Restriction map for
pF1KB5473
(Circular) (Six-base) MAPSORT of: pF1KB5473.seq Check: 857 from: 1 to: 3896
pF1KB5473 3896 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 163 912 163
Size: 749 3147
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 846 1721 2153 2900 3168 317
Size: 529 875 432 747 268 1045
Fragments arranged by size:
1045 875 747 529 432 268
AflIII A'CryG_T
Cuts at: 455 1248 2352 3329 455
Size: 793 1104 977 1022
Fragments arranged by size:
1104 1022 977 793
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 343 591 1201 2768 343
Size: 248 610 1567 1471
Fragments arranged by size:
1567 1471 610 248
ApaLI G'TGCA_C
Cuts at: 781 2666 781
Size: 1885 2011
ApoI r'AATT_y
Cuts at: 900 3249 3505 900
Size: 2349 256 1291
Fragments arranged by size:
2349 1291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 749 916 749
Size: 167 3729
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 163 912 1326 1574 1609 163
Size: 749 414 248 35 2450
Fragments arranged by size:
2450 749 414 248 35
BanII G_rGCy'C
Cuts at: 910 1940 910
Size: 1030 2866
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BbsI GAAGACnn'nnnn_
Cuts at: 841 841
Size: 3896
BbvCI CC'TCA_GC
Cuts at: 767 767
Size: 3896
BciVI GTATCCnnnnn_n'
Cuts at: 1794 2555 3896 1794
Size: 761 1341 1794
Fragments arranged by size:
1794 1341 761
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 785 1521 1614 2670 785
Size: 736 93 1056 2011
Fragments arranged by size:
2011 1056 736 93
BmrI ACTGGGnnnn_n'
Cuts at: 1108 1189 1524 3668 1108
Size: 81 335 2144 1336
Fragments arranged by size:
2144 1336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
Bpu10I CC'TnA_GC
Cuts at: 767 767
Size: 3896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1072 1951 2443 2741 646
Size: 426 879 492 298 1801
Fragments arranged by size:
1801 879 492 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 795 3600 795
Size: 2805 1091
BsaAI yAC'GTr
Cuts at: 786 1249 786
Size: 463 3433
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 305 1327 1575 2277 3773 305
Size: 1022 248 702 1496 428
Fragments arranged by size:
1496 1022 702 428 248
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 271
Size: 3896
BseYI C'CCAG_C
Cuts at: 478 1321 2079 2656 3152 478
Size: 843 758 577 496 1222
Fragments arranged by size:
1222 843 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 416
Size: 3896
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2692 104
Size: 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 715 785 910 1688 1878 2670 715
Size: 70 125 778 190 792 1941
Fragments arranged by size:
1941 792 778 190 125 70
BsmI GAATG_Cn'
Cuts at: 469 603 469
Size: 134 3762
Bsp1286I G_dGCh'C
Cuts at: 715 785 910 1521 1614 1688 1878 1940 2670
Size: 70 125 611 93 74 190 62 730
Cuts at: 2670 715
Size: 1941
Fragments arranged by size:
1941 730 611 190 125 93 74 70
62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 884 946 1160 1462 1843 884
Size: 62 214 302 381 2937
Fragments arranged by size:
2937 381 302 214 62
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 1808
Size: 3896
BsrFI r'CCGG_y
Cuts at: 1894 2075 2284 1894
Size: 181 209 3506
Fragments arranged by size:
3506 209 181
BsrGI T'GTAC_A
Cuts at: 554 554
Size: 3896
BssSI C'ACGA_G
Cuts at: 2167 2525 2167
Size: 358 3538
BstAPI GCAn_nnn'nTGC
Cuts at: 710 1273 710
Size: 563 3333
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstXI CCAn_nnnn'nTGG
Cuts at: 316 316
Size: 3896
BstYI r'GATC_y
Cuts at: 1 921 1388 1411 1746 1992 3004 3090 3102
Size: 920 467 23 335 246 1012 86 12
Cuts at: 3102 3448 1
Size: 346 449
Fragments arranged by size:
1012 920 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 567 567
Size: 3896
BtgI C'CryG_G
Cuts at: 107 383 107
Size: 276 3620
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 428 1139 428
Size: 711 3185
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 1481 1655 2046 2073 3418 1481
Size: 174 391 27 1345 1959
Fragments arranged by size:
1959 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 248 293 1919 2129 248
Size: 45 1626 210 2015
Fragments arranged by size:
2015 1626 210 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2104 2414 2560 3706 2104
Size: 310 146 1146 2294
Fragments arranged by size:
2294 1146 310 146
EcoICRI GAG'CTC
Cuts at: 908 908
Size: 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 846 1721 2153 2900 3168 317
Size: 529 875 432 747 268 1045
Fragments arranged by size:
1045 875 747 529 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1035 1035
Size: 3896
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
EcoRV GAT'ATC
Cuts at: 156 156
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 1330 1578 2600 3579 1330
Size: 248 1022 979 1647
Fragments arranged by size:
1647 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1738 1768 1770 1800 1738
Size: 30 2 30 3834
Fragments arranged by size:
3834 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 167 916 167
Size: 749 3147
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 1657 1657
Size: 3896
MslI CAynn'nnrTG
Cuts at: 112 314 1885 2012 112
Size: 202 1571 127 1996
Fragments arranged by size:
1996 1571 202 127
MspA1I CmG'CkG
Cuts at: 444 478 1007 1321 1681 2694 2939 444
Size: 34 529 314 360 1013 245 1401
Fragments arranged by size:
1401 1013 529 360 314 245 34
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NcoI C'CATG_G
Cuts at: 107 107
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NspI r_CATG'y
Cuts at: 459 949 1449 2356 459
Size: 490 500 907 1999
Fragments arranged by size:
1999 907 500 490
PciI A'CATG_T
Cuts at: 455 2352 455
Size: 1897 1999
PflMI CCAn_nnn'nTGG
Cuts at: 384 384
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PstI C_TGCA'G
Cuts at: 239 339 449 943 1628 239
Size: 100 110 494 685 2507
Fragments arranged by size:
2507 685 494 110 100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 444 478 1321 1681 444
Size: 34 843 360 2659
Fragments arranged by size:
2659 843 360 34
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 910 910
Size: 3896
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 235 335 445 843 939 1624 2617 2808
Size: 202 100 110 398 96 685 993 191
Cuts at: 2808 33
Size: 1121
Fragments arranged by size:
1121 993 685 398 202 191 110 100
96
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 625 749 1051 1966 2458 2720 625
Size: 124 302 915 492 262 1801
Fragments arranged by size:
1801 915 492 302 262 124
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StyI C'CwwG_G
Cuts at: 107 1030 1384 107
Size: 923 354 2619
Fragments arranged by size:
2619 923 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 111 698 1479 1612 2071 2280 111
Size: 587 781 133 459 209 1727
Fragments arranged by size:
1727 781 587 459 209 133
TatI w'GTAC_w
Cuts at: 554 3411 554
Size: 2857 1039
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 536 568 3413 3445 536
Size: 32 2845 32 987
Fragments arranged by size:
2845 987 32 32
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1391 1391
Size: 3896
XhoI C'TCGA_G
Cuts at: 749 749
Size: 3896
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI