Restriction map for
pF1KB5451
(Circular) (Six-base) MAPSORT of: pF1KB5451.seq Check: 6001 from: 1 to: 4229
pF1KB5451 4229 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 154 154
Size: 4229
AatII G_ACGT'C
Cuts at: 577 577
Size: 4229
AccI GT'mk_AC
Cuts at: 248 1267 248
Size: 1019 3210
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 653 1165 2054 2486 3233 3501 223
Size: 430 512 889 432 747 268 951
Fragments arranged by size:
951 889 747 512 432 430 268
AflIII A'CryG_T
Cuts at: 1151 1581 2685 3662 1151
Size: 430 1104 977 1718
Fragments arranged by size:
1718 1104 977 430
AgeI A'CCGG_T
Cuts at: 2617 2617
Size: 4229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1210 1210
Size: 4229
AlwNI CAG_nnn'CTG
Cuts at: 696 1064 1534 3101 696
Size: 368 470 1567 1824
Fragments arranged by size:
1824 1567 470 368
ApaLI G'TGCA_C
Cuts at: 2999 2999
Size: 4229
ApoI r'AATT_y
Cuts at: 284 431 621 919 1233 3582 3838 284
Size: 147 190 298 314 2349 256 675
Fragments arranged by size:
2349 675 314 298 256 190 147
AseI AT'TA_AT
Cuts at: 20 20
Size: 4229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 339 1128 1249 339
Size: 789 121 3319
Fragments arranged by size:
3319 789 121
BamHI G'GATC_C
Cuts at: 1254 1254
Size: 4229
BanI G'GyrC_C
Cuts at: 238 1245 1659 1907 1942 238
Size: 1007 414 248 35 2525
Fragments arranged by size:
2525 1007 414 248 35
BanII G_rGCy'C
Cuts at: 380 955 1243 2273 380
Size: 575 288 1030 2336
Fragments arranged by size:
2336 1030 575 288
BbeI G_GCGC'C
Cuts at: 242 1663 1911 242
Size: 1421 248 2560
Fragments arranged by size:
2560 1421 248
BbsI GAAGACnn'nnnn_
Cuts at: 659 659
Size: 4229
BciVI GTATCCnnnnn_n'
Cuts at: 2127 2888 4229 2127
Size: 761 1341 2127
Fragments arranged by size:
2127 1341 761
BclI T'GATC_A
Cuts at: 1749 1749
Size: 4229
BglI GCCn_nnn'nGGC
Cuts at: 728 728
Size: 4229
BglII A'GATC_T
Cuts at: 1744 3781 1744
Size: 2037 2192
BlpI GC'TnA_GC
Cuts at: 1341 1341
Size: 4229
Bme1580I G_kGCm'C
Cuts at: 1854 1947 3003 1854
Size: 93 1056 3080
Fragments arranged by size:
3080 1056 93
BmgBI CAC'GTC
Cuts at: 527 527
Size: 4229
BmrI ACTGGGnnnn_n'
Cuts at: 1441 1522 1857 4001 1441
Size: 81 335 2144 1669
Fragments arranged by size:
2144 1669 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 2284 2776 3074 1405
Size: 879 492 298 2560
Fragments arranged by size:
2560 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 976 3933 976
Size: 2957 1272
BsaAI yAC'GTr
Cuts at: 1194 1582 1194
Size: 388 3841
BsaBI GATnn'nnATC
Cuts at: 6 1743 3780 6
Size: 1737 2037 455
Fragments arranged by size:
2037 1737 455
BsaHI Gr'CG_yC
Cuts at: 239 419 574 1217 1660 1908 2610 4106 239
Size: 180 155 643 443 248 702 1496 362
Fragments arranged by size:
1496 702 643 443 362 248 180 155
BsaWI w'CCGG_w
Cuts at: 224 1418 1939 2617 2891 3038 3449 224
Size: 1194 521 678 274 147 411 1004
Fragments arranged by size:
1194 1004 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 388
Size: 4229
BseYI C'CCAG_C
Cuts at: 814 1051 1654 2412 2989 3485 814
Size: 237 603 758 577 496 1558
Fragments arranged by size:
1558 758 603 577 496 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 1216
Size: 4229
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3025 104
Size: 1667 46 1208 1308
Fragments arranged by size:
1667 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 380 1243 2021 2211 3003 380
Size: 863 778 190 792 1606
Fragments arranged by size:
1606 863 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 380 955 1243 1854 1947 2021 2211 2273 3003
Size: 575 288 611 93 74 190 62 730
Cuts at: 3003 380
Size: 1606
Fragments arranged by size:
1606 730 611 575 288 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3449 3449
Size: 4229
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 154 1279 1493 1795 2176 154
Size: 1125 214 302 381 2207
Fragments arranged by size:
2207 1125 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2521 2575 4059 4224 50
Size: 2471 54 1484 165 55
Fragments arranged by size:
2471 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 309 2141 309
Size: 1832 2397
BsrFI r'CCGG_y
Cuts at: 298 2227 2408 2617 298
Size: 1929 181 209 1910
Fragments arranged by size:
1929 1910 209 181
BssSI C'ACGA_G
Cuts at: 2500 2858 2500
Size: 358 3871
BstAPI GCAn_nnn'nTGC
Cuts at: 1064 1606 1064
Size: 542 3687
BstBI TT'CG_AA
Cuts at: 2590 2590
Size: 4229
BstEII G'GTnAC_C
Cuts at: 1092 1092
Size: 4229
BstXI CCAn_nnnn'nTGG
Cuts at: 741 904 741
Size: 163 4066
BstYI r'GATC_y
Cuts at: 1 1254 1721 1744 2079 2325 3337 3423 3435
Size: 1253 467 23 335 246 1012 86 12
Cuts at: 3435 3781 1
Size: 346 449
Fragments arranged by size:
1253 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 568 568
Size: 4229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 390 2179 2360 2523 390
Size: 1789 181 163 2096
Fragments arranged by size:
2096 1789 181 163
BtsI GCAGTG_nn'
Cuts at: 1472 1472
Size: 4229
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4229
DrdI GACnn_nn'nnGTC
Cuts at: 1935 2793 1935
Size: 858 3371
EaeI y'GGCC_r
Cuts at: 296 449 1205 1814 1988 2379 2406 3751 296
Size: 153 756 609 174 391 27 1345 774
Fragments arranged by size:
1345 774 756 609 391 174 153 27
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4229
EarI CTCTTCn'nnn_
Cuts at: 974 1171 2252 2462 974
Size: 197 1081 210 2741
Fragments arranged by size:
2741 1081 210 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1024 2437 2747 2893 4039 1024
Size: 1413 310 146 1146 1214
Fragments arranged by size:
1413 1214 1146 310 146
EcoICRI GAG'CTC
Cuts at: 378 1241 378
Size: 863 3366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 653 1165 2054 2486 3233 3501 223
Size: 430 512 889 432 747 268 951
Fragments arranged by size:
951 889 747 512 432 430 268
EcoO109I rG'GnC_Cy
Cuts at: 865 1368 865
Size: 503 3726
EcoRI G'AATT_C
Cuts at: 431 1233 431
Size: 802 3427
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 717 991 2248 717
Size: 274 1257 2698
Fragments arranged by size:
2698 1257 274
FspI TGC'GCA
Cuts at: 2010 2010
Size: 4229
HaeII r_GCGC'y
Cuts at: 242 1663 1911 2933 3912 242
Size: 1421 248 1022 979 559
Fragments arranged by size:
1421 1022 979 559 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 183 215 389 421 2071 2101 2103 2133 183
Size: 32 174 32 1650 30 2 30 2279
Fragments arranged by size:
2279 1650 174 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1268 1268
Size: 4229
KasI G'GCGC_C
Cuts at: 238 1659 1907 238
Size: 1421 248 2560
Fragments arranged by size:
2560 1421 248
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 458 980 2900 3084 458
Size: 522 1920 184 1603
Fragments arranged by size:
1920 1603 522 184
MscI TGG'CCA
Cuts at: 451 1207 1990 451
Size: 756 783 2690
Fragments arranged by size:
2690 783 756
MslI CAynn'nnrTG
Cuts at: 739 1121 1192 2218 2345 739
Size: 382 71 1026 127 2623
Fragments arranged by size:
2623 1026 382 127 71
MspA1I CmG'CkG
Cuts at: 280 387 1006 1055 1340 1654 2014 3027 3272
Size: 107 619 49 285 314 360 1013 245
Cuts at: 3272 280
Size: 1237
Fragments arranged by size:
1237 1013 619 360 314 285 245 107
49
NaeI GCC'GGC
Cuts at: 300 2410 300
Size: 2110 2119
NarI GG'CG_CC
Cuts at: 239 1660 1908 239
Size: 1421 248 2560
Fragments arranged by size:
2560 1421 248
NgoMIV G'CCGG_C
Cuts at: 298 2408 298
Size: 2110 2119
NspI r_CATG'y
Cuts at: 1282 1782 2689 1282
Size: 500 907 2822
Fragments arranged by size:
2822 907 500
PciI A'CATG_T
Cuts at: 2685 2685
Size: 4229
PflMI CCAn_nnn'nTGG
Cuts at: 649 649
Size: 4229
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 4197
PspXI vC'TCGA_Gb
Cuts at: 1128 1128
Size: 4229
PstI C_TGCA'G
Cuts at: 1276 1961 1276
Size: 685 3544
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 387 1654 2014 387
Size: 1267 360 2602
Fragments arranged by size:
2602 1267 360
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4229
SacI G_AGCT'C
Cuts at: 380 1243 380
Size: 863 3366
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 1171 2252 2462 1171
Size: 1081 210 2938
Fragments arranged by size:
2938 1081 210
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 3746 3746
Size: 4229
SfcI C'TryA_G
Cuts at: 33 1272 1957 2950 3141 33
Size: 1239 685 993 191 1121
Fragments arranged by size:
1239 1121 993 685 191
SfoI GGC'GCC
Cuts at: 240 1661 1909 240
Size: 1421 248 2560
Fragments arranged by size:
2560 1421 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4229
SmlI C'TyrA_G
Cuts at: 1128 1384 2299 2791 3053 1128
Size: 256 915 492 262 2304
Fragments arranged by size:
2304 915 492 262 256
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 1282 1782 1282
Size: 500 3729
StyI C'CwwG_G
Cuts at: 554 803 861 1220 1363 1717 554
Size: 249 58 359 143 354 3066
Fragments arranged by size:
3066 359 354 249 143 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 773 1812 1945 2404 2613 773
Size: 1039 133 459 209 2389
Fragments arranged by size:
2389 1039 459 209 133
TatI w'GTAC_w
Cuts at: 1154 3744 1154
Size: 2590 1639
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4197
Tth111I GACn'n_nGTC
Cuts at: 2026 2026
Size: 4229
XbaI T'CTAG_A
Cuts at: 288 1260 288
Size: 972 3257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1724 1724
Size: 4229
XhoI C'TCGA_G
Cuts at: 1128 1128
Size: 4229
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4229
ZraI GAC'GTC
Cuts at: 575 575
Size: 4229
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI