Restriction map for
pF1KB5452
(Circular) (Six-base) MAPSORT of: pF1KB5452.seq Check: 9952 from: 1 to: 4379
pF1KB5452 4379 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1417 1417
Size: 4379
Acc65I G'GTAC_C
Cuts at: 1395 1395
Size: 4379
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 97 928 2204 2636 3383 3651 97
Size: 831 1276 432 747 268 825
Fragments arranged by size:
1276 831 825 747 432 268
AflII C'TTAA_G
Cuts at: 684 684
Size: 4379
AflIII A'CryG_T
Cuts at: 869 1371 1731 2835 3812 869
Size: 502 360 1104 977 1436
Fragments arranged by size:
1436 1104 977 502 360
AgeI A'CCGG_T
Cuts at: 2767 2767
Size: 4379
AlwNI CAG_nnn'CTG
Cuts at: 589 1684 3251 589
Size: 1095 1567 1717
Fragments arranged by size:
1717 1567 1095
ApaLI G'TGCA_C
Cuts at: 3149 3149
Size: 4379
ApoI r'AATT_y
Cuts at: 501 1383 3732 3988 501
Size: 882 2349 256 892
Fragments arranged by size:
2349 892 882 256
AseI AT'TA_AT
Cuts at: 20 527 20
Size: 507 3872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 1399 1399
Size: 4379
BamHI G'GATC_C
Cuts at: 1404 1404
Size: 4379
BanI G'GyrC_C
Cuts at: 1395 1809 2057 2092 1395
Size: 414 248 35 3682
Fragments arranged by size:
3682 414 248 35
BanII G_rGCy'C
Cuts at: 1393 2423 1393
Size: 1030 3349
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4131
BbsI GAAGACnn'nnnn_
Cuts at: 132 1029 132
Size: 897 3482
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4345
BciVI GTATCCnnnnn_n'
Cuts at: 1117 2277 3038 4379 1117
Size: 1160 761 1341 1117
Fragments arranged by size:
1341 1160 1117 761
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4379
BglII A'GATC_T
Cuts at: 915 1071 1894 3931 915
Size: 156 823 2037 1363
Fragments arranged by size:
2037 1363 823 156
BlpI GC'TnA_GC
Cuts at: 1491 1491
Size: 4379
Bme1580I G_kGCm'C
Cuts at: 2004 2097 3153 2004
Size: 93 1056 3230
Fragments arranged by size:
3230 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 825 1591 1672 2007 4151 825
Size: 766 81 335 2144 1053
Fragments arranged by size:
2144 1053 766 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1088 463
Size: 625 3754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 2434 2926 3224 1555
Size: 879 492 298 2710
Fragments arranged by size:
2710 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 173 4083 173
Size: 3910 469
BsaAI yAC'GTr
Cuts at: 776 1732 776
Size: 956 3423
BsaBI GATnn'nnATC
Cuts at: 6 1893 3930 6
Size: 1887 2037 455
Fragments arranged by size:
2037 1887 455
BsaHI Gr'CG_yC
Cuts at: 1810 2058 2760 4256 1810
Size: 248 702 1496 1933
Fragments arranged by size:
1933 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1568 2089 2767 3041 3188 3599 1568
Size: 521 678 274 147 411 2348
Fragments arranged by size:
2348 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 322 352 322
Size: 30 4349
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 135 135
Size: 4379
BseYI C'CCAG_C
Cuts at: 1804 2562 3139 3635 1804
Size: 758 577 496 2548
Fragments arranged by size:
2548 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1921 1967 3175 104
Size: 1817 46 1208 1308
Fragments arranged by size:
1817 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1393 2171 2361 3153 1393
Size: 778 190 792 2619
Fragments arranged by size:
2619 792 778 190
BsmI GAATG_Cn'
Cuts at: 162 162
Size: 4379
Bsp1286I G_dGCh'C
Cuts at: 1393 2004 2097 2171 2361 2423 3153 1393
Size: 611 93 74 190 62 730 2619
Fragments arranged by size:
2619 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3599 3599
Size: 4379
BspHI T'CATG_A
Cuts at: 243 744 3555 243
Size: 501 2811 1067
Fragments arranged by size:
2811 1067 501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1340 1429 1643 1945 2326 1340
Size: 89 214 302 381 3393
Fragments arranged by size:
3393 381 302 214 89
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4209 4374 50
Size: 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 639 2291 639
Size: 1652 2727
BsrFI r'CCGG_y
Cuts at: 2377 2558 2767 2377
Size: 181 209 3989
Fragments arranged by size:
3989 209 181
BssSI C'ACGA_G
Cuts at: 2650 3008 2650
Size: 358 4021
BstAPI GCAn_nnn'nTGC
Cuts at: 1756 1756
Size: 4379
BstBI TT'CG_AA
Cuts at: 2740 2740
Size: 4379
BstEII G'GTnAC_C
Cuts at: 946 946
Size: 4379
BstYI r'GATC_y
Cuts at: 1 915 1071 1142 1404 1871 1894 2229 2475
Size: 914 156 71 262 467 23 335 246
Cuts at: 2475 3487 3573 3585 3931 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 914 467 449 346 335 262 246
156 86 71 23 12
Bsu36I CC'TnA_GG
Cuts at: 379 379
Size: 4379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2329 2510 2673 2329
Size: 181 163 4035
Fragments arranged by size:
4035 181 163
BtsI GCAGTG_nn'
Cuts at: 129 1622 129
Size: 1493 2886
DraI TTT'AAA
Cuts at: 1092 1378 1092
Size: 286 4093
DraIII CAC_nnn'GTG
Cuts at: 457 457
Size: 4379
DrdI GACnn_nn'nnGTC
Cuts at: 874 2085 2943 874
Size: 1211 858 2310
Fragments arranged by size:
2310 1211 858
EaeI y'GGCC_r
Cuts at: 1964 2138 2529 2556 3901 1964
Size: 174 391 27 1345 2442
Fragments arranged by size:
2442 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1964 1964
Size: 4379
EarI CTCTTCn'nnn_
Cuts at: 603 2402 2612 603
Size: 1799 210 2370
Fragments arranged by size:
2370 1799 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1099 2587 2897 3043 4189 1099
Size: 1488 310 146 1146 1289
Fragments arranged by size:
1488 1289 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1391 1391
Size: 4379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 97 463 928 1088 2204 2636 3383 3651 97
Size: 366 465 160 1116 432 747 268 825
Fragments arranged by size:
1116 825 747 465 432 366 268 160
EcoO109I rG'GnC_Cy
Cuts at: 1518 1518
Size: 4379
EcoRI G'AATT_C
Cuts at: 1383 1383
Size: 4379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4379
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4379
HaeII r_GCGC'y
Cuts at: 1813 2061 3083 4062 1813
Size: 248 1022 979 2130
Fragments arranged by size:
2130 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1152 1184 2221 2251 2253 2283 1152
Size: 32 1037 30 2 30 3248
Fragments arranged by size:
3248 1037 32 30 30 2
HincII GTy'rAC
Cuts at: 314 879 1418 314
Size: 565 539 3275
Fragments arranged by size:
3275 565 539
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4131
KpnI G_GTAC'C
Cuts at: 1399 1399
Size: 4379
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3050 3234 3050
Size: 184 4195
MscI TGG'CCA
Cuts at: 2140 2140
Size: 4379
MslI CAynn'nnrTG
Cuts at: 2368 2495 2368
Size: 127 4252
MspA1I CmG'CkG
Cuts at: 1490 1804 2164 3177 3422 1490
Size: 314 360 1013 245 2447
Fragments arranged by size:
2447 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4379
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4131
NdeI CA'TA_TG
Cuts at: 846 846
Size: 4379
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4379
NspI r_CATG'y
Cuts at: 873 1002 1375 1432 1932 2839 873
Size: 129 373 57 500 907 2413
Fragments arranged by size:
2413 907 500 373 129 57
PciI A'CATG_T
Cuts at: 869 1371 2835 869
Size: 502 1464 2413
Fragments arranged by size:
2413 1464 502
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1180 1212 3518 3550 1180
Size: 32 2306 32 2009
Fragments arranged by size:
2306 2009 32 32
PstI C_TGCA'G
Cuts at: 585 1426 2111 585
Size: 841 685 2853
Fragments arranged by size:
2853 841 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 1804 2164 1804
Size: 360 4019
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 1393 1393
Size: 4379
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 603 2402 2612 603
Size: 1799 210 2370
Fragments arranged by size:
2370 1799 210
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4379
ScaI AGT'ACT
Cuts at: 197 3896 197
Size: 3699 680
SexAI A'CCwGG_T
Cuts at: 648 648
Size: 4379
SfcI C'TryA_G
Cuts at: 33 581 1422 2107 3100 3291 33
Size: 548 841 685 993 191 1121
Fragments arranged by size:
1121 993 841 685 548 191
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4131
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4379
SmlI C'TyrA_G
Cuts at: 684 1534 2449 2941 3203 684
Size: 850 915 492 262 1860
Fragments arranged by size:
1860 915 850 492 262
SnaBI TAC'GTA
Cuts at: 776 776
Size: 4379
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 1432 1932 1432
Size: 500 3879
SspI AAT'ATT
Cuts at: 671 889 671
Size: 218 4161
StuI AGG'CCT
Cuts at: 384 440 384
Size: 56 4323
StyI C'CwwG_G
Cuts at: 1513 1867 1513
Size: 354 4025
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 299 984 1962 2095 2554 2763 299
Size: 685 978 133 459 209 1915
Fragments arranged by size:
1915 978 685 459 209 133
TatI w'GTAC_w
Cuts at: 195 3894 195
Size: 3699 680
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4347
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4379
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1874 1874
Size: 4379
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4379
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I
BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SrfI SwaI
XhoI XmnI ZraI