Restriction map for
pF1KB5459
(Circular) (Six-base) MAPSORT of: pF1KB5459.seq Check: 7229 from: 1 to: 4286
pF1KB5459 4286 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 470 1324 470
Size: 854 3432
Acc65I G'GTAC_C
Cuts at: 1302 1302
Size: 4286
AclI AA'CG_TT
Cuts at: 4126 4126
Size: 4286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 2111 2543 3290 3558 1145
Size: 966 432 747 268 1873
Fragments arranged by size:
1873 966 747 432 268
AflIII A'CryG_T
Cuts at: 718 1638 2742 3719 718
Size: 920 1104 977 1285
Fragments arranged by size:
1285 1104 977 920
AgeI A'CCGG_T
Cuts at: 2674 2674
Size: 4286
AlwNI CAG_nnn'CTG
Cuts at: 1591 3158 1591
Size: 1567 2719
ApaLI G'TGCA_C
Cuts at: 3056 3056
Size: 4286
ApoI r'AATT_y
Cuts at: 1290 3639 3895 1290
Size: 2349 256 1681
Fragments arranged by size:
2349 1681 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4286
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
AvaI C'yCGr_G
Cuts at: 501 1306 501
Size: 805 3481
BamHI G'GATC_C
Cuts at: 242 283 958 1251 1311 242
Size: 41 675 293 60 3217
Fragments arranged by size:
3217 675 293 60 41
BanI G'GyrC_C
Cuts at: 579 1302 1716 1964 1999 579
Size: 723 414 248 35 2866
Fragments arranged by size:
2866 723 414 248 35
BanII G_rGCy'C
Cuts at: 1147 1300 2330 1147
Size: 153 1030 3103
Fragments arranged by size:
3103 1030 153
BbeI G_GCGC'C
Cuts at: 1720 1968 1720
Size: 248 4038
BbsI GAAGACnn'nnnn_
Cuts at: 332 350 332
Size: 18 4268
BciVI GTATCCnnnnn_n'
Cuts at: 2184 2945 4286 2184
Size: 761 1341 2184
Fragments arranged by size:
2184 1341 761
BclI T'GATC_A
Cuts at: 374 1806 374
Size: 1432 2854
BglI GCCn_nnn'nGGC
Cuts at: 754 754
Size: 4286
BglII A'GATC_T
Cuts at: 1068 1801 3838 1068
Size: 733 2037 1516
Fragments arranged by size:
2037 1516 733
BlpI GC'TnA_GC
Cuts at: 1398 1398
Size: 4286
Bme1580I G_kGCm'C
Cuts at: 1911 2004 3060 1911
Size: 93 1056 3137
Fragments arranged by size:
3137 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 486 1498 1579 1914 4058 486
Size: 1012 81 335 2144 714
Fragments arranged by size:
2144 1012 714 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 4286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 1166 1462 2341 2833 3131 500
Size: 666 296 879 492 298 1655
Fragments arranged by size:
1655 879 666 492 298 296
BsaI GGTCTCn'nnnn_
Cuts at: 375 3990 375
Size: 3615 671
BsaAI yAC'GTr
Cuts at: 1639 1639
Size: 4286
BsaBI GATnn'nnATC
Cuts at: 6 373 694 1800 3837 6
Size: 367 321 1106 2037 455
Fragments arranged by size:
2037 1106 455 367 321
BsaHI Gr'CG_yC
Cuts at: 1717 1965 2667 4163 1717
Size: 248 702 1496 1840
Fragments arranged by size:
1840 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1475 1996 2674 2948 3095 3506 1475
Size: 521 678 274 147 411 2255
Fragments arranged by size:
2255 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1088 1145 1088
Size: 57 4229
BseYI C'CCAG_C
Cuts at: 287 1711 2469 3046 3542 287
Size: 1424 758 577 496 1031
Fragments arranged by size:
1424 1031 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 1297
Size: 4286
BsiEI CG_ry'CG
Cuts at: 104 1828 1874 3082 104
Size: 1724 46 1208 1308
Fragments arranged by size:
1724 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1300 2078 2268 3060 1300
Size: 778 190 792 2526
Fragments arranged by size:
2526 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1147 1300 1911 2004 2078 2268 2330 3060 1147
Size: 153 611 93 74 190 62 730 2373
Fragments arranged by size:
2373 730 611 190 153 93 74 62
BspEI T'CCGG_A
Cuts at: 3506 3506
Size: 4286
BspHI T'CATG_A
Cuts at: 377 3462 377
Size: 3085 1201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 323 989 1269 1336 1550 1852 2233 323
Size: 666 280 67 214 302 381 2376
Fragments arranged by size:
2376 666 381 302 280 214 67
BsrBI CCG'CTC
Cuts at: 50 2578 2632 4116 4281 50
Size: 2528 54 1484 165 55
Fragments arranged by size:
2528 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2198 2198
Size: 4286
BsrFI r'CCGG_y
Cuts at: 2284 2465 2674 2284
Size: 181 209 3896
Fragments arranged by size:
3896 209 181
BssSI C'ACGA_G
Cuts at: 224 2557 2915 224
Size: 2333 358 1595
Fragments arranged by size:
2333 1595 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1663 1663
Size: 4286
BstBI TT'CG_AA
Cuts at: 2647 2647
Size: 4286
BstYI r'GATC_y
Cuts at: 1 242 283 546 958 1068 1251 1311 1778
Size: 241 41 263 412 110 183 60 467
Cuts at: 1778 1801 2136 2382 3394 3480 3492 3838 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 412 346 335 263 246
241 183 110 86 60 41 23 12
Bsu36I CC'TnA_GG
Cuts at: 425 425
Size: 4286
BtgI C'CryG_G
Cuts at: 107 107
Size: 4286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2236 2417 2580 2236
Size: 181 163 3942
Fragments arranged by size:
3942 181 163
BtsI GCAGTG_nn'
Cuts at: 180 298 1529 180
Size: 118 1231 2937
Fragments arranged by size:
2937 1231 118
DraI TTT'AAA
Cuts at: 1285 1285
Size: 4286
DrdI GACnn_nn'nnGTC
Cuts at: 1992 2850 1992
Size: 858 3428
EaeI y'GGCC_r
Cuts at: 192 780 1034 1871 2045 2436 2463 3808 192
Size: 588 254 837 174 391 27 1345 670
Fragments arranged by size:
1345 837 670 588 391 254 174 27
EagI C'GGCC_G
Cuts at: 1871 1871
Size: 4286
EarI CTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2494 2804 2950 4096 2494
Size: 310 146 1146 2684
Fragments arranged by size:
2684 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1298 1298
Size: 4286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 2111 2543 3290 3558 1145
Size: 966 432 747 268 1873
Fragments arranged by size:
1873 966 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 430 1425 430
Size: 995 3291
EcoRI G'AATT_C
Cuts at: 1290 1290
Size: 4286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 349 429 549 2305 349
Size: 80 120 1756 2330
Fragments arranged by size:
2330 1756 120 80
FspI TGC'GCA
Cuts at: 849 2067 849
Size: 1218 3068
FspAI rTGC'GCAy
Cuts at: 849 849
Size: 4286
HaeII r_GCGC'y
Cuts at: 1720 1968 2990 3969 1720
Size: 248 1022 979 2037
Fragments arranged by size:
2037 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2128 2158 2160 2190 2128
Size: 30 2 30 4224
Fragments arranged by size:
4224 30 30 2
HincII GTy'rAC
Cuts at: 1325 1325
Size: 4286
KasI G'GCGC_C
Cuts at: 1716 1964 1716
Size: 248 4038
KpnI G_GTAC'C
Cuts at: 1306 1306
Size: 4286
MluI A'CGCG_T
Cuts at: 3719 3719
Size: 4286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2957 3141 2957
Size: 184 4102
MscI TGG'CCA
Cuts at: 782 1036 2047 782
Size: 254 1011 3021
Fragments arranged by size:
3021 1011 254
MslI CAynn'nnrTG
Cuts at: 112 2275 2402 112
Size: 2163 127 1996
Fragments arranged by size:
2163 1996 127
MspA1I CmG'CkG
Cuts at: 1397 1711 2071 3084 3329 1397
Size: 314 360 1013 245 2354
Fragments arranged by size:
2354 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2467 2467
Size: 4286
NarI GG'CG_CC
Cuts at: 1717 1965 1717
Size: 248 4038
NcoI C'CATG_G
Cuts at: 107 107
Size: 4286
NgoMIV G'CCGG_C
Cuts at: 2465 2465
Size: 4286
NspI r_CATG'y
Cuts at: 621 722 848 1339 1839 2746 621
Size: 101 126 491 500 907 2161
Fragments arranged by size:
2161 907 500 491 126 101
PciI A'CATG_T
Cuts at: 718 2742 718
Size: 2024 2262
PfoI T'CCnGG_A
Cuts at: 954 954
Size: 4286
PmeI GTTT'AAAC
Cuts at: 1285 1285
Size: 4286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 391 423 1204 1236 3425 3457 391
Size: 32 781 32 2189 32 1220
Fragments arranged by size:
2189 1220 781 32 32 32
PstI C_TGCA'G
Cuts at: 1333 2018 1333
Size: 685 3601
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4286
PvuII CAG'CTG
Cuts at: 1711 2071 1711
Size: 360 3926
RsrII CG'GwC_CG
Cuts at: 2481 2481
Size: 4286
SacI G_AGCT'C
Cuts at: 1300 1300
Size: 4286
SalI G'TCGA_C
Cuts at: 1323 1323
Size: 4286
SapI GCTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
SbfI CC_TGCA'GG
Cuts at: 1333 1333
Size: 4286
ScaI AGT'ACT
Cuts at: 3803 3803
Size: 4286
SfcI C'TryA_G
Cuts at: 33 1329 2014 3007 3198 33
Size: 1296 685 993 191 1121
Fragments arranged by size:
1296 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1718 1966 1718
Size: 248 4038
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
SmaI CCC'GGG
Cuts at: 1308 1308
Size: 4286
SmlI C'TyrA_G
Cuts at: 515 1181 1441 2356 2848 3110 515
Size: 666 260 915 492 262 1691
Fragments arranged by size:
1691 915 666 492 262 260
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4286
SphI G_CATG'C
Cuts at: 1339 1839 1339
Size: 500 3786
SspI AAT'ATT
Cuts at: 928 928
Size: 4286
StuI AGG'CCT
Cuts at: 891 891
Size: 4286
StyI C'CwwG_G
Cuts at: 107 788 1420 1774 107
Size: 681 632 354 2619
Fragments arranged by size:
2619 681 632 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 452 468 1869 2002 2461 2670 452
Size: 16 1401 133 459 209 2068
Fragments arranged by size:
2068 1401 459 209 133 16
TatI w'GTAC_w
Cuts at: 401 3801 401
Size: 3400 886
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4254
Tth111I GACn'n_nGTC
Cuts at: 2083 2083
Size: 4286
XbaI T'CTAG_A
Cuts at: 1317 1317
Size: 4286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1781 1781
Size: 4286
XmaI C'CCGG_G
Cuts at: 1306 1306
Size: 4286
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI