Restriction map for
pF1KB5456
(Circular) (Six-base) MAPSORT of: pF1KB5456.seq Check: 114 from: 1 to: 4370
pF1KB5456 4370 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 236 690 789 236
Size: 454 99 3817
Fragments arranged by size:
3817 454 99
AatII G_ACGT'C
Cuts at: 550 550
Size: 4370
AccI GT'mk_AC
Cuts at: 1408 1408
Size: 4370
Acc65I G'GTAC_C
Cuts at: 1386 1386
Size: 4370
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 469 511 979 1030 1156 2195 2627 2980
Size: 251 42 468 51 126 1039 432 353
Cuts at: 2980 3642 218
Size: 662 946
Fragments arranged by size:
1039 946 662 468 432 353 251 126
51 42
AfeI AGC'GCT
Cuts at: 265 331 265
Size: 66 4304
AflIII A'CryG_T
Cuts at: 1344 1722 3522 3803 1344
Size: 378 1800 281 1911
Fragments arranged by size:
1911 1800 378 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 364 396 364
Size: 32 4338
AlwNI CAG_nnn'CTG
Cuts at: 321 471 615 1675 3113 321
Size: 150 144 1060 1438 1578
Fragments arranged by size:
1578 1438 1060 150 144
ApaI G_GGCC'C
Cuts at: 630 630
Size: 4370
ApaLI G'TGCA_C
Cuts at: 3208 3208
Size: 4370
ApoI r'AATT_y
Cuts at: 1374 3723 3979 1374
Size: 2349 256 1765
Fragments arranged by size:
2349 1765 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 556 629 1061 1273 1390 556
Size: 73 432 212 117 3536
Fragments arranged by size:
3536 432 212 117 73
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4370
BanI G'GyrC_C
Cuts at: 116 1386 1800 2048 2083 116
Size: 1270 414 248 35 2403
Fragments arranged by size:
2403 1270 414 248 35
BanII G_rGCy'C
Cuts at: 290 630 1091 1270 1384 2414 290
Size: 340 461 179 114 1030 2246
Fragments arranged by size:
2246 1030 461 340 179 114
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4122
BbvCI CC'TCA_GC
Cuts at: 515 515
Size: 4370
BciVI GTATCCnnnnn_n'
Cuts at: 2268 3324 4370 2268
Size: 1056 1046 2268
Fragments arranged by size:
2268 1056 1046
BclI T'GATC_A
Cuts at: 1890 1890
Size: 4370
BglI GCCn_nnn'nGGC
Cuts at: 634 922 931 634
Size: 288 9 4073
Fragments arranged by size:
4073 288 9
BglII A'GATC_T
Cuts at: 891 1885 3922 891
Size: 994 2037 1339
Fragments arranged by size:
2037 1339 994
BlpI GC'TnA_GC
Cuts at: 1482 1482
Size: 4370
Bme1580I G_kGCm'C
Cuts at: 630 1995 2088 3212 630
Size: 1365 93 1124 1788
Fragments arranged by size:
1788 1365 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 149 196 786 1582 1663 1998 4142 149
Size: 47 590 796 81 335 2144 377
Fragments arranged by size:
2144 796 590 377 335 81 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 292 454 637 679 892 1126 244
Size: 48 162 183 42 213 234 3488
Fragments arranged by size:
3488 234 213 183 162 48 42
Bpu10I CC'TnA_GC
Cuts at: 515 515
Size: 4370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1546 2425 3139 3437 1546
Size: 879 714 298 2479
Fragments arranged by size:
2479 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 247 988 1200 4074 247
Size: 741 212 2874 543
Fragments arranged by size:
2874 741 543 212
BsaAI yAC'GTr
Cuts at: 1723 1723
Size: 4370
BsaBI GATnn'nnATC
Cuts at: 6 1884 3921 6
Size: 1878 2037 455
Fragments arranged by size:
2037 1878 455
BsaHI Gr'CG_yC
Cuts at: 547 1801 2049 2751 4247 547
Size: 1254 248 702 1496 670
Fragments arranged by size:
1496 1254 702 670 248
BsaWI w'CCGG_w
Cuts at: 1002 1559 2080 2758 3169 3316 3590 1002
Size: 557 521 678 411 147 274 1782
Fragments arranged by size:
1782 678 557 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 364 394 890 920 364
Size: 30 496 30 3814
Fragments arranged by size:
3814 496 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 191 289 352 385 787 934 979 1224 1275
Size: 98 63 33 402 147 45 245 51
Cuts at: 1275 1278 1291 191
Size: 3 13 3270
Fragments arranged by size:
3270 402 245 147 98 63 51 45
33 13 3
BseYI C'CCAG_C
Cuts at: 1252 1795 2553 3218 3626 1252
Size: 543 758 665 408 1996
Fragments arranged by size:
1996 758 665 543 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 208 718 169
Size: 39 510 3821
Fragments arranged by size:
3821 510 39
BsiEI CG_ry'CG
Cuts at: 104 1912 1958 3188 104
Size: 1808 46 1230 1286
Fragments arranged by size:
1808 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 290 488 1091 1384 2162 2352 3212 290
Size: 198 603 293 778 190 860 1448
Fragments arranged by size:
1448 860 778 603 293 198 190
BsmI GAATG_Cn'
Cuts at: 610 884 610
Size: 274 4096
Bsp1286I G_dGCh'C
Cuts at: 290 488 630 1091 1270 1384 1995 2088 2162
Size: 198 142 461 179 114 611 93 74
Cuts at: 2162 2352 2414 3212 290
Size: 190 62 798 1448
Fragments arranged by size:
1448 798 611 461 198 190 179 142
114 93 74 62
BspHI T'CATG_A
Cuts at: 2802 2802
Size: 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 690 789 1420 1634 1936 2317 236
Size: 454 99 631 214 302 381 2289
Fragments arranged by size:
2289 631 454 381 302 214 99
BsrBI CCG'CTC
Cuts at: 50 867 2662 2716 4200 4365 50
Size: 817 1795 54 1484 165 55
Fragments arranged by size:
1795 1484 817 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2282 2282
Size: 4370
BsrFI r'CCGG_y
Cuts at: 2368 2549 2368
Size: 181 4189
BssSI C'ACGA_G
Cuts at: 2641 3349 2641
Size: 708 3662
BstAPI GCAn_nnn'nTGC
Cuts at: 471 696 1747 471
Size: 225 1051 3094
Fragments arranged by size:
3094 1051 225
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstXI CCAn_nnnn'nTGG
Cuts at: 414 414
Size: 4370
BstYI r'GATC_y
Cuts at: 1 891 1395 1862 1885 2220 2466 2772 2784
Size: 890 504 467 23 335 246 306 12
Cuts at: 2784 2870 3922 1
Size: 86 1052 449
Fragments arranged by size:
1052 890 504 467 449 335 306 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2320 2501 2664 2320
Size: 181 163 4026
Fragments arranged by size:
4026 181 163
BtsI GCAGTG_nn'
Cuts at: 867 1613 867
Size: 746 3624
ClaI AT'CG_AT
Cuts at: 950 950
Size: 4370
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4370
DraIII CAC_nnn'GTG
Cuts at: 696 696
Size: 4370
DrdI GACnn_nn'nnGTC
Cuts at: 2076 3420 2076
Size: 1344 3026
EaeI y'GGCC_r
Cuts at: 635 932 1073 1157 1955 2129 2520 2547 3892
Size: 297 141 84 798 174 391 27 1345
Cuts at: 3892 635
Size: 1113
Fragments arranged by size:
1345 1113 798 391 297 174 141 84
27
EagI C'GGCC_G
Cuts at: 1955 1955
Size: 4370
EarI CTCTTCn'nnn_
Cuts at: 121 188 445 487 772 823 2393 2603 121
Size: 67 257 42 285 51 1570 210 1888
Fragments arranged by size:
1888 1570 285 257 210 67 51 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1036 1051 2578 3320 3466 4180 1036
Size: 15 1527 742 146 714 1226
Fragments arranged by size:
1527 1226 742 714 146 15
EcoICRI GAG'CTC
Cuts at: 288 1089 1382 288
Size: 801 293 3276
Fragments arranged by size:
3276 801 293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 244 292 454 469 511 637 679 892
Size: 26 48 162 15 42 126 42 213
Cuts at: 892 979 1030 1126 1156 2195 2627 2980 3642
Size: 87 51 96 30 1039 432 353 662
Cuts at: 3642 218
Size: 946
Fragments arranged by size:
1039 946 662 432 353 213 162 126
96 87 51 48 42 42 30 26
15
EcoO109I rG'GnC_Cy
Cuts at: 924 1203 1317 1509 924
Size: 279 114 192 3785
Fragments arranged by size:
3785 279 192 114
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
EcoRV GAT'ATC
Cuts at: 1026 1026
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1056 2389 1056
Size: 1333 3037
FspI TGC'GCA
Cuts at: 715 2151 715
Size: 1436 2934
HaeII r_GCGC'y
Cuts at: 248 267 333 679 759 1804 2052 3282 4053
Size: 19 66 346 80 1045 248 1230 771
Cuts at: 4053 248
Size: 565
Fragments arranged by size:
1230 1045 771 565 346 248 80 66
19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 284 316 511 543 564 596 2212 2242 2244
Size: 32 195 32 21 32 1616 30 2
Cuts at: 2244 2274 284
Size: 30 2380
Fragments arranged by size:
2380 1616 195 32 32 32 30 30
21 2
HincII GTy'rAC
Cuts at: 1409 1409
Size: 4370
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4122
KpnI G_GTAC'C
Cuts at: 1390 1390
Size: 4370
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3129 3313 3129
Size: 184 4186
MscI TGG'CCA
Cuts at: 934 2131 934
Size: 1197 3173
MslI CAynn'nnrTG
Cuts at: 2359 2486 2359
Size: 127 4243
MspA1I CmG'CkG
Cuts at: 657 711 885 1104 1113 1145 1481 1795 2155
Size: 54 174 219 9 32 336 314 360
Cuts at: 2155 2939 3184 657
Size: 784 245 1843
Fragments arranged by size:
1843 784 360 336 314 245 219 174
54 32 9
NaeI GCC'GGC
Cuts at: 2551 2551
Size: 4370
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4122
NgoMIV G'CCGG_C
Cuts at: 2549 2549
Size: 4370
NspI r_CATG'y
Cuts at: 720 1189 1348 1423 1923 3526 720
Size: 469 159 75 500 1603 1564
Fragments arranged by size:
1603 1564 500 469 159 75
PciI A'CATG_T
Cuts at: 1344 3522 1344
Size: 2178 2192
PflMI CCAn_nnn'nTGG
Cuts at: 615 732 615
Size: 117 4253
PfoI T'CCnGG_A
Cuts at: 398 398
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 364 396 2816 2848 364
Size: 32 2420 32 1886
Fragments arranged by size:
2420 1886 32 32
PspOMI G'GGCC_C
Cuts at: 626 626
Size: 4370
PstI C_TGCA'G
Cuts at: 569 683 1097 1118 1340 1417 2102 569
Size: 114 414 21 222 77 685 2837
Fragments arranged by size:
2837 685 414 222 114 77 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 657 711 885 1104 1113 1145 1795 2155 657
Size: 54 174 219 9 32 650 360 2872
Fragments arranged by size:
2872 650 360 219 174 54 32 9
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 290 1091 1384 290
Size: 801 293 3276
Fragments arranged by size:
3276 801 293
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 121 823 2393 2603 121
Size: 702 1570 210 1888
Fragments arranged by size:
1888 1570 702 210
SbfI CC_TGCA'GG
Cuts at: 1097 1417 1097
Size: 320 4050
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SfcI C'TryA_G
Cuts at: 33 565 679 1093 1114 1336 1413 2098 3066
Size: 532 114 414 21 222 77 685 968
Cuts at: 3066 3257 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 532 414 222 191 114
77 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 634 922 931 634
Size: 288 9 4073
Fragments arranged by size:
4073 288 9
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 631 1063 1392 631
Size: 432 329 3609
Fragments arranged by size:
3609 432 329
SmlI C'TyrA_G
Cuts at: 1525 2440 3154 3416 1525
Size: 915 714 262 2479
Fragments arranged by size:
2479 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 720 1423 1923 720
Size: 703 500 3167
Fragments arranged by size:
3167 703 500
SrfI GCCC'GGGC
Cuts at: 631 631
Size: 4370
StuI AGG'CCT
Cuts at: 526 526
Size: 4370
StyI C'CwwG_G
Cuts at: 1504 1858 1504
Size: 354 4016
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1953 2086 2545 2754 1953
Size: 133 459 209 3569
Fragments arranged by size:
3569 459 209 133
TatI w'GTAC_w
Cuts at: 1124 3885 1124
Size: 2761 1609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4338
Tth111I GACn'n_nGTC
Cuts at: 2167 2167
Size: 4370
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 414 1865 414
Size: 1451 2919
XmaI C'CCGG_G
Cuts at: 629 1061 1390 629
Size: 432 329 3609
Fragments arranged by size:
3609 432 329
XmnI GAAnn'nnTTC
Cuts at: 379 379
Size: 4370
ZraI GAC'GTC
Cuts at: 548 548
Size: 4370
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AlfI AscI AvrII BaeI
BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BspEI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI CspCI EcoNI
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SspI SwaI XhoI