Restriction map for
pF1KB5454
(Circular) (Six-base) MAPSORT of: pF1KB5454.seq Check: 1427 from: 1 to: 4655
pF1KB5454 4655 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 1607 1607
Size: 4655
AccI GT'mk_AC
Cuts at: 1000 1693 1000
Size: 693 3962
Acc65I G'GTAC_C
Cuts at: 1671 1671
Size: 4655
AclI AA'CG_TT
Cuts at: 4495 4495
Size: 4655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 472 2480 2912 3659 3927 295
Size: 177 2008 432 747 268 1023
Fragments arranged by size:
2008 1023 747 432 268 177
AfeI AGC'GCT
Cuts at: 184 184
Size: 4655
AflIII A'CryG_T
Cuts at: 2007 3111 4088 2007
Size: 1104 977 2574
Fragments arranged by size:
2574 1104 977
AgeI A'CCGG_T
Cuts at: 3043 3043
Size: 4655
AhdI GACnn_n'nnGTC
Cuts at: 1113 1113
Size: 4655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 950 1477 950
Size: 527 4128
AlwNI CAG_nnn'CTG
Cuts at: 1170 1960 3527 1170
Size: 790 1567 2298
Fragments arranged by size:
2298 1567 790
ApaLI G'TGCA_C
Cuts at: 3425 3425
Size: 4655
ApoI r'AATT_y
Cuts at: 1659 4008 4264 1659
Size: 2349 256 2050
Fragments arranged by size:
2349 2050 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4655
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
AvaI C'yCGr_G
Cuts at: 195 1675 195
Size: 1480 3175
BamHI G'GATC_C
Cuts at: 1680 1680
Size: 4655
BanI G'GyrC_C
Cuts at: 1671 2085 2333 2368 1671
Size: 414 248 35 3958
Fragments arranged by size:
3958 414 248 35
BanII G_rGCy'C
Cuts at: 1027 1264 1669 2699 1027
Size: 237 405 1030 2983
Fragments arranged by size:
2983 1030 405 237
BbeI G_GCGC'C
Cuts at: 2089 2337 2089
Size: 248 4407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 279 313 279
Size: 34 4621
BciVI GTATCCnnnnn_n'
Cuts at: 2553 3314 4655 2553
Size: 761 1341 2553
Fragments arranged by size:
2553 1341 761
BclI T'GATC_A
Cuts at: 2175 2175
Size: 4655
BglI GCCn_nnn'nGGC
Cuts at: 670 670
Size: 4655
BglII A'GATC_T
Cuts at: 2170 4207 2170
Size: 2037 2618
BlpI GC'TnA_GC
Cuts at: 826 1767 826
Size: 941 3714
Bme1580I G_kGCm'C
Cuts at: 2280 2373 3429 2280
Size: 93 1056 3506
Fragments arranged by size:
3506 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1867 1948 2283 4427 1867
Size: 81 335 2144 2095
Fragments arranged by size:
2144 2095 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1041 1302 3022 1041
Size: 261 1720 2674
Fragments arranged by size:
2674 1720 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 1450 1264
Size: 186 4469
Bpu10I CC'TnA_GC
Cuts at: 1263 1263
Size: 4655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2710 3202 3500 1831
Size: 879 492 298 2986
Fragments arranged by size:
2986 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1290 4359 1290
Size: 3069 1586
BsaAI yAC'GTr
Cuts at: 2008 2008
Size: 4655
BsaBI GATnn'nnATC
Cuts at: 6 2169 4206 6
Size: 2163 2037 455
Fragments arranged by size:
2163 2037 455
BsaHI Gr'CG_yC
Cuts at: 1604 2086 2334 3036 4532 1604
Size: 482 248 702 1496 1727
Fragments arranged by size:
1727 1496 702 482 248
BsaWI w'CCGG_w
Cuts at: 1844 2365 3043 3317 3464 3875 1844
Size: 521 678 274 147 411 2624
Fragments arranged by size:
2624 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1237 1267 1237
Size: 30 4625
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 421 1168 421
Size: 747 3908
BseYI C'CCAG_C
Cuts at: 153 673 2080 2838 3415 3911 153
Size: 520 1407 758 577 496 897
Fragments arranged by size:
1407 897 758 577 520 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 928 508
Size: 420 4235
BsiEI CG_ry'CG
Cuts at: 104 967 2197 2243 3451 104
Size: 863 1230 46 1208 1308
Fragments arranged by size:
1308 1230 1208 863 46
BsiHKAI G_wGCw'C
Cuts at: 1669 2447 2637 3429 1669
Size: 778 190 792 2895
Fragments arranged by size:
2895 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 163 1595 163
Size: 1432 3223
Bsp1286I G_dGCh'C
Cuts at: 1027 1264 1669 2280 2373 2447 2637 2699 3429
Size: 237 405 611 93 74 190 62 730
Cuts at: 3429 1027
Size: 2253
Fragments arranged by size:
2253 730 611 405 237 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3875 3875
Size: 4655
BspHI T'CATG_A
Cuts at: 791 3831 791
Size: 3040 1615
BspMI ACCTGCnnnn'nnnn_
Cuts at: 983 1012 1705 1919 2221 2602 983
Size: 29 693 214 302 381 3036
Fragments arranged by size:
3036 693 381 302 214 29
BsrBI CCG'CTC
Cuts at: 50 468 489 2947 3001 4485 4650 50
Size: 418 21 2458 54 1484 165 55
Fragments arranged by size:
2458 1484 418 165 55 54 21
BsrDI GCAATG_nn'
Cuts at: 932 2567 932
Size: 1635 3020
BsrFI r'CCGG_y
Cuts at: 1319 2653 2834 3043 1319
Size: 1334 181 209 2931
Fragments arranged by size:
2931 1334 209 181
BsrGI T'GTAC_A
Cuts at: 592 592
Size: 4655
BssSI C'ACGA_G
Cuts at: 2926 3284 2926
Size: 358 4297
BstAPI GCAn_nnn'nTGC
Cuts at: 2032 2032
Size: 4655
BstBI TT'CG_AA
Cuts at: 1326 3016 1326
Size: 1690 2965
BstEII G'GTnAC_C
Cuts at: 1638 1638
Size: 4655
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4655
BstYI r'GATC_y
Cuts at: 1 117 1680 2147 2170 2505 2751 3763 3849
Size: 116 1563 467 23 335 246 1012 86
Cuts at: 3849 3861 4207 1
Size: 12 346 449
Fragments arranged by size:
1563 1012 467 449 346 335 246 116
86 23 12
BtgI C'CryG_G
Cuts at: 107 665 1029 1044 107
Size: 558 364 15 3718
Fragments arranged by size:
3718 558 364 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2605 2786 2949 2605
Size: 181 163 4311
Fragments arranged by size:
4311 181 163
BtsI GCAGTG_nn'
Cuts at: 876 1898 876
Size: 1022 3633
DraI TTT'AAA
Cuts at: 1654 1654
Size: 4655
DraIII CAC_nnn'GTG
Cuts at: 1191 1191
Size: 4655
DrdI GACnn_nn'nnGTC
Cuts at: 2361 3219 2361
Size: 858 3797
EaeI y'GGCC_r
Cuts at: 964 1032 2240 2414 2805 2832 4177 964
Size: 68 1208 174 391 27 1345 1442
Fragments arranged by size:
1442 1345 1208 391 174 68 27
EagI C'GGCC_G
Cuts at: 964 2240 964
Size: 1276 3379
EarI CTCTTCn'nnn_
Cuts at: 1127 2678 2888 1127
Size: 1551 210 2894
Fragments arranged by size:
2894 1551 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2863 3173 3319 4465 2863
Size: 310 146 1146 3053
Fragments arranged by size:
3053 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1667 1667
Size: 4655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 472 1264 1450 2480 2912 3659 3927 295
Size: 177 792 186 1030 432 747 268 1023
Fragments arranged by size:
1030 1023 792 747 432 268 186 177
EcoNI CCTnn'n_nnAGG
Cuts at: 718 718
Size: 4655
EcoO109I rG'GnC_Cy
Cuts at: 150 192 702 972 1794 150
Size: 42 510 270 822 3011
Fragments arranged by size:
3011 822 510 270 42
EcoRI G'AATT_C
Cuts at: 1659 1659
Size: 4655
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 4655
FspI TGC'GCA
Cuts at: 2436 2436
Size: 4655
HaeII r_GCGC'y
Cuts at: 186 2089 2337 3359 4338 186
Size: 1903 248 1022 979 503
Fragments arranged by size:
1903 1022 979 503 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 325 357 874 906 1099 1131 2497 2527 2529
Size: 32 517 32 193 32 1366 30 2
Cuts at: 2529 2559 325
Size: 30 2421
Fragments arranged by size:
2421 1366 517 193 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1694 1694
Size: 4655
HindIII A'AGCT_T
Cuts at: 1234 1234
Size: 4655
KasI G'GCGC_C
Cuts at: 2085 2333 2085
Size: 248 4407
KpnI G_GTAC'C
Cuts at: 1675 1675
Size: 4655
MluI A'CGCG_T
Cuts at: 4088 4088
Size: 4655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 450 458 3326 3510 450
Size: 8 2868 184 1595
Fragments arranged by size:
2868 1595 184 8
MscI TGG'CCA
Cuts at: 2416 2416
Size: 4655
MslI CAynn'nnrTG
Cuts at: 950 1034 1459 2644 2771 950
Size: 84 425 1185 127 2834
Fragments arranged by size:
2834 1185 425 127 84
MspA1I CmG'CkG
Cuts at: 213 1766 2080 2440 3453 3698 213
Size: 1553 314 360 1013 245 1170
Fragments arranged by size:
1553 1170 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1321 2836 1321
Size: 1515 3140
NarI GG'CG_CC
Cuts at: 2086 2334 2086
Size: 248 4407
NcoI C'CATG_G
Cuts at: 107 1029 107
Size: 922 3733
NdeI CA'TA_TG
Cuts at: 1379 1379
Size: 4655
NgoMIV G'CCGG_C
Cuts at: 1319 2834 1319
Size: 1515 3140
NspI r_CATG'y
Cuts at: 1708 2208 3115 1708
Size: 500 907 3248
Fragments arranged by size:
3248 907 500
PasI CC'CwG_GG
Cuts at: 1170 1170
Size: 4655
PciI A'CATG_T
Cuts at: 3111 3111
Size: 4655
PflMI CCAn_nnn'nTGG
Cuts at: 1170 1170
Size: 4655
PfoI T'CCnGG_A
Cuts at: 713 1427 713
Size: 714 3941
PmeI GTTT'AAAC
Cuts at: 1654 1654
Size: 4655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 710 742 3794 3826 710
Size: 32 3052 32 1539
Fragments arranged by size:
3052 1539 32 32
PpuMI rG'GwC_Cy
Cuts at: 150 192 702 972 150
Size: 42 510 270 3833
Fragments arranged by size:
3833 510 270 42
PsiI TTA'TAA
Cuts at: 851 851
Size: 4655
PstI C_TGCA'G
Cuts at: 384 1628 1702 2387 384
Size: 1244 74 685 2652
Fragments arranged by size:
2652 1244 685 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4655
PvuII CAG'CTG
Cuts at: 213 2080 2440 213
Size: 1867 360 2428
Fragments arranged by size:
2428 1867 360
RsrII CG'GwC_CG
Cuts at: 2850 2850
Size: 4655
SacI G_AGCT'C
Cuts at: 1669 1669
Size: 4655
SalI G'TCGA_C
Cuts at: 1692 1692
Size: 4655
SanDI GG'GwC_CC
Cuts at: 150 150
Size: 4655
SapI GCTCTTCn'nnn_
Cuts at: 2678 2888 2678
Size: 210 4445
SbfI CC_TGCA'GG
Cuts at: 1702 1702
Size: 4655
ScaI AGT'ACT
Cuts at: 610 4172 610
Size: 3562 1093
SexAI A'CCwGG_T
Cuts at: 623 623
Size: 4655
SfcI C'TryA_G
Cuts at: 33 380 1624 1698 2383 3376 3567 33
Size: 347 1244 74 685 993 191 1121
Fragments arranged by size:
1244 1121 993 685 347 191 74
SfoI GGC'GCC
Cuts at: 2087 2335 2087
Size: 248 4407
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
SmaI CCC'GGG
Cuts at: 197 1677 197
Size: 1480 3175
SmlI C'TyrA_G
Cuts at: 1810 2725 3217 3479 1810
Size: 915 492 262 2986
Fragments arranged by size:
2986 915 492 262
SpeI A'CTAG_T
Cuts at: 67 201 67
Size: 134 4521
SphI G_CATG'C
Cuts at: 1708 2208 1708
Size: 500 4155
SspI AAT'ATT
Cuts at: 1197 1197
Size: 4655
StuI AGG'CCT
Cuts at: 1249 1249
Size: 4655
StyI C'CwwG_G
Cuts at: 107 749 1029 1789 2143 107
Size: 642 280 760 354 2619
Fragments arranged by size:
2619 760 642 354 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2238 2371 2830 3039 2238
Size: 133 459 209 3854
Fragments arranged by size:
3854 459 209 133
TatI w'GTAC_w
Cuts at: 592 608 1091 4170 592
Size: 16 483 3079 1077
Fragments arranged by size:
3079 1077 483 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 570 602 4172 4204 570
Size: 32 3570 32 1021
Fragments arranged by size:
3570 1021 32 32
Tth111I GACn'n_nGTC
Cuts at: 2452 2452
Size: 4655
XbaI T'CTAG_A
Cuts at: 1686 1686
Size: 4655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1353 2150 1353
Size: 797 3858
XmaI C'CCGG_G
Cuts at: 195 1675 195
Size: 1480 3175
XmnI GAAnn'nnTTC
Cuts at: 184 1480 184
Size: 1296 3359
ZraI GAC'GTC
Cuts at: 1605 1605
Size: 4655
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AflII AleI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BmgBI BmtI BsiWI BsmI BssHII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PmlI PshAI
PspOMI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SwaI XhoI