Restriction map for
pF1KB5467
(Circular) (Six-base) MAPSORT of: pF1KB5467.seq Check: 2521 from: 1 to: 4097
pF1KB5467 4097 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1135 1135
Size: 4097
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 674 3937 674
Size: 3263 834
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 883 1922 2354 3101 3369 352
Size: 531 1039 432 747 268 1080
Fragments arranged by size:
1080 1039 747 531 432 268
AfeI AGC'GCT
Cuts at: 143 624 143
Size: 481 3616
AflIII A'CryG_T
Cuts at: 423 1449 2553 3530 423
Size: 1026 1104 977 990
Fragments arranged by size:
1104 1026 990 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AhdI GACnn_n'nnGTC
Cuts at: 454 454
Size: 4097
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 772 804 772
Size: 32 4065
AlwNI CAG_nnn'CTG
Cuts at: 193 1402 2969 193
Size: 1209 1567 1321
Fragments arranged by size:
1567 1321 1209
ApaI G_GGCC'C
Cuts at: 882 882
Size: 4097
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 615 1101 3450 3706 615
Size: 486 2349 256 1006
Fragments arranged by size:
2349 1006 486 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 137 199 1030 1117 137
Size: 62 831 87 3117
Fragments arranged by size:
3117 831 87 62
BamHI G'GATC_C
Cuts at: 863 1122 863
Size: 259 3838
BanI G'GyrC_C
Cuts at: 1025 1113 1527 1775 1810 1025
Size: 88 414 248 35 3312
Fragments arranged by size:
3312 414 248 88 35
BanII G_rGCy'C
Cuts at: 316 525 785 826 882 1111 2141 316
Size: 209 260 41 56 229 1030 2272
Fragments arranged by size:
2272 1030 260 229 209 56 41
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BbvCI CC'TCA_GC
Cuts at: 575 958 575
Size: 383 3714
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglI GCCn_nnn'nGGC
Cuts at: 112 644 997 112
Size: 532 353 3212
Fragments arranged by size:
3212 532 353
BglII A'GATC_T
Cuts at: 944 1612 3649 944
Size: 668 2037 1392
Fragments arranged by size:
2037 1392 668
BlpI GC'TnA_GC
Cuts at: 373 1209 373
Size: 836 3261
Bme1580I G_kGCm'C
Cuts at: 882 1002 1722 1815 2871 882
Size: 120 720 93 1056 2108
Fragments arranged by size:
2108 1056 720 120 93
BmrI ACTGGGnnnn_n'
Cuts at: 172 1309 1390 1725 3869 172
Size: 1137 81 335 2144 400
Fragments arranged by size:
2144 1137 400 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 152 409 493 2464 152
Size: 257 84 1971 1785
Fragments arranged by size:
1971 1785 257 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 401 488 799 214
Size: 187 87 311 3512
Fragments arranged by size:
3512 311 187 87
Bpu10I CC'TnA_GC
Cuts at: 575 772 922 958 575
Size: 197 150 36 3714
Fragments arranged by size:
3714 197 150 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 2152 2644 2942 1273
Size: 879 492 298 2428
Fragments arranged by size:
2428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3801 3801
Size: 4097
BsaAI yAC'GTr
Cuts at: 1450 1450
Size: 4097
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 1528 1776 2478 3974 1528
Size: 248 702 1496 1651
Fragments arranged by size:
1651 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 276 306 772 802 276
Size: 30 466 30 3571
Fragments arranged by size:
3571 466 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 398 398
Size: 4097
BseYI C'CCAG_C
Cuts at: 1522 2280 2857 3353 1522
Size: 758 577 496 2266
Fragments arranged by size:
2266 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 425 601 143
Size: 282 176 3639
Fragments arranged by size:
3639 282 176
BsiEI CG_ry'CG
Cuts at: 104 420 1639 1685 2893 104
Size: 316 1219 46 1208 1308
Fragments arranged by size:
1308 1219 1208 316 46
BsiHKAI G_wGCw'C
Cuts at: 525 785 1111 1889 2079 2871 525
Size: 260 326 778 190 792 1751
Fragments arranged by size:
1751 792 778 326 260 190
BsmI GAATG_Cn'
Cuts at: 279 279
Size: 4097
Bsp1286I G_dGCh'C
Cuts at: 316 525 785 826 882 1002 1111 1722 1815
Size: 209 260 41 56 120 109 611 93
Cuts at: 1815 1889 2079 2141 2871 316
Size: 74 190 62 730 1542
Fragments arranged by size:
1542 730 611 260 209 190 120 109
93 74 62 56 41
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1147 1361 1663 2044 1147
Size: 214 302 381 3200
Fragments arranged by size:
3200 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 2009
Size: 4097
BsrFI r'CCGG_y
Cuts at: 415 535 971 2095 2276 2485 415
Size: 120 436 1124 181 209 2027
Fragments arranged by size:
2027 1124 436 209 181 120
BsrGI T'GTAC_A
Cuts at: 407 407
Size: 4097
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 648 648
Size: 4097
BstYI r'GATC_y
Cuts at: 1 863 944 1122 1589 1612 1947 2193 3205
Size: 862 81 178 467 23 335 246 1012
Cuts at: 3205 3291 3303 3649 1
Size: 86 12 346 449
Fragments arranged by size:
1012 862 467 449 346 335 246 178
86 81 23 12
BtgI C'CryG_G
Cuts at: 107 317 633 1083 107
Size: 210 316 450 3121
Fragments arranged by size:
3121 450 316 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 1340 1340
Size: 4097
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 417 650 1682 1856 2247 2274 3619 417
Size: 233 1032 174 391 27 1345 895
Fragments arranged by size:
1345 1032 895 391 233 174 27
EagI C'GGCC_G
Cuts at: 417 1682 417
Size: 1265 2832
EarI CTCTTCn'nnn_
Cuts at: 328 2120 2330 328
Size: 1792 210 2095
Fragments arranged by size:
2095 1792 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 523 783 1109 523
Size: 260 326 3511
Fragments arranged by size:
3511 326 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 352 401 488 799 883 1922 2354 3101
Size: 138 49 87 311 84 1039 432 747
Cuts at: 3101 3369 214
Size: 268 942
Fragments arranged by size:
1039 942 747 432 311 268 138 87
84 49
EcoO109I rG'GnC_Cy
Cuts at: 250 264 472 879 1017 1048 1236 250
Size: 14 208 407 138 31 188 3111
Fragments arranged by size:
3111 407 208 188 138 31 14
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 134 145 626 1041 1531 1779 2801 3780 134
Size: 11 481 415 490 248 1022 979 451
Fragments arranged by size:
1022 979 490 481 451 415 248 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 440 472 915 947 1939 1969 1971 2001 440
Size: 32 443 32 992 30 2 30 2536
Fragments arranged by size:
2536 992 443 32 32 30 30 2
HincII GTy'rAC
Cuts at: 672 1136 672
Size: 464 3633
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2768 2952 2768
Size: 184 3913
MscI TGG'CCA
Cuts at: 652 1858 652
Size: 1206 2891
MslI CAynn'nnrTG
Cuts at: 1070 2086 2213 1070
Size: 1016 127 2954
Fragments arranged by size:
2954 1016 127
MspA1I CmG'CkG
Cuts at: 635 962 1208 1522 1882 2895 3140 635
Size: 327 246 314 360 1013 245 1592
Fragments arranged by size:
1592 1013 360 327 314 246 245
NaeI GCC'GGC
Cuts at: 537 973 2278 537
Size: 436 1305 2356
Fragments arranged by size:
2356 1305 436
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NcoI C'CATG_G
Cuts at: 107 1083 107
Size: 976 3121
NgoMIV G'CCGG_C
Cuts at: 535 971 2276 535
Size: 436 1305 2356
Fragments arranged by size:
2356 1305 436
NruI TCG'CGA
Cuts at: 527 527
Size: 4097
NspI r_CATG'y
Cuts at: 427 1069 1150 1650 2557 427
Size: 642 81 500 907 1967
Fragments arranged by size:
1967 907 642 500 81
PciI A'CATG_T
Cuts at: 423 2553 423
Size: 2130 1967
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 772 804 3236 3268 772
Size: 32 2432 32 1601
Fragments arranged by size:
2432 1601 32 32
PpuMI rG'GwC_Cy
Cuts at: 472 1017 472
Size: 545 3552
PspOMI G'GGCC_C
Cuts at: 878 878
Size: 4097
PspXI vC'TCGA_Gb
Cuts at: 137 137
Size: 4097
PstI C_TGCA'G
Cuts at: 120 983 1144 1829 120
Size: 863 161 685 2388
Fragments arranged by size:
2388 863 685 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 1522 1882 1522
Size: 360 3737
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 525 785 1111 525
Size: 260 326 3511
Fragments arranged by size:
3511 326 260
SacII CC_GC'GG
Cuts at: 636 636
Size: 4097
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SanDI GG'GwC_CC
Cuts at: 1017 1017
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 116 979 1140 1825 2818 3009 33
Size: 83 863 161 685 993 191 1121
Fragments arranged by size:
1121 993 863 685 191 161 83
SfiI GGCCn_nnn'nGGCC
Cuts at: 644 644
Size: 4097
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 1119 1119
Size: 4097
SmlI C'TyrA_G
Cuts at: 137 1252 2167 2659 2921 137
Size: 1115 915 492 262 1313
Fragments arranged by size:
1313 1115 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StyI C'CwwG_G
Cuts at: 107 986 1083 1231 1585 107
Size: 879 97 148 354 2619
Fragments arranged by size:
2619 879 354 148 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1680 1813 2272 2481 1680
Size: 133 459 209 3296
Fragments arranged by size:
3296 459 209 133
TatI w'GTAC_w
Cuts at: 407 3612 407
Size: 3205 892
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 227 259 276 308 3614 3646 227
Size: 32 17 32 3306 32 678
Fragments arranged by size:
3306 678 32 32 32 17
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XhoI C'TCGA_G
Cuts at: 137 137
Size: 4097
XmaI C'CCGG_G
Cuts at: 1117 1117
Size: 4097
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AscI AvrII BaeI
BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PsrI SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI