Restriction map for
pF1KB5462
(Circular) (Six-base) MAPSORT of: pF1KB5462.seq Check: 4625 from: 1 to: 4241
pF1KB5462 4241 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 677 677
Size: 4241
AccI GT'mk_AC
Cuts at: 386 1279 386
Size: 893 3348
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 2498 3245 3513 2066
Size: 432 747 268 2794
Fragments arranged by size:
2794 747 432 268
AflIII A'CryG_T
Cuts at: 830 1593 2697 3674 830
Size: 763 1104 977 1397
Fragments arranged by size:
1397 1104 977 763
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 1546 3113 1546
Size: 1567 2674
ApaI G_GGCC'C
Cuts at: 882 882
Size: 4241
ApaLI G'TGCA_C
Cuts at: 3011 3011
Size: 4241
ApoI r'AATT_y
Cuts at: 1245 3594 3850 1245
Size: 2349 256 1636
Fragments arranged by size:
2349 1636 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 456 883 1261 456
Size: 427 378 3436
Fragments arranged by size:
3436 427 378
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 1257 1671 1919 1954 1257
Size: 414 248 35 3544
Fragments arranged by size:
3544 414 248 35
BanII G_rGCy'C
Cuts at: 261 882 932 1255 2285 261
Size: 621 50 323 1030 2217
Fragments arranged by size:
2217 1030 621 323 50
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 222 256 1215 1249 222
Size: 34 959 34 3214
Fragments arranged by size:
3214 959 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BglII A'GATC_T
Cuts at: 1094 1756 3793 1094
Size: 662 2037 1542
Fragments arranged by size:
2037 1542 662
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 614 882 1866 1959 3015 614
Size: 268 984 93 1056 1840
Fragments arranged by size:
1840 1056 984 268 93
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1534 1869 4013 1453
Size: 81 335 2144 1681
Fragments arranged by size:
2144 1681 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1224 2608 1224
Size: 1384 2857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 283 829 275
Size: 8 546 3687
Fragments arranged by size:
3687 546 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2296 2788 3086 1417
Size: 879 492 298 2572
Fragments arranged by size:
2572 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 182 490 1034 3945 182
Size: 308 544 2911 478
Fragments arranged by size:
2911 544 478 308
BsaAI yAC'GTr
Cuts at: 1594 1594
Size: 4241
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 674 1672 1920 2622 4118 674
Size: 998 248 702 1496 797
Fragments arranged by size:
1496 998 797 702 248
BsaWI w'CCGG_w
Cuts at: 749 1430 1951 2629 2903 3050 3461 749
Size: 681 521 678 274 147 411 1529
Fragments arranged by size:
1529 681 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 373
Size: 30 4211
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 424 433 310
Size: 114 9 4118
Fragments arranged by size:
4118 114 9
BseYI C'CCAG_C
Cuts at: 1666 2424 3001 3497 1666
Size: 758 577 496 2410
Fragments arranged by size:
2410 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 851 844
Size: 7 4234
BsiEI CG_ry'CG
Cuts at: 104 232 669 1783 1829 3037 104
Size: 128 437 1114 46 1208 1308
Fragments arranged by size:
1308 1208 1114 437 128 46
BsiHKAI G_wGCw'C
Cuts at: 302 338 434 542 1007 1255 2033 2223 3015
Size: 36 96 108 465 248 778 190 792
Cuts at: 3015 302
Size: 1528
Fragments arranged by size:
1528 792 778 465 248 190 108 96
36
BsmBI CGTCTCn'nnnn_
Cuts at: 681 805 681
Size: 124 4117
Bsp1286I G_dGCh'C
Cuts at: 261 302 338 434 542 614 882 932 1007
Size: 41 36 96 108 72 268 50 75
Cuts at: 1007 1255 1866 1959 2033 2223 2285 3015 261
Size: 248 611 93 74 190 62 730 1487
Fragments arranged by size:
1487 730 611 268 248 190 108 96
93 75 74 72 62 50 41 36
BspEI T'CCGG_A
Cuts at: 749 3461 749
Size: 2712 1529
BspHI T'CATG_A
Cuts at: 3417 3417
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 983 1291 1505 1807 2188 983
Size: 308 214 302 381 3036
Fragments arranged by size:
3036 381 308 302 214
BsrBI CCG'CTC
Cuts at: 50 450 2533 2587 4071 4236 50
Size: 400 2083 54 1484 165 55
Fragments arranged by size:
2083 1484 400 165 55 54
BsrDI GCAATG_nn'
Cuts at: 659 2153 659
Size: 1494 2747
BsrFI r'CCGG_y
Cuts at: 510 664 2239 2420 2629 510
Size: 154 1575 181 209 2122
Fragments arranged by size:
2122 1575 209 181 154
BssSI C'ACGA_G
Cuts at: 2512 2870 2512
Size: 358 3883
BstAPI GCAn_nnn'nTGC
Cuts at: 429 537 1618 429
Size: 108 1081 3052
Fragments arranged by size:
3052 1081 108
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstYI r'GATC_y
Cuts at: 1 1094 1266 1733 1756 2091 2337 3349 3435
Size: 1093 172 467 23 335 246 1012 86
Cuts at: 3435 3447 3793 1
Size: 12 346 449
Fragments arranged by size:
1093 1012 467 449 346 335 246 172
86 23 12
BtgI C'CryG_G
Cuts at: 107 329 107
Size: 222 4019
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 111 1207 2191 2372 2535 111
Size: 1096 984 181 163 1817
Fragments arranged by size:
1817 1096 984 181 163
BtsI GCAGTG_nn'
Cuts at: 1484 1484
Size: 4241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1113 1148 1113
Size: 35 4206
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DraIII CAC_nnn'GTG
Cuts at: 342 342
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1947 2805 1947
Size: 858 3383
EaeI y'GGCC_r
Cuts at: 229 666 1826 2000 2391 2418 3763 229
Size: 437 1160 174 391 27 1345 707
Fragments arranged by size:
1345 1160 707 437 391 174 27
EagI C'GGCC_G
Cuts at: 229 666 1826 229
Size: 437 1160 2644
Fragments arranged by size:
2644 1160 437
EarI CTCTTCn'nnn_
Cuts at: 280 1193 2264 2474 280
Size: 913 1071 210 2047
Fragments arranged by size:
2047 1071 913 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 2759 2905 4051 2449
Size: 310 146 1146 2639
Fragments arranged by size:
2639 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 283 829 2066 2498 3245 3513 275
Size: 8 546 1237 432 747 268 1003
Fragments arranged by size:
1237 1003 747 546 432 268 8
EcoNI CCTnn'n_nnAGG
Cuts at: 439 439
Size: 4241
EcoO109I rG'GnC_Cy
Cuts at: 270 444 862 879 1380 270
Size: 174 418 17 501 3131
Fragments arranged by size:
3131 501 418 174 17
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
EcoRV GAT'ATC
Cuts at: 1083 1083
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 703 2022 703
Size: 1319 2922
HaeII r_GCGC'y
Cuts at: 1675 1923 2945 3924 1675
Size: 248 1022 979 1992
Fragments arranged by size:
1992 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2083 2113 2115 2145 2083
Size: 30 2 30 4179
Fragments arranged by size:
4179 30 30 2
HincII GTy'rAC
Cuts at: 717 1280 717
Size: 563 3678
HindIII A'AGCT_T
Cuts at: 1066 1066
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 714 989 2912 3096 714
Size: 275 1923 184 1859
Fragments arranged by size:
1923 1859 275 184
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 244 526 652 2230 2357 244
Size: 282 126 1578 127 2128
Fragments arranged by size:
2128 1578 282 127 126
MspA1I CmG'CkG
Cuts at: 402 1352 1666 2026 3039 3284 402
Size: 950 314 360 1013 245 1359
Fragments arranged by size:
1359 1013 950 360 314 245
NaeI GCC'GGC
Cuts at: 512 666 2422 512
Size: 154 1756 2331
Fragments arranged by size:
2331 1756 154
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NcoI C'CATG_G
Cuts at: 107 329 107
Size: 222 4019
NgoMIV G'CCGG_C
Cuts at: 510 664 2420 510
Size: 154 1756 2331
Fragments arranged by size:
2331 1756 154
NspI r_CATG'y
Cuts at: 243 1294 1794 2701 243
Size: 1051 500 907 1783
Fragments arranged by size:
1783 1051 907 500
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PflMI CCAn_nnn'nTGG
Cuts at: 141 939 141
Size: 798 3443
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 877 909 3380 3412 877
Size: 32 2471 32 1706
Fragments arranged by size:
2471 1706 32 32
PpuMI rG'GwC_Cy
Cuts at: 270 270
Size: 4241
PspOMI G'GGCC_C
Cuts at: 878 878
Size: 4241
PstI C_TGCA'G
Cuts at: 407 1288 1973 407
Size: 881 685 2675
Fragments arranged by size:
2675 881 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 402 1666 2026 402
Size: 1264 360 2617
Fragments arranged by size:
2617 1264 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 403 1284 1969 2962 3153 33
Size: 370 881 685 993 191 1121
Fragments arranged by size:
1121 993 881 685 370 191
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 1396 2311 2803 3065 1396
Size: 915 492 262 2572
Fragments arranged by size:
2572 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
StyI C'CwwG_G
Cuts at: 107 329 392 1375 1729 107
Size: 222 63 983 354 2619
Fragments arranged by size:
2619 983 354 222 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1058 1167 1824 1957 2416 2625 1058
Size: 109 657 133 459 209 2674
Fragments arranged by size:
2674 657 459 209 133 109
TatI w'GTAC_w
Cuts at: 827 1118 3756 827
Size: 291 2638 1312
Fragments arranged by size:
2638 1312 291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4209
Tth111I GACn'n_nGTC
Cuts at: 595 2038 595
Size: 1443 2798
XbaI T'CTAG_A
Cuts at: 579 1272 579
Size: 693 3548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 138 651 1736 138
Size: 513 1085 2643
Fragments arranged by size:
2643 1085 513
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
ZraI GAC'GTC
Cuts at: 675 675
Size: 4241
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI