Restriction map for
pF1KB5457
(Circular) (Six-base) MAPSORT of: pF1KB5457.seq Check: 9510 from: 1 to: 4562
pF1KB5457 4562 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 863 863
Size: 4562
AccI GT'mk_AC
Cuts at: 1600 1600
Size: 4562
Acc65I G'GTAC_C
Cuts at: 372 1578 372
Size: 1206 3356
AclI AA'CG_TT
Cuts at: 4402 4402
Size: 4562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1006 1039 1249 2387 2819 3172 3834 322
Size: 684 33 210 1138 432 353 662 1050
Fragments arranged by size:
1138 1050 684 662 432 353 210 33
AflIII A'CryG_T
Cuts at: 1914 3714 3995 1914
Size: 1800 281 2481
Fragments arranged by size:
2481 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 871 1381 871
Size: 510 4052
AlwNI CAG_nnn'CTG
Cuts at: 279 1867 3305 279
Size: 1588 1438 1536
Fragments arranged by size:
1588 1536 1438
ApaI G_GGCC'C
Cuts at: 513 513
Size: 4562
ApaLI G'TGCA_C
Cuts at: 664 3400 664
Size: 2736 1826
ApoI r'AATT_y
Cuts at: 414 467 998 1566 3915 4171 414
Size: 53 531 568 2349 256 805
Fragments arranged by size:
2349 805 568 531 256 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 4562
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4562
AvaI C'yCGr_G
Cuts at: 1582 1582
Size: 4562
AvrII C'CTAG_G
Cuts at: 1224 1224
Size: 4562
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 364 397 364
Size: 33 4529
BamHI G'GATC_C
Cuts at: 1587 1587
Size: 4562
BanI G'GyrC_C
Cuts at: 268 372 443 1528 1578 1992 2240 2275 268
Size: 104 71 1085 50 414 248 35 2555
Fragments arranged by size:
2555 1085 414 248 104 71 50 35
BanII G_rGCy'C
Cuts at: 513 1464 1576 2606 513
Size: 951 112 1030 2469
Fragments arranged by size:
2469 1030 951 112
BbeI G_GCGC'C
Cuts at: 1996 2244 1996
Size: 248 4314
BciVI GTATCCnnnnn_n'
Cuts at: 378 2460 3516 4562 378
Size: 2082 1056 1046 378
Fragments arranged by size:
2082 1056 1046 378
BclI T'GATC_A
Cuts at: 2082 2082
Size: 4562
BfrBI ATG'CAT
Cuts at: 1243 1243
Size: 4562
BglI GCCn_nnn'nGGC
Cuts at: 244 1526 244
Size: 1282 3280
BglII A'GATC_T
Cuts at: 2077 4114 2077
Size: 2037 2525
BlpI GC'TnA_GC
Cuts at: 1442 1674 1442
Size: 232 4330
Bme1580I G_kGCm'C
Cuts at: 273 513 668 895 2187 2280 3404 273
Size: 240 155 227 1292 93 1124 1431
Fragments arranged by size:
1431 1292 1124 240 227 155 93
BmgBI CAC'GTC
Cuts at: 729 729
Size: 4562
BmrI ACTGGGnnnn_n'
Cuts at: 267 633 1352 1774 1855 2190 4334 267
Size: 366 719 422 81 335 2144 495
Fragments arranged by size:
2144 719 495 422 366 335 81
BmtI G_CTAG'C
Cuts at: 1010 1010
Size: 4562
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 309 810 2929 309
Size: 501 2119 1942
Fragments arranged by size:
2119 1942 501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 1087 626
Size: 461 4101
Bpu10I CC'TnA_GC
Cuts at: 1132 1132
Size: 4562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 811 1100 1738 2617 3331 3629 439
Size: 372 289 638 879 714 298 1372
Fragments arranged by size:
1372 879 714 638 372 298 289
BsaI GGTCTCn'nnnn_
Cuts at: 297 443 4266 297
Size: 146 3823 593
Fragments arranged by size:
3823 593 146
BsaAI yAC'GTr
Cuts at: 1915 1915
Size: 4562
BsaBI GATnn'nnATC
Cuts at: 6 808 2076 4113 6
Size: 802 1268 2037 455
Fragments arranged by size:
2037 1268 802 455
BsaHI Gr'CG_yC
Cuts at: 1993 2241 2943 4439 1993
Size: 248 702 1496 2116
Fragments arranged by size:
2116 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1751 2272 2950 3361 3508 3782 1751
Size: 521 678 411 147 274 2531
Fragments arranged by size:
2531 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 944 974 944
Size: 30 4532
BseYI C'CCAG_C
Cuts at: 337 732 1987 2745 3410 3818 337
Size: 395 1255 758 665 408 1081
Fragments arranged by size:
1255 1081 758 665 408 395
BsiEI CG_ry'CG
Cuts at: 104 2104 2150 3380 104
Size: 2000 46 1230 1286
Fragments arranged by size:
2000 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 668 1576 2354 2544 3404 668
Size: 908 778 190 860 1826
Fragments arranged by size:
1826 908 860 778 190
BsmI GAATG_Cn'
Cuts at: 1358 1358
Size: 4562
Bsp1286I G_dGCh'C
Cuts at: 273 513 668 895 1464 1576 2187 2280 2354
Size: 240 155 227 569 112 611 93 74
Cuts at: 2354 2544 2606 3404 273
Size: 190 62 798 1431
Fragments arranged by size:
1431 798 611 569 240 227 190 155
112 93 74 62
BspHI T'CATG_A
Cuts at: 2994 2994
Size: 4562
BspMI ACCTGCnnnn'nnnn_
Cuts at: 863 1612 1826 2128 2509 863
Size: 749 214 302 381 2916
Fragments arranged by size:
2916 749 381 302 214
BsrBI CCG'CTC
Cuts at: 50 485 2854 2908 4392 4557 50
Size: 435 2369 54 1484 165 55
Fragments arranged by size:
2369 1484 435 165 55 54
BsrDI GCAATG_nn'
Cuts at: 584 2474 584
Size: 1890 2672
BsrFI r'CCGG_y
Cuts at: 2560 2741 2560
Size: 181 4381
BssSI C'ACGA_G
Cuts at: 2833 3541 2833
Size: 708 3854
BstAPI GCAn_nnn'nTGC
Cuts at: 1939 1939
Size: 4562
BstBI TT'CG_AA
Cuts at: 2923 2923
Size: 4562
BstXI CCAn_nnnn'nTGG
Cuts at: 264 1045 264
Size: 781 3781
BstYI r'GATC_y
Cuts at: 1 1587 2054 2077 2412 2658 2964 2976 3062
Size: 1586 467 23 335 246 306 12 86
Cuts at: 3062 4114 1
Size: 1052 449
Fragments arranged by size:
1586 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 180 1521 107
Size: 73 1341 3148
Fragments arranged by size:
3148 1341 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 108 509 1164 2512 2693 2856 108
Size: 401 655 1348 181 163 1814
Fragments arranged by size:
1814 1348 655 401 181 163
BtsI GCAGTG_nn'
Cuts at: 1379 1805 1379
Size: 426 4136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1134 1155 1169 1190 1134
Size: 21 14 21 4506
Fragments arranged by size:
4506 21 21 14
DraI TTT'AAA
Cuts at: 1561 1561
Size: 4562
DraIII CAC_nnn'GTG
Cuts at: 654 654
Size: 4562
DrdI GACnn_nn'nnGTC
Cuts at: 2268 3612 2268
Size: 1344 3218
EaeI y'GGCC_r
Cuts at: 140 236 1481 2147 2321 2712 2739 4084 140
Size: 96 1245 666 174 391 27 1345 618
Fragments arranged by size:
1345 1245 666 618 391 174 96 27
EagI C'GGCC_G
Cuts at: 2147 2147
Size: 4562
EarI CTCTTCn'nnn_
Cuts at: 2585 2795 2585
Size: 210 4352
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 330 906 2770 3512 3658 4372 330
Size: 576 1864 742 146 714 520
Fragments arranged by size:
1864 742 714 576 520 146
EcoICRI GAG'CTC
Cuts at: 1574 1574
Size: 4562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 626 1006 1039 1087 1249 2387 2819 3172
Size: 304 380 33 48 162 1138 432 353
Cuts at: 3172 3834 322
Size: 662 1050
Fragments arranged by size:
1138 1050 662 432 380 353 304 162
48 33
EcoNI CCTnn'n_nnAGG
Cuts at: 331 1435 331
Size: 1104 3458
EcoO109I rG'GnC_Cy
Cuts at: 509 1024 1701 509
Size: 515 677 3370
Fragments arranged by size:
3370 677 515
EcoRI G'AATT_C
Cuts at: 998 1566 998
Size: 568 3994
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 591 2581 591
Size: 1990 2572
FspI TGC'GCA
Cuts at: 2343 2343
Size: 4562
HaeII r_GCGC'y
Cuts at: 1996 2244 3474 4245 1996
Size: 248 1230 771 2313
Fragments arranged by size:
2313 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 160 944 976 1042 1074 2404 2434 2436
Size: 32 784 32 66 32 1330 30 2
Cuts at: 2436 2466 128
Size: 30 2224
Fragments arranged by size:
2224 1330 784 66 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1601 1601
Size: 4562
KasI G'GCGC_C
Cuts at: 1992 2240 1992
Size: 248 4314
KpnI G_GTAC'C
Cuts at: 376 1582 376
Size: 1206 3356
MluI A'CGCG_T
Cuts at: 3995 3995
Size: 4562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 362 3321 3505 362
Size: 2959 184 1419
Fragments arranged by size:
2959 1419 184
MscI TGG'CCA
Cuts at: 238 1483 2323 238
Size: 1245 840 2477
Fragments arranged by size:
2477 1245 840
MslI CAynn'nnrTG
Cuts at: 1363 1469 2551 2678 1363
Size: 106 1082 127 3247
Fragments arranged by size:
3247 1082 127 106
MspA1I CmG'CkG
Cuts at: 696 1300 1446 1673 1987 2347 3131 3376 696
Size: 604 146 227 314 360 784 245 1882
Fragments arranged by size:
1882 784 604 360 314 245 227 146
NaeI GCC'GGC
Cuts at: 2743 2743
Size: 4562
NarI GG'CG_CC
Cuts at: 1993 2241 1993
Size: 248 4314
NcoI C'CATG_G
Cuts at: 107 1521 107
Size: 1414 3148
NgoMIV G'CCGG_C
Cuts at: 2741 2741
Size: 4562
NheI G'CTAG_C
Cuts at: 1006 1006
Size: 4562
NsiI A_TGCA'T
Cuts at: 1245 1245
Size: 4562
NspI r_CATG'y
Cuts at: 693 1615 2115 3718 693
Size: 922 500 1603 1537
Fragments arranged by size:
1603 1537 922 500
PciI A'CATG_T
Cuts at: 3714 3714
Size: 4562
PfoI T'CCnGG_A
Cuts at: 1064 1064
Size: 4562
PmeI GTTT'AAAC
Cuts at: 1561 1561
Size: 4562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 4530
PpuMI rG'GwC_Cy
Cuts at: 1024 1024
Size: 4562
PspOMI G'GGCC_C
Cuts at: 509 509
Size: 4562
PstI C_TGCA'G
Cuts at: 1609 2294 1609
Size: 685 3877
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4562
PvuII CAG'CTG
Cuts at: 696 1300 1987 2347 696
Size: 604 687 360 2911
Fragments arranged by size:
2911 687 604 360
RsrII CG'GwC_CG
Cuts at: 2757 2757
Size: 4562
SacI G_AGCT'C
Cuts at: 1576 1576
Size: 4562
SalI G'TCGA_C
Cuts at: 1599 1599
Size: 4562
SanDI GG'GwC_CC
Cuts at: 1024 1024
Size: 4562
SapI GCTCTTCn'nnn_
Cuts at: 2585 2795 2585
Size: 210 4352
SbfI CC_TGCA'GG
Cuts at: 1609 1609
Size: 4562
ScaI AGT'ACT
Cuts at: 4079 4079
Size: 4562
SfcI C'TryA_G
Cuts at: 33 1605 2290 3258 3449 33
Size: 1572 685 968 191 1146
Fragments arranged by size:
1572 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1994 2242 1994
Size: 248 4314
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4562
SmaI CCC'GGG
Cuts at: 1584 1584
Size: 4562
SmlI C'TyrA_G
Cuts at: 454 826 1115 1717 2632 3346 3608 454
Size: 372 289 602 915 714 262 1408
Fragments arranged by size:
1408 915 714 602 372 289 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4562
SphI G_CATG'C
Cuts at: 1615 2115 1615
Size: 500 4062
SspI AAT'ATT
Cuts at: 822 822
Size: 4562
StuI AGG'CCT
Cuts at: 763 763
Size: 4562
StyI C'CwwG_G
Cuts at: 107 461 579 1224 1521 1696 2050 107
Size: 354 118 645 297 175 354 2619
Fragments arranged by size:
2619 645 354 354 297 175 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 256 615 2145 2278 2737 2946 256
Size: 359 1530 133 459 209 1872
Fragments arranged by size:
1872 1530 459 359 209 133
TatI w'GTAC_w
Cuts at: 4077 4077
Size: 4562
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 245 277 4079 4111 245
Size: 32 3802 32 696
Fragments arranged by size:
3802 696 32 32
Tth111I GACn'n_nGTC
Cuts at: 2359 2359
Size: 4562
XbaI T'CTAG_A
Cuts at: 1593 1593
Size: 4562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 841 918 2057 841
Size: 77 1139 3346
Fragments arranged by size:
3346 1139 77
XmaI C'CCGG_G
Cuts at: 1582 1582
Size: 4562
XmnI GAAnn'nnTTC
Cuts at: 1060 1060
Size: 4562
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
BbsI BbvCI BcgI BseRI BsgI BsiWI BsmBI BspEI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI PacI
PasI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI