
Size: 1168 210 2908
Fragments arranged by size:
2908 1168 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 360 2494 3236 3382 4096 360
Size: 2134 742 146 714 550
Fragments arranged by size:
2134 742 714 550 146
EcoICRI GAG'CTC
Cuts at: 1298 1298
Size: 4286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 511 733 1223 2111 2543 2896 3558 445
Size: 66 222 490 888 432 353 662 1173
Fragments arranged by size:
1173 888 662 490 432 353 222 66
EcoNI CCTnn'n_nnAGG
Cuts at: 427 427
Size: 4286
EcoO109I rG'GnC_Cy
Cuts at: 892 1425 892
Size: 533 3753
EcoRI G'AATT_C
Cuts at: 1290 1290
Size: 4286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 4286
FspI TGC'GCA
Cuts at: 478 2067 478
Size: 1589 2697
HaeII r_GCGC'y
Cuts at: 342 1720 1968 3198 3969 342
Size: 1378 248 1230 771 659
Fragments arranged by size:
1378 1230 771 659 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 543 575 761 793 865 897 970 1002 2128
Size: 32 186 32 72 32 73 32 1126
Cuts at: 2128 2158 2160 2190 543
Size: 30 2 30 2639
Fragments arranged by size:
2639 1126 186 73 72 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1325 1325
Size: 4286
HindIII A'AGCT_T
Cuts at: 1250 1250
Size: 4286
KasI G'GCGC_C
Cuts at: 1716 1964 1716
Size: 248 4038
KpnI G_GTAC'C
Cuts at: 1306 1306
Size: 4286
MfeI C'AATT_G
Cuts at: 261 261
Size: 4286
MluI A'CGCG_T
Cuts at: 3719 3719
Size: 4286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1192 3045 3229 1192
Size: 1853 184 2249
Fragments arranged by size:
2249 1853 184
MscI TGG'CCA
Cuts at: 2047 2047
Size: 4286
MslI CAynn'nnrTG
Cuts at: 274 2275 2402 274
Size: 2001 127 2158
Fragments arranged by size:
2158 2001 127
MspA1I CmG'CkG
Cuts at: 318 1070 1397 1711 2071 2855 3100 318
Size: 752 327 314 360 784 245 1504
Fragments arranged by size:
1504 784 752 360 327 314 245
NaeI GCC'GGC
Cuts at: 2467 2467
Size: 4286
NarI GG'CG_CC
Cuts at: 1717 1965 1717
Size: 248 4038
NcoI C'CATG_G
Cuts at: 107 167 107
Size: 60 4226
NgoMIV G'CCGG_C
Cuts at: 2465 2465
Size: 4286
NspI r_CATG'y
Cuts at: 1170 1339 1839 3442 1170
Size: 169 500 1603 2014
Fragments arranged by size:
2014 1603 500 169
PciI A'CATG_T
Cuts at: 1166 3438 1166
Size: 2272 2014
PfoI T'CCnGG_A
Cuts at: 297 596 710 297
Size: 299 114 3873
Fragments arranged by size:
3873 299 114
PmeI GTTT'AAAC
Cuts at: 1285 1285
Size: 4286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1258 1290 2732 2764 1258
Size: 32 1442 32 2780
Fragments arranged by size:
2780 1442 32 32
PpuMI rG'GwC_Cy
Cuts at: 892 892
Size: 4286
PstI C_TGCA'G
Cuts at: 1333 2018 1333
Size: 685 3601
PvuI CG_AT'CG
Cuts at: 104 1155 104
Size: 1051 3235
PvuII CAG'CTG
Cuts at: 318 1711 2071 318
Size: 1393 360 2533
Fragments arranged by size:
2533 1393 360
RsrII CG'GwC_CG
Cuts at: 2481 2481
Size: 4286
SacI G_AGCT'C
Cuts at: 1300 1300
Size: 4286
SalI G'TCGA_C
Cuts at: 1323 1323
Size: 4286
SapI GCTCTTCn'nnn_
Cuts at: 1141 2309 2519 1141
Size: 1168 210 2908
Fragments arranged by size:
2908 1168 210
SbfI CC_TGCA'GG
Cuts at: 1333 1333
Size: 4286
ScaI AGT'ACT
Cuts at: 154 3803 154
Size: 3649 637
SfcI C'TryA_G
Cuts at: 33 1329 2014 2982 3173 33
Size: 1296 685 968 191 1146
Fragments arranged by size:
1296 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1718 1966 1718
Size: 248 4038
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
SmaI CCC'GGG
Cuts at: 1308 1308
Size: 4286
SmlI C'TyrA_G
Cuts at: 1441 2356 3070 3332 1441
Size: 915 714 262 2395
Fragments arranged by size:
2395 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4286
SphI G_CATG'C
Cuts at: 1339 1839 1339
Size: 500 3786
StuI AGG'CCT
Cuts at: 776 776
Size: 4286
StyI C'CwwG_G
Cuts at: 107 141 167 662 1420 1774 107
Size: 34 26 495 758 354 2619
Fragments arranged by size:
2619 758 495 354 34 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 861 1869 2002 2461 2670 861
Size: 1008 133 459 209 2477
Fragments arranged by size:
2477 1008 459 209 133
TatI w'GTAC_w
Cuts at: 152 320 1169 3801 152
Size: 168 849 2632 637
Fragments arranged by size:
2632 849 637 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4254
Tth111I GACn'n_nGTC
Cuts at: 2083 2083
Size: 4286
XbaI T'CTAG_A
Cuts at: 1317 1317
Size: 4286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1781 114
Size: 1667 2619
XmaI C'CCGG_G
Cuts at: 1306 1306
Size: 4286
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI